• Fisheries and Aquatic Sciences
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• v.22 no.10
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• pp.21.1-21.7
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• 2019

Background: Lactococcus garvieae is one of the most important risk factors in the rainbow trout culture. Therefore, the purpose of this study was to identify and detect strains isolated from rainbow trout suspected of having Lactococcus garvieae using biochemical characteristics and PCR and determination of the degree of severity of isolated strains. Methods: In this study, the cause of lactococcosis in selected rainbow trout farms in Kohkilooieh and Boyerahmad province was assayed. Gram-positive and catalase-negative bacterial isolates were first obtained from selected trout fish farms using conventional biochemical tests and PCR assay. The 10-day LD₅₀ method (concentration causing 50% mortality in 10 days) was used to determine the severity of the isolated bacteria. Results: One bacterial isolate was detected from all sampled fish which confirmed as Lactococcus garvieae using a specific PCR assay based on the 16S rDNA gene by producing a single band of 1107 bp. Analysis of the rate of mortality showed that the 10-day LD₅₀ was 4.6 × 10⁵ CFU/fish. The results of this study showed that isolated bacteria had high severity for rainbow trout. The presence of bacteria in internal organs of suspected fish showed a severe systemic infection in challenged fish. Antibiogram assay also indicated that the isolated Lactococcus garvieae were resistant to some mostly used antibiotics in rainbow trout. Conclusions: According to current research, it can be concluded that the condition of lactococcosis in the studied area is not suitable, and despite the presence of disease, there is no proper action to control and prevent the disease. Unfortunately, isolated bacteria from the studied area have a very high severity compared to bacteria isolated from other regions of the country or other countries. Therefore, further investigation is needed to determine the cause of this difference and possibly in the design of the vaccine.

• The Journal of Internal Korean Medicine
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• v.33 no.2
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• pp.145-159
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• 2012

Objectives : The object of this study was to obtain acute toxicity information (single oral dose toxicity) of Armeniacae Semen (AS), a medicinal herb used for treating constipation and various respiratory diseases, in rats. Methods : In order to observe the $LD_{50}$ (50% lethal dose), approximate lethal dosage (ALD) and target organs, AS aqueous extracts were orally administered once to female and male Sprague Dawley rats at dose levels of 2,000, 1,000, 500 and 0 (control) mg/kg (body weight). The mortality, changes in the body weight, clinical signs and gross observation were monitored for 14 days after single oral treatment of AS extracts, and the organ weights and histopathological findings of principle organs were observed after sacrifice. Results : After single oral treatment of AS 2,000 and 1,000 mg/kg, all (5/5; 100%) female and male rats died within 30 minutes after treatment, while no mortalities were observed in the female and male rats treated with 500 mg/kg of AS extract. Therefore, $LD_{50}$ in female and male rats was calculated as 741.95 mg/kg. Seizure, loss of locomotion, salivation, increases of respiration and heart-beat were observed after AS extract treatment, which were observed in all rats including the lowest dosage group, 500 mg/kg in the present study. In addition, lung congestion was visible in all mortal rats with AS 2,000 and 1,000 mg/kg, respectively. Conclusions : The results obtained in this study suggest that AS extract ranges in Class III, because the $LD_{50}$ and ALD in both female and male rats were calculated as 741.95 mg/kg and 500~1,000 mg/kg, respectively. However, AS extract should be carefully treated at clinical applications, because salivation, increase of respiration and heart-beat were also observed in the lowest dosage group, 500 mg/kg in the present study.

• Journal of Life Science
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• v.17 no.4 s.84
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• pp.561-567
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• 2007

The present study was carried out to investigate the acute toxicity of Leuconostoc kimchii GJ2(Leu. kimchii GJ2), an exopolysaccharide-producing lactic acid bacteria isolated from kimchi, in ICR male and female mice. The test article was intraperitoneally or orally administered to both sexes of mice. Then followed a 14-day period of observation. In intraperitoneal acute toxicity test, mice were injected intraperitoneally with dosage of 0, 700, 900, 1,100, 1,300, 1,500, 1,700, 1,900, 2,100, 2,300 and 2,500 mg/kg. Intraperitoneal 50% lethal doses($LD_{50}$) of Leu. kimchii GJ2 was >2,500 mg/kg in male and female mice. In oral acute toxicity test, male and female mice were gavaged with Leu. kimchii GJ2 of five doses(0, 625, 1,500, 2,500 and 5,000 mg/kg). Oral $LD_{50}$ of Leu. kimchii GJ2 was consider to be higher than 5,000 mg/kg. Furthermore, no significant changes in general conditions, body weights, clinical signs and any gross lesions were observed in both sexes of mice administered intraperitoneally or orally with Leu. kimchii GJ2. In conclusion, our results suggest that no adverse effect level of Leu. kimchii GJ2 is estimated to be over 2,500 mg/kg in the intraperitoneal route and 5,000 mg/kg in the oral route, respectively.

