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Adsorption of Heavy Metal onto the Extracellular Polysaccharide Produced by the Purple Nonsulfur Photosynthetic Bacteria Rhodopseudomonas sp. KH4 (홍색 비황 광합성 세균 Rhodopseudomonas sp. KH4의 Extracellular polysaccharide의 중금속 흡착)

  • Jeong, Jeong-Hwa;Seo, Pil-Soo;Kong, Sung-Ho;Lee, Jong-Yeol;Lee, Sang-Seob
    • Korean Journal of Microbiology
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    • v.42 no.4
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    • pp.326-331
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    • 2006
  • In the present study, we examined biosorption characteristics of heavy metals onto the extracellular polysaccharide (EPS) produced by the purple nonsulfur photosynthetic bacteria Rhodopseudomonas sp. KH4, which was isolated from a stream in Anyang, Kyonggi-Do. When Cd (100 mg/L) and Cu (100 mg/L) were added to EPS (1.0 g/L) in the optimal condition (Cd; pH 8, Cu; pH 5, $40^{\circ}C$), 84.2 mg/L of Cd and 70.0 mg/L of Cu were adsorbed within 30 min and 10 min, respectively. When 100 mg/L of Cd and Cu were present as mixture, 16.8 mg/L of Cd and 48.7 mg/L of Cu were adsorbed at $25^{\circ}C$, pH 5. The maximum adsorption capacity determined by fitting Langmuir isotherms model was suitable for describing the biosorption of Cd (76.9 mg/g) and Cu (67.1 mg/g) by EPS. The neutral monosaccharide in the EPS determined by GC consisted of arabinose (2.4%), glucose (7.1%) and mannose (90.5%).

Quality Characteristics and Antioxidant Activities of Noodles added with Spergularia marina L. Griseb Powder (세발나물 분말 첨가 국수의 품질 특성 및 항산화 활성)

  • Chang, Hyesun;Kim, Minseon;Kim, Minzy;Lee, Jisuk;Kim, Yaeboon;Sim, Ki Hyeon
    • Journal of the East Asian Society of Dietary Life
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    • v.27 no.1
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    • pp.50-60
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    • 2017
  • This study evaluated the quality characteristics and antioxidant activities of Spergularia marina L. Griseb powder from halophyte plants added to noodles at ratios of 0%, 0.5%, 1.0%, 1.5%, and 2.0% in order to examine its applicability as a substitute for salt. For quality characteristics of noodles with Spergularia marina L. Griseb powder, pH increased (p<0.05) and salinity decreased (p<0.01) with higher added Spergularia marina L. Griseb powder content. Water absorption ratio decreased (p<0.01) while turbidity decreased (p<0.001) with greater addition of Spergularia marina L. Griseb powder. However, volume expansion ratio increased with greater addition of Spergularia marina L. Griseb powder, but there was no significant difference. For chromaticity, L value and a value decreased (p<0.001) while b value increased (p<0.001) with greater addition of Spergularia marina L. Griseb powder. For texture, hardness (p<0.001), gumminess (p<0.001), and cohesiveness (p<0.01) increased with greater amount of added Spergularia marina L. Griseb powder; however, there were no significant differences in adhesiveness, springiness, and chewiness according to the amount of added Spergularia marina L. Griseb powder. In the sensory evaluation, for color, the score was 5.55 points when 1.5% Spergularia marina L. Griseb powder was added, which was the best preference (p<0.001); for flavor, preference was best (5.64 points) when 2.0% Spergularia marina L. Griseb powder was added; for taste, preference was best (5.36 points) (p<0.001) when 2.0% Spergularia marina L. Griseb powder was added; for overall preference (overall quality) the score was 5.55 points when 1.0% Spergularia marina L. Griseb powder was added, which was the best preference (p<0.001). For antioxidant activities, antioxidant activities including total phenol content, total flavonoid content, and DPPH free radical scavenging activity increased (p<0.001) with the amount of added Spergularia marina L. Griseb powder. As a result of the above research, preference was the highest and quality was excellent overall when 1.0% Spergularia marina L. Griseb powder was added. Thus, addition of 1.0% Spergularia marina L. Griseb instead of salt would be desirable.

