• BMB Reports
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• v.51 no.7
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• pp.356-361
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• 2018

Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of $NF-{\kappa}B$ ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKL-mediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data demonstrated that ABLIM1 is a positive regulator of RANKL-mediated osteoclast formation via the modulation of the differentiation and PI3K/Akt/Rac1-dependent motility.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.140-145
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• 2009

Significant attention has recently been drawn to human robot interaction system that uses face detection technology. The most conventional face detection methods have applied under pixel domain. These pixel based face detection methods require high computational power. Hence, the conventional methods do not satisfy the robot environment that requires robot to operate in a limited computing process and saving space. Also, compensating the variation of illumination is important and necessary for reliable face detection. In this paper, we propose the illumination invariant face detection that is performed under the compressed domain. The proposed method uses color balancing module to compensate illumination variation. Experiments show that the proposed face detection method can effectively increase the face detection rate under existing illumination.

• Journal of Korean Elementary Science Education
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• v.24 no.4
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• pp.321-328
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• 2005

In this study the effects of science teaming with the level of inquiry requirement in elementary school science experiment instruction were investigated on cognitive domain. We assigned seventy-three students of the fifth grade into two groups according to the levels of inquiry requirement. After each instruction was implemented, the characteristics of the students' tearning science on cognitive domain were compared and analyzed with the levels of them. The higher level (HL) inquiry-required instruction was more effective in increasing and maintaining the memory on the science teaming than the lower level (LL). Especially, in the aspects of the experimental methods and taking cares which the students engage and perform actively rather than do passively, the memory scores of HL group were higher than those of LL. In addition, the memory scores and the degree of maintenance were higher among students who perceived the instruction as easy and interesting. In conclusion, the HL of instruction could stimulate the students to challenge the problems, thereby make them construct meaning actively and improve the amount and degree of maintenance of memory on science teaming.

• Journal of Korean Elementary Science Education
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• v.38 no.3
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• pp.360-374
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• 2019

The purpose of this study is to investigate the self-perceptions of the elementary school students' on the affective, behavioral and cognitive domains in science class. To see if there are differences in students' perceptions according to grade levels, a self-perception questionnaire was applied to third and fifth grade students in Seoul, Korea. The major results of the study are as follows. First, the perception level of the affective domain was higher than that of the cognitive domain in third grade students. There was no significant difference in the self-perception level of the fifth grade students. Both third and fifth grade students perceived the greatest improvement in the cognitive domain through one year of science teaching. Second, in the life science class, the same tendency was also observed. The students in the third and fifth grade reported that cognitive domains were the most improved through the one-year life science class. Third, when the students' perceptions were compared by grades, the third grade students showed higher self-perception scores than fifth grade students. As the grade increased, the perception scores of the students' lowered. Based on these findings, implications for science education research and teaching and learning at school are discussed.

• Molecules and Cells
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• v.43 no.5
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• pp.469-478
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• 2020

Hepatitis C virus (HCV) propagation is highly dependent on cellular proteins. To identify the host factors involved in HCV propagation, we previously performed protein microarray assays and identified the LIM and SH3 domain protein 1 (LASP-1) as an HCV NS5A-interacting partner. LASP-1 plays an important role in the regulation of cell proliferation, migration, and protein-protein interactions. Alteration of LASP-1 expression has been implicated in hepatocellular carcinoma. However, the functional involvement of LASP-1 in HCV propagation and HCV-induced pathogenesis has not been elucidated. Here, we first verified the protein interaction of NS5A and LASP-1 by both in vitro pulldown and coimmunoprecipitation assays. We further showed that NS5A and LASP-1 were colocalized in the cytoplasm of HCV infected cells. NS5A interacted with LASP-1 through the proline motif in domain I of NS5A and the tryptophan residue in the SH3 domain of LASP-1. Knockdown of LASP1 increased HCV replication in both HCV-infected cells and HCV subgenomic replicon cells. LASP-1 negatively regulated viral propagation and thereby overexpression of LASP-1 decreased HCV replication. Moreover, HCV propagation was decreased by wild-type LASP-1 but not by an NS5A binding-defective mutant of LASP-1. We further demonstrated that LASP-1 was involved in the replication stage of the HCV life cycle. Importantly, LASP-1 expression levels were increased in persistently infected cells with HCV. These data suggest that HCV modulates LASP-1 via NS5A in order to regulate virion levels and maintain a persistent infection.