• Food Science and Preservation
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• v.19 no.2
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• pp.315-321
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• 2012

The $in$ $vivo$ single-dose acute toxicity of $Lactobacillus$ $plantarum$ AF1, a lactic acid bacterium isolated from kimchi, in ICR male and female mice was investigated. The test article was intraperitoneally or orally administered once to both sexes of mice. The motalites, clinical findings, autopsy findings, and body weight changes were monitored daily for 14 days. In the oral acute toxicity test, the male and female mice were gavaged with four doses (5.0, 2.5, 1.25 and 0.625 g/kg) of $Lb.$ $plantarum$ AF1. The oral $LD_{50}$ of the $Lb.$ $plantarum$ AF1 was considered higher than 5.0 g/kg. In the intraperitoneal acute toxicity test, mice were injected intraperitoneally with dosages of 0.7, 0.9, 1.1, 1.3, 1.5, 1.7, 1.9, 2.1, 2.3 and 2.5 g/kg. The intraperitoneal 50% lethal dose ($LD_{50}$) of the $Lb.$ $plantarum$ AF1 was >2.5 g/kg in the male and female mice. No significant changes in the general conditions, body weights, clinical signs, and gross lesions were observed in both sexes of mice to which $Lb.$ $plantarum$ AF1 was administered intraperitoneally or orally. The results suggest that the no-adverse-effect level of $Lb.$ $plantarum$ AF1 is estimated to be more than 5.0 g/kg in the oral route and 2.5 g/kg in the intraperitoneal route.

• Korean Journal of Food Science and Technology
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• v.11 no.4
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• pp.224-231
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• 1979

The safety of the sweetening component of stevia was studied by administrating it to the rats. The $LD_{50}$ determined by intraperitoneal injection was 3,400 mg/Kg as the stevia extract containing 50 % stevioside, i.e. $LD_{50}$ of stevioside was more than 1,700 mg/Kg. Oral administration of large quantities of the stevia extract for 56 days resulted in no effect on the growth of rats. The analyses of total blood (RBC, WBC, Hb and Hct), 17 blood serum components including total protein, glucose, cholesterol, GOT, and 11 items of findings on the liver tissues including nuclear deterioration of liver cells, proliferation of Kupffer cells, fibrosis of portal area showed no significant differences between control and treatments except lactate dehydrogenase activity after 56 day-oral administration of the extract. From the results obtained, it was supposed that the stevia extract/stevioside revealed no acute or sub-acute toxic effects on rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.3
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• pp.527-533
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• 2002

In the screening of Korean traditional tea sources for the cellular lysosomal enzyme activity of peritoneal macrophage from mice, CT-0, a cold-water extract from Carthamus tinctorius L., showed the highest macro-phage-stimulating activity. CT-1-IIa-2-1, a purified macrophage-stimulation polysaccharide was obtained by a series of purification steps such as anion exchage chromatography with DEAE-Toyopearl 650M, gel permeation chromatography with Sepharose CL-6B, Sephacryl S-200, and HPLC with Superdex G-75. The molecular weight of homogeneous purified polysaccharide was estimated about 68 kDa. CT-1-IIa-2-1 consisted of xylose 27.44%, arabinose 16.14%, mannose 15.92% and glucose 14.47%. To measure acute toxicity, dose of 50, 100, 500, and 1000 mg/kg were intraperitoneally injected to ICR mice. The LD$\_$50/ was about 397 mg/kg.

• Korean Journal of Acupuncture
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• v.36 no.2
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• pp.127-138
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• 2019

