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An Investigation of the Hazards Associated with Cucumber and Hot Pepper Cultivation Areas to Establish a Good Agricultural Practices (GAP) Model (오이와 고추생산 환경에서의 GAP 모델 개발을 위한 위해요소 조사)

  • Shim, Won-Bo;Lee, Chae-Won;Jeong, Myeong-Jin;Kim, Jeong-Sook;Ryu, Jae-Gee;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.46 no.1
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    • pp.108-114
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    • 2014
  • To analyze the hazards associated with cucumber and hot pepper cultivation areas, a total of 72 samples were obtained and tested to detect the presence of biological (sanitary indicative, pathogenic bacteria and fungi) and chemical hazards (heavy metals and pesticide residues). The levels of sanitary indicative bacteria (aerobic plate counts and coliforms) and fungi were ND-7.2 and ND-4.8 log CFU/(g, mL, hand, or $100cm^2$) in cucumber cultivation areas, and ND-6.8 and 0.4-5.3 log CFU/(g, mL, hand, or $100cm^2$) in hot pepper cultivation areas. More specifically, the soil of hot pepper cultivation areas was contaminated with coliforms at a maximum level of 5.6 log CFU/g. Staphylococcus aureus was detected only in glove samples at a level of 1.4 log CFU/$100cm^2$ and Bacillus cereus was detected in the majority of samples at a level of ND-4.8 log CFU/(g, mL, hand, or $100cm^2$). Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp. were not detected. Heavy metal (Zn, Cu, Ni, Pb, and Hg) chemical hazards were detected at levels lower than the regulation limit. Residual insecticides were not detected in cucumbers; however, hexaconazole was detected at a level of 0.016 mg/kg (maximum residue limit: 0.3 mg/kg) in hot peppers.

Cytotoxic Effects of Decursin from Angelica gigas Nakai in Human Cancer Cells (당귀로부터 정제한 Decursin의 인체암세포주에 대한 세포독성)

  • Park, Kyung-Wuk;Choi, Sa-Ra;Shon, Mi-Yae;Jeong, Il-Yun;Kang, Kap-Suk;Lee, Sung-Tae;Shim, Ki-Hwan;Seo, Kwon-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.11
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    • pp.1385-1390
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    • 2007
  • Anticarcinogenic-active compound was isolated and purified from Angelica gigas Nakai. The compound was identified as decursin ($C_{19}H_{20}O_5$; molecular weight 328) by mass, IR spectrophotometry $^1H-NMR$ and $^{13}C-NMR$. The proliferation decreased in a dose dependant fashion in the MCF-7 cells treated with decursin for 24 hours over the concentration of $20{\mu}g/mL$. The $IC_{50}$ value of the decursin treatment for 24 hours were 31.04, 33.60, 27,24, $20.45{\mu}g/mL$ in the SW480, 293, HepG2 and MCF-7 cells, respectively, The growth inhibitory effect was stronger in the MCF-7 cells compared to other cells including 293 of human normal cells. The chromatin condensation, apoptotic body formation and DNA fragmentation were examined in the cells treated with decursin. These results suggest that decursin from Angelica gigas Nakai inhibited the growth through apoptosis in MCF-7 cells.

Effect of pH and Temperature on the Adsorption of Heavy Metals in Acid Mine Drainage (AMD) Onto Coal Mine Drainage Sludge (CMDS) (탄광슬러지를 이용한 금속광산 산성배수 처리 시 pH및 온도의 영향)

  • Cui, Ming-Can;Lim, Jung-Hyun;Kweon, Bo-Youn;Jang, Min;Shim, Yon-Sik;Khim, Jee-Hyeong
    • Journal of Soil and Groundwater Environment
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    • v.14 no.1
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    • pp.29-35
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    • 2009
  • In this study, the effect of pH and temperature on the adsorption behavior of acid mine drainage (AMD) on coal mine drainage sludge (CMDS) has been investigated during the treatment of coal mine drainage (CMD) by electrical purification method. The pH$_{zero\;point\;charge}$ (pH$_{zpc}$) of CMDS was 5. The removal ratio of copper, zinc, cadmium, iron were increased according to the increase of pH value. The adsorption amount of copper showed 0.64 mg g$^{-1}$ sludge. It was independent of pH value. The adsorption amount of the other metals showed l.l times when pH was 3. The adsorption amount of chromium was a little bit increased at the pH value higher than 7 due to a small amount of the chromium was eluted as $Cr(OH)_6^{3-}$. The amount of metals' absorption were decreased according to temperature was increase at pH value was 3. The selectivity order was Cd>Fe > Zn > Cu. The amount of absorption showed q$_{max}$ Cu 2.747 mg g$^{-1}$ andZn 2.525 mg g$^{-1}$ when pH value higher than 5. It was independent of temperature.

