• Title/Summary/Keyword: LMP2A

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ASCL2 Gene Expression Analysis and Its Association with Carcass Traits in Pigs

  • Cheng, H.C.;Zhang, F.W.;Deng, C.Y.;Jiang, C.D.;Xiong, Y.Z.;Li, F.E.;Lei, M.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.10
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    • pp.1485-1489
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    • 2007
  • Achaete-scute like 2 (ASCL2) gene encodes a member of the basic helix-loop-helix transcription factor which is essential for the maintenance of proliferating trophoblasts during placental development. ASCL2 gene preferentially expresses the maternal allele in the mouse. However, it escapes genomic imprinting in the human. In this study, the complete open reading frame consisting of 193 amino acids of ASCL2 gene was obtained. Sequence analysis indicated that a C-G mutation existed in the 3' region between Meishan and Large White pigs. The polymorphism was used to determine the monoallelic or biallelic expression with RT-PCR-RFLP in pigs of Large $White{\times}Meishan$ $F_1$ hybrids. Imprinting analysis indicated that the ASCL2 gene expression was biallelic in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). PCR-RFLP was used to detect the polymorphism in 270 pigs of the "$Large\;White{\times}Meishan$" $F_2$ resource population. The statistical results showed highly significant associations of the genotypes and fat meat percentage (FMP), lean meat percentage (LMP) and ratio of lean to fat (RLF) (p<0.01), and significant associations of the genotypes and loin eye area (LEA) and internal fat rate (IFR) (p<0.05).

A Study on the Variation of Mechanical Properties Due to Thermal Aging in 2.25Cr-1Mo Boiler Tube Steel (2.25Cr-1Mo 강의 열화와 기계적 성질변화에 관한 연구)

  • Jeong, Hee-Don
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.20 no.5
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    • pp.1372-1381
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    • 1996
  • As recieved boiler tuve steel was aged artificially at $650^{\circ}C$ and$690^{\circ}C$ for various time duration to simulate the material deterioration which could be occurred during the operation of fossiol power plants. And the tensile tests, the microhardness tests and the characterization of carbides formed in the aging process were performed to asses the relationship between the mechanical properties and the effect of thermal aging. Furthernore, the amout of Mo-rich carbide were investigated by ondestructive method by noticing the fact that formation of Mo-rich carbide were investigated by ondestructive melthod by noticing the fact that formation of Mo-rich carbides($Mo_6C$) which stabilizes lastly affects the mechanical properties. It was known that the microhardness results of service exposed materials were similar to the ones which are aged at $650^{\circ}C$. The room temperature measurement showed small variation in the yield points and ultimate strength in materials aged at $650^{\circ}C$. Those properties at $540^{\circ}C$ showed the abrupt decrease compared with as received material even if short aging time. And it was found that $650^{\circ}C$ $690^{\circ}C$ aging cause different effects on mechanical properties, although the temperature time parameters(LMP;Larson-Miller parameter) are same. And it was concluded that the aigng at $650^{\circ}C$ is more appropriate to simulate the service exposed condition. Finally, the relationship between high temperature tensile properties and Ip values were established, which offers a potential way of reliability tests onthe power plant components.

Degradation Degree Evaluation of Heat Resisting Steel by Electrochemical Technique Part 2 : Effect of Testing Conditions on Evaluation Value of Degradation Degree and Changes of Mechaical Properties (전기화학적 방법에 의한 내열강의 열화도측정 제2보 : 열화도측정치에 미치는 측정조건들의 영향과 기계적성질 변화에 대해서)

  • 정희돈;권영각;장래웅
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.17 no.2
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    • pp.300-312
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    • 1993
  • The material deterioration of service-exposed boiler tube steels in fossil power plant was evaluated by using the electrochemical technique namely, modified electrochemical potentiokinetic reactivation(EPR). It was focused that the passivation of Mo$_{6}$C carbide which governs the mechanical properties of Mo alloyed steels did not occur even in the passivity region of steel in sodium molybdate solution and the reactivation peak current (Ip) observed as the result of non-passivation indicating the precipitation of Mo$_{6}$C carbides. To obtain the optimal test conditions for the field test by using the specially designed electrochemical cell, the effects of scan rate, the surface roughness and the pH of electrolyte on Ip value were also investigated. Furthermore, the change of mechanical properties occurred during the long time exposure at high temperature was evlauated quantitatively by small punch(SP) tests and micro hardness test taking account of the metallurgical changes. It is known that reactivation peak current (Ip) has a good relationship with Larson-Miller Parameter(LMP) which represents the information about material deterioration occurred at high temperature environment. In addition it was possible to estimate the ductile-brittle transition temperature (DBTT) by means of the SP test. The Sp test could be, therefore, suggested as a reliable test method for evaluating the material degradation of boiler tube steels. From the good correaltion between the SP DBTT and Ip values shown in this study, it was knows that the change of mechanical properties could be evaluated non-destructively by measurring only Ip values.ues.

