• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.861-868
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• 2013

This study investigated the effects of Opuntia ficus-indica and other natural resources (OF) in db/db and C57 mice. Plasma triglycerides, cholesterol, alanine aminotransferase (ALT) activity, aspartate aminotransferase (AST) activity, fecal bile acid excretion, the histopathological appearance of the liver, and cholesterol-related mRNA expression were determined. Mice (12 db/db mice and 12 C57 mice) were assigned to diabetic-control (db-C), diabetic-OF treatment (db-OF), normal-control (C57-C), and normal-OF treatment (C57-OF) groups. Animals in the control group were fed an AIN-76 recommended diet and animals in the OF group were fed an experimental diet containing 5% of OF for 4 weeks. Concentrations of total plasma cholesterol, triglyceride, low density lipoprotein (LDL)-cholesterol, and very low density lipoprotein (VLDL)-cholesterol decreased with the administration of OF. In contrast, high density lipoprotein (HDL)-cholesterol levels were minimally affected by the experimental diet. Plasma AST and ALT showed lower activities in the db-OF group, and the fecal excretion of bile acid was reduced in the db-OF group. Histopathological analysis of the liver showed that fatty liver conditions in the db-OF group were more improved than db-C. Low-density lipoprotein receptor (LDL-R) and cholesterol 7${\alpha}$-hydroxylase (CYP7A1) mRNA expression were increased in the db-OF group as well. However, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R) mRNA expression was lower in the db-OF group. These results provide experimental evidence about improved lipid metabolism of the OF feeding in the db/db mice.

• Journal of Life Science
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• v.18 no.12
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• pp.1686-1692
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• 2008

This study was performed to investigate the effects of the chitosan oligosaccharide administration on the improvement of the lipid compositions, blood glucose and enzyme activities in the serum of hypercholesterolemic rats fed the experimental diets for 5 weeks. Concentrations of total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, triglyceride (TG), phospholipid (PL) and blood glucose in serum were higher in the hypercholesterolemic group (group CW) and cholesterol+chitosan oligosaccharide administration group (group CCW) than those in the control group (group BW, basal diet+water). But the concentrations of total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, TG, PL and blood glucose in serum were lower in the group CCW than those in the CW, whereas the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration in the group CCW were higher than in the group CW. The activities of alkaline phosphatase (ALP) and aminotransferase (AST, ALT) in serum were lower in the group CCW than in the hypercholesterolemic group CW. From the above results, it was suggested that chitosan oligosaccharide administration was effective in the prevention and improvement of the lipid level, blood glucose and enzyme activities in serum of hypercholesterolemic rats.

• Journal of Ginseng Research
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• v.10 no.1
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• pp.114-121
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• 1986

Effect of saponin fraction of ginseng C.A. Meyer on blood serum lipoprotein distribution of high cholesterol fed rabbits for two to four weeks was investigated. A. significant increase of very low density lipoprotein(VLDL) and low density lipoprotein(LDL) occured while high density lipoprotein(HDL) were decreased in the blood of both groups which were fed high cholesterol diet with (test group) and/or without ginseng saponin(control group) for 2-4 weeks. However the degrees of VLDL and LDL increase and HDL decrease were relatively small in ginseng administered group compared with control group. This suggests that the hypocholesterolemic action of the saponin might be brought about by decreasing the raised VLDL and LDL level and increasing the lowered HDL level.

• Journal of Microbiology and Biotechnology
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• v.32 no.11
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• pp.1406-1415
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• 2022

