• YAKHAK HOEJI
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• v.60 no.3
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• pp.128-134
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• 2016

L1 cell adhesion molecule (L1CAM) is a cell surface molecule to initiate a variety of cellular responses through interacting with other cell adhesion molecules in a homophilic or heterophilic manner. Although its expression was found to be upregulated in some tumor cells, including cholangiocarcinomas, and ovarian cancers, and many studies have investigated the role of L1CAM in these cancers, its role in inflammatory responses has been poorly understood. In this study, we explored the role of L1CAM in macrophage-mediated inflammatory responses. L1CAM significantly suppressed the production of nitric oxide (NO), but induced cell proliferation in RAW264.7 cells. L1CAM expression was detectable, but its expression was markedly decreased by lipopolysaccharide (LPS) in RAW264.7 cells. In addition, the expression of pro-inflammatory genes, such as tumor necrosis factor (TNF)-${\alpha}$, cyclooxygenase (COX)-2, and inducible nitric oxide synthase (iNOS) induced by LPS was dramatically suppressed by L1CAM in RAW264.7 cells. L1CAM inhibited the transcriptional activities of NF-${\kappa}B$ and AP-1 while its cytoplasmic domain deletion form, $L1{\Delta}CD$ did not suppressed their activities in RAW264.7 cells. Moreover, L1CAM suppressed nuclear translocation of p65 and p50 as well as c-Jun, c-Fos and p-ATF2 which are transcription factors of NF-${\kappa}B$ and AP-1, respectively. In conclusion, L1CAM suppressed inflammatory responses in macrophages through inhibiting NF-${\kappa}B$ and AP-1 pathways.

• Molecules and Cells
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• v.40 no.5
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• pp.363-370
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• 2017

Extrahepatic cholangiocarcinoma (ECC), a malignant tumor of biliary origin, has a poor prognosis with limited treatment options. The KRAS oncogene is the most commonly mutated gene in ECC and one of the factors that predicts a poor prognosis and low survival rate. L1 cell adhesion molecule (L1CAM) is expressed in ECC cells and acts as an independent poor prognostic factor in predicting patient survival. In this study we investigate the functional significance of L1CAM in ECC cells with activating KRAS mutation. We selected an ECC cell line, EGI-1, with activating KRAS mutation, and then confirmed its expression of L1CAM by RT-PCR, western blot analysis, and flow cytometry. The suppression of L1CAM expression (using a specific lentivirus-delivered shRNA) significantly decreased the migratory and invasive properties of EGI-1 cells, without altering their proliferation or survival. Analyses of signaling effectors in L1CAM-depleted and control EGI-1 cells indicated that L1CAM suppression decreased the levels of both phosphorylated MKK4 and total MKK4, together with c-Jun N-terminal kinase (JNK) phosphorylation. Further, exposure to a JNK inhibitor (SP600125) decreased migration and invasion of EGI-1 cells. These results suggest that L1CAM promotes cellular migration and invasion via the induction of MKK4 expression, leading to JNK activation. Our study is the first to demonstrate a functional role for L1CAM in ECC carrying the activating KRAS mutation. Given that KRAS is the most commonly mutated oncogene in ECC, L1CAM may serve as an attractive therapeutic target for ECC cells with activating KRAS mutation.

• BMB Reports
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• v.38 no.4
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• pp.432-439
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• 2005

$Ca^{2+}$/calmodulin transduction pathways have been implicated in mediating stress response and tolerance in plants. Here, three genes encoding calmodulin (Cam) members of the EF-hand family of $Ca^{2+}$-binding proteins were identified from Oryza sativa L. databases. Complementary DNA for each of the calmodulin genes, OsCam1, OsCam2, and OsCam3 were sequenced. OsCam1 and OsCam2 encode a conventional 148-amino acid calmodulin protein that contains four characteristic $Ca^{2+}$-binding motifs. OsCam3 encode a similar protein with a 38-amino-acid extension containing a putative prenylation site (CVIL) at the carboxyl terminus. RT-PCR showed that each of the genes is expressed in leaves and roots of 2-week old rice seedlings. By RNA gel blot analysis, OsCam1 mRNA levels strongly increased in response to NaCl, mannitol and wounding treatments. In contrast, OsCam2 mRNA levels were relatively unchanged under all conditions investigated. NaCl treatment and wounding also increased the OsCam3 mRNA level, but in a more transient manner. Our results indicate that although the expression of genes encoding different calmodulin isoforms is ubiquitous, they are differentially regulated by various stress signals. In addition, we have demonstrated that the calcium-channel blocker lanthanum chloride inhibited the induction of OsCam1 gene expression by both NaCl and mannitol treatments. These results suggest that osmotic stress induced expression of OsCam1 gene requires the $[Ca^{2+}]_{cyt}$ elevation that is known to occur in response to these stimuli.