Isolation and Identification of Low Molecular Weight Compounds Produced by Bacillus subtilis HJ927 and Their Biocontrol Effect on the Late Blight of Pepper (Capsicum annuum L.) (Bacillus subtilis HJ927에 의해 생성된 화합물의 분리, 동정 및 고추(Capsicum annum L.) 역병방제 효과)

  • Lee, Hyun-Jin;Park, Keun-Hyung;Shim, Jae-Han;Park, Ro-Dong;Kim, Yong-Woong;Hwang-Bo, Hoon;Cho, Jeung-Yong;Kim, Young-Cheol;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.1
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    • pp.25-31
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    • 2005
  • A soil bacterium, Bacillus subtilis HJ927, exhibiting strong antagonistic property against pathogenic fungi was isolated from pepper fields infested with Phytophthora capsici. Pepper plants inoculated with P. capsici revealed severe root mortality while plants co-inoculated with B. subtilis HJ927 and P. capsici showed drastically reduced root mortality. Low molecular weight substances released by B. subtilis HJ927 mediated the plant protective effect. The anti-fungal compounds released by B. subtilis HJ927 were identified as 3-methylbutyric acid, 2-methylbutyric acid, and methyl 2-hydroxy, 3-phenylpropanoate by high-performance liquid chromatography and gas chromatography-mass spectrometry. In addition to these compounds, B. subtilis HJ927 also produced ${\beta}$-1,3-glucanase, a hydrolytic enzyme implicated in antifungal activity.

Primary Productivity and Nitrogenous Nutrient Dynamics in the East Sea of Korea (한국 동해의 기초생산력과 질소계 영양염의 동적관계)

  • Chung, Chang Soo;Shim, Jae Hyung;Park, Yong Ghul;Park, Sang-Gap
    • 한국해양학회지
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    • v.24 no.1
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    • pp.52-61
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    • 1989
  • The daily net primary production by phytoplankton and ammonium excretion by macrozooplankton (> $350{\mu}m$) were measured to understand the nitrogenous nutrient dynamics in the southern part of the East Sea of Korea. At most of the staions, water columns were well stratified and strongly developed pycnoclines and matching nutriclines could be found near the 20-60m. Total chlorophyll ranged between $1.22-3.24{\mu}g$ ChI/l and nano-fractions of chlorophyll ranged from 43.2 to 99.6% in the surface layer. The daily net primary production by phytoplankton ranged from 0.75 to 2.04 gC/$m^2$/d and averaged to be 1.5 gC/$m^2$/d. 1t is evidenced that the primary production and chlorophyll content are relatively high in frontal waters where the North Korean Cold Water meets with the East Korean Warm Water. The turnover time of nitrate in the euphotic zone ranged from 0.2 day to 1.6 day and averaged to be 0.8 day. The N:P ratio of the study area shows on the average 13.4 which indicates nitrogenous nutrient to be the limiting factor for phytoplankton growth. Ammonium excretion by macrowoplankton averaged out to 1.3mg at-N/$m^2$/d, and contributed 7.3% of daily total nitrogen requirement by phytoplankton in this area. Calculation of upward flux of nitrate to the surface mixed layer from the lower layer approximates 7% of nitrogen requirement by phytoplankton.

