• Food Science and Preservation
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• v.31 no.1
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• pp.161-172
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• 2024

This study investigated the anti-obesity effects of lactic acid bacteria ferments cultured in industrial medium with ethanol extract of ramie leaf (Boehmeria nivea L.). On the 4th day of fermentation, the maximum live cell counts were 8.75-8.85 log CFU/mL, pH was 3.74-3.79, and total acidity was 2.07-2.19%. The fermentation of lactic acid bacteria on the fourth day resulted in the amount of lactic acid reaching 1,676.03-1,910.12 mg%. The lipase inhibitory activities of Lactiplantibacillus plantarum (L. plantarum) JBLAB0101 (FRLPLA) and Lactobacillus rhamnosus GG (LGG, (FRLLGG)) ferments were 30.10%, and 25.63%, respectively, at a concentration of 0.5 mg/mL. The lipid accumulation, leptin production, PPAR-γ and SREBP-1c mRNA levels were decreased to 37.54%, 54.64%, 24.18%, and 31.32%, respectively, at 200 ㎍/mL concentration of FRLPLA. These results suggest that anti-obesity effect could be increased by lactic acid bacteria in industrial medium with extract of ramie leaf.

• Korean Journal of Veterinary Service
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• v.21 no.1
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• pp.87-95
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• 1998

The present study was conducted to characterize maternal antibody status which haemagglutination inhibition(HI) titers against canine parvovirus(CPV) in the 15 puppies delivered from 3 dams. The range of HI titers of 5 puppies delivered from a mother dog(A) with HI titer of 1 : 1,024 were 1 : 16~1 : 64 at 1 day old before suckling, 1 : 512~1 : 1,024 at 2 days old after suckling, 1 : 512~1 : 2,048 at 1 week old, 1 : 256~l : 1,024 at 2 weeks old, 1 : 128~l : 512 at 3 weeks old, 1 : 128~l : 256 at 4 weeks old, 1 : 32~1 : 128 at 5 weeks old, 1 : 16~1 : 64 at 6 weeks old, 1 : 16~1 : 64 at 7 weeks old, and 1 : 16~l : 32 at 8 weeks old. After vaccination with DHPPL to canine parvovirus in 60 days and 80 days old puppies, 1 : 8~l : 32 at 9 weeks old, 1 :16~1 : 128 at 10 weeks old, 1 : 32~1 : 256 at 11 weeks old, 1 : 16~1 : 256 at 12 weeks old, 1 : 128~1 : 256 at 13 weeks old, 1 : 64~l : 512 at 14 weeks old, and 1 : 128~1 : 512 at 15 weeks old. The HI titers of 3 puppies delivered from a mother dog(B) with HI titer of 1 : 512 were 1 : 16 at 1 day old before suckling, 1 : 256~1 : 512 at 2 days old after suckling, 1 : 512 at 1 week old, 1 : 128~1 : 256 at 2 weeks old, 1 : 64~1 : 128 at 3 weeks old, 1 : 64~1 : 128 at 4 weeks old, 1 : 128 at 5 weeks old, 1 : 64~1 : 128 at 6 weeks old, 1 : 16 at 7 weeks old, and 1 : 8 at 8 weeks old. After vaccination with DHPPL to canine parvovirus in 60 day and 80 days old puppies, < : 8~l : 8 at 9 weeks old, < : 8 ~1 : 16 at 10 weeksold, 1 : 64~1 : 128 at 11 weeks old, and 1 : 256~1 : 512 at 12 weeks old. The HI titers of 7 puppies delivered from mother dog(C) with Hl titer 1 : 1,024 were 1 : 512~1 : 1,024 at 2 days old after suckling, 1 : 256~1 : 1,024 at 1 week old, 1 : 256~l : 1,024 at 2 weeks old, 1 : 64~1 : 512 at 3 weeks old, 1 : 64~1 : 512 at 4weeks old, 1 : 8~l : 64 at 5 weeks old, 1 : 8~1 : 64 at 6weeks old, 1 : 8~1 : 32 at 7 weeks old, and < : 8~1 : 8 at 8 weeks old. Antibody to CPV was transferred mainly from mother to progeny through the colostrum and the transferred maternal antibody was in proportion to the HI titer of the mother As the HI titer of maternal antibody in puppies was low, puppies have a rapid immune response and a massive rise in HI titer to vaccination against CPV compared with puppies haying high level of maternal antibody.

