• Food Science and Biotechnology
• /
• v.18 no.1
• /
• pp.162-166
• /
• 2009

This study evaluated glucose effect on Listeria monocytogenes survival under gamma irradiation and NaCl stress. L. monocytogenes in phosphate buffered saline (PBS) plus glucose (0-4%) was treated with gamma irradiation (0-0.5 kGy), and the samples were then exposed to NaCl (0-9%) in tryptic soy agar plus 0.6% yeast extract. $D_{10}$ and $t_{3D}$ values were determined, and a model for prediction of $D_{10}$ values was developed. Cell counts of L. monocytogenes reduced as irradiation dose increased, and L. monocytogenes in PBS (no glucose) was more sensitive to irradiation and NaCl compared to those in PBS (2 or 4% glucose). $D_{10}$ values were 0.07-0.1, 0.12-0.16, and 0.13-0.15 kGy for 0, 2, and 4% glucose, respectively. The $t_{3D}$ values were 0.22-0.3 (0% glucose), 0.35-0.48 (2% glucose), and 0.40-0.44 (4% glucose). A model performance was acceptable. These results indicate that glucose in foods would increase the resistance of L. monocytogenes to gamma irradiation and NaCl stress.

• Korean Journal of Clinical Laboratory Science
• /
• v.45 no.1
• /
• pp.37-42
• /
• 2013

The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of $0{\sim}5WBCs/{\mu}L$ of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of $0{\sim}5WBCs/{\mu}L$ on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0~40 cells in the 44 samples of $0WBC/{\mu}L$, and 3~95 cells in the 31 samples of $1WBC/{\mu}L$. It was observed that the nucleated cell number for cells recovered on slide was 13~100 cells in the 44 samples of $2WBCs/{\mu}L$, and more than 100 cells in the 29 samples of $3{\sim}5WBCs/{\mu}L$, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of $0{\sim}5WBCs/{\mu}L$. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as $1WBC/{\mu}L$ in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at $0WBC/{\mu}L$ by using the cell yield in a comparison between the value of $0{\sim}5WBCs/{\mu}L$ and nucleated cell number for cells recovered on slide.

• Korean Journal of Food Science and Technology
• /
• v.45 no.1
• /
• pp.104-110
• /
• 2013

This study assessed hazards at the harvest stage of strawberry farms which may cause risk to humans. A total of 216 samples were collected from 6 strawberry farms (soil culture farms: A, B, C; nutriculture farms: D, E, F) located in Western Gyeongnam. The collected samples were subjected for sanitary indicator bacteria (aerobic plate count, coliforms and Escherichia coli), major foodborne pathogens (E. coli O157:H7, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus and Bacillus cereus), and fungi. The levels of APC and coliform in the soil culture farms were 1.0-6.9 and 0.4-4.6 log CFU/g (leaf, mL, hand or 100 $cm^2$), respectively. The samples obtained from the nutriculture farms were contaminated with the levels of 0.8-4.9, and 0.2-2.6 log CFU/g (leaf, mL, hand or 100 $cm^2$) of APC and coliform. However, E. coli was not detected in any samples. In major foodborne pathogens, S. aureus was detected at the level of ${\leq}$3.3 log CFU/hand in workers' hand samples and B. cereus was detected at the levels of 0.4-4.1 log CFU/g (hand or 100 $cm^2$) in soil, plants and workers' hygiene. L. monocytogenes, E. coli O157:H7 and Salmonella spp. were not detected. Fungi were detected at the levels of 1.0-5.2 and 0.2-4.4 log CFU/g (leaf, mL, hand or 100 $cm^2$) in soil culture and nutriculture farms, respectively.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.28 no.10
• /
• pp.1483-1491
• /
• 2004

To assess the integrity of the pipeline is the most important problem to be solved first of all for prevention of any fracture accident of the pipeline. As a result of exerting such efforts, a number of plastic collapse assessment equations have been suggested, however, the scope of using or applying such assessment equations has not been exactly defined. In this study, the case that a surface crack existed in the circumferential direction in the external side of the natural gas pipeline and a bending load was applied to the pipeline was analytically identified as the most critical condition, and a plastic collapse assessment equation fur it was suggested. The flow stress of the API X65 linepipe was defined through the experiment conducted on SENT specimens. Also, a local assessing criterion of a 3-dimensional crack behavior considering not only the crack depth but also the crack length was suggested. Finally, a plastic collapse assessment equation for the API X65 linepipe was developed by performing the 3-dimensional finite element analysis.

