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Optimal Timing to Assess Drain Amylase Concentration after Elective Gastrectomy

  • Wakahara, Tomoyuki;Kanemitsu, Kiyonori;Miura, Susumu;Tsuchida, Shinobu;Iwasaki, Takeshi;Sasako, Mitsuru
    • Journal of Gastric Cancer
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    • v.21 no.1
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    • pp.30-37
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    • 2021
  • Purpose: While the amylase concentration of the drainage fluid (dAmy) has been reported to be a predictor of postoperative pancreas-related complications (PPRC), the optimal timing for its measurement has not been fully investigated. Materials and Methods: The clinicopathological data of 387 patients who underwent elective gastrectomy for gastric cancer were reviewed. Laboratory data, including dAmy on postoperative days 1 (dAmy1) and 3 (dAmy3), and serum C-reactive protein (sCRP) concentrations on postoperative days 1 (sCRP1) and 3 (sCRP3) were compared between patients with PPRC and without PPRC. Results: Nineteen of the 387 patients (4.9%) developed PPRC. The optimal cutoff values of dAmy1, dAmy3, sCRP1, and sCRP3 were 1514 IU/L, 761 IU/L, 8.32 mg/dL, and 15.15 mg/dL, respectively. The area under the curve of dAmy1 was greater than that of dAmy3 (0.915 vs. 0.826), and that of sCRP3 was greater than that of sCRP1 (0.820 vs. 0.659). In the multivariate analysis, dAmy1 (P<0.001) and sCRP3 (P=0.004) were significant predictors of PPRC, while dAmy3 (P=0.069) and sCRP1 (P=0.831) were not. Thirteen (41.9%) of 31 patients with both dAmy1 ≥1,545 IU/L and sCRP3 ≥15.15 mg/dL had PPRC ≥Clavien-Dindo II. In contrast, among 260 patients with both dAmy1 <1,545 IU/L and sCRP3 <15.15 mg/dL, none developed PPRC. Conclusions: dAmy1 was more useful than dAmy3 in predicting PPRC. The combination of dAmy1 and sCRP3 may be a useful criterion for the removal of drains on postoperative day 3.

Quality characteristics and antioxidant activity of drink prepared with black garlic and Oenanthe javanica DC (흑마늘과 미나리를 이용하여 제조한 음료의 품질 및 항산화 특성)

  • Jeong, Tae-Seong;Kim, Jin-Hak;An, Sin-Ae;Won, Yong-Duk;Lee, Shin-Ho
    • Food Science and Preservation
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    • v.21 no.2
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    • pp.193-198
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    • 2014
  • The quality and antioxidative characteristics of drinks prepared with different mixing ratios of black garlic and Oenanthe javanica DC., BD-1 (black garlic only), BD-2 (black garlic:Oenanthe javanica DC.=2:1), BD-3 (black garlic:Oenanthe javanica DC.=1:1), and BD-4 (black garlic:Oenanthe javanica DC.=1:2), were studied. The pH increased with the increasing concentration of Oenanthe javanica DC. extract in all the tested drinks, but the sugar contents decreased. The total polyphenol contents of the drinks were 28.48 ${\mu}g/mL$ (BD-1), 41.91 ${\mu}g/mL$ (BD-2), 42.36 ${\mu}g/mL$ (BD-3), and 46.96 ${\mu}g/mL$ (BD-4). The SOD-like activity was highest for BD-4 (18.60%), followed by BD-3 (15.53%), BD-2 (12.53%), and BD-1 (10.27%). The thiobarbituric acid reactive substances (TBARS) was highest for BD-4 (52.51%), followed by BD-3 (45.70%), BD-2 (39.44%), and BD-1 (28.72%). The ferrous ion chelating activity increased with the increasing concentration of Oenanthe javanica DC extract, and BD-4 showed the best activities among all the tested drinks. The water-soluble vitamin content (vitamins B1, B2, B6, and C) of BD-4 (1197.77 ${\mu}g/mL$) was higher than those of the other drinks (BD-1, 213.02 ${\mu}g/mL$; BD-2, 477.87 ${\mu}g/mL$; BD-3, 914.72 ${\mu}g/mL$), and the vitamin C (806.21 ${\mu}g/mL$) content of the water-soluble vitamins at BD-4 was higher than those of vitamins B1 (68.04 ${\mu}g/mL$), B2 (312.51 ${\mu}g/mL$), and B6 (11.01 ${\mu}g/mL$). BD-4 showed the best score in the sensory evaluations, such as in the evaluation of the color, flavor, taste, and overall acceptability.

