• KIEAE Journal
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• v.12 no.5
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• pp.43-52
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• 2012

This study aims to analyze the green strategies of laboratory buildings in the U.S. developed by LABS21 and LEED of USGBC. To achieve this goal, the paper analyzed the design process of green laboratories and the sustainable planning strategies. Laboratories, as a building type, have specific requirments stipulated by NIH. Chemical restive measures and biosafety level measures needed to be met in laboratory buildings prior to meeting green measures. Obama Admistration's Executive Order 13514 in mind, the paper has mainly focused on the five areas of green planning strategies in the laboratory buildings; site, energy, water, indoor environment, and materials. The study informed that the current green certification program needs to expand into the particular building types in order to; first, provide more realistic energy-saving benchmarking data, and second, provide building-type-specific green strategies.

• Journal of Genetic Medicine
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• v.21 no.1
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• pp.22-30
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• 2024

Purpose: Neurofibromatosis 1 (NF1) is one of the most common autosomal dominant diseases caused by heterozygous mutation in the NF1 gene. Mutation detection is complex owing to the large size of the NF1 gene, the presence of a high number of partial pseudogenes, and the great variety of mutations. We aimed to study the mutation spectrum of NF1 gene in Korean patients with NF1. Materials and Methods: We have analyzed total 69 unrelated patients who were clinically diagnosed with NF1. PCR and sequencing of the NF1 gene was performed in all unrelated index patients. Additionally, multiplex ligation-dependent probe amplification (MLPA) test of the NF1 and SPRED1 gene analysis (sequencing and MLPA test) were performed in patients with negative results from NF1 gene sequencing analysis. Results: Fifty-five different variants were identified in 60 individuals, including six novel variants. The mutations included 36 single base substitutions (15 missense and 21 nonsense), eight splicing mutations, 13 small insertion or deletions, and three gross deletions. Most pathogenic variants were unique. The mutations were evenly distributed across exon one through 58 of NF1, and no mutational hot spots were found. When fulfilling the National Institutes of Health criterion for the clinical diagnosis of NF1, the detection rate was 84.1%. Cafe-au-lait macules were observed in all patients with NF1 mutations. There is no clear relationship between specific mutations and clinical features. Conclusion: This study revealed a wide spectrum and genetic basis of patients with NF1 in Korea. Our results aim to contribute genetic management and counseling.

• The Journal of Sustainable Design and Educational Environment Research
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• v.3 no.2
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• pp.1-21
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• 2003

This study will focus on analysing changes in subject content, teaching and learning methods, teaching model, and evaluation which have been brought about by the introduction of the 7th education curriculum, and on considering how many kinds of science labs are needed, and on proposing the desirable direction of building them. Methods for studying the goals above are as follows: First, considerations were given on how science labs must be structured and on which equipment are needed, which were done by analysing change of science education for future society, science subjects in the 7th educational curriculum, current situation of science labs, and science educational materials. Second, based on the need of students, it was examined how many science classes were needed, and through this the number of labs needed and users' requirement were analysed. Third, on the basis of the analysis above, existing conditions in planning science labs and the example of existing labs were examined.

• Journal of Dental Rehabilitation and Applied Science
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• v.21 no.2
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• pp.183-189
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• 2005

There is no single or simple answer for effective communication between laboratory technician and clinical dentist. Love-hate could be used to describe the relationship between dentists and their labs. Great lab work can make a dentists life much easier and can result in getting many referrals. However, if the lab work is not up to par and/or late, it can add stress and agony to a dentists practice. Also the opposition is right. If the preparations are calibrated, the impressions clear, and the bite registrations accurate, most labs can do their work reasonably well. Unfortunately, many cases received by labs are barely adequate, especially when it comes to reading margins, which are frequently created by the imagination of the person trimming the dies. In other words, both dentists and lab technicians need to be able to understand how each party operates so that solutions to problems can be found. Effective communication with the laboratory is critical if any degree of success is desired.