High Prevalence of the China 1 Strain of Epstein-Barr Virus in Korea as Determined by Sequence Polymorphisms in the Carboxy-Terminal Tail of LMP1

  • Cho, Sung-Gyu;Lee, Won-Keun
    • Journal of Microbiology
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    • v.41 no.2
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    • pp.129-136
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    • 2003
  • The Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMPI) exhibits considerable sequence heterogeneity among EBV isolates. Seven distinct EBV strains have been defined based on sequence polymorphisms in the LMPI gene, which are designated China 1, China 2, China 3, Alaskan, Mediterranean, NC, and the B95-8 strains. In this study, we analyzed a 30-bp deletion and sequence variations in the carboxy-terminal region of the LMPl gene in 12 EBV isolates from spontaneous lym-phoblastoid cell lines derived from individuals with non-EBV associated cancers in Korea. Eleven of the 12 isolates showed a 30-bp deletion spanning LMPI amino acids 342 to 353, suggesting a high prevalence of the LMPI 30-bp deletion variant among EBV isolates in Korea. In addition, all 12 isolates had a 15-bp common deletion in the 33-bp repeat region and multiple base-pair changes relative to the prototype B95-8 EBV strain along with variations in the number of the 33-bp repeats. The bp changes at positions 168746, 168694, 168687, 168395, 168357, 168355, 168631, 168320, 168308, 168295, and 168225 were highly conserved among the isolates. Comparative analysis of sequence change patterns in the LMPI carboxy-terminal coding region identified nine 30-bp deletion variants as China 1, two deletion variants as a possible interstrain between the Alaskan and China 1 strains, and a single undeleted variant as a possible variant of the Alaskan strain. These results suggest the predominance of the China 1 EBV strain in the Korean population.

A Cross-Sectional Study of Nutrient Intakes by Gestational Age and Pregnancy Outcome(I) (우리나라 임신부의 임신 시기별 영양 섭취상태 및 임신결과에 대한 횡적 조사 연구(I))

  • 유경희
    • Journal of Nutrition and Health
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    • v.32 no.8
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    • pp.877-886
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    • 1999
  • To assess the effect of an antenatal nutritional status on pregnancy outcome, especially neonatal birty weight, one-day 24hr-recall and two-day recording methods for dietary survey and interview for general and obstetric characteristics of each subject were completed and pregnancy outcome was recorded by phone after delivery. 147 pregnant women attending routinely public health centers in Ulsan were divided into 1st trimester(n=36), 2nd trimester(n=102), 3rd trimester(n=71) by LMP(Last Menstrual Period) because some subjects attended repeatedly in different trimester. The subjects were aged 27.9$\pm$2.9 as mean and the level of education was senior high school and more. 20.4% of subjects experienced spontaneous abortion and 30.0% experienced induced abortion in previous pregnancy. Mean intakes of all nutrients except ascorbic acid were significantly different but dietary composition of energy intakes was not different between trimester. Mineral of calcium, iron and zinc did not meet the RDA for pregnancy outcome was about 20%, which consists of spontaneous abortion (3.4%), caesarian section(15.6%), premature delivery(0.7%) and still births(0.7%). The mean birth weight of neonates is 3.31kg the rate of neonatal birth weight below 10th percentile was 8.4% and the rate of low birth weight(<2.5kg) was 3.1%. By analysis of nutrient factors that influence on the neonatal birth weight (NBW), iron intake correlated negatively and zinc intake correlated positively with NBW in 1st trimester but fat and iron intakes correlated with NBW positively in 3rd trimester. Prepregnancy weight, gestational age at delivery and No. of induced abortion had a positive effects on NBW and No. of spontaneous abortion and te severity of morning sickness had a negative effects on NBW.

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Liquid Membrane Permeation of Nitrogen Heterocyclic Compounds Contained in Model Coal Tar Fraction

  • Kim, Su-Jin;Kang, Ho-Cheol;Kim, Yong-Shik;Jeong, Hwa-Jin
    • Bulletin of the Korean Chemical Society
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    • v.31 no.5
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    • pp.1143-1148
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    • 2010
  • We investigated the separation of nitrogen heterocyclic compound (NHC) contained in a model coal tar fraction comprising four kinds of NHC [indole (In), quinoline (Q), iso-quinoline (iQ), quinaldine (Qu)], three kinds of bicyclic aromatic compound (BAC) [1-methylnaphthalene (1MN), 2-methylnaphthalene (2MN), dimethylnaphthalene (DMN) mixture with ten structural isomers (DMNs; regarded as one component)], biphenyl (Bp) and phenyl ether (Pe) by liquid membrane permeation (LMP). A batch-stirred tank was used as the permeation unit. An aqueous solution of saponin and n-hexane were used as the liquid membrane and the outer oil phase, respectively. Yield and selectivity of individual NHC was much larger than that of BAC, Bp and Pe. Increasing the initial mass fraction of the saponin to the membrane solution ($C_{sap,0}$) and the initial volume fraction of O/W emulsion to total liquid in a stirred tank (${\phi}_{OW,0}$) resulted in deteriorating the yield of individual NHC, but increasing the stirring speed (N) resulted in improving the yield of each NHC. With increasing $C_{sap,0}$, the selectivity of each NHC based on DMNs increased. Increasing ${\phi}_{OW,0}$ and N resulted in decreasing the selectivity of individual NHC based on DMNs. At an experimental condition fixed, the sequence of the yield and selectivity in reference to DMNs for each NHC was Q > Qu = iQ > In. Furthermore, we compared LPM method with methanol extraction method in view of the separation efficiency (yield, selectivity) of NHC.

