The polymorphisms of several genes including Leptin (LEP), beta-lactoglobulin (LGB) and Prolactin receptor (PRLR) have been shown to affect milk composition traits in dairy cattle. But, the effects of these polymorphisms on the milk traits of Philippine water buffalo are still unclear. In the Philippines, buffalo are the major milk producers most of which are the Philippine carabao (PC), the American Murrah Buffalo (AMB) and Bulgarian Murrah Buffalo (BMB). The LEP, LGB and PRLR genes are considered to be associated with milk production traits. The objective of the present study was to identify the single nucleotide polymorphisms (SNPs) in the LEP, LGB and PRLR genes of PC, AMB and BMB and to investigate the effect of the SNPs on milk production traits in these buffalo. Genetic polymorphisms were screened by DNA sequencing and 12 SNPs were detected in BMB; 5 SNPs were in LEP exon3 region (G14227A, G14343A, T14502C, C14526T, G14603A); 5 SNPs were in LGB exon 2 region (G1861C, A1900G, G1901T, T1948C, G1949A); 2 SNPs were in PRLR exon 6 (T59047C, T59109C). Also, 12 polymorphism sites between cattle and buffalo were identified. Our analysis of the association between SNPs and milk production traits should be useful in future studies of buffalo breeding to improve lactation performance.
Cunde, Xiao;Dahe, Qin;Zhongqin, Li;Jiawen, Ren;Allison, Ian
Ocean and Polar Research
/
v.24
no.3
/
pp.227-236
/
2002
For ten firn cores, from both the eastern and the western side of Lambert Glacier basin (LGB), snow accumulation rate and isotopic temperature were measured far the recent 50 years. Results show that snow accumulation for five cores over the eastern side of LGB (GC30, GD03, GD15, DT001, and DT085) at Wilks Land and Princess Elizabeth Land increases, whereas it decreases at the western side (Core E, DML05, W200, LGB 16, and MGA) at Dronning Maud Land, Mizuho Plateau and Kamp Land. For the past decades, the increasing rate was $0.34-2.36kg\;m^{-2}a^{-1}$ at the eastern side and the decreasing rate was $-0.01\;-\;-2.36kg\;m^{-2}\;a^{-1}$ at the western side. Temperatures at the eastern LGB were also increased with the rate of $0.02%o\;a^{-l}$. At the western LGB it was difficult to see clear trends, which were confirmed by Instrumental temperature records at coastal stations. Although statistic analysis and modeling results display that both surface temperature and accumulation rate has increased trends in Antarctic ice sheet during 1950-2000, the regional distributions were much more different for different geographic areas. We believe that ice-core records at Wilks Land and Princess Elizabeth Land reflect the real variations of SST and moisture change in the southern India Ocean. For the Kamp Land and Dronning Maud Land, however circulation pattern was different, by which the climate was more complicated. The International Trans-Antarctic Scientific Expedition (ITASE) aimed to reveal an overall spatial pattern of climate change over Antarctic ice sheet for the past 200 years. This study points the importance of continental to regional circulation to annual-decadal scale climate change in Antarctica.
Sensor localization is one of the fundamental problems in wireless sensor networks. Previous localization algorithms can be classified into two categories, the GGB (Global Geometry-Based) approaches and the LGB (Local Geometry-Based). In the GGB approaches, there are a fixed set of reference nodes of which the coordinates are pre-determined. Other nodes determine their positions based on the distances from the fixed reference nodes. In the LGB approaches, meanwhile, the reference node set is not fixed, but grows up dynamically. Most GGB algorithms assume that the nodes are deployed in a convex shape area. They fail if either nodes are in a concave shape area or there are obstacles that block the communications between nodes. Meanwhile, the LGB approach is vulnerable to the errors in the distance estimations. In this paper, we propose new localization algorithms to cope with those two limits. The key technique employed in our algorithms is to determine, in a fully distributed fashion, if a node is in the line-of-sight from another. Based on the technique, we present two localization algorithms, one for anchor-based, another for anchor-free localization, and compare them with the previous algorithms.