Objectives : This study was carried out in order to toxicity and safety of Drosera Rotundifolia L. pharmacopuncture in BALB/c mice. Methods : In order to investigate toxicity and safety of Drosera Rotundifolia L. pharmacopuncture, BALB/c mice were chosen in these studies and injected to ST36. In 50% Lethal Dose (LD 50) study, mice were divided into 8 groups. Doses of pharmacopuncture at 300, 600, 1,200, 2,400, 3,600, 4,800, 6,000, 7,200 mg/kg were given to the each group. we observed mortality and toxic signs for 7 days after injection. In single-dose toxicity, mice were divided into 4 groups. Doses of pharmacopuncture, 300, 600, 1,200 mg/kg, were administered to the experimental group. And a dose of normal saline, 0.2 ml was administered to the control group. We observed mortality and toxic signs for 14 days after injection. In repeated-dose toxicity for 4 weeks, mice were divided in 4 groups. Doses of pharmacopuncture, 150, 300, 600 mg/kg, were administered to the experimental group. And a dose of normal saline, 0.2 ml, was administered to the control group. We observed mortality, toxic signs, body weight, hematological values et al. Results : The LD 50 of Drosera Rotundifolia L. pharmacopuncture was 4,800 mg/kg. In single-dose toxicity, no deaths, no toxic signs occurred in any of groups. In repeated-dose toxicity for 4 weeks, no deaths, no toxic signs occurred in any of groups. Conclusions : No Observed Adverse Effect Level (NOAEL) of Drosera Rotundifolia L. pharmacopuncture injection for 4 weeks was more than 600 mg/kg.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.43 no.3 s.309
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• pp.60-66
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• 2006

The purpose of this study was to suggest a new detection method for early diagnosing diabetic neuropathic foot by obtaining a ratio of toe to finger blood flow using photoplethysmography(PPG) and Laser Doppler(LD). Nerve conduction velocity (NCV) has been routinely used for diagnosing neuropathic foot, but it applies strong electric stimulus to peripheries resulting in stress and pain. The blood flow ratio of 50 neuropathic diabetes($0.96{\pm}0.20$) was significantly higher than that of 64 normal person($0.46{\pm}0.15$)(p<0.000). It also showed that toe temperature of neuropathic diabetes($30.5{\pm}1.4^{\circ}C$) was significantly higher than that of normal group($29.3{\pm}2.0^{\circ}C$)(p<0.000). The optimal boundary value of the blood flow ratio was found to be 0.678 and the sensitivity and specificity of this proposed method resulted in 95.3% and 95.3% respectively. Lastly, there were no neuropathic diabetes whose temperature difference between finger and toe was higher than $4.5\;^{\circ}C$.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.117-123
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• 2006

Fumigant toxicity of 34 plant essential oils were tested against female adults of Culex pipiens pallens. Seven oils (basil, coriander, fennel, pennyroyal, peppermint, thyme red, thyme white) showed more than 60% mortality at 1 ${\mu}L\;L^{-1}$ air concentration and pennyroyal oil appeared to have the highest adulticidal activity. GC and GC-MS analysis of the seven essential oils and bioassay of their components revealed that pulegone (a major component of pennyroyal oil) and anethole (a major component of fennel and basil oil, $LD_{50}$, ${\mu}L\;L^{-1}$ air =0.49) showed higher adulticidal activities than others. In addition to the above two components, linalool ($LT_{50}$, min=0.91), camphor ($LT_{50}$, min=1.06), fenchone ($LT_{50}$, min=1.16), 1,8-cineole ($LT_{50}$, min=2.93), and geranyl acetate ($LT_{50}$, min=1.60) also showed higher adulticidal activity than empenthrin ($LT_{50}$, min=3.45).

• Toxicological Research
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• v.14 no.2
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• pp.183-191
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• 1998

It has been reported that 50~70% of child asthma, bronchial asthma in adult, and allergic rhinitis are caused by house dust mite. The antigen extracted from house dust mite has been used for effective treatment against allergic diseases and for clinical test. This house dust mite antigen has been entirely imported from abroad. However, the composition and content of all the antigen imported vary from a brand to other brand. Thus, we need to standardize the composition and content of the antigen by developing it domestically. We proceeded pre-clinically general pharmacological test and toxicological test that are required for the eventual human use by utilizing the house dust mite cultured in Korea. In order to obtain information on general pharmacological tests such as its toxic signs in tissues or organs which are mainly affected, we examined the effect of house dust mite on the tensions of the isolated tissues and heart rates of cardiac muscle by recording with force displacement transducer of polygragh (Glass Model 7). We determined lethality of antigen extracted from house dust mite in mice and guinea pigs. We examined acute and subacute toxicity by administrating house dust mite extract of 500, 100, 20 times of the expected clinical dose. In male and female mice and guinea pigs, given a sigle intraperitoneal dose of antigen, $LD_{50}$ values were over 5.0 $\textrm{m}{\ell}$/kg, respectively. In animals administrated with house dust mite, there were no significant change of clinical symptom, body weight, food consumption, water consumption, eye examinations, urinalysis, blood biochemistry, and histopathological examinations in any animals tested. We found no toxic effect of this house dust mite. These results show that the house dust mite cultured by us could be used in the development of medicine against allergic diseases caused by the antigen of house dust mite.