• Title/Summary/Keyword: LAMP-3

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LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the Intracellular Proliferation of Salmonella typhimurium

  • Lee, Eun-Ju;Park, Kwan-Sik;Jeon, In-Sook;Cho, Jae-Woon;Lee, Sang-Jeon;Choy, Hyun E.;Song, Ki-Duk;Lee, Hak-Kyo;Choi, Joong-Kook
    • Molecules and Cells
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    • v.39 no.7
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    • pp.566-572
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    • 2016
  • Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Salmonella-infected cells and are recruited to Salmonella-containing vacuoles (SCVs) as well as Salmonella- induced filaments (Sifs) that promote the survival and proliferation of the Salmonella. LAMP-3, also known as DC-LAMP/CD208, is a member of the LAMP family of proteins, but its role during Salmonella infection remains unclear. DNA microarray analysis identified LAMP-3 as one of the genes responding to LPS stimulation in THP-1 macrophage cells. Subsequent analyses reveal that LPS and Salmonella induced the expression of LAMP-3 at both the transcriptional and translational levels. Confocal Super resolution N-SIM imaging revealed that LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella. Knockdown of LAMP-3 by specific siRNAs decreased the number of Salmonella recovered from the infected cells. Therefore, we conclude that LAMP-3 is induced by Salmonella infection and recruited to the Salmonella pathogen for intracellular proliferation.

Illuminating Characteristics of an Incandescent Lamp (전조용 백열등의 조도 특성)

  • 김태한;이경진;이정택
    • Journal of Biosystems Engineering
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    • v.25 no.1
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    • pp.25-32
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    • 2000
  • Motivated by the need for developing the more efficient lighting system for light culture in the greenhouses, this paper aims to predict the illumination of incandescent lamp according to various levels of lighting and various kinds of the lamp. The results obtained in this experiment are summarized as follows. 1. The general equation to predict the illumination according to the kind of incandescent lamp and the installation height was suggested as {{{{y= { a + bx+ cx^2} over { 1+dx+ex^2~ + fx^3} [lx]}}}} , where , a, b, c , d, e and of were arbitrary constants. 2. Maximum illumination of hat-covered lamp of 60 W was 1.2 -1.5 times as many as uncovered lamp. 3. Maximum illumination of yellow color painted lamp of 100 W was 1.3 times as many as unpainted lamp.

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COMPARISON OF THE DECREE OF CONVERSION IN LIGHT-CURED COMPOSITE RESIN CURED BY HALOGEN AND PLASMA XENON ARC LAMP CURING UNIT (Halogen lamp 광조사기와 Plasma xenon arc lamp 광조사기에 의한 광중합 복합레진의 중합률 비교)

  • Lee, Young-Jun;Jeong, Byung-Cho;Choi, Nam-Ki;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.3
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    • pp.328-336
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    • 2002
  • Recently, new light curing unit utilizing the plasma xenon arc lamp is introduced. This curing unit is operated at relatively high intensity, so shortening the curing time significantly. The aim of this experiment was to estimate curing capability of plasma xenon arc lamp curing unit compared to traditional halogen lamp curing unit. Degree of conversion was evaluated by Raman spectroscopy after irradiation of specimens with halogen lamp curing unit(Optilux 150, Demetron, USA) for 20s, 40s, 60s and plasma xenon arc lamp curing unit(flipo, Lokki, France) for 2s, 3s, 6s. The results showed that strong light intensity of plasma xenon arc lamp curing unit did not compensate for short exposure time completely. So, Multi-layered curing within 2mm thickness and additional exposure time is recommanded when light-cured composite resin is polymerized with plasma xenon arc lamp curing unit.

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Aging-Time Dependence of Color Rendering Index of a LED Lamp for Lighting (조명용 LED 램프의 사용 시간에 따른 연색지수 특성변화)

  • Son, Minwoo;Lee, Duck Hee;Seo, Jung-Chul
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.28 no.3
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    • pp.1-6
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    • 2014
  • We measured color rendering index(CRI) of a LED lamp for lighting for aging-time. We chose bulb type white LED lamp six samples and halogen lamp one sample and applied 220V, 60Hz to all the samples for 1,000hours at ordinary temperature. The CRI was measured every 20hours and the CRI change of the LED lamp was compared with the halogen lamp's CRI change. As time goes, efficiency of the halogen lamp decreased and the CRI maintained uniformly. The other hand, efficiency of the LED lamp decreased but the CRI increased. The CRI of the LED lamp has been stabilized since 600hours. The CRI change of the LED lamp was analyzed with a spectrum, color coordinate and color temperature.

A STUDY ON LAMP BANK DESIGN OF SOLAR SIMULATOR (솔라시뮬레이터의 램프뱅크 설계에 관한 연구)

  • Baek, S.H.
    • Journal of computational fluids engineering
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    • v.17 no.3
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    • pp.45-52
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    • 2012
  • This paper is a study on the design of the solar lamp bank which is a very important part of the solar simulator with the commercial metal halide lamps and infrared lamps. Lamp Bank is designed by the lamp bank design program based on point light source theory. The reliability of the program for lamp bank design is verified through irradiance variation experiments of a kind of lamp according to horizontal distance. Solar lamp bank facilitates heat distribution and satisfies the irradiance in the three wave length which test guidelines require. The shape of the ceiling board next to the lamp bank to promote the lamp cooling efficiency and to reduce temperature deviation and air velocity deviation in the chamber is so creative. The ceiling board of partial closed type is the best among several types.