• Journal of Applied Biological Chemistry
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• v.48 no.3
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• pp.113-119
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• 2005

O-methylation mediated by O-methyltransferases (OMTs) is a common modification in natural product biosynthesis and contributes to diversity of secondary metabolites. OMTs use phenylpropanoids, flavonoids, other phenolics and alkaloids as substrates, and share common domains for S-adenosyl-L-methionine (AdoMet) and substrate binding. We searched Arabiposis genome and found 17 OMTs genes (AtOMTs). AdoMet- and substrate-binding sites were predicted. AdoMet binding domain of AtOMTs is highly conserved, while substrate-binding domain is diverse, indicating use of different substrates. In addition, expressions of six AtOMT genes in response to UV and in different tissues were investigated using real-time quantitative reverse transcriptase-polymerase chain reaction. All the AtOMTs investigated were expressed under normal growth condition and most, except AtOMT10, were induced after UV illumination. AtOMT1 and AtOMT8 were expressed in all the tissues, whereas AtOMT10 showed flower-specific expression. Analysis of these AtOMT gene expressions could provide some clues on AtOMT involvement in the cellular processes.

• The Journal of the Korea Contents Association
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• v.7 no.12
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• pp.273-282
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• 2007

The Domain Name System (DNS) is the core system for managing Internet address resources, providing the most fundamental naming service. Currently, the DNS is classified into a tree structure. In this structure, it is difficult to normally access to the lower DNS, when there is an error in the upper DNS. Such a risk still remains even when a supplementary DNS is operated. However, due to the merit of the DNS enabling fast searches, it is impracticable to abandon the current tree structure. To efficiently correspond to DNS errors, this study suggests a method where the merit of the current tree structure is kept, while a temporary operation of the local DNS is available when errors occur by adding a horizontal and independent DNS structure.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.6
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• pp.635-641
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• 2010

Time-varying channel characteristics in OFDM systems over doubly selective channels cause inter-carrier interferences(ICI) in the frequency domain. Time domain windowing gives rise to restriction on the bandwidth of the frequency domain channel matrix and makes it possible to approximate the OFDM system as a simplified linear input-output model. When successive interference cancellation based on linear MMSE estimation is employed for channel equalization in OFDM systems, symbol detection ordering produces considerable effects on overall system performances. In this paper, we show the reduction of the residual ICI by time domain windowing and the resultant performance improvements, and investigate the effects of SINR- and CSEP-based symbol detection ordering on the performance of successive interference cancellation.

• Korean Journal of Veterinary Research
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• v.39 no.2
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• pp.294-300
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• 1999

In this study PRRSV was isolated from serum of an infected pig and designated as CNV-1, ORF4 gene was sequenced, and the nucleotide sequence, deduced amino acid sequence and the amino acid sequence of the neutralizing domain was compared with other PRRSV Strains. ORF4 gene of the Korean isolate PRRSV CNV-1 was shown to be 537bp in length, which is the same as US strain ISU55 but 21bp longer than another US strain MN1b, and 15bp shorter than European strain LV. The homologies of the nucleotide sequences between the Korean isolate CNV-1 and the US strains ISU55, MN1b and European strain LV were 91.8%, 88.1%, 67.6%, respectively, and the homologies of the deduced amino acid sequences were 94.4%, 84.4%, 68.5%, respectively. The neutralizing domain of the CNV-1 was shown to be 36 amino acids in length which is the same as ISU55, MN1b, but 4 amino acids shorter than that of the neutralizing domain reported in LV. The homologies of the amino acid sequences of the neutralizing domain between the Korean isolate CNV-1 and the US strains ISU55, MN1b and European strain LV were 92.5%, 85%, 57.5%, respectively. The molecular characteristics of ORF4 gene of the Korean isolate PRRSV CNV-1 shown in this study suggests that the CNV-1 is genetically closer to the US strains. Also the wide variation of the neutralizing domain between the CNV-1 and LV suggests that there is substantial immunogenic variation between the two strains.

• Journal of Applied Biological Chemistry
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• v.50 no.3
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• pp.109-114
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• 2007

Sar1p is a ras-related GTP-binding protein that functions in intracellular protein transport between the endoplasmic reticulum (ER) and the Golgi complex. The effector domain of Ras family proteins is highly conserved and this domain is functionally interchangeable in plant, yeast and mammalian Sar1. Using a recombinant Brassica sar1 protein (Bsar1p) harboring point mutations in its effector domain, we here investigated the ability of Sar1p to bind and hydrolyze GTP and to interact with the two sar1-specific regulators, GTPase activating protein (GAP) and guanine exchange factor (GEF). The T51A and T55A mutations impaired Bsar1p intrinsic GTP-binding and GDP-dissociation activity. In contrast, mutations in the switch domain of Bsar1 did not affect its intrinsic GTPase activity. Moreover, the P50A, P54A, and S56A mutations affected the interaction between Bsar1p and GAP. P54A mutant protein did not interact with two regulating proteins, GEF and GAP, even though the mutation didn't affect the intrinsic GTP-binding, nucleotide exchange or GTPase activity of Bsar1p.