A study on the Livestock nonpoint source runoff characteristics and Load Calculation (축산계 비점오염원 유출 특성 및 부하량 산정에 관한 연구)

  • Ryu, Jeha;Yoon, Chun Gyung;Cho, MoonSoo;Lee, HyoJun;Lee, BoMi
    • Proceedings of the Korea Water Resources Association Conference
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    • 2016.05a
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    • pp.574-574
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    • 2016
  • 유역으로 유입되는 오염물질의 발생원은 점오염원과 비점오염원으로 구분할 수 있으며 점오염원의 경우 생활하수, 산업폐수, 그리고 축산폐수에 대해 처리시설 확충 및 기술개발을 통해 관리하고 있다. 비점오염원에서의 오염물질 유출은 토지이용, 강우, 불특정적인 오염물질 투입상태 등에 따라 다르며, 지역적 특성에 영향을 받기 때문에 불확실성이 고려되어야 한다. 특히 농촌지역에서의 비점오염은 접근이 어렵고 관리주체가 모호하여 좀처럼 규명되지 않았으며, 전국적으로 그 영향이 정량화되지 않아 실질적인 관리 및 대책마련에 어려움이 있었다. 특히, 가축분뇨의 발생으로부터 처리, 자원화에 이르기까지 각 관리체계에 있어서 축산비점오염의 배출경로와 수계오염부하량, 수질환경 영향을 정밀하게 분석하여 향후 대책마련을 위한 기초자료를 확보할 필요가 있다. 또한 전국적으로 비점오염이 수계에 미치는 영향과, 그 중 축산비점오염의 영향을 면밀히 분석하여 향후 정책 및 제도개선을 위한 과학적 기초자료로서 활용할 필요가 있다. 따라서 본 연구에서는 축산 밀집 지역을 대상으로 오염원 조사를 통해 강우시 비점오염 모니터링 지점을 선정 하였으며, 년5회씩 강우 모니터링을 하여 기초데이터를 축적 하였다. 대상지역은 강원도 횡성군에 위치한 일리천 유역이며 농가 수는 총 90개의 농가가 위치하고 있는데 그 중 돼지 1,467마리, 한우 1,957마리, 젖소 581마리, 개 2,880마리, 닭 75,000마리, 사슴 4마리로 조사되었다. 대상유역을 대상으로 배출부하량을 조사한 결과 BOD 배출부하량은 총 509.3 kg/day, T-N 배출부하량은 총 331.5 kg/day, T-P 배출부하량은 총 28.3 kg/day로 조사되었다. 유출특성을 파악하기 위하여 유량가중평균농도(Event Mean Concentration, EMC)를 산정한 결과 BOD의 경우 MW-4에서 1.2 mg/L - 7.2 mg/L, MW-5에서 0.8 mg/L - 6.3 mg/L, MW-7에서 0.7 mg/L - 5.2 mg/L의 범위를 보였다. T-N의 경우 MW-4에서 1.426 mg/L - 5.321 mg/L, MW-5에서 1.205 mg/L - 4.27 mg/L, MW-7에서 0.989 mg/L - 3.859 mg/L의 범위를 보였다. T-P의 경우 MW-4에서 0.245 mg/L - 0.632 mg/L, MW-5에서 0.236 mg/L - 0.596 mg/L, MW-7에서 0.213 mg/L - 0.521 mg/L의 범위를 보였다. 본 연구에서 EMC를 산정한 방법은 평시 수질 및 유량을 정하는 기준에 따라 값이 많이 달라질 수 있다. 따라서 합리적으로 평시 부하량을 제외하고 강우시의 영향을 파악할 수 있는 EMC 산정방법에 대한 추가적인 고찰이 필요할 것으로 사료된다.