The formation of macrophage foam cells stimulated by oxidized low-density lipoprotein (ox-LDL) is deemed an important cause of atherosclerosis. Transcription factor Yin Yang 1 (YY1), which is a universally expressed multifunctional protein, is closely related to cell metabolism disorders such as lipid metabolism, sugar metabolism, and bile acid metabolism. However, whether YY1 is involved in macrophage inflammation and lipid accumulation still remains unknown. After mouse macrophage cell line RAW264.7 cells were induced by ox-LDL, YY1 and proprotein convertase subtilisin/kexin type 9 (PCSK9) expressions were found to be increased while low-density lipoprotein receptor (LDLR) expression was lowly expressed. Subsequently, through reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Western blot analysis, Oil Red O staining and cholesterol quantification, it turned out that silencing of YY1 attenuated the inflammatory response and lipid accumulation in RAW264.7 cells caused by ox-LDL. Moreover, results from the JASPAR database, chromatin immunoprecipitation (ChIP) assay, luciferase reporter assay and Western blot analysis suggested that YY1 activated PCSK9 by binding to PCSK9 promoter and modulated the expression of LDLR in the downstream of PCSK9. In addition, the results of functional experiments demonstrated that the inhibitory effects of YY1 interference on ox-LDL-mediated macrophage inflammation and lipid accumulation were reversed by PCSK9 overexpression. To sum up, YY1 depletion inhibited its activation of PCSK9, thereby reducing cellular inflammatory response, cholesterol homeostasis imbalance, and lipid accumulation caused by ox-LDL.

• Preventive Nutrition and Food Science
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• v.4 no.1
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• pp.57-61
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• 1999

It has been postulated that oxidative stress may be increased and antioxidant defenses reduced in diabetes patients. Twenty-four patients with type 1 diabetes melitus (DM) (12.8$\pm$1.7 years) and 24 nondiabetics (12.5$\pm$2.1 years) were included in this study. Serum total cholesterol and LDL -cholesterol levels were significantly higher in diabetic than in nondiabetic control subjects, but serum levels of triglyceride , retinol , tocopherol, and $\beta$-carotene were significantly lower. Both $\beta$carotene and tocopherol levels inversely correlated with HbAlc, suggesting perhaps that low serum antioxidant level enhance theglycosylation of hemoglobin. Subjects with type 1 DM had lipid peroxide levels similar to those of nondiabetics control subjects, suggesting that per-oxdation of circulating lipid is not increased in uncomplicated diabeteics. The correlation between antioxidants and serum lipids were as follows ; retinol and LDL (r--0.36, p=0.019) ; retinol and total cholesterol(r=-0.35, p=0.020), tocopherol and LDL(r=-0.47, p=0.002) ; tocopherol and cholesterol (r=-0.49, p=0.001) ; $\beta$-carotene and LDL (r=-0.51, p=0.001). Overall , the results of this study were that serum lipid peroxide in patients with type 1 DM was similar to those of control subjects and antioxidants such as retinol, tocopherol and $\beta$-carotene were lower than those of nondiabetic cotnrol subjects, and negatively correlated with serum total cholesterol and LDL-cholesterol.

• Anxiety and mood
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• v.8 no.2
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• pp.120-126
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• 2012

Objective : Previous studies reported a correlation between the low serum cholesterol level and impulsive behaviors. In this study, we investigate an association between the serum lipid levels and psychological parameters in maladaptive soldiers in the Korean Army. Methods : A total of ninety-six maladaptive subjects and thirty-six normal controls in the Korean army were evaluated with the Korean version of Barratt Impulsiveness Scale (K-BIS), Korean version of Beck Suicidal Ideation Scale (K- BSIS), Korean version of Beck Depression Inventory (K-BDI) and Korean version of Beck Anxiety Inventory (K-BAI). Serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) level were measured by overnight fasting blood sampling. Results : There were no significant differences between the groups in demographic characteristics. Serum total cholesterol levels (t=-2.209, p=0.032), triglyceride levels (t=-4.593, p<0.001), and LDL levels (t=-3.753, p=0.001) of maladaptive subjects were significantly lower than those of normal controls, and maladaptive subjects had higher K-BIS scores than normal controls (t=7.542, p<0.001). Negative correlation was found between LDL levels and non-planning impulsiveness in the maladaptive subjects (r=-0.253, p=0.013). LDL levels (${\beta}=-0.258$, p=0.008) and K-BDI scores (${\beta}=0.266$, p=0.043) emerged as significant predictors for non-planning impulsiveness. Conclusion : These results suggested that LDL level was associated with non-planning impulsiveness. These findings suggested that serum cholesterol levels might be available as a biological marker of impulsiveness. However, more large samples, longitudinal biological study and psychiatric evaluations should be needed to develop a preventive intervention for maladaptive male conscripts in the Korean army.