Water Quality Variation and Biotic Community Characteristics in Juam Lake (2011) (주암호의 수질 변동 및 생물군집 특성(2011))

  • Song, Hyo-Jeong;Hwang, Kyung-Sub;Park, Jong-Hwan;Lee, Hak-Young;Kim, Jong-Sun;Kim, Hyun-Woo;Lim, Dong-Ok;Lee, Sung-Hwi;Lim, Byung-Jin
    • Korean Journal of Environmental Biology
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    • v.31 no.1
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    • pp.37-44
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    • 2013
  • This study was to investigate water quality and biotic community characteristics in Juam Lake. In water quality, water temperatures was $3.8{\sim}21.2^{\circ}C$, 6.7~8.6 in pH, $64{\sim}76{\mu}s\;cm^{-1}$ in Conductivity, $5.3{\sim}13.2mg\;L^{-1}$ in DO, $2.5{\sim}3.3mg\;L^{-1}$ in COD, $1.0{\sim}5.1mg\;L^{-1}$ in SS, $0.622{\sim}0.841mg\;L^{-1}$ in T-N, $0.007{\sim}0.019mg\;L^{-1}$ in T-P and $2.8{\sim}8.8mg\;m^{-3}$ in Chl-a. Revised Carlson's Index (TSIm) assessment using total phosphorus (TP) and chlorophyll-a domonstrated that the trophic states of Juam Lake were rated as mesotrophic. A total of 53 species of phytoplankton were identified. They were 28 Bacillariophyceae, 13 Chlorophyceae, 3 Cyanophyceae, and 9 Other algal taxa. The standing crops of phytoplankton was ranged from $113cells\;mL^{-1}$ to $2,909cells\;mL^{-1}$. A total of 16 species of zooplankton were identified (10 rotifers, 4 cladocerans and 2 copepods). Total zooplankton abundance was $309ind.\;L^{-1}$ to $435ind.\;L^{-1}$. The collected benthic macroinvertebrates from the surveyed sites in Juam Lake were 1,038 individuals, 33 species, 21 families and 12 orders. A dominant species was Uracanthella rufa and a subdominant species was Ecdyonurus kibunensis. Hydrophytes recorded from Juam Lake were identified 9 taxa. Emerged plants, floating plants among the hydrophytes was classified 8, 1 taxa, respectively. Ecosystem disturbance wildplant by Environment Ministry found were Paspalum distichum var. distichum and Ambrosia artemisiaefolia. A total of 30 species (6 families) were collected fishs from Juam Lake. There were 10 Korean endemic species (33.3% of collected species number) and 3 exotic species (10.0%).

Antioxidant activity of solvent extracts from Vitis coignetiea skins (용매별 머루 과피 추출물의 항산화 효과)

  • Park, Hyun-Sil
    • Culinary science and hospitality research
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    • v.17 no.1
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    • pp.208-217
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    • 2011
  • This study explores the antioxidant activity of solvent extracts from Vitis coignetiea skins, and the result is as follows. Total phenolic compound and flavonoids contents were the highest in ethyl acetate extract, $53.4{\pm}1.2\;mg$/100 g and $644.7{\pm}3.7\;mg$/100 g, respectively. Electron donating abilities of solvent extracts from Vitis coignetiea skin were proportionally increased with concentration and ethyl acetate extract ($80.93{\pm}0.58%$) showed the higher activity than BHT($63.21{\pm}0.72%$) at concentration of $50\;{\mu}l$/mL. Reducing power of solvent extracts from Vitis coignetiea skin was the highest in ethyl acetate extract(1.769) at concentration of $1,000\;{\mu}l$/mL. TBARS of ethyl acetate extract was higher than ascorbic acid. Nitrite scavenging ability of solvent extracts from Vitis coignetiea skin(pH 1.2, $1,000\;{\mu}l$/mL) was the highest in ethyl acetate extract($92.5{\pm}2.4%$).

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Melanogenic Effect of Eclipta Prostrata (L.) L. (한련초의 멜라닌합성 촉진 효과)

  • Cha, Su Bin;Park, In Hae;Hong, Seok Hun;Mun, Yeun Ja;Woo, Won Hong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.30 no.6
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    • pp.432-438
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    • 2016
  • This study was peformed to investigate the mechanism of ethanol extract of Eclipta Prostrata (L.) L. (EEP) induced melanogenesis. EEP enhanced tyrosinase activity and melanin contents of B16F10 cells. Moreover, EEP increased the protein expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). But EEP did not increase the protein expression of tyrosinase-related protein 2 (TRP-2). These results suggest that melanogenesis-promoting effect of EEP was involved in regulation of tyrosinaase and TRP-1 protein, and EEP may be a potent pigmentation darkening agent in hypopigmentation condition.