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Resistance to Reactive Oxygen Species and Antioxidant Activities of Some Strains of Lactic Acid Bacteria from the Mustard Leaf Kimchi (갓김치에서 분리된 유산균의 활성산소종에 대한 저항성과 항산화 활성)

  • Lim, Sung-Mee
    • Korean Journal of Microbiology
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    • v.46 no.4
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    • pp.375-382
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    • 2010
  • In present study, five strains of Lactobacillus acidophilus GK20, Lactobacillus brevis GK55, Lactobacillus paracasei GK74, Lactobacillus plantarum GK81, and Leuconostoc mesenteroides GK104 isolated from the mustard leaf kimchi were investigated for resistance to reactive oxygen species (ROS) and antioxidant activity. L. acidophilus GK20, L. brevis GK55, L. paracasei GK74, and L. plantarum GK81 were resistant to hydrogen peroxide (0.5 mM), showing a survival rate of 50% or more. In particular, L. acidophilus GK20 and L. paracasei GK74 were the most superoxide anions-resistant and L. paracasei GK74 and L. plantarum GK81 were most likely survive hydroxyl radicals. Meanwhile, the intracellular cell-free extract (ICFE) from L. plantarum GK81 exhibited significantly higher DPPH radical scavenging values ($96.4{\pm}2.8%$) than the intact cells (IC). The ICFE of L. plantarum GK81 showed the highest superoxide radical scavenging ability and chelating activity for $Fe^{2+}$ ions among the 5 lactic acid bacteria (LAB) tested, and IC and ICFE from L. plantarum GK81 demonstrated excellent reducing activity, which was higher than those of BHA and vitamin C as a positive control.

Health-related Quality of Life of Patients With Diabetes Mellitus Measured With the Bahasa Indonesia Version of EQ-5D in Primary Care Settings in Indonesia

  • Muhammad Husen Prabowo;Ratih Puspita Febrinasari;Eti Poncorini Pamungkasari;Yodi Mahendradhata;Anni-Maria Pulkki-Brannstrom;Ari Probandari
    • Journal of Preventive Medicine and Public Health
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    • v.56 no.5
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    • pp.467-474
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    • 2023
  • Objectives: Diabetes mellitus (DM) is a serious public health issue that places a heavy financial, social, and health-related burden on individuals, families, and healthcare systems. Self-reported health-related quality of life (HRQoL) is extensively used for monitoring the general population's health conditions and measuring the effectiveness of interventions. Therefore, this study investigated HRQoL and associated factors among patients with type 2 DM at a primary healthcare center in Indonesia. Methods: A cross-sectional study was conducted in Klaten District, Central Java, Indonesia, from May 2019 to July 2019. In total, 260 patients with DM registered with National Health Insurance were interviewed. HRQoL was measured with the EuroQol Group's validated Bahasa Indonesia version of the EuroQoL 5-Dimension 5-Level (EQ-5D-5L) with the Indonesian value set. Multivariate regression models were used to identify factors influencing HRQoL. Results: Data from 24 patients were excluded due to incomplete information. Most participants were men (60.6%), were aged above 50 years (91.5%), had less than a senior high school education (75.0%), and were unemployed (85.6%). The most frequent health problems were reported for the pain/discomfort dimension (64.0%) followed by anxiety (28.4%), mobility (17.8%), usual activities (10.6%), and self-care (6.8%). The average EuroQoL 5-Dimension (EQ-5D) index score was 0.86 (95% confidence interval [CI], 0.83 to 0.88). In the multivariate ordinal regression model, a higher education level (coefficient, 0.08; 95% CI, 0.02 to 0.14) was a significant predictor of the EQ-5D-5L utility score. Conclusions: Patients with diabetes had poorer EQ-5D-5L utility values than the general population. DM patients experienced pain/discomfort and anxiety. There was a substantial positive relationship between education level and HRQoL.

