• Food Science and Biotechnology
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• 제17권3호
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• pp.623-630
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• 2008

A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.283-289
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• 2009

We evaluated total phenolics and antioxidative activities of water extracts from different parts of Cudrania tricuspidata (specifically, the leaves, stems, roots, and fruits). The antioxidative activities of these samples were determined using five methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging, reducing power, ferric reducing ability of plasma (FRAP), and $\beta$-carotene/linoleic acid system. The water extract of leaves exhibited the higher DPPH, ABTS radical scavenging activities, reducing power, and FRAP than water extract of stem, roots, and fruits. Inhibition values on linoleic oxidation of water extracts from leaves, stems, roots, and fruits were calculated as 45.98%, 33.03%, 39.73%, and 25.48% at 10 mg/mL, respectively. The water extract of C. tricuspidata leaves had the highest amount of toal phenolics (73.60$\pm$0.28 mg/g). High-performance liquid chromatography (HPLC) analysis showed that quercetin is the predominant phenolic compound in water extract of leaves. Thus, our study verified that the water extract of leaves has strong antioxidant activities which are correlated with its high level of phenolic compounds, particularly quercetin. This water extract of C. tricuspidata leaves can be used as an effective and safe source of antioxidants.

• 한국광학회지
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• 제19권6호
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• pp.429-434
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• 2008

비대칭 이중-금속 장거리 표면-플라즈몬 구조는 두 금속 사이에 위치한 코어 유전체 층의 굴절률을 임의로 선택하여도 장거리 표면-플라즈몬 모드가 존재한다는 장점이 있다. 도파로의 코어 층에 격자를 형성함으로써 플라즈몬 밴드-갭에 기초한 통신대역 파장에서의 장거리 표면-플라즈몬 파장필터를 구현하였다. Method of Line과 전달행렬방법 등을 통해 장거리 표면-플라즈몬 모드의 유효굴절률, 모드 특성, 투과도 등의 분석을 통해 설계하였으며, 도파로의 코어 층에 홀로그램 리소 방식을 이용하여 523.3 nm 주기의 브래그격자를 형성하여 파장필터를 제작하여 통신대역인 1541 nm 파장에서 대략 2 nm의 반치폭과 50 dB 이상의 파장 소광률을 확인하였다. 본 연구에서 제안한 파장필터는 표면-플라즈몬 집적회로를 구성하는 소자로써 중요한 역할을 할 것이다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.37-37
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• 2015

Kimchi cabbage (Chinese cabbage), radish and Cabbage are major Brassicaceae vegetables in Korea. Especially, we can easily develop whole plant from one microspore in Kimchi cabbage. To develop clubroot resistant doubled-haploid (DH) inbred lines, we pollinated a clubroot resistant turnip of 'IT 033820' with a Kimchi cabbage (Chinese cabbage) inbred of 'BP 079'. More than 85 DH inbred lines were developed from this combination. We screened about 400 materials including these DH inbred lines, commercial cultivars and breeding materials during 3 years using hydroponic system after inoculating single spore isolation race 4(SSI-04) inoculate. One inbred line derived from this combination selected as clubroot resistant and registered as 'Wonkyo20036ho'. We inoculated 26 DH inbred lines derived from 'Zoong-baek 2ho' using SSI-4, the percent of resistant plants varied from 0 to 83%. However the horticultural traits of highly resistant DH inbred line was poor. Thus we selected one DH line showing 77% resistant with yellow inner leaf and maid good head, was registered as 'Wonkyo20034ho'. Another DH inbred line derived from Korean variety of 'Wol-dong' showing 86% resistant was registered as 'Wonkyo20037ho'. Other DH inbred lines were derived from Chinese cultivar of 'Choon-hi-go-hang-wang' and 'Hwang-shim-zo48' showed 80 and 71% resistant, respectively, was also selected for registration. Even though DH inbred lines derived from turnip showed highly resistant to SSI-04 and provincial inoculate, they showed poor characteristics in horticultural traits. However, commercial seed companies showed interesting for adapting these DH inbred lines in commercial breeding.