Pharmacokinetics of Procainamide and N-acetylprocainamide (Procainamide와 그 대사산물(N-acetylprocainamide)의 약동학적 분석에 관한 연구)

  • Chang, In-Jin;Shin, Jae-Gook;Shin, Sang-Goo;Park, Chan-Woong;Lim, Jung-Kyoo
    • The Korean Journal of Pharmacology
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    • v.25 no.1
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    • pp.101-107
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    • 1989
  • To evaluate disposition characteristics of procainamide and its active metabolite, N-acetylprocainamide (NAPA), cross-over study for procainamide and NAPA was performed in 5 male adult dogs. After single administration of 10 mg/kg procainamide over 15 minutes, the range of measured plasma NAPA concentrations during experimental period were 0.03 to 0.124 ug/ml and early 'dip' phenomenon was distinct on NAPA concentration to time curve in all 5 dogs. Volume of distribution (Vss) and central compartment volume (Vc) of procainamide were $1.20{\pm}0.27\;L/kg$ of body weight and $0.36{\pm}0.08\;L/kg$, respectively. Vss and Vc of NAPA were $1.21{\pm}0.21\;L/kg$ and $0.26{\pm}0.07\;L/kg$, respectively. Intercompartmental clearance (Clint) of procainamide was 3.44 L/kg/hr and that of NAPA was 1.62 L/kg/hr. Total body clearance (Cl) of procainamide and NAPA were $0.47{\pm}0.08$ and $0.35{\pm}0.08\;L/kg/hr$. The half-life $(t_{1/2{\beta}})$ of procainamide and NAPA were 2.85 hrs and 2.77 hrs, respectively. Metabolic clearance (Clm)of procainamide by N-acetylation was $18.24{\pm}6.22\;ml/kg/hr$, which corresponded to 3.9% of total procainamide clearance.

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Effect of Jasmonic Acid on the Root Growth and the Eleutheroside Accumulation in the Adventitious Root Culture of Eleutherococcus senticosus (가시오갈피나무 부정근(不定根) 배양(培養)에서 부정근(不定根)의 생장(生長)과 Eleutheroside유(類)의 생산(生産)에 미치는 Jasmonic acid처리(處理)의 영향)

  • Ahn, Jin-Kwon;Lee, Wi-Young;Park, Eung-Jun;Park, Young-Ki
    • Journal of Korean Society of Forest Science
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    • v.97 no.3
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    • pp.249-254
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    • 2008
  • This study was carried out to investigate the dose-dependent effect of jasmonic acid on both the adventitious root growth and the accumulation of various eleutherosides in the Eleutherococcus senticosus bioreactor culture. The highest biomass production (4.6 g DW/L) was observed in the absence of jasmonic acid and the root growth was significantly decreased by increasing the jasmonic acid concentration. However, jasmonic acid stimulated the production of both eleutheroside B, E and $E_1$. The highest level of eleutheroside B and E ($476.3{\mu}g/g\;DW$ and $676.0{\mu}g/g\;DW$) was obtained by 0.5 mg/L jasmonic acid treatment, while eleutheroside $E_1$ was accumulated at the highest level by 0.01 mg/L jasmonic acid treatment. The highest content of total eleutheroside was $2468.6{\mu}g/L$ when 0.01 mg/L jasmonic acid was applied. In addition, when the adventitious roots were cultured with 0.01 mg/L jasmonic acid, the highest levels of eleutheroside B, E and $E_1$ were observed at the 6th, 8th and 4th day of culture, respectively.

Isolation and Identification of Lactic Acid Bacteria from Commercial Kimchi (시판김치로부터 젖산균의 분리 및 동정)