Water Quality of Some Spring Waters in Pusan Area (부산시내에 산재하는 몇몇 약수터 약수의 수질)

  • KIM Yong-Gwan;CHO Hyeon-Seo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.6
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    • pp.538-544
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    • 1985
  • This study was carried cut to evaluate the water quality of spring waters in Pusan area(see Fig. 1). In this experiment, twenty-five water samples were collected from 5 stations from December 1983 to August 1984. Range and mean values of constituents of the samples are as follows: pH $5.80{\sim}7.25$, 6.60; water temperature $6.0{\sim}23.0^{\circ}C,\;12.9^{\circ}C$; total residue $33.0{\sim}325mg/l$, 121.2mg/l; alkalinity $4.75{\sim}51.6mg/l$, 24.1mg/l; hardness $9.47{\sim}85.0mg/l$, 30.3mg/l; electrical conductivity $0.495{\sim}2.750{\times}^2{mu}{\mho}/cm,\;1.239{\times}10^2{\mu}{\mho}/cm$;turbidity $0.54{\sim}7.80$NTU, 2.04NTU; $KMnO_4$ consumed $0.51{\sim}8.47mg/l$, 1.96mg/l; chloride ion $4.91{\sim}36.0mg/l$, 12.55mg/l; fluoride ion ND-0.30ppm, 0.08ppm; nitrate-nitrogen ND-8.94mg/l, 1.94m:g/l; nitrite-nirogen ND-0.10mg/l, 0.03mg/l; ammonia-nitrogen ND-0.16mg/l, 0.03mg/l: phosphate-phosphorus ND-0.09mg/l, 0.03mg/l; silicate-silicious $0.42{\sim}22.7ng/l$, 7.96mg/l; copper ND-10.5ppb, 2.46ppb; lead ND-22.7ppb, 3.54ppb; zinc ND-103ppb, 21.33ppb; iron $20.3{\sim}2,800ppb$, 801.72ppb, respectively. Arsenic, cyan, cadmium, manganese, mercury, chrome and phenol were not detected. Total residue, electrical conductivity, turbidity and chloride ion of station 1 (Milrakdong) were higher than others as 178.1mg/l, $2.127{\times}10^2{\mu}{\mho}/cm$, 3.16NTU and 16.32mg/l. The concentration of silicious had a great influence on precipitation. The concentration of fluoride ion of spring waters was lower as 0.08ppm than the criterion for drinking water as 1ppm, while iron was exceed 2.7 times as 801.72ppb.

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Exploratory Study of Dimensions of Health-related Quality of Life in the General Population of South Korea

  • Kim, Seon-Ha;Jo, Min-Woo;Ock, Minsu;Lee, Sang-il
    • Journal of Preventive Medicine and Public Health
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    • v.50 no.6
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    • pp.361-368
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    • 2017
  • Objectives: This study aimed to explore dimensions in addition to the 5 dimensions of the 5-level EQ-5D version (EQ-5D-5L) that could satisfactorily explain variation in health-related quality of life (HRQoL) in the general population of South Korea. Methods: Domains related to HRQoL were searched through a review of existing HRQoL instruments. Among the 28 potential dimensions, the 5 dimensions of the EQ-5D-5L and 7 additional dimensions (vision, hearing, communication, cognitive function, social relationships, vitality, and sleep) were included. A representative sample of 600 subjects was selected for the survey, which was administered through face-to-face interviews. Subjects were asked to report problems in 12 health dimensions at 5 levels, as well as their self-rated health status using the EuroQol visual analogue scale (EQ-VAS) and a 5-point Likert scale. Among subjects who reported no problems for any of the parameters in the EQ-5D-5L, we analyzed the frequencies of problems in the additional dimensions. A linear regression model with the EQ-VAS as the dependent variable was performed to identify additional significant dimensions. Results: Among respondents who reported full health on the EQ-5D-5L (n=365), 32% reported a problem for at least 1 additional dimension, and 14% reported worse than moderate self-rated health. Regression analysis revealed a $R^2$ of 0.228 for the original EQ-5D-5L dimensions, 0.200 for the new dimensions, and 0.263 for the 12 dimensions together. Among the added dimensions, vitality and sleep were significantly associated with EQ-VAS scores. Conclusions: This study identified significant dimensions for assessing self-rated health among members of the general public, in addition to the 5 dimensions of the EQ-5D-5L. These dimensions could be considered for inclusion in a new preference-based instrument or for developing a country-specific HRQoL instrument.

