• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.225-225
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• 2023

하상 측정은 하천 유지관리, 수공구조물 설계 및 보수, 수생태 조사의 필수적인 자료이다. 최근 4대강 대규모 사업 이후 자연적 안정화로 인해 침식 및 재퇴적이 진행되어 정밀 하상 모니터링이 요구되고 있다. 통상적인 하상 조사 기법은 레벨측량 및 RTK-GPS 등을 활용하여 점단위로 직접 계측하는 기법과 수심이 깊을 경우 ADCP와 같은 음향측심기법을 통해 하상변동을 계측하고 있다. 하지만 점단위 직접 측정은 사구와 사련과 같은 하상 구조 교란 및 계측 시 위험을 동반하고 수심자료의 측정오차가 크게 발생하는 한계점이 존재한다. 또한 초음파 방식의 경우 막음길이와 바닥면 노이즈 등의 한계점으로 50 cm 미만의 저수심부 하상 측정이 불가능한 실정이다. 이러한 한계점을 극복하기 위해 최근 드론의 보급으로 수심라이다(Bathymetry LiDAR), SFM, 드론 탑재 초분광 영상을 활용한 초분광수심법과 같은 저고도, 고해상도의 비접촉식 면단위 하상 측정 기법이 대안으로 각광받고 있고 최근 관심은 해당 최신 기술의 성능 점검 및 적용성 평가에 있다. 따라서 본 연구에서는 초분광수심법 중 보편적으로 적용할 수 있는 최적밴드비분석(OBRA)의 성능 점검과 실무 적용성을 국내 하천을 대상으로 검토하였다. 해당 기술의 실무 적용성 평가항목 중 수심 적용 범위가 경제적이고 효율적인 성능 평가의 주된 항목이다. 선행 연구에 따르면 감천을 대상으로 저수심부의 성능 평가를 진행한 결과 상세한 하상계측이 가능하다고 제시하였다. 따라서 본 연구는 낙동강-황강 합류부를 대상으로 전형적인 평수기 탁도 조건에서 초분광수심법을 적용할 경우 최대측정가능수심의 범위를 결정하는 방법 및 결과를 제시하려고 한다. 또한 현장실험 당시 합천댐 방류로 인하여 황강의 탁도가 높아진 상태에 기인하여 고탁도 조건에서 초분광수심법의 적용성 평가도 추가 검토하였다. 해당 연구는 수심과 밴드비의 비선형성을 통해 최적 밴드비 분석의 결과로 도출될 수 있는 상관계수와 평균 제곱근 오차(RMSE)의 동향을 보아 다양한 시나리오의 배제수심을 통해 최대측정가능수심을 산정하였으며 그 이상의 범위는 수심맵 산정에서 제외하였다. 그 결과로 낙동강 본류에서 2.5 m 이하, 황강 지류에서 1.25 m 이하의 최대측정가능수심이 나타났고 해당 범위 이하에서는 상세한 하상이 나타났다. 또한 고탁도 조건인 황강에서는 낙동강에 비해 절반 수준의 최대측정가능수심 범위가 나타나 탁도 조건에 따른 초분광수심법의 한계가 있는 것을 확인하였다.

• Animal Bioscience
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• v.36 no.7
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• pp.1022-1033
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• 2023

