• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.154-160
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• 2005

To establish rapid micropropagation through organogenesis from apices-derived callus or direct adventitious shoot of three calla lily cultivars(Zantedeschia spp, cv. Sunlight, cv. Chiante, cv. Pink Persuation) were cultured on Murashige and Skoog medium supplemented with different plant growth regulators. The formation rate of callus, organogenesis and in viかo tuber production among the three cultivars were tested. Callus was obtained from cvs. Sunlight, Chiante and Pink Persuasion; the best cultivar was Sunlight. Sunlight induced $53.3\%$ callus and Chiante had the highest rate of $56.7\%$ direct shoot regeneration on medium with 2.0 mg/L BA. Regeneration frequencies ranged from 20 to $70\%$ on medium with 2.0-3.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoots were obtained on medium containing 3.0 mg/L BA in three cultivars. Cytokinins induced multiple shoot formation; 1.0 mg/L of 2ip, 5.0 mg/L of BA, and 1.0 m/L of BA induced 16, 14 and 12 multiple shoots in cvs. Sunlight, Chiante and Pink Persuasion, respectivly. 1.0 mg/L of IAA enhanced root growth in cvs. Sunlight and Chiante while cv. Pink Persuasion exhibited enhanced root growth at 2.0 mg/L of IBA. NAA, however, induced no change in root growth. The addition of 90 g/L sucrose enhanced in vitro tuber formation and following tuber expansion in cv. Sunlight, while 70 g/L of sucrose was effective in cvs. Chiante and Pink Persuasion.

• Korean Journal of Poultry Science
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• v.27 no.1
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• pp.37-41
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• 2000

This study was carried out to examine the effects of Lactobacillus spp. on performance and cecal microflora in broiler chicks. Eight diets consisting of basal diet(C), C+Lactobacillus crispatus BC7 (T2), C+Lactobacillus reuteri BC9 (T3), C+L.reuteri BC5+L.crispatus BC9 (T4), C+L. reuteri BC5 (T1)+L.reuteri BC9 (T5), C+L.crispatus BC7+L.reuteri BC9 (T6) and C+L.reuteri BC5+L.crispatus BC7+L.reuteri BC9 (T7), were fed to Ross male broiler chicks for 5 weeks. The level of supplemented Lactobacillus spp. was 107 cfu/g diet. Body weight and feed intake were measured every week, and cecal microfla was counted at 1 and 5 weeks for Lactobacillus and yeast. Body weight increased signigicantly in supplemental Lactobacillus treatments from 2 weeks of age (p<0.05). Chicks in T2, T5 and T5 of treatments were heavier than those of other treatments at 5 weeks of age(p<0.05). Viability was not different significantly. Feed intake and feed conversion also were not different, although feed conversion improved slightly in supplemental Lactobacillus treatments. Lactobacillus spp. of cecal content was increased in supplemental Lactobacillus treatments at 1 week, but no significance was found. The number of yeast in cecum was not different from that of supplemental Lactobacillus. In conclusion, Lactobacillus, L.crispatus BC7 and L.reuteri BC9 from broiler cecum could contribute to the increase in body weight with supplemented mono-or mixing of di-lactobacilli. The results indicate that Lactobacillus feeding can benefit to intestinal lactobacillus at early growing broiler.

• Korean Journal of Poultry Science
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• v.4 no.1
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• pp.66-74
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• 1977

닭의 혈액상에 관한 발표는 많으나 아직 우리나라에서의 보고는 거의 없다. 이에 산난능력이 우수한 닭과 병계에 대한 앞으로의 혈액학적 연구를 위한 기초적자료를 얻고저 임상적으로 건강한 소위 한국 재내종(LCH)과 백색레그혼 (WLH)암탉의 정상 혈액학치 즉 적혈구, 백혈구, 혈색소, 헤마토크리프. 평균적혈구 용적, 평균 적혈구 혈색소량. 평균 적혈구 혈색소 농도 등을 조사하였다. 1 R.B.C.는 L.C.H.에서 3.18$\pm$0.10million per c$\mu$. mm였으며 W.L.H.는 3.23$\pm$0.05였다. 2. W.B.C.는 L.C.H에서 42.17$\pm$3.35, thousand per. c$\mu$mm였으며 W.L.H.는 35.64$\pm$2.89였다. 3. Hemoglobin치는 L.C.H.에서 11.24$\pm$0.30gm per 100$m\ell$였으며 WL.H.는 38.40$\pm$0.75였다. 4. Hematocrit치는 L.C.H.에서 34.00$\pm$0,98 percent였으며, W.L.H.는 33.40$\pm$0.75였다. 5. M.C.V.는 L.C.H에서 103.66$\pm$1.22 c$\mu$ 였으며, W.L.H.는 103.32$\pm$1.28이였다. 6. M.C.H,는 C.L.H.에서 34.19$\pm$0.41 $\mu$ $\mu\textrm{g}$9였으며. W.L.H,는 34.37$\pm$0.31이었다. 7. M.C.H.C는 L.C.H.에서 33.09$\pm$0.22 percent였으며, W.L.H.는 33.25$\pm$0.36 0.36이었다. (중략)