• 한국식품영양과학회지
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• 제43권11호
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• pp.1724-1730
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• 2014

본 연구는 시판되고 있는 복숭아 통조림을 선택하여 복숭아 페이스트를 만든 후 0, 5, 10 또는 20%를 첨가한 막편을 제조하여 품질 특성 및 항산화성을 분석하였다. 복숭아 첨가량이 증가할수록 pH는 낮아지고 산도는 증가하였다(P<0.05). 복숭아 첨가량이 증가할수록 막편의 환원당이 증가하였다(P<0.05). 색도는 복숭아 첨가량이 증가할수록 명도와 적색도는 감소하고, 황색도는 증가하였다(P<0.05). 총페놀 함량은 복숭아 첨가량이 증가할수록 증가하였다. DPPH 라디칼 소거능 및 hydroxyl 라디칼 소거능은 복숭아 첨가량이 증가할수록 $IC_{50}$ 값이 낮아져 항산화능이 높았다. 기호도는 복숭아를 첨가할수록 외관, 향미, 맛, 조직감, 전체적인 기호도가 증가하는 것으로 나타났다. 특히 전체적 기호도는 복숭아 20% 첨가군이 가장 높은 점수를 받았다(P<0.05). 이상의 결과로 보아 복숭아 첨가가 떡의 항산화능과 기호도를 증가시켜 저장성이 낮은 복숭아의 활용도를 높이고 설기떡의 가치를 높일 수 있을 것으로 사료된다. 복숭아 20%를 첨가하여 복숭아 막편을 제조할 경우 전반적인 기호도와 항산화능에서 가장 적합할 것으로 판단된다. 향후 다양한 복숭아 통조림과 막걸리 제품을 이용한 비교 분석으로 최대 가치를 창출하는 복숭아 막편에 대한 연구도 필요하리라 생각된다.

• 한국환경복원기술학회지
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• 제7권5호
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• pp.85-93
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• 2004

This study was carried out to find out the effect of cool-season turfgrass overseeding onto zoysiagrass(Zoysia japonica L.) for extending green color and solving traffic problem during dormant periods. Overseeding rates of cool-season turfgrasses were $10g/m^2$ of Kentucky bluegrass(KB1), $20g/m^2$of Kentucky b1uegrass(KB2), $60g/m^2$ of perennial ryegrass(PR), $80g/m^2$ of Tall fescue(TF), and $10g/m^2$ of Kentucky bluegrass + $10g/m^2$ of perennial ryegrass mixture(KB+PR) onto 2m${\times}$2m(width${\times}$length) zoysiagrass plots with 3 replications.1 to 9-sca1e of visual quality and visual color were measured after treatment, Consequently cool-season turfgrass overseeding gave a good performances of extending the green periods and enhancing the turf grass quality. KB1 and KB2 were found to be excellent performances for visual quality and visual color compared to PR, TF, KB+PR and control. KB2 showed better turf performances than KB1 within Kentucky bluegrass overseedings. Turfgrass color was kept up for 10 months by overseeding of cool-season turfgrasses(KB1, KB2, PR, TP, KB+PR) compared to about 5~6 months by zoysiagrass(Control). The visual quality and visual color of PR and TF showed good performances during a green-up period of spring, but decreasing tendencies through the summer.Percent diseases data revealed also higher ratings for PR and TF compared to for KB1 and KB2.

• Mycobiology
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• 제45권2호
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• pp.105-109
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• 2017

Sixteen genomic DNA simple sequence repeat (SSR) markers of Lentinula edodes were developed from 205 SSR motifs present in 46.1-Mb long L. edodes genome sequences. The number of alleles ranged from 3-14 and the major allele frequency was distributed from 0.17-0.96. The values of observed and expected heterozygosity ranged from 0.00-0.76 and 0.07-0.90, respectively. The polymorphic information content value ranged from 0.07-0.89. A dendrogram, based on 16 SSR markers clustered by the paired hierarchical clustering' method, showed that 33 shiitake cultivars could be divided into three major groups and successfully identified. These SSR markers will contribute to the efficient breeding of this species by providing diversity in shiitake varieties. Furthermore, the genomic information covered by the markers can provide a valuable resource for genetic linkage map construction, molecular mapping, and marker-assisted selection in the shiitake mushroom.

