• 전기전자학회논문지
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• 제27권3호
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• pp.355-367
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• 2023

Local Interconnect Network(LIN)은 저속의 차량 통신 버스로 윈도우, 도어, 공조, 조명 등 바디 어플리케이션에 널리 사용되고 있다. 본 논문에서는 LIN 버스의 프로토콜과 메시지 프레임에 대해 자세히 설명한다. LIN 버스에서는 기본적으로 메시지 프레임에서 ID와 페이로드만 전송하며, 이들 ID와 페이로드를 해석하는 방법은 LIN Description File(LDF)를 통해서 각각의 LIN 버스마다 다르게 정의한다. 본 논문에서는 LDF의 문법과 예제에 대해서도 자세히 설명한다.

• 전기전자학회논문지
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• 제20권3호
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• pp.333-336
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• 2016

차량 내 전자장치에서 효과적인 서브 버스로서 표준화된 저속 직렬 통신 프로토콜인 LIN(local interconnect network)이 개발되었다. 본 논문에서는 LIN 버전 2.2A를 기반으로 LIN 제어기를 Verilog HDL을 이용하여 구현하였다. 구현된 LIN 제어기는 FPGA에서 동작을 확인하였으며 IP 형태로 제공되어 SoC 시스템에 통합이 가능하다. 0.18um 공정에서 합성하였을 때의 게이트 수는 약 2,300 게이트이다.

• 한국자동차공학회논문집
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• 제13권6호
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• pp.48-55
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• 2005

In this paper, a mathematical model and a simulation method for the response time analysis of Local Interconnect Network(LIN) based network systems are proposed. Network-induced delays in a network based control system can vary widely according to the transmission time of message and the overhead time of transmission. Therefore, in order to design a distributed control system using LIN network, a method to predict and verify the timing behavior of LIN protocol is required at the network design phase. Furthermore, a simulation environment based on a timing model of LIN protocol is beneficial to predict the timing behavior of LIN. The model equation is formulated with six timing parameters deduced from timing properties of LIN specification. Additionally, LIN conformance test equations to verify LIN device driver are derived with timing constraints of the parameters. The proposed model equation and simulation method are validated with a result that is measured at real LIN based network system.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.23-30
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• 2019

Objective: Recent studies have demonstrated that lin-28 homolog B (LIN28B)/miRNA let-7 (let-7) plays a role in the regulation of pubertal onset in mammals. However, the role of LIN28B/let-7 in the onset of ovine puberty remains unknown. We cloned the Duolang sheep Lin28B cDNA sequence, detected the expression change of LIN28B, let-7a and let-7g in hypothalamus, pituitary and ovary tissues at three different pubertal stages. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of LIN28B gene from Duolang sheep and the bioinformatics methods were applied to analyze the amino acid sequence of LIN28B protein. The mRNA expression levels of the LIN28B gene at different pubertal stages were examined by real time RT-PCR. Results: LIN28B cDNA of Duolang sheep was cloned, and two transcripts were obtained. The amino acid sequence of transcript 1 shares 99.60%, 98.78%, and 94.80% identity with those of goat, wild yak and pig, respectively. Strong LIN28B mRNA expression was detected in the hypothalamus, pituitary, ovary, oviduct and uterus, while moderate expression was found in the liver, kidney, spleen and heart, weak expression was observed in the heart. No expression was found in the lungs. Quantitative real-time PCR (QPCR) and western-blot analysis revealed that the LIN28B was highly expressed in the hypothalamus and ovary at prepuberty stages, and this expression significantly decreased from the prepuberty to puberty stages (p<0.05). Markedly increased levels of mRNA expression were detected in the pituitary from prepuberty to puberty (p<0.05) and then significantly decreased from puberty to post-puberty (p<0.05). The expression levels of let-7a and let-7g showed no significant changes among different pubertal stages (p>0.05). Conclusion: These results provided a foundation for determining the functions of LIN28B/let-7 and their role in the onset of sheep puberty.

• Journal of Plant Biotechnology
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• 제30권1호
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• pp.103-108
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• 2003

본 연구는 난분해성 농약의 자연분해를 유도하기 위하여 ${\gamma}$-BHC를 탄소원으로 이용하여 호기적으로 자화하는 미생물 (Sphingomonas paucimobilis)에서 분리된 탈염소화효소 중 ${\gamma}$-BHC분해의 첫단계에 관여하는 linA유전자를 담배에 도입하여 BHC에 활성을 나타내는 형질전환 시물체를 만들었다. LinA 유전자를 함유한 pJK 108 백터를 제작하여. Agrobacterium를 이용한 leaf disk transformation 방법으로 담배에 형질전환시켰으며, northern분석 및 Southern분석을 통해 형질전환 개체에서 linA유전자의 도입과 linA유전자 유래의 mRNA가 전사됨을 확인하였다. Western 분석에서 linA단백질의 발현을 확인하였고, gas chromatography를 이용하여 담배 형질전환 개체에서 BHC가 ${\gamma}$-PCCH와 1,2.4-TCB로 분해되는 것을 확인하였다.

• 정보보호학회논문지
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• 제33권2호
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• pp.325-336
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• 2023

자율주행 기술이 발전함에 따라 차량에 탑재되는 외부접점 센서의 수가 증가하고 있으며 특히 차량용 초음파 센서는 버스 토폴로지 형태로서 LIN 프로토콜을 사용하여 연결되며 주변을 감지하고 상태 메시지를 전송하여 차량 내부 네트워크에 접근한다. LIN은 프로토콜의 보안성이 검증되지 않아 공격에 취약하기 때문에 안전성을 평가하기 위한 테스트가 필요하지만, 물리적인 제약으로 인해 실제 차량을 이용한 분석에 한계가 있으며 이를 위한 테스트 환경이 부족하기 때문에 테스트에 어려움이 있다. 따라서, 본 논문에서는 LIN 프로토콜 분석 및 테스트를 위해 CAN과의 연동 분석이 가능한 테스트베드를 개발하고 초음파 센서를 이용하여 그 효용성을 검증하였다.