• Preventive Nutrition and Food Science
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• v.16 no.2
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• pp.110-116
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• 2011

This study examined the growth effects of yeast hydrolysate (YH) and a traditional Korean herbal mixture (HM, a mixture of safflower seed and gasiogapi extract). Three-week old male SD rats were divided into the following five groups: negative control (saline), positive control (foremilk 0.5 g/kg/day), YH (YH 0.5 g/kg/day), HM (HM 0.2 g/kg/day), and YH+HM (YH 0.5 g/kg/day and HM 0.2 g/kg/day). Tibia bone length was 9.22 mm in the normal control rats, while both the YH and YH+HM groups had significantly longer tibia bones than the control rats (9.75 mm and 10.46 mm, respectively). The proximal epiphyses of YH, HM, and YH+HM measured 0.75, 0.70, and 0.75 mm, respectively, while the length in the control group was 0.50 mm. Plasma insulin growth factor-1 (IGF-1) level was slightly higher in the YH group (1.36 mg/mL) than in the control rats (1.29 mg/mL), but the difference was not significant. Plasma IGF-1 level was significantly increased in the HM (1.49 mg/mL) and YH+HM (1.53 mg/mL) groups compared to the control group (1.29 mg/mL). Growth hormone (GH) levels in YH (17.45 ng/mL), HM (15.49 ng/mL), and YH+HM (16.07 ng/mL) were significantly different compared to the control group (3.63 ng/mL).

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.149-154
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• 2000

Embryogenic suspension cultures were initiated using embryogenic callus from immature inflorescence explants (Aralia cordata Thunb.) cultured on solid MS medium containing 1 mg/L 2,4-D for 8 weeks and then the embryogenic callus was proliferated in liquid MS medium containing 1 mg/L 2,4-D. After sieving the suspensions (pore size 270$\mu$m), embryogenic cells were cultured in liquid MS medium with cytokinins (kinetin, BA, zeatin) for two weeks. When the embryogenic cells were transferred to liquid MS basal medium, primary somatic embryos were developed after 5 weeks of culture. Secondary embryos were developed directly from the primary torpedo and cotyledonary embryos cultured in solid MS basal medium. Frequency of secondary embryogenesis was higher on medium containing 2 mg/L kinetin than the other cytokinins. Plant regeneration was highly recorded by placing secondary cotyledonary embryos induced from primary cotyledonary embryos in MS medium containing 2 mg/L kinetin or 2 mg/L zeatin (25.4% and 28.6%, respectively). The plant regeneration from secordary embryos was prohibited by tertiary embryogenesis.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.245-250
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• 1999

Plantlets derived from leaf blade-segment culture of a variegated tobacco (Nicotiana tabacum L. cv. BY-4) that was induced by a heavy-ion ($^{14}N$) beam irradiation to proembryos, were characterized. When explants from both white and green sections of leaves of the variegated plant were cultured on MS medium containing 0.1 mg/L NAA and 1.0 mg/L BAP, the white sections yielded only white shoots, whereas the green sections generated approximately 47.2% green, 37.4% white and 15.4% variegated shoots. In the F1 generation of a green tobacco derived from the leaf blade-segment culture, the segregation ratio of green to white was 1,651:54. Furthermore, reciprocal crosses showed that all of the progenies was green, indicating that the variegation is not maternally inherited. When the signal intensity of photosynthesis genes was determined by DNA gel blot analysis using the variegated leaves derived from green sections of variegated leaves, there were more of the rbcL, psbA, 16S rDNA and 23S rDNA chloroplast genes in the white sections than the chloroplast genes in wild type and green sections of the variegated plants.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1415-1420
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• 1999

This study was carried out to obtain basic information for breeding materials on the improvement of waxy corn (Zea mays L.). The percents of palmitic, stearic, oleic, linoleic and unsaturated fatty acids in 310 $F_1's$ analyzed were $14.6\;{\pm}\;2.5%,\;2.5\;{\pm}\;1.0%,\;24.8\;{\pm}\;4.2%,\;55.6\;{\pm}\;5.4%\;and\;81.8\;{\pm}\;3.1%$, respectively. The mean value of antioxidative ability was $22.8\;{\pm}\;5.9%$. The average contents of tocopherols, phenolic compounds and carotenoids were $36.2\;{\pm}\;11.5\;{\mu}g/mL,\;120.3\;{\pm}\;27.7\;{\mu}g/mL\;and\;0.4\;{\pm}\;0.6\;{\mu}g/mL$, respectively. The level of oleic acid was significantly correlated with the level of linoleic acid. The electron donating ability was significantly correlated with the level of phenolic compounds and tocopherols but not with the content of carotenoids

• The Korean Journal of Nuclear Medicine
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• v.35 no.1
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• pp.69-74
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• 2001