• Nutrition Research and Practice
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• v.1 no.1
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• pp.65-69
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• 2007

We examined the associations between adiponectin or leptin and serum ICAM-1 levels in seventy-six hypercholesterolemic patients (mean age 59 yrs, 25 males and 51 females, LDL-cholesterol>=130mg/dL at screening). Blood lipid profiles and HOMA-IR derived from fasting glucose and insulin concentrations were determined. Serum levels of adiponectin, leptin and ICAM-1 were analyzed using ELISA The results showed that serum levels of leptin were positively associated with serum levels of ICAM-1 independent of age, sex and BMI (r=0.392, p<0.001). Serum levels of adiponectin were negatively associated with serum levels of ICAM-1 independent of age, sex and BMI (r=-0.343, p<0.005). Stepwise multiple linear regression analysis showed that serum leptin was an independent factor to be associated with serum ICAM-1 levels after adjusting for age, sex, BMI, alcohol intake, smoking status, blood lipids such as total cholesterol, triglyceride, HDL cholesterol and LDL cholesterol and HOMA-IR (p<0.001). With respect to adiponectin, its association with serum ICAM-1 was attenuated but still significant when further adjustments were made for age, sex, BMI, alcohol intake, smoking status, blood lipids such as total cholesterol, triglyceride, HDL cholesterol and LDL cholesterol and HOMA-IR (p<0.005). In conclusion, this study suggests that adiponectin and leptin are associated with endothelial derived inflammation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.1
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• pp.170-179
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• 1994

Atherosclerosis, a multifactorial disease, is closely related to lipid nutrition , Data from well known epidemiological studies including Seven Country , Framinghsam Study and several intervention trials have confirmed that serum cholesterol is the major risk factor and elevation of LDL-cholesterol level is most undesriable. On the basis of results concurring in that dietary saturated fat and cholesterol increase serum cholesterol while polyunsaturated fat decrease it, changes in serum choesterol level have been predicted by regression equations developed by Keys et al. and other investigators. Effects of individual fatty acids on the level of serum cholesterol have been further differentiated by chain length, cis-trans isomers and n-6 vs n-3 polyunsaturated fatty acids. Among them the effect of n-3 fatty acids has been well recogniaed as antiplatelet activity, thus reducing the incidence of atherosclerosis. Role of vitamin E in prevention of atherosclerosis has been evovled from works showing that LDL oxdiation stimulates formation of ateroma and also from epidemiologic studies. Dietary recommendations at present are : (ⅰ) 30 and 10 cal % as upper limit of total and saturated fat intakes, respectively (ⅱ) no more than 300 mg cholesterol/day, (ⅲ) 1-2 g of n-3 fatty acid/day and (ⅳ) some increase RDA of vitamin E which is 8-10 TE.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1283-1292
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• 2008

This study was designed to investigate the hyperlipidemia-healing effect of water extract from Iksukangjiryung, it was performed on the hyperlipidemia of rats induced by Triton WR-1339 and high cholesterol diet. We prepared two types of hyperlipidemia model in rats induced by Triton WR-1339 and high cholesterol diet. The amount of total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, lipid peroxide, glutathione and the activity of superoxide dismutase(SOD) were measured. The water extract from Iksukangjiryung showed significant decreasing effects on total cholesterol, LDL- cholesterol, triglyceride, lipid peroxide in the both model. And it showed significant increasing effects on the activity of SOD in both model and amount of glutathione in Triton WR-1339 model. These results suggest that water extract from Iksukangjiryung has healing efficacy on hyperlipidemia induced by Triton WR-1339 and high cholesterol diet.