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The Electrocatalytic Reduction of Molecular Oxygen with Clay Modified Electrodes (점토광물을 이용한 산소환원의 전기화학적 촉매성에 관한 연구)

  • Oh Sung-Hun;Hwang Jin-Yeon;Shim Yoon-Bo;Lee Hyomin;Yoon Jihae
    • Journal of the Mineralogical Society of Korea
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    • v.18 no.1
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    • pp.1-9
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    • 2005
  • The electrocatalytic reduction of O₂ was investigated with methyl viologen and methylene blue incorporated clay-modified electrodes. Clay suspensions were prepared with Na-montmorillonite, Ca-montmorillonite, and kaolinite. The methyl viologen-clay modified electrodes were made by coating clay suspensions adsorbing methyl viologen on a glassy carbon electrode. Cyclic voltammetry were performed in aqueous media to investigate the electrocatalytic property of the modified electrode in reducing O₂. A Na-montmorillonite modified electrode showed the greatest adsorption capacity for methyl viologen. The modified electrode made of Na-montmorillonite suspension of 0.87 g/10 mL and a 2.5 mM of methyl viologen solution showed the most effective electrocatalytic property, where the catalytic reduction potential was shifted by 242.6 mV toward the positive potential. The electrocatalytic ability was more significant in acidic (pH=3.7) and alkaline (pH=12.7) media than the neutral pH range (6.3∼8.3). The methyl viologen-Na-montmorillonite modified electrode had the good reproducibility and maintain the electrocatalytic property over 20 times reuse.

Application of major plant nutrient releasing model and N2O emissions to the leachate from the mixtures of rice hull biochar and organic fertilizer materials (왕겨 바이오차와 유기농자재 혼합에 따른 주요 양분 용출 모델 적용 및 N2O 배출량 산정)

  • DongKeon Lee;JaeLee Choi;ChangKi Shim;JooHee Nam;SeokIn Youn;JeongSeok Song;Dogyun Park;JoungDu Shin
    • Journal of the Korea Organic Resources Recycling Association
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    • v.31 no.3
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    • pp.43-53
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    • 2023
  • This batch experiment evaluated the impacts of major plant nutrient releases by applying the modified Hyperbola model on the leachates and N2O emissions from incorporated rice hull biochar with organic fertilizer materials. The treatments consisted of the control as incorporated with organic fertilizer materials, the incorporated rice hull biochar with organic fertilizer materials, and the incorporated plasma-activated rice hull biochar with organic fertilizer materials under redox conditions. The results indicated that the maximum release amount of NH4-N was 3486.3 mg L-1 in the control, and their reduction rates of NH4-N, NO3-N, PO4-P, and K were 8.0%, 17.5% 44.3.0% and 8.7%, respectively, relative to the control. In the control, the highest soluble amount of PO4-P was 681.0 mg L-1. The estimations for accumulated NH4-N, NO3-N, PO4-P, and K-releases in all the treatments were significantly (p<0.01) fitted with a modified Hyperbola model. For greenhouse gas emissions, the lowest cumulative N2O was 340.4 mg kg-1 in the soil incorporated with plasma-activated rice hull biochar, and the reduction rates were 27.8% and 86.4% in the rice hull biochar and plasma-activated rice hull biochar treatments, respectively, compared to the control. Therefore, it concluded that the incorporated rice hull biochar can be especially useful for controlling PO4-P release and N2O emissions for bio-fertilizer applications.

Quality Characteristics of Wet Noodle Added with Freeze Dried Garlic Powder (동결건조 마늘 분말을 첨가한 생면의 품질 특성)

  • Jeong, Chang-Ho;Shim, Ki-Hwan;Bae, Young-Il;Choi, Jine-Shang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.10
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    • pp.1369-1374
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    • 2008
  • This study investigated proximate compositions and quality characteristics of wet noodles with the addition of freeze dried garlic powders prepared at 0.5, 1.0, 2.0, and 4.0% based on flour source, respectively. The moisture, crude protein, crude fat, nitrogen free extract, crude fiber, and crude ash of the freeze dried garlic powder were $4.72{\pm}0.14%,\;19.46{\pm}0.28%,\;1.21{\pm}0.10%,\;68.45{\pm}2.06%,\;2.38{\pm}0.05%,\;and\;3.78{\pm}0.06%$, respectively. The cooked weight, volume, water absorption of cooked noodles were decreased, but the turbidity of soup was increased with the addition of garlic powder. Increased of L (lightness), a (redness) and b (yellowness) values were caused as the amount of garlic powder increased in dough. Decrease of L value and increase of a and b value were shown with the increase of garlic powder in cooked noodle. The cooked noodles with garlic powder were significantly lower in hardness, adhesiveness, springiness chewiness, gumminess and cohesiveness than those of 100% wheat noodle. Sensory evaluation indicated that cooked noodles with 0.5% garlic powder was not significantly different from the control.