• Clinical and Experimental Reproductive Medicine
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• 제39권2호
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• pp.87-93
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• 2012

Objective: Lin28 has been known to control the proliferation and pluripotency of embryonic stem cells. The purpose of this study was to determine the downstream effectors of Lin28 in mouse embryonic stem cells (mESCs) by RNA interference and microarray analysis. Methods: The control siRNA and Lin28 siRNA (Dharmacon) were transfected into mESCs. Total RNA was prepared from each type of transfected mESC and subjected to reverse transcription-polymerase chain reaction (RT-PCR) analysis to confirm the downregulation of Lin28. The RNAs were labeled and hybridized with an Affymetrix Gene-Chip Mouse Genome 430 2.0 array. The data analysis was accomplished by GenPlex 3.0 software. The expression levels of selected genes were confirmed by quantitative real-time RT-PCR. Results: According to the statistical analysis of the cDNA microarray, a total of 500 genes were altered in Lin28-downregulated mESCs (up-regulated, 384; down-regulated, 116). After differentially expressed gene filtering, 31 genes were selected as candidate genes regulated by Lin28 downregulation. Among them, neuropeptide Y5 receptor and oocyte-specific homeobox 5 genes were significantly upregulated in Lin28-downregulated mESCs. We also showed that the families of neuropeptide Y receptor (Npyr) and oocyte-specific homeobox (Obox) genes were upregulated by downregulation of Lin28. Conclusion: Based on the results of this study, we suggest that Lin28 controls the characteristics of mESCs through the regulation of effectors such as the Npyr and Obox families.

• 대한한의학방제학회지
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• 제20권2호
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• pp.117-136
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• 2012

This thesis is the study on GuiLinGuBen "ShangHanZaBingLun"'s prescriptions which are totally 324, and unique 91 which are not written in current "ShangHanZaBingLun" and "JinKuiYaoLue", only in GuiLinGuBen "ShangHanZaBingLun". For modern clinacal effectiveness of GuiLinGuBen "ShangHanZaBingLun", this thesis clarifies the prescriptions' distinction between GuiLinGuBen, "ShangHanZaBingLun" and "JinKuiYaoLue". First it classifies prescriptions into 4 groups, which are only in GuiLinGuBen, in "ShangHanZaBingLun", in "JinKuiYaoLue", and in both "ShangHanZaBingLun" and "JinKuiYaoLue". Second it tabulates and describes in detail GuiLinGuBen's prescriptions about title, prescription composition, prescription volumetrin, decotion, and dosage. Third it catches distinctive characteristic of GuiLinGuBen's prescriptions by a comparative study which clarifies the differences of prescriptions between GuiLinGuBen, "ShangHanZaBingLun" and "JinKuiYaoLue". Current "ShangHanZaBingLun" and "JinKuiYaoLue"'s remedy focus on Shanghan and Jabbyong, so it has no choice but to have large remedy vaccum. The prescriptions only in GuiLinGuBen have same system with "ShangHanZaBingLun" and "JinKuiYaoLue"'s prescriptions, and contain unique Fever remover(淸熱劑) and Counterbalancer (補劑), so they could give more clinacal practice over "ShangHanZaBingLun" and "JinKuiYaoLue".

• 전력전자학회:학술대회논문집
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• 전력전자학회 2013년도 전력전자학술대회 논문집
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• pp.538-539
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• 2013

LIN(Local Interconnect Network) 통신은 CAN(Controller Area Network) 통신과 더불어 차량용 통신으로 많이 사용되고 있다. LIN 통신은 필요 성능의 수준이 높지 않은 장치 및 시스템이나 비용의 경제적인 측면에서 CAN 통신의 하부버스로 많이 사용되며 LIN 노드에 개수도 점차 늘어나고 있다. 그렇기 때문에 LIN통신으로 시스템 제어 및 모니터링 프로그램을 GUI(Graphic User Interface)로 제작한다면 시스템 상태의 디스플레이 및 모니터링이 수월해진다. 함수와 GUI로 제작되는 LabVIEW는 다른 텍스트 언어기반 개발 프로그램에 비해 설계 시간 단축 및 사후 관리도 쉽다는 장점이 있다. 본 논문에서는 LabVIEW로 제작한 IBS 모듈 기능 검사 장비개발을 통해 LIN 통신으로 송수신되는 데이터를 확인하고 모니터링, 그리고 설계가 용이하다는 것을 소개하고자 한다.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.313-319
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• 2021

Hub cells comprise a niche for germline stem cells and cyst stem cells in the Drosophila testis. Hub cells arise from common somatic gonadal precursors in embryos, but the mechanism of their specification is still poorly understood. Here we find that RNA binding proteins Lin28 and Imp mediate transcript stability of Bowl, a known hub specification factor; Bowl transcripts were reduced in the testis of Lin28 and Imp mutants, and also when RNA-mediated interference against Lin28 or Imp was expressed in hub cells. In tissue culture Luciferase assays involving the Bowl 3'UTR, stability of Luc reporter transcripts depended on the Bowl 3'UTR and required Lin28 and Imp. Our findings suggest that proper Bowl function during hub cell specification requires Lin28 and Imp in the testis hub cells.