• 대한전자공학회논문지SD
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• 제39권12호
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• pp.1016-1021
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• 2002

LCD backlight용 형광램프(fluorescent lamp, FL)의 전극구조에 따른 발광특성을 평가하였다. 동일한 조건에서 제작된 cold cathode fluorescent lamp(CCFL) 및 EEFL에 대하여 인가전압과 EEFL의 전극 폭에 따른 휘도와 발광효율을 측정, 평가하였다. 12 V에서 측정된 CCFL의 휘도와 발광효율은 각각 27600 cd/㎡ 및 35.3 lm/w를 나타내었다 EEFL의 휘도는 전극면적을 증가시킬수록 증가되었고, 발광효율은 전극 면적이 증가되면서 증가되었다가 전극 폭 20 mm에서 최대값을 나타낸 다음 다시 감소하였다. 20 mm의 전극 폭을 갖는 EEFL에 대하여 12 V와 14 V에서 측정된 휘도와 발광효율은 각각 21600 cd/㎡, 26500 cd/㎡ 및 35.6 lm/w, 34.8 lm/w였다.

• 항공우주
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• 제59권
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• pp.32-35
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• 1998

미국의 에어즈사는 현재 LM-200 로드마스터라는 기종을 개발중에 있다. 에어즈사가 비즈니스용 항공기 메이커여서 LM-200도 역시 비즈니스기지만 그 구조와 성능이 매우 재미있고 용도가 매우 광범위할 것으로 보여 이색 항공기의 하나로 소개하고자 한다.

• 한국응용과학기술학회지
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• 제35권4호
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• pp.1250-1259
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• 2018

여주(Momordica charantia) 열수 추출물을 젖산균(Leuconostoc mesenteroidesies)으로 발효하여 얻은 생산물의 항산화 및 항당뇨 효과를 관찰하였다. 여주 발효물(MC-LM)과 여주 추출물(MC)의 항산화 활성은 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical 소거 측정법을 사용하여 관찰하였다. MC 처리군은 radical 소거활성이 거의 나타나지 않았으나 MC-LM의 경우 $500{\mu}g/mL$ 농도에서 47%의 활성이 관찰되었으며 MC-LM의 농도가 증가함에 따라 활성이 유의하게(p<0.05) 증가하는 것을 확인 할 수 있었다. MC-LM은 MC에 비해 peroxyl radical에 의한 DNA의 산화적 손상을 더 효과적으로 억제하였으며 MC-LM의 농도가 증가함에 따라 strand breakage의 억제가 유의적으로 증가 하였다(p<0.05). 혈당량과 밀접한 관련이 있는 ${\alpha}-glucosidase$의 활성 억제정도를 측정한 결과 MC는 8 mg/mL 농도에서 ${\alpha}-glucosidase$의 활성을 30.1% 감소시켰고 같은 농도에서 MC-LM은 58.9% 감소 시켰다. 또한 alloxan에 의한 RIN-m5F 췌장세포 사멸에 발효물의 영향을 관찰한 결과 MC-LM을 농도별로 처리한 군에서 세포생존율이 유의적으로(p<0.05) 증가하였으며 $1000{\mu}g/mL$ 농도에서 MC군 보다 20% 높게 증가되었다. Alloxan에 의한 ${\beta}-cell$파괴를 유도하여 RIN-m5F cell이 분비한 인슐린의 측정결과 MC-LM을 농도별로 첨가한 모든 군에서 인슐린 분비능이 증가하는 경향을 보였으며, $1000{\mu}g/mL$ 농도에서 MC군 보다 15% 높게 증가되었다. 결론적으로 본 연구에서 관찰된 결과들을 통해 여주 발효물은 항산화 및 항당뇨 효과가 있는 것으로 확인되었다.