  • Ko, Jung-Lim;Oh, Chang-Kyung;Oh, Myung-Cheol;Kim, Soo-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.6
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    • pp.732-741
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    • 2009
  • This study was carried out to identify lactic acid bacteria isolated from commercial Kimchi. Twelve lactic acid bacteria strains were isolated from Chinese cabbage kimchi (Baechu kimchi) that was fermented for 4 days at room temperature after making kimchi, 6 strains from pickled ponytail radishes (Chongkak kimchi) that was fermented for 2 days, and 15 strains in radish cube kimchi (Kaktugi) that was fermented for 5 days, and 23 strains were isolated in pickled Wakegi (Pa kimchi) that was fermented for 4 days. Eight strains among the lactic acid bacteria of 12 strains isolated from Baechu kimchi (pH 4.0) were identified as Lactobacillus plantarum, 1 strain as Leuconostoc lactis, 2 strains as Lactobacillus casei subsp. pseudoplantarum, and 1 strain as Lactobacillus sake. Three strains among the lactic acid bacteria of 6 strains isolated from Chongkak kimchi (pH 4.5) were identified as Leuconostoc paramesenteroides, 2 strains as Leuconostoc mesenteroides subsp. mesenteroides, and 1 strain as Lactobacillus plantarum. Two strains among the 15 strains isolated in Kaktugi (pH 4.0) were identified as Leuconostoc lactis, 3 strains as Leuconostoc mesenteroides subsp dextranicum, 4 strains as Lactobacillus casei subsp. pseudoplantarum, and 4 strains as Lactobacillus coryniformis subsp. torquens. Twenty-two strains among the 23 strains isolated from Pa kimchi (pH 4.1) identified as L. plantarum and 1 strain was as Lactobacillus sake. From the results above, the dominant species of Baechu kimchi was confirmed as L. plantarum, Chongkak kimchi as L. paramesenteroides, Kaktugi as L. casei subsp. pseudoplantarum and L. coryniformis subsp. torquens, and Pa kimchi as L. plantarum.

Evaluation of the Microbiological Safety for the Seawater in a Part of the South Coast, Korea (남해안 일부해역 해수의 세균학적 위생안전성 평가)

  • Park, Yeong Min;Yoon, Hyun Jin;Ham, In Tae;Yoo, Hean Jae;Choi, Jong-Duck
    • Journal of Food Hygiene and Safety
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    • v.30 no.4
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    • pp.350-358
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    • 2015
  • This study was designed to evaluate the sanitary characteristics of sea water in a part of the south coast, Korea and to check the seawater which is in compliance with the recommended bacteriological criteria for shellfish cultivation. The samples of sea water were collected at 14 sampling stations established in the survey area between March 2014 and October 2014. Food poisoning caused by seafood consumption is often associated with pathogenic microorganisms originated from fecal contamination. Therefore, fecal coliform is very important criteria for evaluating the safety of fisheries in coastal areas. The range of geometric mean (GM) and the estimated 90th percentile values of total coliform were 4.1~83.1 MPN/100 mL, and 11.7~834.1 MPN/100 mL, respectively. The GM and the estimated 90th percentile values of fecal coliform were 2.5~22.7 MPN/100 mL and 2.5~170.0 MPN/100 mL, respectively. Therefore, the bacteriological safety of seawater at this shellfish-growing area met with the Korean Shellfish Sanitation Program (KSSP) criteria for a growing area. However, the values are seasonally exceed the KSSP criteria, suggesting that the monitoring and evaluation of seawater quality is very important in shellfish-growing area.

Stabilization of Residual Heavy Metals after Soil Washing of Mine Tailings Contaminated with Arsenic and Heavy Metals (비소와 중금속으로 오염된 광미의 토양세척 후 잔류 중금속의 안정화 처리)

  • Lim, Mihee;Kim, Myoung-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.1
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    • pp.67-73
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    • 2014
  • In this study, the residual heavy metals in the mine tailings, primarily treated by soil washing, were stabilized using phosphate salts. The concentrations of residual contaminants in the washed mine tailings were As (1,861 mg/kg), Cd (20 mg/kg), Cu (56 mg/kg), Pb (2,149 mg/kg), and Zn (633 mg/kg). They were stabilized with $CaHPO_4$, $Ca(H_2PO_4)_2{\cdot}H_2O$, and hydroxyapatite at 0.1 wt%, 1 wt%, and 10 wt% for 1, 3, 5, 7, and 14 days. It was found that 1 wt% $Ca(H_2PO_4)_2{\cdot}H_2O$ was optimum in our experiments, but the stabilization duration did not affect the efficiency. After stabilization with 1 wt% $Ca(H_2PO_4)_2{\cdot}H_2O$ for 1 day, the concentrations of arsenic and heavy metals, As (0.328 mg/L), Cd (0.250 mg/L), Cu (0.143 mg/L), Pb (0.359 mg/L), and Zn (2.622 mg/L), in TCLP leachate were below the RCRA-TCLP limits, which meant the contaminants in the treated mine tailings were stably immobilized.