• 가정∙방문간호학회지
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• 제9권1호
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• pp.5-13
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• 2002

This study was conducted to assess need for education on the infection control by examining the knowledge on infection control in home care of the home care trainees who had completed an infection control course. Using 64 items questionnaire. home care trainees of eight home care education institutions who took classes related with infection control. The level of knowledge evaluated subjectively was 4.24point (full mark: 5 point). The correct answer rates for handwashing and gloving. cleaning patients' rooms. preventing sharp injury, preventing exposure to potential infectious agents, home care bag technique were 84.5% 86.4% 70.7% 65.3% 76.2% representatively. For the knowledge on the infection control principles to prevent catheter related infection, indwelling catheter related infection, tracheal tube related pneumonia. L-tube related infection, oxygen therapy related infection. and wound infection were 62.8% 27.8% 39.1% 87.8% 76.5% 80.5% representatively. The correct answer rates varied depending upon the educational institutes (p< = .0001), educational levels (p= .001), workplaces (p<.0001), and the experience of infection control education (p= .001). Considering these results. a standardized curriculum must be developed and implemented after analyzing the existing infection control curricula of the home care education institutes. and guide books for infection control in home care should be developed and distributed. In addition. continuous infection control education be provided to the home care nurses through reeducation so they can acquire new knowledge needed for carrying out infection control activities effectively.

• 한국안광학회지
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• 제3권1호
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• pp.217-222
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• 1998

$SiO_2-TiO_2-AgO$ 코팅박막을 졸-겔법으로 현미경용 슬라이드 유리판과 씰리콘 기판에 제조하였다. 이 박막의 열처리온도는 $500^{\circ}C$가 적절하였으며 5회 반복코팅한 박막의 두께는 310nm였다. AgO가 10mol% 첨가된 박막의 표면은 균일한 구조를 보였으나 첨가량이 많아질수록 박막의 표면에 pin hole과 AgO의 석출에 기인한 cluster를 보였다. 열처리온도의 증가에 따라 IR흡수의 정도가 점차 감소하였고, AgO 첨가량의 증가에 따라 반사율은 점차 감소하였으며 색상은 light yellow에서 무채색 쪽으로 점차 전이하였다.

• 복식
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• 제16권
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• pp.173-185
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• 1991

We measured change of peripheral skin temperature and mean skin temperature when the upper arm and thigh pressured in order to know the effect of skin pressure applied by clothing on blood circulation. After release from pressure, we observed also recovery condition. At the same time, we examined relation between pressure and a feeling of tightness. Three physiques of healthy females, namely slender, standard and plump, served as subjects. We used intermittent method with skin pressure applied by experimental fabric at l-minute intervals. Besides we made a comparative study with results according to different restraint method (continuous method and intermittent method). As a result of this experiment, we obtained following findings. 1. The significant difference was marked at the pressure, measuring time, physique and measuring region with change of skin temperature under upper arm and thigh restraint by intermittent method. The peripheral skin temperature decreased with the lapse of restraint time. A remarkable tendency observed according to the. increase of restraint pressure. Recovery condition after release from pressure not yet recovered to original state, for all after a lapse of 10 min. The mean skin temperature decreased with the lapse of restraint time in case of upper arm restraint, it was not an obvious tendency except 60mmHg under thigh restraint. 2. Main factor affecting the evaluation of a feeling of tightness was restraint pressure, when the upper arm and thigh restraint by intermittent method. The respondence rate of 'very tight' grew larger according to the increase of restraint pressure. The value of pressure sensation declined after restraint ten times as compared with one time, but there was difference according to restraint pressure. 3. We reexamined change of skin temperature and feeling of tightness by different restraint method(continuous method and intermittent method). The results were as follows. 1) The skin temperature decreased more greatly during skin pressure by continuous method than intermittent method, especially in the peripheral. Without different restraint method, the skin temperature of slender plysique decreased more greatly than that of plump physique. 2) The value of pressure sensation by intermittent method was highly on both sites of upper arm and thigh.