• 한국식품영양학회지
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• 제35권5호
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• pp.332-342
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• 2022

Nonalcoholic fatty liver disease (NAFLD) is recognized one of the leading metabolic diseases globally, and the younger age population with the disease is rapidly growing, especially in developed countries. Since there has been no approved medicine, losing weight is known to be the only best remedy to control or reverse the disease. Recently, the field of microbiome has attracted much attention to offer more practical choices for patients. Here, we provide experimental evidence that Streptococcus thermophilus LM1012 (LM1012), a safe probiotic strain, is effective for improving NAFLD indexes. In the methionine-choline deficient (MCD) diet induced C57BL/6 mouse model, administration of LM1012 promoted marked reductions of aspartate transaminase (23.8%), total bilirubin (27.8%), hydroxycholesterol (64.2%), triglyceride (29.7%) and IL-1β (68.3%) compared to the MCD diet alone group. Also, the histopathological data imply that LM1012 inhibited fat accumulation and inflammation in the liver, which are the key biomarkers for progression of the disease. Together, these findings suggest that human consumption of LM1012 as a healthy nutritional supplement, may be helpful in reducing the risk of liver damages in NAFLD patients.

• 대한치과보철학회지
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• 제35권2호
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• pp.373-384
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• 1997

In this study, 4 types of dowel pin were used to fabricate removable dies using the Pindex system. The comparison of interabutment distances between before and after sawing were conducted in each type of dowel pin, and the horizontal discrepancies in the interabutment distance according to the abutment location and the type of dowel pin were comparatively analyzed. Abutments LM(left molar), LP(left premolar), A(anterior), RP(right premolar), and RM (right molar) were used for measurement. The interabutment distances were measured between each abutment : LM-LP, LM-A, LM-RP, and LM-RM. The slide scanned image of each model was magnified on the computer screen and the measurements were conducted using the meeting point of the cross on the occlusal surface of each abutment. The results were as follows : 1. In the comparison of interabutment distances between before and after sawing in each type of dowel pin, there was no significant difference in World dowel pin(double), Bi-pin(double), Maxcel dowel pin(single). In the World dowel pin(single) there was no significant difference between LM-LPs, but in the other measurements (LM-As, LM-RPs, and LM-RMs) there was significant difference between before and after sawing(p<0.05). 2. In comparison of horizontal discrepancies of the measurement according to the abutment location, there was significant difference between measurement LM-LP($0.27{\pm}0.19mm$) and the others : LM-A($0.46{\pm}0.20mm$), LM-RP($0.38{\pm}0.25mm$), LM-RM($0.45{\pm}0.22mm$) (p<0.05). 3. In comparison of horizontal discrepancies of the measurement according to the type of dowel, there was no significant difference between each dowel. As a result when fabricating a removable die using the previously mentioned 4 types of dowel pins, it is thought that the horizontal discrepancy increased in proportion to the interabutment span length and does not depend on the type of dowel pin.

• 한국어병학회지
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• 제35권2호
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• pp.195-203
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• 2022

본 연구는 lincomycin (LM)의 근육투여에 따른 저수온(13℃)에서의 잔류 특성 및 넙치에서 병원성 세균으로 발견되는 그람음성 세균에서의 시험관 내 in vitro 약효를 평가하기 위하여 수행하였다. LM을 10, 20 및 40 mg/kg의 용량으로 단회 투여하였을 때, 근육내 농도가 잔류허용기준인 0.1 mg/kg 이하로 감소하는 기간은 각각 32일, 33일 및 55일로 평가되었다. 또한, 현장 분리 균주 그람음성 세균인 Edwardsiella piscida 및 Vibrio harveyi에 대하여 LM의 minimal inhibitory concentration (MIC)로 평가된 in vitro 약효는 각각 >256 ㎍/mL 및 32 ㎍/mL을 나타냈다. 연구 결과, 넙치 양식장에서 사용되는 LM의 임상용량인 10 mg/kg을 고려하였을 때의 휴약기간을 30일 이상으로 설정하면 합리적일 것으로 판단된다. 또한 LM이 넙치 연쇄상구균증 치료 목적으로 투여될 때 그 동시에 감염된 그람음성 세균에는 약효가 발휘되지 않을 것으로 추정한다.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.598-609
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• 2017