Zepeda-Batista, Jose Luis;Saavedra-Jimenez, Luis Antonio;Ruiz-Flores, Agustin;Nunez-Dominguez, Rafael;Ramirez-Valverde, Rodolfo
Asian-Australasian Journal of Animal Sciences
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v.30
no.12
/
pp.1684-1688
/
2017
Objective: From a review of published information on genetic association studies, a meta-analysis was conducted to determine the influence of the genes ${\kappa}-casein$ (CSN3) and ${\beta}-lactoglobulin$ (LGB) on milk yield traits in Holstein, Jersey, Brown Swiss, and Fleckvieh. Methods: The GLIMMIX procedure was used to analyze milk production and percentage of protein and fat in milk. Models included the main effects and all their possible two-way interactions; not estimable effects and non-significant (p>0.05) two-way interactions were dropped from the models. The three traits analyzed used Poisson distribution and a log link function and were determined with the Interactive Data Analysis of SAS software. Least square means and multiple mean comparisons were obtained and performed for significant main effects and their interactions (p<0.0255). Results: Interaction of breed by gene showed that Holstein and Fleckvieh were the breeds on which CSN3 ($6.01%{\pm}0.19%$ and $5.98%{\pm}0.22%$), and LGB ($6.02%{\pm}0.19%$ and $5.70%{\pm}0.22%$) have the greatest influence. Interaction of breed by genotype nested in the analyzed gene indicated that Holstein and Jersey showed greater influence of the CSN3 AA genotype, $6.04%{\pm}0.22%$ and $5.59%{\pm}0.31%$ than the other genotypes, while LGB AA genotype had the largest influence on the traits analyzed, $6.05%{\pm}0.20%$ and $5.60%{\pm}0.19%$, respectively. Furthermore, interaction of type of statistical model by genotype nested in the analyzed gene indicated that CSN3 and LGB genes had similar behavior, maintaining a difference of more than 7% across analyzed genotypes. These results could indicate that both Holstein and Jersey have had lower substitution allele effect in selection programs that include CSN3 and LGB genes than Brown Swiss and Fleckvieh. Conclusion: Breed determined which genotypes had the greatest association with analyzed traits. The mixed model based in Bayesian or Ridge Regression was the best alternative to analyze CSN3 and LGB gene effects on milk yield and protein and fat percentages.
This study examines the moderation effect of social support and acceptance of sexual orientation on the mediation effect of through rumination on the relationship between microaggression against sexual orientation and LGB's depression. To this end, an online survey was conducted among cisgender homosexuals and bisexuals aged 18 years and older, and 380 participants were ultimately selected for the study. The results of the study were as follows. First, the correlations of all variables were significant except for the relationship between rumination and acceptance of sexual orientation. Second, rumination partially mediated the pathway of microagression against sexual orientation predicting LGB's depression. Third, the moderated mediation effect of social support and acceptance of sexual orientation on the relationship between microaggression, rumination, and depression was found. The effect size of social support was found to be larger than that of acceptance of sexual orientation. Based on these findings, the implications of the study, possible interventions in counseling, and suggestions for further research were discussed.
While it could become an alternative water resource, fog could undermine traffic safety and operational performance of infrastructures. To reduce such adverse impacts, it is necessary to have spatially continuous fog risk information. In this work, tree-based machine-learning models were developed in order to quantify fog risks with routine meteorological observations alone. The Extreme Gradient Boosting (XGB), Light Gradient Boosting (LGB), and Random Forests (RF) were chosen for the regional fog models using operational weather and visibility observations within the Jeollabuk-do province. Results showed that RF seemed to show the most robust performance to categorize between fog and non-fog situations during the training and evaluation period of 2017-2019. While the LGB performed better than in predicting fog occurrences than the others, its false alarm ratio was the highest (0.695) among the three models. The predictability of the three models considerably declined when applying them for an independent period of 2020, potentially due to the distinctively enhanced air quality in the year under the global lockdown. Nonetheless, even in 2020, the three models were all able to produce fog risk information consistent with the spatial variation of observed fog occurrences. This work suggests that the tree-based machine learning models could be used as tools to find locations with relatively high fog risks.
Pyo, Sohwi;Lee, Seul Ah;Jang, Aekyung;Lee, Joonwoo;Lee, Eunbi;Yang, Eunjoo
Korean Journal of Culture and Social Issue
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v.28
no.2
/
pp.241-272
/
2022
This purpose of this study was to use the meta-summary method to systematically integrate the findings of the heterogeneous qualitative research on the factors influencing the sexual identity formation process of lesbian, gay, and bisexual groups in Korea. The integrative investigation of the universal or unique factors affecting the identity formation of sexual minority is important to understand their internal experiences and support their psychological adaptation. Using a qualitative meta-summary, this study systematically identified and abbriviated the psychological and social factors that promote or impede the sexual identity acceptance and integration of LGB individuals. First, a total of 9 studies which were published in Korea up to 2021 were identified through the research database. The included studies were critically evaluated by using the Critical Appraisal Screening Programme tool. A total of 179 statements were extracted from the findings of each study and 34 abstracted statements were generated. The 34 abstracted statements were categorized into four groups, psychological factors that promote or impede sexual identity formation and social factors that promote or impede it. These four main categories and corresponding factors in each category were explained. The implications and limitations were also discussed.
