• 제목/요약/키워드: LEE Myong-ki

검색결과 252건 처리시간 0.024초

Purification and Characterization of Cold Active Lipase from Psychrotrophic Aeromonas sp. LPB 4

  • Lee, Han-Ki;Ahn, Min-Jung;Kwak, Sung-Ho;Song, Won-Ho;Jeong, Byeong-Chul
    • Journal of Microbiology
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    • 제41권1호
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    • pp.22-27
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    • 2003
  • A lipase from Aeromonas sp. LPB 4, a psychrotophile isolated from a sea sediment was purified and characterized. The lipase was purified 53.5 fold to a homogeneous state by acetone precipitation and QAE sephadex column chromatography and its molecular weight was determined to be 50 kDa by SDS-PAGE. The enzyme exhibited maximum activity at 10$^{\circ}C$ and was stable at temperatures lower than 50$^{\circ}C$. This lipase favored substrates containing medium carbon chain of acyl group, while too low and high carbon chain decreased its activity. The lipolytic activity of purified lipase was slightly increased by the addition of 0.1% detergent, but decreased by 1% of detergent. Butanol severely decreased the lipase activity while methanol increased the activity about 15%.

Global Mobile Ad Hoc Network에서 다중경로를 이용한 네트워크 성능향상에 관한 연구 (A Study on Network Performance Improvement Using Multipath in Global Mobile Ad Hoc Network)

  • 김재호;배진승;정찬혁;이기원;문태수;하재승;유충렬;김현욱;이광배
    • 전기전자학회논문지
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    • 제12권1호
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    • pp.18-26
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    • 2008
  • 유비쿼터스 환경의 출현에 따라 멀티 홉 통신이 탑재된 이동 단말기로 쉽게 유선 인터넷망에 접속하여 시스템을 제어하거나 정보를 공유하는 유.무선 통합망 연구가 활발히 진행 중이다. 현재 이동 Ad Hoc 네트워크와 유선망을 연결한 기존 유 무선 통합망에서는 게이트웨이를 이용한 이기종 네트워크 인터페이스 설정과 관련된 연구만 이루어지고 있는 실정이다. 제안한 알고리즘은 경로 상에서 에러 발생 시 데이터 손실을 막기 위해 송신단말기와 목적지 단말기사이에 독립적인 다중경로를 설정하였다. 그 결과, 경로 에러 시 지속적으로 데이터를 전송하게 함으로써 네트워크의 신뢰성을 향상시킬 수 있었다.

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키토산과 알긴을 이용한 생분해성 필름의 제조와 생체분해 (Preparation and Biodegradation of Biodegradable Film Using Chitosan and Algin)

  • 오세영;윤철훈;이기창;박종주;황성희
    • 한국응용과학기술학회지
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    • 제16권1호
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    • pp.67-73
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    • 1999
  • Chitosan and Algin are known as a natural polymers. Biodegradable films were prepared by solution blend method in the weight ratio of natural polymer(low, medium, high-Chitosan, Algin) for the purpose of useful bioimplants. The possibility of bioimplants, which were prepared from natural polymers as a skin substitute and food wrapping materials were evaluated by measuring biodegradability. This biodegradable films were inserted in the back of rats and their biodegradability was investigated by hematological change evaluation as a function of time to biotransformation. It was found that these values of biodegradable films give some good results with short period test.

차동전류 보호계전을 위한 변압기 특성분석 (Analyses of Transformer Characteristics for Current Differential Protective Relaying)

  • 김명철;강상희;이승재;김기화;김일동
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1996년도 하계학술대회 논문집 B
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    • pp.671-673
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    • 1996
  • Simulation methods using EMTP to analyze power transformer transient characteristics and their interesting results are presented in this paper. Because of the transformer saturation including initial inrush, the conventional current differential relay with harmonic restraint module does not provide a clear distinction between internal faults and other conditions. Providing the bases to develop a new relaying concept for power transformer protection, transformer nonlinear transient characteristics are analyzed.

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A Study on the Library Development for Power Electronics Circuits Analysis

  • Seo, Young-Soo;Hwang, Lak-Hoon;Cho, Moon-Taek;Ho bin Song;Lee, Chun-Sang;Sang-Yong;Park, Ki-Soo
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2000년도 ITC-CSCC -2
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    • pp.997-1000
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    • 2000
  • The purpose of this paper is to verify the appropriation of power electronics circuit by applying the most powerful and widely used simulator PSPICE and SIMULINK for adapted variable control technics. Power electronics librarys modeled and adapted to circuit. It is proved that simulation and excute are almost same.

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혼합세제에 관한 연구 (A Study on the Mixture of Detergents)

  • 최광훈;권순자;이기창;황용현
    • 한국응용과학기술학회지
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    • 제4권1호
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    • pp.73-75
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    • 1987
  • Linear alkylbenzene sulfonate (LAS) from the mixture of anionic and nonionic detergents (containing amide type and alkylphenoxy polyethanol type) is identified by Fourier transform infrared spectrometer, separated using physical properties (solubility) and determined by nuclear magnetic resonance spectroscopy.

보호계전기의 정정 협조 전문가 시스템 (AN EXPERT SYSTEM FOR SETTING AND COORDINATION OF PROTECTIVE RELAYS)

  • 이승재;김기화;조용익;윤상현;윤만철;이상옥
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1991년도 하계학술대회 논문집
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    • pp.358-362
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    • 1991
  • This paper describes enhancements made on PROSET, an, expert system which can perform setting and coordination of protective relays used in the ultra-high voltage transmission systems. Enhancements include the friendly and convenient environment for rulebase management and system manipulation, expanded setting capability, and faster processing speed which have been achieved through adoption of the new rule representation, rule order independent IE, rulebase editor, local database generator, Interface to PSS/E fault program, RB expansion etc.

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1,1,1-TCE에 대한 비수계성 대체세정제의 최적 세정공정 (The Optimum Cleaning Process of Non-aqueous Alternative Solvents for 1,1,1-TCE)

  • 정덕채;이기창;공승대;목갑영;이석우
    • 한국응용과학기술학회지
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    • 제16권3호
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    • pp.237-240
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    • 1999
  • This study showed that the optimized cleaning process using non-aqueous cleaning solvents is adaptable in the industrial field for existing 1.1.1-TCE cleaning solvents which is an ozone depleting sustance. Alternative cleaning solvent system substituted for existing cleaning solvent against non-aqueous pollutants(cutting & flux oil), was evaluated for the cleaning efficiency using gravimetric analysis method and surface change of sample by Image analyzer. The results showed that alternative solvents and process had excellent cleaning efficiency.

국내산 천연점토를 이용한 Pb(II)의 제거효과 (Removal Effects of Pb(II) using Natural Clays from Domestic)

  • 오세영;황성규;윤철훈;이기창;장관식
    • 한국응용과학기술학회지
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    • 제15권3호
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    • pp.13-19
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    • 1998
  • This study was carried out to investigate characteristics and Pb(II) removal effect of natural Muan, Seosan, Kangjin clays in the batch mode test to develope the low-priced treatment agent of wastewater which contain heavy metals in order structural, optical properties and chemical compositions of natural clays from domestic have been investigated to have high specific surface area and have minerals such as $SiO_2$ and $Al_2O_3$. As a result, removal effects of Pb(II) ions on clays were reached at equilibrium in aqueous solutions by stirring about 20minutes. The removal effect of Pb(II) ions was best for Seosan clay than Muan or Kangjin clays in terms of fixed time. The results show the possibility of continuous treatment system of wastewater which contain heavy metals by using natural clays from domestic.