Pediococcus pentosaceus ID-7 was isolated from kimchi, a Korean fermented food, and it showed high activity for lactose hydrolysis. The ${\beta}$-galactosidase of P. pentosaceus ID-7 belongs to the GH2 group, which is composed of two distinct proteins. The heterodimeric LacLM type of ${\beta}$-galactosidase found in P. pentosaceus ID-7 consists of two genes partially overlapped, lacL and lacM encoding LacL (72.2 kDa) and LacM (35.4 kDa). In this study, Escherichia coli MM294 was used for the production of LacL, LacM, and LacLM. These three types of recombinant proteins were expressed, purified, and characterized. The specific activities of LacLM and LacL were 339 and 31 U/mg, respectively. However, activity was not detected with LacM alone. The optimal pH of LacLM and LacL was pH 7.5 and pH 7.0, and the optimal temperature of LacLM and LacL was $40^{\circ}C$ and $50^{\circ}C$, respectively. The optimal temperature changes indicate that LacLM is able to achieve higher activity at a relatively lower temperature. LacLM was strongly activated by $Mg^{2+}$, $Mn^{2+}$, and $Zn^{2+}$, which was not true for LacL. Consistent with this, EDTA strongly inactivated LacLM and LacL, but the presence of reducing agents did not dramatically alter the activity. Taken together, multiple alignment of amino acid sequences and phylogenetic analysis results of LacL and LacM of P. pentosaceus ID-7 suggest the evolution of LacL into LacLM and that the use of divalent metal ions results in higher activity.

• 전산구조공학
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• 제12권1호
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• pp.35-43
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• 1999

• 한국농공학회논문집
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• 제57권1호
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• pp.25-35
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• 2015

As livestock manure treatment is becoming a problem, manure application in forest plantation is recommended as an alternative. In this study, to investigate the impact due to liquid manure application in forest plantation, soil, soil water and shallow groundwater quality had been monitored in poplar experimental site where the liquid manure (LM) was applied. Water samples were collected weekly during growing season (April to October) from 2008 to 2011. From the monitoring results, phosphorus concentration in the soil and soil water had no significant difference between LM and control plots. $NO_3$-N concentration of soil water in LM, however, showed higher concentration (13.6 mg/l at 40 cm, 35.1 mg/l at 80 cm) than control plot (1.5 mg/l at 40 cm, 0.5 mg/l at 80 cm). In case of shallow groundwater quality, pH, heavy metal, etc. were satisfied to the national agricultural water quality standard of groundwater and there were no significant difference between upstream and downstream. The $NO_3$-N concentration of shallow groundwater was also not exceeded the national drinking water standard. However, $NO_3$-N concentration in soil water and downstream of shallow groundwater had increased in 2011 when non-composted LM was applied mostly in non-growing season of tree (September). From the results, it is important to control nitrogen source, application time and decomposed or not when LM is applied. In addition, to investigate nitrate source, further long-term monitoring and modelling could be necessary.

• 한국축산식품학회지
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• 제35권5호
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• pp.692-702
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• 2015

Lactobacillus mucosae is a natural resident of the gastrointestinal tract of humans and animals and a potential probiotic bacterium. To understand the global protein expression profile and metabolic features of L. mucosae LM1 in the early stationary phase, the QExactive^TM Hybrid Quadrupole-Orbitrap Mass Spectrometer was used. Characterization of the intracellular proteome identified 842 proteins, accounting for approximately 35% of the 2,404 protein-coding sequences in the complete genome of L. mucosae LM1. Proteome quantification using QExactive^TM Orbitrap MS detected 19 highly abundant proteins (> 1.0% of the intracellular proteome), including CysK (cysteine synthase, 5.41%) and EF-Tu (elongation factor Tu, 4.91%), which are involved in cell survival against environmental stresses. Metabolic pathway annotation of LM1 proteome using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that half of the proteins expressed are important for basic metabolic and biosynthetic processes, and the other half might be structurally important or involved in basic cellular processes. In addition, glycogen biosynthesis was activated in the early stationary phase, which is important for energy storage and maintenance. The proteogenomic data presented in this study provide a suitable reference to understand the protein expression pattern of lactobacilli in standard conditions