• Journal of Preventive Medicine and Public Health
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• v.38 no.4
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• pp.449-456
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• 2005

Objectives : The biosafety level (BSL) practiced in microbiology laboratories in Korea according to the laboratory biosafety manual published by the World Health Organization (WHO) was evaluated using the data obtained by a survey. Methods : Under the advise of Clinical Laboratory Physicians, 144 types of microorganisms were screened based on the guidelines of biosafety in microbiological and biomedical laboratories published by the US Center for Disease Control and Prevention and classified into 1-4 risk groups. A questionnaire containing 21 questions in 5 areas was developed using the biosafety manual by published WHO. Of the 1,876 different organizations sent the survey, 563 responded to the survey (response rate: 30.0%). The species of microoganisms handled by as well as the biosafety level in microbiology laboratories were analyzed. Results : There were 123 species of microorganisms handled in microbiology labs in Korea. The BSL required in 512 microbiology labs was answered by the survey responders as the first grade in 33 labs (6.4%), 2nd in 437 (85.4%), 3rd in 42 (8.2%), and 4th in none. The average number of items satisfied was 12.2, showing only a 57.9% satisfactory rate and normal distribution. Conclusions : The state of overall observance of BSL in most microbiology labs of Korea was evaluated as lagging compared with the standard set up by WHO. Therefore, the Korean government need to produce and distribute a biosafety manual in microbiology laboratories and make efforts to prevent this threat through measures such as training in biosafety in microbiology labs.

• Journal of the Korean Institute of Educational Facilities
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• v.21 no.5
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• pp.23-30
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• 2014

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.543-544
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• 2007

• Journal of Engineering Education Research
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• v.21 no.6
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• pp.118-127
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• 2018

Recently, universities have applied a living lab as a user-led innovation model. This study analyzed two cases, British Columbia University, and D University. They are trying to change current provider-centered and expert-centered education model which encounters the limitations. To deriving the characteristics of university living lab, we analyzed the background, goals, methods, and implications of each case. The University of British Columbia operated a living lab centered on university buit-in environment. Students and faculty members participated in the living lab as proconsumers. D University operated a living lab as part of industry - academia cooperation and regional cooperation. The local community was set up as a living lab and knowledge providers, students, and users, local citizens, solved the problem jointly. Although the methods of living labs are different from each other, they are introducing new research and education methods and utilizing participatory governance.

• Carbon letters
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• v.21
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• pp.103-106
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• 2017

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.1
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• pp.54-62
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• 2018

Objective: Due to the threat of global warming, the livestock industry is increasingly interested in exploring how feed additives may reduce anthropogenic greenhouse gas emissions, especially from ruminants. This study investigated the effect of Rhodophyta supplemented bovine diets on in vitro rumen fermentation and rumen microbial diversity. Methods: Cannulated Holstein cows were used as rumen fluid donors. Rumen fluid:buffer (1:2; 15 mL) solution was incubated for up to 72 h in six treatments: a control (timothy hay only), along with substrates containing 5% extracts from five Rhodophyta species (Grateloupia lanceolata [Okamura] Kawaguchi, Hypnea japonica Tanaka, Pterocladia capillacea [Gmelin] Bornet, Chondria crassicaulis Harvey, or Gelidium amansii [Lam.] Lamouroux). Results: Compared with control, Rhodophyta extracts increased cumulative gas production after 24 and 72 h (p = 0.0297 and p = 0.0047). The extracts reduced methane emission at 12 and 24 h (p<0.05). In particular, real-time polymerase chain reaction analysis indicated that at 24 h, ciliate-associated methanogens, Ruminococcus albus and Ruminococcus flavefaciens decreased at 24 h (p = 0.0002, p<0.0001, and p<0.0001), while Fibrobacter succinogenes (F. succinogenes) increased (p = 0.0004). Additionally, Rhodophyta extracts improved acetate concentration at 12 and 24 h (p = 0.0766 and p = 0.0132), as well as acetate/propionate (A/P) ratio at 6 and 12 h (p = 0.0106 and p = 0.0278). Conclusion: Rhodophyta extracts are a viable additive that can improve ruminant growth performance (higher total gas production, lower A/P ratio) and methane abatement (less ciliateassociated methanogens, Ruminococcus albus and Ruminococcus flavefaciens and more F. succinogenes.