• Journal of the Korean Applied Science and Technology
• /
• v.23 no.3
• /
• pp.264-271
• /
• 2006

PU type flame-retardant coatings (TBAO/L-75, TBAOL ; TBAO/N-100, TBAON) were prepared by blending bromine-containing modified polyester (TBAO) which was synthesized in our earlier work. with two kinds of isocyanate curing agents, Desmodur L-75 and Desmodur N-100. Physical properties of the prepared flame-retardant coatings were tested. TBAOL shows better hardness than TBAON, while TBAON shows better viscosity, accelerated weathering resistance, yellowness index and lightness index difference than TBAOL. There were no remarkable differences in fineness of grind, $60^{\circ}$ specular gloss, cross-hatch adhesion, and abrasion resistance of TBAOL and TBAON. There was no discernable difference in flame-retardancy between the two flame-retardant coatings, TBAOL and TBAON. When the content of tribromo acetic acid, which is flame-retarding component, was 30wt% the LOI value was in a range of $29{\sim}30%$, which indicates that the two coatings are good flame-retardant coatings.

• Horticultural Science & Technology
• /
• v.33 no.2
• /
• pp.251-258
• /
• 2015

Eurycoma longifolia is an important rare medicinal plant that contains valuable bioactive compounds. In the present study, cell suspension culture of E. longifolia was established for the production of biomass and phenolic compounds. Various medium parameters, such as concentration of auxin, salt strength of the medium, and sucrose and nitrogen concentrations, were optimized for the production of biomass at the flask-scale level. Full strength Murashige and Skoog (MS) medium supplemented with $3.0mg{\cdot}L^{-1}$ naphthaleneacetic acid (NAA), 3% (w/v) sucrose, 0:60 $NH{_4}^+:NO{_3}^-$ was found suitable for biomass accumulation. Based on the optimized flask-scale parameters, cell suspension cultures were established in balloon-type bubble bioreactors, and bioprocess parameters such as inoculum density and aeration rate were optimized. Inoculum density of $50g{\cdot}L^{-1}$ and increasing aeration rate from 0.05 to 0.3 vvm, with increases every 7 days, were suitable for the accumulation of both biomass and phenolic compounds. With the optimized conditions, $14.70g{\cdot}L^{-1}$ dry biomass, $10.33mg{\cdot}g^{-1}$ DW of phenolics and $3.89mg{\cdot}g^{-1}$ DW of flavonoids could be achieved. Phenolics isolated from the cell biomass showed optimal free radical scavenging activity.

• The Journal of Korean Academy of Prosthodontics
• /
• v.33 no.4
• /
• pp.634-644
• /
• 1995

The purpose of this study is to evaluate the color differences in the In-Ceram according to coping thickness and various abutment core materials ; amalgam, precious alloy, composite resin, non-precious alloy. After the porcelain was built up on the In-Ceram coping at the thicknes of 0.3mm and 0.5mm then it was cemented with glass ionomer cement to the post & core materials. The following results were obtained. 1. There were significant differences in the $L^*$ values $a^*$ and $b^*$ values in the cementation of different cores, to the In-Ceram(P<0.01). $L^*$ values were not significantly different between the composite resin to the In-Ceram and the $a^*\;b^*$ values were not significantly different between the amalgam and the non-precious alloy. 2. All of cementations of In-Ceram to the core materisal had color difference(${\Delta}E^*ab$) compared to the In-Ceram. In the 0.3mm thickness of the In-Ceram copping non-precious alloy indicated the greatest value, while the composite resin core showed the lowest value with a thickness of 0.5mm In-Ceram copping. 3. By controlloing the In-Ceram coping thickness $L^*$ value was significatly different(P<0.01), but not in $a^*$ and $b^*$ values. 4. In an amalgam, precious & non-prcious alloys there was a 1,74 to 3.06 range color difference in the controlled thickness of In-Ceram coping at the thickness of 0.3mm and 0.5mm. The above results suggest that the requirement of the sufficient thickness of In-Ceram coping and the suitable core material in order to get an estheti restoration by In-Ceram and also to intercept the original core color.