Purpose: $^{99m}Tc$-HMPAO is a radiopharmaceutical for imaging cerebral blood flow. HMPAO (RR, SS)-4.8-diaza-3,6,6,9-tetramethylundecan-2,10- dione bisoxime) has three stereoismers such as, meso-. d-, and l-HMPAO. Techentium complexes of meso-HMPAO and d,l-HMPAO are known to have different in vivo brain uptakes. In this study, enantiomers of HMPAO (d-HMPAO and l-HMPAO) were separated from d,l-HMPAO. These enantiomers were labeled with $^{99m}Tc$ and the biodistribution studies were performed in mice. Materials and Methods: An intermediate imine product was produced from 2,3-butanedione monooxime and 2,2-dimethyl-1,3-propanediamine (54% yield) and was reduced into a mixture of three isomers (35% yield). The meso-isomer was separated from d,l-mixture by repeated fractional crystallization (11 % yield). The d- and l-enantiomers were subsequently separated by co-crystallization with optical isomers of tartaric acid (25% and 5% yield. respectively). Each enantiomeric HMPAO was labeled with $^{99m}Tc$ by reacting with $SnCI_2{\cdot}2H_2O\;and\;^{99m}Tc$-pertechnetate. Biodistribution study was performed 1 hr after tail vein injection to ICR mice. Results: Radiochemical purities of each compound were over 80%. In biodistribution study. the brain uptakes of d,l- d- and l-form were 1.34, 1.12 and 1.67% ID/g, respectively. In case of l-lsomer the brain uptake was higher (1.5 fold) than d-isomer. Conclusion: We successfully purified each enantiomeric HMPAO. In biodistribution study of stereoismers of $^{99m}Tc$-HMPAO in mice, l-HMPAO may show better brain image than d,l-HMPAO which was supplied in a commercial kit.

• Journal of Bio-Environment Control
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• v.27 no.2
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• pp.140-146
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• 2018

This research was conducted to determine the influence of incorporation levels of sulfur into a coir dust+pine bark medium (1:1, v/v) on the changes in the bicarbonate ($HCO_3{^-}$) concentrations and pH of soil solution, crop growth, and nutrient uptake of 'Ssanta' strawberry. In the preparing of the mixed medium, sulfur powder was added with the rate of 0 (control), 0.23, 0.45, 0.90, and $1.80g{\cdot}L^{-1}$ and Hoagland nutrient solution containing $240mg{\cdot}L^{-1}$ $HCO_3{^-}$ was supplied during the crop cultivation. The growth measurements and tissue analysis for the determination of nutrient contents were carried out 140 days after solution application and the soil solution analysis was performed every two weeks. As the level of sulfur was elevated, the overall growth of mother plants became better showing that the growth indexes except chlorophyll contents were the lowest in control treatment but the statistical differences were not found among the three treatments of $0.45g{\cdot}L^{-1}$ or higher sulfur. The higher the levels of sulfur incorporation, the higher the occurrence of runners and the growth of daughter plants. The length of the runners and the number of daughter plants occurred per mother plants were higher in the treatments of 0.90 and $1.80g{\cdot}L^{-1}$ than the three treatments of 0, 0.23, and $0.45g{\cdot}L^{-1}$, but the statistical differences were not observed between the 0.90 and $1.80g{\cdot}L^{-1}$ treatments. The rose of pH and $HCO_3{^-}$ concentrations in soil solution of root media continued all the cropping period, but those decreased slightly in the treatments of $0.90g{\cdot}L^{-1}$ or higher. The soil solution concentrations of $K^+$ and $PO_4{^3-}$ in the treatments of 0.90 and $1.80g{\cdot}L^{-1}$ was lower than those in other treatments and the statistical differences in the $Ca^{2+}$ and $Mg^{2+}$ concentrations were not observed among all treatments. The nutrient contents in tissue analyzed in this experiment were the lowest in the control treatments and those increased as incorporation rates of sulfur were elevated. Above results indicated that when 'Ssanta' strawberry is grown hydroponically and the root medium is coir dust and the pine bark (5:5, v/v) mix, the sulfur incorporation rate as pre-planting fertilizer has to be higher than $0.9g{\cdot}L^{-1}$ root medium to reduce the $HCO_3{^-}$ injury.

• Journal of Environmental Health Sciences
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• v.16 no.2
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• pp.41-45
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• 1990

This study was performed to analyze for the mineral water (Saeng Su) from 12 pure beverage Co. products, which was obtained during March to May in 1988. The results were as follows: 1. The analytical results of contaminant materials in all the samples were suitable for drinking water standard. The mean value of NH$_{3}$-N, NO$_{3}$-N, Cl-and KMnO$_{4}$ consumption were ND, 49mg/l, 1.08mg/l and 8.14mg/l, respectively. 2. Total bacterial counts were 3/cc in average and Coliforms was negative in all samples. 3. The analytical results of Heavy metals were not detected or detected in a small amount, and the contents were different from each company. The CN, Hg, P were not detected in all the samples and the mean value of Cu, Fe, Mn were 0.0087mg/l, 0.0303mg/l, 0.018mg/l, respectively and it's contents were low. 4. The mean value of Pb, Zn, Cr$^{+6}$, As and Cd were 0.016mg/l, 0.015mg/l, 0.0025mg/l. 0.0059mg/l and 0.0011mg/l respectively.