The Analysis of Creep characteristics for Turbine blade using Theta projection method (θ 투영법을 이용한 터빈 블레이드의 크리프 특성 분석)

  • Lee, Mu-Hyoung;Han, Won-Jae;Jang, Byung-Wook;Lee, Bok-Won;Park, Jung-Sun
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.39 no.4
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    • pp.321-331
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    • 2011
  • The present work is aimed to analyze the creep characteristics of a turbojet engine turbine blade using the theta projection method. The theta projection method has been widely used due to its advantages and flexibility. For the creep characteristic analysis of the turbine blade, tests are performed considering the operating conditions and the non-linear material properties. Results from the creep test are fitted using the four theta model. The predicted proprieties using the four theta model are compared with the prediction model and creep test results. To obtain an optimum value of the four theta parameters in non-linear square method, a number of computing processes in the non-linear least square method were carried out to obtain full creep curves. Results using the theta model has more than 0.95 value of $R^2$. The results between the experimental values and predicted four theta model has about 90.0% accuracy. The theta projection method can be utilized for a design purpose to predict the creep behavior.

The Development of Material Separation Technique for Recycling of Waste Car Tail Lamp (폐자동차(廢自動車) Tail Lamp의 재활용(再活用)을 위한 재질분리(材質分離) 기술개발(技術開發))

  • Jeon, Ho-Seok;Park, Chul-Hyun;Baek, Sang-Ho;Park, Jai-Koo
    • Resources Recycling
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    • v.15 no.6 s.74
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    • pp.25-32
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    • 2006
  • In this study, waste car tau lamps that usually gets incinerated or buried has been recovered during the dispose process in order to increase the recycling rate of waste cars and the material separation research was performed by applying triboelectrostatic separation method. After a series of charging characteristic test, PMMA materials was confirmed of being an effective charging material for waste car tail lamp material separation, a cyclone charger using PMMA material for continuous process was developed. In optimum test conditions using triboelectrostatic separator developed in this study, high quality PMMA material with recovery rate of 99.0% and 90.2% was achieved from the waste car tail lamp. Therefore, a material separation technology that can recycle tail lamps of waste cars has been established.

Nondestructive Characterization for Remanent Life of Advanced Ferritic Steel by Reversible Permeability (가역투자율에 의한 첨단 페라이트강의 잔여수명에 대한 비파괴평가)

  • Hong, Seung-Pyo;Ryu, Kwon-Sang;Kim, Chung-Seok
    • Journal of the Korean Society for Nondestructive Testing
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    • v.33 no.2
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    • pp.181-186
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    • 2013
  • We present nondestructive characterization for remanent life of advanced ferritic steels, next-gen energy facility materials by reversible permeability. The reversible permeability is based on the theory that the value of reversible permeability is the same differential of the hysteresis loop. The measurement principle is based on the foundation of harmonics voltage induced in a sensing coil using a lock-in amplifier tuned to the frequency of the exciting one. The peak interval of reversible permeability(PIRP), Vickers hardness, and tensile strength(TS) of the aged samples decreased with aging time. We could estimate the remanent life of advanced ferritic steel by using the relationship between the peak interval of reversible permeability and Larson-Miller parameter(LMP), non-destructively.

Decision-Making of Determining the Start Time of Charging / Discharging of Electrical Vehicle Based on Prospect Theory

  • Liu, Lian;Lyu, Xiang;Jiang, Chuanwen;Xie, Da
    • Journal of Electrical Engineering and Technology
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    • v.9 no.3
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    • pp.803-811
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    • 2014
  • The moment when Electrical Vehicle (EV) starts charging or discharging is one of the most important parameters in estimating the impact of EV load on the grid. In this paper, a decision-making problem of determining the start time of charging and discharging during allowed period is proposed and studied under the uncertainty of real-time price. Prospect theory is utilized in the decision-making problem of this paper for it describes a kind of decision making behaviors under uncertainty. The case study uses the parameters of Springo SGM7001EV and adopts the historical realtime locational marginal pricing (LMP) data of PJM market for scenario reduction. Prospect values are calculated for every possible start time in the allowed charging or discharging period. By comparing the calculated prospect values, the optimal decisions are obtained accordingly and the results are compared with those based on Expected Utility Theory. Results show that with different initial State-of-Charge ($SoC_0$) and different reference points, the optimal start time of charging can be the one between 12 a.m. to 3 a.m. and optimal discharging starts at 2 p.m. or 3p.m. Moreover, the decision results of Prospect Theory may differ from that of the Expected Utility Theory with the reference points changing.