• Clinical and Experimental Pediatrics
• /
• v.49 no.5
• /
• pp.533-538
• /
• 2006

Purpose : The headache, one of the symptoms of meningitis, is related to abrupt elevation of intracranial pressure(ICP) or stimulation of intracranial nociceptive structure. However, in cases of mild elevation of ICP or normal findings of cerebrospinal fluid(CSF) analysis, patients sometimes complain of headaches. Therefore, other pathways may contribute to the occurrence of headaches in aseptic meningitis or meningismus. We intend to investigate the role of substance P(SP) and calcitonin gene-related peptide(CGRP) in aseptic meningitis or meningismus. Methods : We measured leukocyte count, the concentration of protein and glucose in CSF and ICP of patients with meningeal irritation sign. We also measured SP and CGRP levels by using immunoassay. We analyzed the relationship between the presence of headache and the value of SP and CGRP. Results : The concentrations of CGRP($18.8{\pm}10.5ng/mL$) in CSF and ICP($14.8{\pm}4.5cmH_2O$) in aseptic meningitis group were significantly higher than in those($14.1{\pm}7.4ng/mL$ and $12.0{\pm}5.1cmH_2O$, respectively) of the meningismus group(P<0.05). There was no significant difference in the SP levels between the two groups. In the aseptic meningitis group, the concentrations of SP and CGRP were significantly higher in the normal ICP group than in the elevated ICP group(P<0.05). Conclusion : Headaches in children with aseptic meningitis or meningismus is considered to be related to the elevation of the CSF levels of SP and CGRP.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.41 no.3
• /
• pp.155-162
• /
• 2008

The seawater in the Taean area was surveyed to evaluate the conditions of the bay and compliance with the bacteriological criteria for a designated area for shellfish production for export. Samples of seawater were collected monthly at 34 sampling stations established in the survey area from January 2002 to December 2004. The bacterial density in the coastal area close to a pollution source located to the northeast of the survey area was higher than in the open sea to the west. The bacteriological counts in the water did not change with 16.5mm of rainfall, but increased abruptly after 65.4mm of rainfall. The total coliform and fecal coliform most probable numbers (MPNs) of 1,224 seawater samples in the survey area were <1.8-2,400 and <1.8-790 MPN/100 mL, respectively. The geometric mean and estimated 90th percentile of total coliforms were 1.9-3.4 and 2.7-26.3 MPN/100mL, respectively, and for fecal coliforms were <1.8-2.6 and 1.8-12.0 MPN/100mL, respectively. The bacteriological water quality in the Taean seawater area met the National Shellfish Sanitation Program criteria for an approved area and the Korea Shellfish Sanitation Program criteria for a designated area for shellfish production for export.

• The Korea Journal of Herbology
• /
• v.23 no.3
• /
• pp.19-25
• /
• 2008

Objectives : This study was performed to evaluate the neuroprotective effect of water extracts from Thujae Semen(TSW) in PC12 cells. Methods : We performed 2,2-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging assay, 2,2-azinobis- (3-ethyl-benzothiazoline-6-sulfonic acid(ABTS) cation scavenging assay, and determination of total polyphenolic content to examine the antioxidant effects of TSW. We also carried out 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay(MTT), reactve oxygen species(ROS) assay, and nitric oxide(NO) assay to examine neuroprotective effects against 6-hydroxydopamine(6-OHDA) in PC12 cells. Results : TSW showed $IC_{50}$ values of 404.3 and 219.9 ${\mu}g/mL$ in DPPH and in ABTS assays, respectively. TSW showed 9.74 ${\mu}g/mL$ of total polyphenol contents. TSW incresed cell viability in a dose dependent manner and it showed protective effect against 6-OHDA neurotoxicity at the concentration of 25-200 ${\mu}g/mL$. Moreover, it recovered 6-OHDA induced cell death at the same concentrations. The extract showed a dose dependent reduction of ROS and NO generation by 6-OHDA. Conclusions : We concluded that TSW has neuroprotective effect against 6-OHDA-induced toxicity in PC12 cells through ROS and NO inhibition.