Journal of the Korea Society of Computer and Information
/
v.18
no.12
/
pp.55-64
/
2013
In this paper, the HILS (Hardware In-the-Loop Simulation) system to analyze and to verify the performance of the targeting pod is addressed. The main functions of the targeting pod is acquiring and tracking targets to guide a LGB (Laser Guided Bomb) to the targets. For the analysis of targeting pod, the real time simulate images generation of IR and daylight cameras, sever control technology, and the analysis of laser transfer characteristics are necessary. For the real time image generation and the laser transfer characteristics analysis, off-the-shelf SDK(Software Development Kit) OKTAL-SE is used. For the servo controller, well-proven mechanism in the previous program is applied to increase servo control accuracy. To analyze the performance of a targeting pod in a realistic environment, 1553B, ARINK818 interface and etc. which are actually implemented in real combat aircrafts are applied in the system. By using the developed HILS system, the performance of currently operating targeting pods in real combat aircrafts can be analyzed and predicted. Additionally, the relationship between overall system performance and each module performance can be analyzed, the currently developed HILS system is expected to be a very useful tool to generate system development requirements of targeting pods and to reduce any possible future development risks.
Objective: The aim of the study was to find a possible association between the ${\beta}-$ and ${\kappa}-casein$ and ${\beta}-lactoglobulin$ genotypes and important milk physiochemical and technological characteristics such as acidity, alcohol stability, the contents of some minerals and the parameters of acid fermentation ability (FEA) in Czech Fleckvieh Cattle. Methods: Milk and blood samples were collected from 338 primiparous Czech Fleckvieh cows at the same stage of lactation. The genotypes of individual cows for ${\kappa}-casein$ (alleles A, B, and E) and ${\beta}-lactoglobulin$ (alleles A and B) were ascertained by polymerase chain reaction-restriction fragment length polymorphism, while their ${\beta}-casein$ (alleles $A^1$, $A^2$, $A^3$, and B) genotype was determined using melting curve genotyping analysis. The data collected were i) milk traits including active acidity (pH), titratable acidity (TA), alcohol stability (AS); calcium (Ca), phosphorus (P), sodium (Na), magnesium (Mg), and potassium (K) contents; and ii) yoghurt traits including active acidity (Y-pH), titratable acidity (Y-TA), and the counts of both Lactobacilli and Streptococci in 1 mL of yoghurt. A linear model was assumed with fixed effects of herd, year, and season of calving, an effect of the age of the cow at first calving and effects of the casein and lactoglobulin genotypes of ${\beta}-CN$ (${\beta}-casein$, CSN2), ${\kappa}-CN$ (${\kappa}-casein$, CSN3), and ${\beta}-LG$ (${\beta}-lactoglobulin$, LGB), or the three-way interaction between those genes. Results: The genetic polymorphisms were related to the milk TA, AS, content of P and Ca, Y-pH and Lactobacilli number in the fresh yoghurt. The CSN3 genotype was significantly associated with milk AS (p<0.05). The effect of the composite CSN2-CSN3-LGB genotype on the investigated traits mostly reflected the effects of the individual genes. It significantly influenced TA (p<0.01), Y-pH (p<0.05) and the log of the Lactobacilli count (p<0.05). Conclusion: Our findings indicate that the yoghurt fermentation test together with milk proteins genotyping could contribute to milk quality control and highlight new perspectives in dairy cattle selection.
Objective: The aim of the study was to evaluate the influence of gene polymorphisms and nongenetic factors on the somatic cell score (SCS) in the milk of Holstein (n = 148) and Simmental (n = 73) cows and their crosses (n = 6). Methods: The SCS was calculated by the formula SCS = log2(SCC/100,000)+3, where SCC is the somatic cell count. Polymorphisms in the casein alpha S1 (CSN1S1), beta-casein (CSN2), kappa-casein (CSN3), beta-lactoglobulin (LGB), acyl-CoA diacylglycerol transferase 1 (DGAT1), leptin (LEP), fatty acid synthase (FASN), stearoyl CoA desaturase 1 (SCD1), and 1-acylglycerol-3-phosphate O-acyltransferase 6 (AGPAT6) genes were genotyped, and association analysis to the SCS in the cow's milk was performed. Further, the impact of breed, farm, year, month of the year, lactation stage and parity on the SCS were analysed. Phenotype correlations among SCS and milk constituents were computed by Pearson correlation coefficients. Results: Only CSN2 genotypes A1/A2 were found to have significant association with the SCS (p<0.05), and alleles of CSN1S1 and DGAT1 genes (p<0.05). Other polymorphisms were not found to be significant. SCS had significant association with the combined effect of farm and year, lactation stage and month of the year. Lactation parity and breed had not significant association with SCS. The phenotypic correlation of SCS to lactose content was negative and significant, while the correlation to protein content was positive and significant. The correlations of SCS to fat, casein, nonfat solids, urea, citric acid, acetone and ketones contents were very low and not significant. Conclusion: Only CSN2 genotypes, CSN1S1 and DGAT1 alleles did show an obvious association to the SCS. The results confirmed the importance of general quality management of farms on the microbial milk quality, and effects of lactation stage and month of the year. The lactose content in milk reflects the health status of the udder.
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