Comparison of the Effects of Luminous Lamp, and Nonluminous Lamp Radiation on Experimental Pain Threshold Sensitivity (발광·무광 적외선등 조사가 실험적 통증역치에 미치는 효과 비교)

  • Lim, In-Hyuk;Lee, Jeong-Weon;Cho, Su-Won
    • Physical Therapy Korea
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    • v.9 no.3
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    • pp.1-9
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    • 2002
  • The purpose of this study was to compare the experimental pain threshold when used in luminous lamp radiation and nonluminous lamp radiation with healthy person. Thirty normal subjects were randomly assigned two groups: a luminous lamp radiation group, and a nonluminous lamp radiation group. The infrared lamps were applied on L3 for thirty minutes. Each group was measured for experimental pain threshold and local temperature before, 15 and 30 minute radiation. For statistical differences in change of the experimental pain threshold and local temperature due to differences in lamp ray was compared using the independent t-test. And, General linear model for profile plots test was used. The results were as: 1. Local temperature was significantly increased in the nonluminous lamp group (p<.01). 2. Experimental pain thershold was significantly increased in the luminous lamp group (p<.05),(p<.01). This study indicate that luminous lamp radiation was more effects of increase experimental pain thershold than nonluminous lamp radiation. Further study is needed to compare the effects of after period radiation.

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Simple and Rapid Detection of Potato leafroll virus by Reverse Transcription Loop-mediated Isothermal Amplification

  • Ju, Ho-Jong
    • The Plant Pathology Journal
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    • v.27 no.4
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    • pp.385-389
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    • 2011
  • A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RT-LAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers) detects PLRV in less than 30 min, compared to 120 min of RT-PCR. By adding fluorescent reagent during the reaction, final products of the RT-LAMP were fluorescently visualized under UV light or could be differentiated by naked-eye inspection under normal light. The RT-LAMP was extremely sensitive, about 2000-fold more sensitive than RT-PCR. This study presents great potential of the RT-LAMP for diagnosis and PLRV epidemiology because RT-LAMP method is speedy, sensitive, inexpensive, and convenient.

Transmittance Characteristics by candlepower of Incandescent Lamp (백열등의 광력에 따른 수중 투과특성)

  • 박성욱;배봉성;안희춘;이정우;서두옥
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.4
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    • pp.293-299
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    • 2002
  • The anchovy scoop net fishery is one of the important in the South sea and coastal of Jeju of Korea. They are using incandescent lamp as a fishing lamp at night to gather anchovy shoals in the water surface. Fishing lamp (AC 100 V 500 W$\times$2~3 or AC 100 V 1 ㎾$\times$1) was installed at 1 m ahead of the prow and 1.5 m height from the water surface. The fishing lamp let anchovy shoals rise to the water surface and are attracted to bag net. On this study, the distribution of submarine illumination of 1㎾ and 2 ㎾ incandescent lamp were analyzed and discussed to investigate the ability of fishing lamp which can gather anchovy shoals effectively. The submarine illumination of incandescent lamp showed peak in wave length 690 nm. The relationship between submarine illumination (L) and water depth (Z) of 1 ㎾ and 2 ㎾ incandescent lamp in vertical light is 1 ㎾ : L = 3851. 9 $e^{-1.4587Z}$ $R^2$=0.9952 2 ㎾ : L= 8211.9 $e^{-1.2852Z}$ $R^2$=0.9977 The submarine illumination of 2 ㎾ incandescent lamp of 0~4 m layers appeared to be 3 to 4 times higher than 1 ㎾ incandescent lamp, and in more deep layers than 6 m appeared to be equal value of each lamp. The light of incandescent lamp (1 ㎾) pass through much better into vertical direction than horizontal, and submarine illumination of 20 m layers was 1.0 l$\chi$. Therefore, fishing lamp power is thought that 1 ㎾ incandescent lamp is more efficient than 2 ㎾ to gather anchovy shoals in depth of 15~20 m to water surface.

A Study on the Lamp Type Ozonizer (Lamp 형 오존발생기에 관한 연구)

  • 강천수;송현직;김상구;박원주;이광식;이동인
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1995.10a
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    • pp.25-28
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    • 1995
  • This paper describes ozone concenteration(O3con) and ozone generation(O3g) of lamp type ozo-nizer(O-Lamp)which be performed a role of lighting source and ozonizer. O=Lamp is consist of two low pressure mercury lamps. The important conclus-ions obtained from this paper are as follows, The more quality of supplied gas(Q) decrease, the higher O3con rise, The more quality of supplied gas(Q) increase, the higher O3g some rise. The Escherichia coli is reacted on ozone can be sterilized above 99[%]. Also, O-Lamp can confirmed possibility of appli-cation as lighting-source.

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Comparative Evaluation of Loop-Mediated Isothermal Amplification (LAMP) and Conventional PCR for Detection of Shiga-Toxin-Producing Escherichia coli (STEC) in Various Food Products

  • Hyejin Jang;Yong Sun Cho
    • Journal of Food Hygiene and Safety
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    • v.38 no.5
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    • pp.347-355
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    • 2023
  • In this study, polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) were compared in terms of their ability to detect shiga-toxin-producing Escherichia coli (STEC). Various foods were artificially inoculated with STEC to evaluate the limit of detection (LOD), limit of quantification (LOQ), sensitivity, specificity, and efficiency of PCR and LAMP. The LODs were ≤104 and ≤103 CFU/mL for PCR and LAMP, respectively. The LOQs did not differ between PCR and LAMP. However, of the four considered food types, the sensitivities differed by a maximum of 11.1% for seasoned meat and by a minimum of 8.1% for ground beef. LAMP had higher sensitivity than that of PCR and 100% specificity for all four food types. Therefore, LAMP is a reliable molecular method for detecting STEC as comparable to PCR assay, and its specificity and sensitivity are superior to those of PCR, depending on the food type.