• Journal of Food Hygiene and Safety
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• v.33 no.1
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• pp.65-70
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• 2018

Monitoring of Listeria monocytogenes, the causative agent of listeriosis, in food is inportant for public health. The Korean Food Standards Codex has adopted a 'zero-tolerance' policy for L. monocytogenes. The standard detection method of L. monocytogenes is based on enrichment. Thus, proper enrichment methods need to be instituted to ensure quality control of the detection procedures. In this study, the growth of L. monocytogenes and Listeria innocua as a mixed culture in Listeria enrichment broth (LEB) was monitored during artificial contamination of enrichment culture. We confirmed competitive growth or interspecies inhibitory activity of L. monocytogenes and L. innocua. Interspecies growth differences and the inhibitory activity of different inoculation and mixtures L. innocua against L. monocytogenes were examined. The concentration of L. monocytogenes must be 2.0 log CFU/mL or more than L. innocua to grow better than L. innocua. It is known that Listeria spp. and L. monocytogenes show growth difference during LEB, resulting in the risk of false-negative results. The inhibition of L. monocytogenes by L. innocua was always observed when present at lower concentrations. However, it was confirmed that L. innocua suppressed when L. monocytogenes was present at a higher concentration. Therefore if a mixture of Listeria spp. is present, detecting L. monocytogenes is difficult. Thus, a new enrichment broth to improve the detection rate of L. monocytogenes is needed.

• Korean Journal of Ecology and Environment
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• v.41 no.3
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• pp.367-373
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• 2008

A freshwater bivalve (Unio douglasiae) was examined to assess the filtering rate (FR) on the cyanobacterial assemblage in a hypertrophic lake. Animal U. douglasiae used in the present study was collected using a hand-operated dredge from the North Han River (Gapyeong, Korea). The FR was measured at different feeding conditions such as feeding interval (1, 4, 7, and 24 h), mussel size (4.2$\sim$8.1 cm, n=23), prey concentration (506.7, 409.8, 327.5, 199.7 and 88.6 ${\mu}g\;L^{-1}$), and mussel density (0.5, 1.0 and 1.5 indiv. $L^{-1}$). On the applied feeding interval, the maximum FR (0.21 L $g^{-1}h^{-1}$) and minimum feces production (FP, 0.12 mg $g^{-1}h^{-1}$) were observed at 1 and 24 hr, respectively. Both weight-based FR and FP were not correlated with the mussel size, and the values lied in a limited range with some degree of variation. Likewise, no significant relations between FR and FP were observed in the mussel size. The FR values were negatively correlated with food concentration, but positively with FP. For the food concentrations, the maximum FR (0.41 L $g^{-1}h^{-1}$) and FP (0.16 mg $g^{-1}h^{-1}$) were 88.6 ${\mu}gL^{-1}$ and 327.5 ${\mu}gL^{-1}$, respectively. These results indicate that U. douglasiae collected from the North Han River, although the filtering rate were slightly less than Keum River mussel, may be applied as a strategic bio-filter to mitigate cyanobacterial bloom in eutrophic lake.

• Journal of the Korean Chemical Society
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• v.35 no.5
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• pp.527-533
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• 1991

Template condensation reactions of formaldehyde with appropriate tetramines and diamines in the presence of N(II) or Cu(II) ion produce square planar complexes of hexaaza macrotricyclic and macrotetracyclic ligands $[M(L)]^{2+}$(M = Ni(II) or Cu(II); L = 4-methyl-1,3,6,8,11,14-hexaazatricyclo[12,2,1,$1^{8,11}$]octadecane(C), 1,3,10,12,15,18-hexaazatetracyclo[16,2,1,$1^{12,15},0^{4,9}]$docosane(D) 4-methyl-1,3,6,8,12,15-hexaazatricyclo[13,3,1,$1^{8,12}$]eicosane(F), or 1,3,10,12,16,19-hexaazatetracyclo[l7,3,1,$1^{12,16},0^{4,9}]$tetracosane(G). These complexes contain two 1,3-diazacyclopentane or 1,3-diazacyclohexane rings in the six-membered chelate rings. The complexes of C and F contain one methyl group and those of D and G one cyclohexane ring in the five-membered chelate ring. Synthesis and characterization of the Ni(II) and Cu(II) complexes are presented. The effects of the ligand structure on the equilibrium $[Ni(L)]^{2+}2H_2O{\rightleftharpoons}[Ni(L)(H_2O)_2]^{2+}$ in aqueous solutions are described.