Development of an Environmental Friend Additive Using Antibacterial Natural Product for Reducing Enteric Rumen Methane Emission (항균활성 천연물질을 이용한 반추위 메탄저감용 친환경 첨가제 개발)

  • Lee, A-Leum;Yang, Jinho;Cho, Sang-Buem;Na, Chong-Sam;Shim, Kwan-Seob;Kim, Young-Hoon;Bae, Gui-Seck;Chang, Moon-Baek;Choi, Bitna;Shin, Su-Jin;Choi, Nag-Jin
    • Korean Journal of Organic Agriculture
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    • v.22 no.3
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    • pp.491-502
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    • 2014
  • The present study was conducted to investigate effective starter culture to improve biological activity of Asarum sieboldii. Antibacterial activity, antioxidant activity and reduction of enteric rumen methane production were used as criterions for biological activity. Ground A. sieboldii was added in MRS broth at 10% (w/v) and fermented by different starter cultures. Weissella confusa NJ28, Weissella cibaria NJ33, Lactobacillus curvatus NJ40, Lactobacillus brevis NJ42, Lactobacillus plantarum NJ45 and Lactobacillus sakei NJ48 were used for starter culture strains. Each starter culture was inoculated with 1% (v/v) ratio and fermentation was performed at $30^{\circ}C$ with agitation (150 rpm) for 48 h. MRS broth for the control was employed without starter culture. Then the fermentation growth was dried and extracted using ethyl alcohol. The growth of starter culture was detected at NJ40, NJ42, NJ45 and NJ48. And the highest cell growth was found in NJ40. Antibacterial activity against to Staphylococcus aureus, Listeria monocytogens, Mannheimia haemolytica and Salmonella gallinarum were observed in the extract fermented by NJ40 and NJ45. All treatments showed antioxidant activities, however, there were no significant differences (p>0.05). In in vitro rumen fermentation, negative control (NC) and positive control (PC) were assigned to without extract and with non-fermented A. sieboldii extract. Significant suppression of gas productions were detected in positive control and treatments compared to negative control (p<0.05). However, total volatile fatty acid production was not suppressed. Significant methane reduction per total volatile fatty acid productions were found in positive control and NJ45 treatment (p<0.05). The present study suggested a fermentation of A. sieboldii using NJ45 strain could improve its biological activity and make possible for its use in bio additive for enteric rumen methane mitigation without suppression of animal productivity.

Investigation on Beef Quality Indicator of Off-Flavor Development during Storage (쇠고기 저장 중 이취발생에 대한 Indicator 탐색)

  • Byeon, Ko-Eun;An, Soo-Rim;Shim, Soo-Dong;Lee, Jung-Young;Hong, Kwang-Won;Min, Sang-Gi;Lee, Seung-Ju
    • Food Science of Animal Resources
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    • v.29 no.3
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    • pp.325-333
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    • 2009
  • Beef quality indicators of off-flavor development during storage were investigated in terms of temperature dependence. The off-flavor development time (ODT) was detected using the R-index sensory test. During varying storage conditions at $25^{\circ}C$, $15^{\circ}C$, and $5^{\circ}C$, elements of beef quality were measured, such as volatile basic nitrogen (VBN), pH, color (CIE $L^*$, $a^*$, $b^*$), Warner-Bratzler shear force (WBSF), Pseudomonas spp. CFU, and lactic acid bacteria (LAB) CFU. A model with temperature dependence of ODT during storage was developed using Arrhenius-like equation, and a requirement with quality indicators was mathematically derived, resulting in similar temperature dependence. The temperature dependence of beef quality indicators was represented by the Arrhenius activation energy (Ea). Upon comparing the Ea of beef quality indicators and ODT, the temperature dependence similarity was found to be higher in the order of three groups: VBN, pH, $a^*$ value; LAB, Pseudomonas spp.; and WBSF, $L^*$ value, $b^*$ value. Therefore, VBN were determined as the most effective indicator of beef quality during off-flavor development.