• Journal of Oriental Neuropsychiatry
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• v.33 no.4
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• pp.401-424
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• 2022

Objectives: As effective treatments for dementia are lacking in Western medicine, complementary and alternative medicine (CAM) is considered a useful option. While the quality of life (QoL) is a vital outcome for patients with dementia, the QoL of patients receiving CAM for dementia remains ambiguous. This study aimed to determine the effect of CAM on QoL outcomes in dementia patients. Methods: A search was performed using the keywords "dementia," "Alzheimer's," "cognitive impairment," "Chinese," "Korean," "oriental," "herbal," "acupuncture," and "quality of life". All quantitative data were synthesized using R version 4.1.1. Results: Twenty-five randomized controlled trials (RCTs), 16 pre-post trials, and two cohort studies were selected for the systematic review. QoL in Alzheimer's disease (QOL-AD) (n=11, 25.6%) and geriatric QoL in dementia (GQOL-D, n=9, 20.9%) were the most utilized QoL instruments. Significant benefits in QoL were observed after receiving mind, body, combined mind and body, nursing, oriental medicine, and acupuncture therapies. In the meta-analysis, the combined effect was shown to significantly increase QOL-AD compared to before CAM interventions (standardized mean difference, SMD: 0.507; 95% confidence interval (CI), 0.191~0.824; p<0.01). The overall synthesized estimates in the GQOL-D showed a significantly improved QoL (SMD: 0.537, 95% CI: 0.238~0.837 p<0.01; one group; SMD: 1.465, 95% CI: 0.934~1.996, p<0.01). The seven studies assessing the cost-effectiveness of CAM reported uncertain outcomes. Conclusions: This study showed that CAM interventions benefited patients with dementia by improving their QoL. While additional standardized research is required, CAMs are suggested as effective clinical management for patients with dementia. They are also suggested as complementing therapies for these patients.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.2 no.2
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• pp.109-114
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• 1998

The induction of crassulacean acid metabolism(CAM) characterized by day/night acid fluctuation was investigated in leaves from 10 days exposure to elevated $CO_2$ concentration(1,000 ${\mu}L\;L^{-1}CO_2).$ For Pereskia aculeata, have $C_3-like$ gas exchange pattern in well watered condition and shift into CAM-like in water stress, showed a more typical CAM-like diurnal acid fluctuation. Whereas the massive diurnal fluctuation of acidity in typical CAM of Kalanchoe rosea was declined. The effect of short-term exposure to various concentrations of $CO_2$ on titratable acidity in P. aculeata and K. rosea was also investigated. To investigate the response of various $CO_2$ concentrations, four different $CO_2$ levels(350, 700, 1,000 and 1,500 ${\mu}L\;L^{-1})$ were imposed for 24hr and measured the titratable acidity at 06:00, when the acidity was maximum, and 14:00, when the acidity was minimum. The accumulation of acid in P. aculeata was enhanced markedly by higher concentration of $CO_2,$ while the level of acidity in f rosea did not highly respond to $CO_2.$ A notable difference between P. aculeata and K. rosea was the response of de-acidification to a higher concentration of $CO_2$ Increasing with $CO_2,$ the degree of do-acidification of P. auleata was increased while that of K. rosea was depressed.

• Asian Journal of Turfgrass Science
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• v.10 no.2
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• pp.125-142
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• 1996

Crassulacean acid metaholism (CAM) was investigated in leaves and stems of the succulent $C_4$dicot Portulaca oleracea L. Under 14-hour days, stem tissues showed much greater fluctuation of acidity than leaf tissues. But leaf and stem tissues showed almost same CAM-like pattern of acid fluctuation under 8-hour days. Stem tissues of R oleracea grown under the naturai environment showed high CAM activity, but no CAM activity was seen in leaves of those plants. In the naturally growing plants, the rapid acidification was seen in intact stems at dawn, but defoliated stems showed only a gradual increase. RuBP carlboxylase activity was very high at 2:00 P.M. in both leaves and stems. However, its activity at 1:00 A.M. and 5:30 AM. was hardly detected. particularly, activity of PEP carboxylase in leaves was very high in the early morning, though that in stem tissues was little. These results indicate that $CO_2$ passed through open stomata at dawn may be assimilated by PEP carboxylase in leaves, and then $C_4$ products move to stems. The levels of nitrate concentration and of nitrate reductase were higher in stems than in leaves. The levels were also higher in the light than in the dark. It would be suggested that considerable amount of nitrate absorbed from roots ho assimilated in stems, and nitrate transferred to leaves via stem tissues be reduced there. Key words: Portalaca oleracea, Cactus, Photosynthesis, Nitrogen assimilation, Crassulacean acid metabolism (CAM).