Optimization of Culture Conditions for D-Tagatose Production from D-Galactose by Enterobacter agglomerans. (Entrobacter agglomerans에 의한 D-Galactose로부터 D-Tagatose 생산조건의 최적화)

  • 오덕근;노회진;김상용;노봉수
    • Microbiology and Biotechnology Letters
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    • v.26 no.3
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    • pp.250-256
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    • 1998
  • D-Tagatose production from D-galactose was investigated using 35 type strains of American Culture Type Collection (ATCC) and Korean Collection for Type Cultures (KCTC) which have potential to produce D-tagatose. Enterobacter agglomerans ATCC 27987 was selected as a D-tagatose producing strain due to its short fermentation time and high production of D-tagatose. Optimization of the culture conditions for D-tagatose production by E. agglomerans ATCC 27987 was performed. Among various carbon sources, D-galactose was the most effective carbon source for D-tagatose production. As the D-galactose concentration was increased, cell growth and D-tagatose production increased. Effect of nitrogen sources on D-tagatose production was studied. Of inorganic nitrogen sources, ammonium sulfate was effective one for D-tagatose production and yeast extract was the most suitable organic nitrogen nutrient. The concentrations of inorganic compounds such as KH$_2$PO$_4$, K$_2$HPO$_4$, and MgSO$_4$$.$7H$_2$O were also optimized for D-tagatose production. The optimal medium was determined to contain D-galactose of 20 g/l, yeast extract of 5.0 g/l, (NH$_4$)$_2$SO$_4$ of 2.0 g/l, KH$_2$PO$_4$ of 5.0 g/l, K$_2$HPO of 5.0 g/l, and MgSO$_4$$.$7H$_2$O of 5 mg/l. The optimal environmental conditions in a 250-$m\ell$ flask were found to be pH of 6.0, temperature of 30$^{\circ}C$, and agitation speed of 150 rpm. D-tagatose of 0.41 g/l could be obtained in 24 h from 20 g/l D-galactose at the optimal culture condition without induction and cell concentration.

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In Vitro Morphogenesis through Leaf Explants of Gypsophila paniculata L.

  • Jo, Man-Hyun;Ham, In-Ki;Song, Nam-Hyun
    • Plant Resources
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    • v.3 no.2
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    • pp.135-137
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    • 2000
  • Callus cultures from leaf explants of Gypsophila paniculata L. cv. 'Bristol Fairy' have been tested their growth and morphogenic capacity on Murashige and Skoog medium supplemented with 0.l, 0.5, 1 and 3 mg/L 2,4-D. The frequency of callus formation ranged from 43.3% to 100%. The optimal 2,4-D concentration for promoting callus formation and growth was 0.5 to 3 ㎎/L. 4.2∼ 5.6% of adventitious roots were obtained with the use of 0.1 and 0.5 mg/L 2,4-D. Calli grown well on 1.0 mg/L 2,4-D was the heaviest among the calli grown in various concentrations.

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Chirospecific Synthesis of D-erythro- and L-threo-Sphinganines from Sugars

  • Jeong, Ill-Yun;Lee, Jin-Hwan;Lee, Byong-Won;Kim, Jin-Hyo;Park, Ki-Hun
    • Bulletin of the Korean Chemical Society
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    • v.24 no.5
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    • pp.617-622
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    • 2003
  • D-erythro-sphinganine 1 and L-threo-sphinganine 2 have been prepared in the enantiomerically pure form by the chirospecific manner. Key intermediates, 2-amino-3-hydroxy-4-pentenoates 8 and 12, were obtained from L-glucono-1,5-lactone and L-gulonic acid g-lactone via a simultaneous dealkoxyhalogenation.