Objective: p66Shc, a 66 kDa protein isoform encoded by the proto-oncogene SHC, is an essential intracellular redox homeostasis regulatory enzyme that is involved in the regulation of cellular oxidative stress, apoptosis induction and the occurrence of multiple age-related diseases. This study investigated the expression profile and functional characteristics of p66Shc during preimplantation embryo development in sheep. Methods: The expression pattern of p66Shc during preimplantation embryo development in sheep at the mRNA and protein levels were studied by quantitative real-time polymerase chain reaction (RT-qPCR) and immunofluorescence staining. The effect of p66Shc knockdown on the developmental potential were evaluated by cleavage rate, morula rate and blastocyst rate. The effect of p66Shc deficiency on reactive oxygen species (ROS) production, DNA oxidative damage and the expression of antioxidant enzymes (e.g., catalase and manganese superoxide dismutase [MnSOD]) were also investigated by immunofluorescence staining. Results: Our results showed that p66Shc mRNA and protein were expressed in all stages of sheep early embryos and that p66Shc mRNA was significantly downregulated in the 4-to 8-cell stage (p<0.05) and significantly upregulated in the morula and blastocyst stages after embryonic genome activation (EGA) (p<0.05). Immunofluorescence staining showed that the p66Shc protein was mainly located in the peripheral region of the blastomere cytoplasm at different stages of preimplantation embryonic development. Notably, serine (Ser36)-phosphorylated p66Shc localized only in the cytoplasm during the 2- to 8-cell stage prior to EGA, while phosphorylated (Ser36) p66Shc localized not only in the cytoplasm but also predominantly in the nucleus after EGA. RNAi-mediated silencing of p66Shc via microinjection of p66Shc siRNA into sheep zygotes resulted in significant decreases in p66Shc mRNA and protein levels (p<0.05). Knockdown of p66Shc resulted in significant declines in the levels of intracellular ROS (p<0.05) and the DNA damage marker 8-hydroxy2'-deoxyguanosine (p<0.05), markedly increased MnSOD levels (p<0.05) and resulted in a tendency to develop to the morula stage. Conclusion: These results indicate that p66Shc is involved in the metabolic regulation of ROS production and DNA oxidative damage during sheep early embryonic development.

• Animal Bioscience
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• v.36 no.7
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• pp.991-1002
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• 2023

Objective: This study aimed to elucidate the underlying gene regions responsible for productive, phenotypic or adaptive traits in different ecological types of Tibetan sheep and the discovery of important genes encoding valuable traits. Methods: We used whole-genome resequencing to explore the genetic relationships, phylogenetic tree, and population genetic structure analysis. In addition, we identified 28 representative Tibetan sheep single-nucleotide polymorphisms (SNPs) and genomic selective sweep regions with different traits in Tibetan sheep by fixation index (Fst) and the nucleotide diversity (θπ) ratio. Results: The genetic relationships analysis showed that each breed partitioned into its own clades and had close genetic relationships. We also identified many potential breed-specific selective sweep regions, including genes associated with hypoxic adaptability (MTOR, TRHDE, PDK1, PTPN9, TMTC2, SOX9, EPAS1, PDGFD, SOCS3, TGFBR3), coat color (MITF, MC1R, ERCC2, TCF25, ITCH, TYR, RALY, KIT), wool traits (COL4A2, ERC2, NOTCH2, ROCK1, FGF5, SOX9), and horn phenotypes (RXFP2). In particular, a horn-related gene, RXFP2, showed the four most significantly associated SNP loci (g. 29481646 A>G, g. 29469024 T>C, g. 29462010 C>T, g. 29461968 C>T) and haplotypes. Conclusion: This finding demonstrates the potential for genetic markers in future molecular breeding programs to improve selection for horn phenotypes. The results will facilitate the understanding of the genetic basis of production and adaptive unique traits in Chinese indigenous Tibetan sheep taxa and offer a reference for the molecular breeding of Tibetan sheep.

• Korean Journal of Environment and Ecology
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• v.15 no.3
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• pp.230-236
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• 2001

The fluxes of wet deposition(WD), throughfall(TF), stemflow(SF) and soil leachates were measured to understand base cation budgets on deciduous ecosystem impacted by acidic deposition in the north-western part of Tomakomai in Hokkaido, Japan. The flux of $H^{+}$ for wet deposition was $0.34kmo1_{c}$ $ha^{2+}$ and the flux of base cation, $K^{+}$ /, Na$^{ + }$, $Ca^{2+}$ and $Mg^{2+}$ far throughfall plus stemflow wart 1.6 kmolc $ha^{-1}$ , 3 times higher level than that for wet deposition. The flux of base cation for canopy leaching(LI) was 0.95 kmolc ha$^{-1}$ , 2.8 times higher level than $H^{+}$ sources in wet deposition. The major mechanism of $^{+}$ consumption closely related to acidic neutralizing capacity of canopy. The ionic flux for soil leachates from Boil reservoir and proton consumption in soil was dependent on soil chemical states and exchangeable Ca in soil had a major factor of H$^{+ }$ consumption. The base cation budgets on deciduous ecosystem showed positive balance fur Na, Ca and Mg, while K was the negative value.

• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.425-433
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• 2023

In this study, we developed a virtual artist play lesson model using gamification concepts and AI-generated art programs to foster creative thinking in freshman art majors. Targeting first-year students in the Digital Media Art Department at Sichuan Film & Television University in China, this course aims to alleviate fear of artistic creation and enhance problem-solving abilities. The educational model consists of four stages: persona creation, creative writing, text visualization, and virtual exhibitions. Through persona creation, students established their artist identities, and by introducing game-like elements into writing experiences, they discovered their latent creativity. Using AI-generated art programs for text visualization, students gained confidence in their creations, and in the virtual exhibitions, they were able to enhance their self-esteem as artists by appreciating and evaluating each other's works. This educational model offers a new approach to promoting creative thinking and problem-solving skills while increasing learner engagement and interest. Based on these research findings, we expect that by developing and implementing educational strategies that cultivate creative thinking, more students will grow their artistic capacities and creativity, benefiting not only art majors but also students from various fields.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.5B
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• pp.419-427
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• 2009

In existing studies about benefit of water quality improvement using WTP, the object of survey is described pre-policy water quality as "current water quality" and improved (post-policy) water quality as "boatable, fishable and swimmable". Multiply WTP by the number of households of basin is total benefit. The existing studies are not benefit of a specific water resource business but benefit of a policy on unsubstantial water resource business. Because of a lack of objectivity and oversimplification, it is difficult for survey respondents to understand an object of survey. The purpose of this study is to evaluate a benefit estimation methodology for raw water quality improvement in water resource development business. After conducting a survey of WTP of 1,000 housewives who is using water service in the National Capital region, the relational expression of water quality improvement (BOD) and WTP is derived by using statistical analysis of the survey. As a case study, the stream water quality improvement benefit of Song Li Won multipurpose dam was evaluated, which is planned to be built at the local secondary stream section on Nae Sung stream in Nak-Dong River system. As a result of study, annual average benefit evaluation is 5,980 million won on the average annual planned discharge, 8,663 million won on the planned discharge during the period except for wet season (July to October), 11,905 million won on the planned discharge during water quality declining period and 14,502 million won on the planned discharge during water quality declining period respectively. By using the relational expression of BOD-WTP, it is easy to estimate the benefit without regard for water quality.

• The Journal of Internal Korean Medicine
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• v.44 no.5
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• pp.1071-1082
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• 2023

Objective: The purpose of this study is to report the efficacy of using Korean medicine treatment with cold hypersensitivity of the hands and feet with lumbar spinal herniated intervertebral disc. Case presentation: We used Korean medicine treatment (Gyejigabuja-tang, acupuncture) to treat a hospitalized patient with cold hypersensitivity of the hands and feet with lumbar spinal herniated intervertebral disc. To evaluate the treatment, we used the Numerical Rating Scale (NRS). The degree of cold hypersensitivity of the hands and feet was also evaluated using evaluation paper. The generic health status was measured using the European Quality of Life-5 Dimensions (EQ-5D) scale. The 31-year-old female underwent 13 days of inpatient treatment. Her pattern identification was that both hands and feet are cold (BiYangHeo) types. The treatments were herbal medicine and acupuncture. She took Gyejigabuja-tang for 13 days. She also received acupuncture for 20 minutes twice a day at LI11 (曲池), L14 (合谷), TE5 (外關) on her hands, LR3 (太衝), SP6 (三陰交), SP9 (陰陵泉) ST36 (足三里), GB41 (足臨泣) on Feet, GV4 (命門), GV3 (腰陽關), BL23 (腎兪), BL24 (氣海兪), BL25 (大腸兪), and BL26 (關元兪) on the low back. After 2 weeks of treatment, the NRS scale of her cold hypersensitivity of hands and feet symptoms dropped from NRS 7 to NRS 3, and her low back pain dropped from NRS 6 to NRS 3. Her cold hypersensitivity of hands and feet evaluation paper score decreased from 64 to 32, and her EQ-5D increased from 0.487 to 0.681. After the end of treatment, continued symptom improvements and no significant side effects were confirmed. Results: These findings suggest that Korean medical treatment (Gyejigabuja-tang, acupuncture) may be effective for treating cold hypersensitivity of hands and feet with lumbar spinal herniated intervertebral disc. The NRS, evaluation paper, and EQ-5D scores improved after treatment, with no side effects.