• Korean Journal of Food Science and Technology
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• v.19 no.3
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• pp.263-272
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• 1987

Lactobacillus bulgaricus (KFCC 35463) and Kluyveromyres fragilis (KFCC 35458) were inoculated together in soymilk, and then growth characteristics, acid production and the conditions suitable for acid production were investigated. L. bulgaricus produced more acid and the rate of acid production was more rapid when this organism was incubated with K. fragilis in soymilk than when it was incubated singly. Studying the conditions suitable for acid production in soymilk, optimum acid production by the mixed cultures of L. bulgaricus and K. fragilis was achieved with a temperature of $35{\sim}37^{\circ}C$, a 1:2 (O.D.660) ratio of L. bulgaricus to K. fragilis at inoculum, a 1.0% level of sucrose fortification or a 1.5% level of skim milk powder fortification and a culture time of 24hr. Under these conditions the amount of acid produced by the single culture of L. bulgaricus and the mixed cultures of L. bulgaricus and K. fragilis were 0.14% and 0.41%, respectively, in soymilk, 0.13% and 0.70%, respectively, in soymilk fortified with 1.0% level of sucrose. These indicate that the amount of acid produced by mixed cultures is about 2.9-fold greater in soymilk and about 5.4-fold greater in soymilk fortified with 1.0% level of sucrose than that produced by the single culture of L. bulgaricus. The amount of acid produced in soymilk fortified with 1.5% level of skim milk powder was 0.84% level for both of the single culture of L. bulgaricus and the mixed cultures of L. bulgaricus and K. fragilis after 24hr incubation. However, the amount of acid produced by the mixed culture with K. fragilis was greater than that produced by the single culture of L. bulgaricus onlv in soymilk fortified with lower levels of skim milk powder than 1.5%.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.121-127
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• 2009

This study was conducted to improve commercial quality of a cut spray chrysanthemum 'Gama' bred in Korea by foliar application of daminozide (DMZ), suppressing excessive elongation of peduncle caused by high temperature in summer season production. Applications were made at three floral bud developmental stages (I, II, and III), and concentrations used were 0, 500, 1000, and $2000mg{\cdot}L^{-1}$. Both cut flower length and peduncle length showed the greatest suppression of elongation by DMZ treatments at stage II. DMZ also gradually reduced values of those in a concentration-dependant manner. Stem thickness and flower diameter reduced compared to control as DMZ was sprayed at later stage and those two characteristics decreased in a concentration-dependant manner. The number of paralleled flowers neighboring apical part of whole plant showed the best results at stage I and II. As for DMZ concentration, those increased effectively at $500-1,000mg{\cdot}L^{-1}$. Among all combinations, $1,000mg{\cdot}L^{-1}$ DMZ at stage II recorded the most paralleled flowers and showed 24.2% increment as compared to control. The number of fully expanded flowers and total floral buds also showed the highest values using $1,000mg{\cdot}L^{-1}$ DMZ at stage II than the other treatments. In cut spray chrysanthemum cultivar 'Gama' bred in Korea, foliar-spraying with $1,000mg{\cdot}L^{-1}$ DMZ at stage II most effectively suppressed excessive peduncle elongation, parallelled flower cluster arrangement, and increased the number of flowers in summer. However, this combination affected negatively the other commercial qualities, reducing cut flower length, stem thickness, and flower diameter. Therefore, we recommended that foliar-spraying stage and DMZ concentration was stage I and $1,000mg{\cdot}L^{-1}$, respectively, in a practical culture for 'Gama'.

• Korean Journal of Environmental Agriculture
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• v.19 no.1
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• pp.13-19
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• 2000