• Journal of Astronomy and Space Sciences
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• v.9 no.1
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• pp.41-51
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• 1992

Photoelectric observations of close binary stars, AO Cam and AW Cam, were made during the 1984 observing season with the 61cm reflector at the Sobaeksan Observatory. One time of primary minimum for AO Cam and three primary epochs for AW Cam were derived from the observations of these two systems. Times of minimum light of these two binaries collected from literature were analyzed with a least square fitting method. New improved light elements for AO Cam and AW Cam were determined. The orbital period of AO Cam had been constant from Octobar, 1980 (JD 244520) to February, 1985 (JD 2446107). However, one secondary time of minimum (JD 2447864.7879) of AO Cam published recently by Mullis and Faulkner(1991) shows large deviation of about 4.6 minutes ($0^d.0032$) from the one predicted by our new light elements. Future observations of times of minima for this system are needed to test this period change. The orbital period of AW Cam has been constant as P=$0^d.77134645$ for about sixty years from the early 1930's to the present.

• Journal of Korean society of Dental Hygiene
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• v.16 no.5
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• pp.695-700
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• 2016

Objectives: The purpose of the study was to investigate the effect of CAD/CAM ceramic thickness on shade. Methods: 24 disk-shaped ceramic specimens (E.Max CAD & Empress CAD, $12mm{\times}12mm$) were prepared and divided into 4 groups (n=6). Ceramic specimens in 2 thicknesses (0.8 mm, 1.0 mm) were made low translucency(LT) shade A2. All specimens were measured as L*, a*, b* using spectrophotometer. The color differences(${\Delta}E$ values) between the specimen and a control target block ($12{\times}12{\times}1.2mm$) were calculated. Two-way ANOVA performed between 2 groups(material and thickness). Results: As the thickness increased, the $L^*$ (for all groups) and $b^*$ value (for all groups) increased and the $a^*$ value(for LR group) decreased. The mean color differences caused by thickness was acceptible (${\Delta}E$ > 2) for group LD1. But the mean color differences by thickness were not selected (${\Delta}E$ < 2) for group LD2, LR1, LR2. Conclusions: The different thickness can influence the final color of CAD/CAM ceramic.

• BMB Reports
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• v.41 no.11
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• pp.771-777
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• 2008

Calmodulin (CaM) proteins, members of the EF-hand family of $Ca^{2+}$-binding proteins, represent important relays in plant calcium signals. Here, OsCam1-1 was isolated by PCR amplification from the rice genome. The gene contains an ORF of 450 base pairs with a single intron at the same position found in other plant Cam genes. A promoter region with a TATA box at position-26 was predicted and fused to a gus reporter gene, and this construct was used to produce transgenic rice by Agrobacterium-mediated transformation. GUS activity was observed in all organs examined and throughout tissues in cross-sections, but activity was strongest in the vascular bundles of leaves and the vascular cylinders of roots. To examine the properties of OsCaM1-1, the encoding cDNA was expressed in Escherichia coli. The electrophoretic mobility shift when incubated with $Ca^{2+}$ indicates that recombinant OsCaM1-1 is a functional $Ca^{2+}$-binding protein. In addition, OsCaM1-1 bound the CaMKII target peptide confirming its likely functionality as a calmodulin.

• Proceedings of the Korean Information Science Society Conference
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• 2004.10c
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• pp.124-126
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• 2004

본 논문은 CAM(Constant Awake Mode), 및 PSM(Power Saving Mode)을 지원하는 IEEE 802.l1b[1] Infrastructure 환경에서 전송 프로토콜이 이동기기의 WNIC(Wireless Network Interface Card) 에너지 소비에 미치는 영향을 분석하고 단위 시간 동안 전송되는 데이터 양에 기반한 IEEE 802.l1b WNIC 에너지 모델을 제안한다. 제안하는 에너지 모델은 IEEE 802.l1b 환경에서 CAM 및 PSM 모드를 사용할때, 실제 측정한 값을 기반으로 설계되었다. 제안된 에너지 모델은 실험을 통하여 정확성을 검증하고, IEEE 802.l1b를 기반으로한 저전력 통신 관련 연구에서 시뮬레이션 시 사용 가능한 에너지 모델임을 밝힌다.