• Animal Bioscience
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• v.37 no.4
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• pp.709-717
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• 2024

Objective: Abnormally increased somatic cell counts (SCCs) in milk is usually a sign of bovine subclinical mastitis. Mutual interaction between the host and its associated microbiota plays an important role in developing such diseases. The main objective of this study was to explore the difference between cows with elevated SCCs and healthy cattle from the perspective of host-microbe interplay. Methods: A total of 31 milk samples and 23 bovine peripheral blood samples were collected from Holstein dairy cattle to conduct an integrated analysis of transcriptomic and metagenomics. Results: The results showed that Ralstonia and Sphingomonas were enriched in cows with subclinical mastitis. The relative abundance of the two bacteria was positively correlated with the expression level of bovine transcobalamin 1 and uridine phosphorylase 1 encoding gene. Moreover, functional analysis revealed a distinct alternation in some important microbial biological processes. Conclusion: These results reveal the relative abundance of Ralstonia and Sphingomonas other than common mastitis-causing pathogens varied from healthy cows to those with subclinical mastitis and might be associated with elevated SCCs. Potential association was observed between bovine milk microbiota composition and the transcriptional pattern of some genes, thus providing new insights to understand homeostasis of bovine udder.

• Animal Bioscience
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• v.37 no.4
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• pp.609-621
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• 2024

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

• Korean Journal of Radiology
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• v.22 no.3
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• pp.366-375
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• 2021

Objective: To evaluate the radiological tumor response patterns and compare the response assessments based on immune-based therapeutics Response Evaluation Criteria in Solid Tumors (iRECIST) and RECIST 1.1 in metastatic clear-cell renal cell carcinoma (mccRCC) patients treated with programmed cell death-1 (PD-1) inhibitors. Materials and Methods: All mccRCC patients treated with PD-1 inhibitors at Henan Cancer Hospital, China, between January 2018 and April 2019, were retrospectively studied. A total of 30 mccRCC patients (20 males and 10 females; mean age, 55.6 years; age range, 37-79 years) were analyzed. The target lesions were quantified on consecutive CT scans during therapy using iRECIST and RECIST 1.1. The tumor growth rate was calculated before and after therapy initiation. The response patterns were analyzed, and the differences in tumor response assessments of the two criteria were compared. The intra- and inter-observer variabilities of iRECIST and RECIST 1.1 were also analyzed. Results: The objective response rate throughout therapy was 50% (95% confidence interval [CI]: 32.1-67.9) based on iRECIST and 30% (95% CI: 13.6-46.4) based on RECIST 1.1. The time-to-progression (TTP) based on iRECIST was longer than that based on RECIST 1.1 (median TTP: not reached vs. 170 days, p = 0.04). iRECIST and RECIST 1.1 were discordant in 8 cases, which were evaluated as immune-unconfirmed PD based on iRECIST and PD based on RECIST 1.1. Six patients (20%, 6/30) had pseudoprogression based on iRECIST, of which four demonstrated early pseudoprogression and two had delayed pseudoprogression. Significant differences in the tumor response assessments based on the two criteria were observed (p < 0.001). No patients demonstrated hyperprogression during the study period. Conclusion: Our study confirmed that the iRECIST criteria are more capable of capturing immune-related atypical responses during immunotherapy, whereas conventional RECIST 1.1 may underestimate the benefit of PD-1 inhibitors. Pseudoprogression is not rare in mccRCC patients during PD-1 inhibitor therapy, and it may last for more than the recommended maximum of 8 weeks, indicating a limitation of the current strategy for immune response monitoring.