Seasonal variations of dominant algae species and the effects of these algae on coagulation and filteration of water treatment were investigated at Chilseo water treatment plant in downstream of Nakdong river from January in 1995 to Desember of 1998. The water quality of Nakdong river was found to be a hyper eutrophic state during the investigation periods. In the measurement, Chlorophyll-a contents ranged $20.7{\sim}180.9{\mu}g/l$ and total nitrogen contents(T-N) and total phosphorus contents(T-P) exceeded more than 3.4mg/l and 0.1mg/l, respectively. The changes in dominant algae species was in the order of Stepanodiscus sp., Asterionella sp., Melosira sp., Microcystis sp. and Synedra sp. from spring to winter. Microcystis sp. especially, was blooming during summer and Synedra sp. and Stepanodiscus sp. during winter. Although most diatomous algae appeared in the water treatment process caused filter clogging and reduced efficiency of coagulation and sedimentation, Synedra sp. and Stepanodiscus sp were revealed as the main trouble algae. Malfunction of water treatment process caused by Synedra sp. and Stepanodiscus sp. started at the algae concentrations of 800cells/ml and 1,820cells/ml, respectively. When chlorophyll-a content was $18.9{\mu}g/l$, the optimum amounts of coagulant were found to be 40mg/l of Alum and 16mg/l of PACS. Under condition of chlorophyll-a content of $154.1{\mu}g/l$, addition of Alum at the level of 75mg/l and PACS at the level of 35mg/l showed the lowest turibidity. The result indicates that increased amounts of the coagulants should be added for a better water treatment as chlorophyll-a contents increased. Addition of Alum at the amount of 60mg/l and 30mg/l of PACS removed Stepanodiscus sp. algae at the rate of 85% and 83%, respectively. In case of Synedra sp., 50mg/l of Alum and 25mg/l of PACS showed removal rates of 79% and 81%, respectively. Synedra sp. algae at the standing crops of 1,500cells/ml started filter clogging and a filtering process was completely inhibited after 8 hours. At this situation the filter clogging by Synedra sp. algae occurred at the depth of 5cm from the top anthracite layer. On the other, other algae did filter clogging at the depth of 10cm.

• Journal of the Korean GEO-environmental Society
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• v.17 no.11
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• pp.5-14
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• 2016

The main objective of this study was to evaluate the removal properties of Cu from existing exfoliated vermiculite (EV) coated with a mixed solution of sulfuric acid and glycerol on the $580^{\circ}C$ in heating, which uses coated with glycerol of copper ions can be removed more effectively. Serial batch kinetic tests and batch sorption tests were conducted to determine the removal characteristics for Cu in aqueous solution. The result of batch kinetic test shows that removal rate, $K_{obs}$ (1/hr), of Cu are 0.579, 0.878, 3.459, and 6.578 for MEV weight 1 g (25 g/L), 2 g (50 g/L), 3 g (75 g/L), 4 g (100 g/L), respectively. In this case the initial pH of the solution was 3.26. The removal experiment according to the concentration, $K_{obs}$ (1/hr), of Cu are 1.96, 0.878, 1.25, and 1.04 for the initial concentration of 3 mg/L, 5 mg/L, 8 mg/L, 10 mg/L, and the initial pH of the solution are 3.46, 3.26, 3.10, 2.96, respectively. Influence of initial pHs on Cu removal were tested under 1g of MEV with 5 mg/L of Cu solution. $K_{obs}$ (1/hr) were increased from 0.263 (pH 3) to 0.525 (pH 5). It leads to the conclusion that the removal rates are inversely proportional to the initial Cu concentration and are increased proportional to the initial pHs. Sorption capacity of MEV was determined by batch sorption tests. The maximum sorption capacity ($Q_{max}$) obtained from Langmuir was 0.761 mg/g, Linear and Freundlich partition coefficients were 0.494 L/g and 0.729 L/g (1/n = 0.476). These results show that the MEV could be used as an excellent adsorbent for copper contained in various types of aqueous solutions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.451-456
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• 2004

Based on the data from our previous studies, four new diabetic remedies were composed with the addition of Coicis Semen into Okchun-san (OCH), Commelinae Herba into Gangsim-tang (GST) , Scrophulariae Radix into Sunki-san (SKS), and Erythrinae Cortex into Yukmijihuang-hwan (YMG). The water extracts of these new remedies were treated in 3T3-L1 fibroblasts and adipocytes in order to investigate insulin-like substances and insulin sensitizers, respectively. With and without differentiation inducers, unmodified SKS (SKS-O) treatment induced 3T3-L1 fibroblasts into adipocytes more than the control. However, without inducers, YMG treatment, but not SKS, induced the differentiation more than the control among modified remedies. Without inducers, SKS, OCH as well as YMG increased the induction of differentiation from 3T3-L1 fibroblasts into adipocytes, compared to the control. The treatment of OCH and YMG with 1 ng/mL insulin increased glucose uptake much more than only insulin 1 ng/mL treatment. Thus, OCH and YMG contained increased insulin actions. In conclusions, the modified remedies, OCH and YMG, contained insulin-like substances and insulin sensitizers, and they can be improved the hypoglycemic effects.