This study was carried out to estimate genetic correlations among broiler body weights for the selection of broiler breeders at early age, and to investigate the difference of growth rates between male and female in mixed-sex population of broiler from 2wks to 8 wks of age. A total of 1109 records were used in this study. the heritability estimates from combined variance components for 2-,4-, 6- and 8- weeks body weights were 0.32, 0.31, 0.35 and 0.29 respectively. Genetic correlations were 0.91 between 4wks and 8wks body weights, 0.94 between 6wks and 8wks body weights. Records obtained on 4wks and 8wks might be useful in a selection program to improve traits in 8wks broiler weight. Growth rates of male and female from nonlinear equations from 2wks to 8wks were shown differently in both linear and quadratic terms, Even though both sexes showed closely equal body weights at 2wks of age, they manifested great difference about 300g at 8wks of age. At all. ages roles were havier, grew at a faster than female. From this result it might be profitable either to select the combination of lines which has little sex effect or to rear male and female broiler separately up to the marketing body weights.
In order to investigate the effects of water temperature on chemical properties of soil and nutrient uptake of rice plant, four varieties of rice were cultivated in a field where water temperature range was $17.3-23.0^{\circ}C$ and plant samples were taken at three growing stages and were analyzed in detail. The results were summarized as follows: 1. Organic matter content and pH of surface soil were showed no differences while the contents of $P_2O_5$ and $SiO_2$ were increased and potassium was decreased as the water temperature increased. 2. The amounts of nutrient uptake were related to the water temperature and the total nutrient contents of rice plants at harvesting stage were in order of $SiO_2$ > $K_2O$ > T-N > $P_2O_5$ > MgO. The rate of $SiO_2$/N and $K_2O$/N of rice plants grown at water temperature $23.0^{\circ}C$ were over two times to those of the water temperature $17.3^{\circ}C$. 3. Nutrient contents of rice plants showed a increasing tendency as increasing the water temperature. However, nutrient contents such as T-N at whole period, $P_2O_5$ at heading and harvesting stage, and $K_2O$ at heading stage were higher at low water temperature than those of high water temperature. 4. Most of nitrogen was translocated and accumulated in grain, potassium in straw respectively. All nutrients translocated to grain more easily as the water temperature increased.
Red rice (local name "Sore") as a weed has been a serious threat to rice production in direct-seeded rice culture in Ganghwagun, Gyeonggi province. In the Ganghwagun 508 ha out of 1,420 ha in the Samsanmyeon area is infested with red rice. The average lowland rice yield is about 4,300 kg/ha in the Ganghwagun, but the average upland rice yield is about 2,000 kg/ha in infested area. This study was carried out in order to clarify the ecological characteristics of red rice and factors affecting the competitive ability of five red rice varieties, collected from Samsanmyeon in 1981, with rice cultivar. Five varieties-Monggeunsare, Salsare, Ginkaragsare, Galsaegsalsare, Galsaegkaragsare-showed the same morphological characteristics of cultivated Japonica type, Chuncheongbyeo, but red rice tillers more profusely, is taller and lodges more easily than Chucheongbyeo. It shatters easily about 10-15 days after heading date, and at this time the hull is discolored in yellow or dark brown. There are many types of red rice with short or long owns on the spikelet, occasionally with or without own on the spikelet in the same hill, and the grains are short or long. In red reice leaf blast occurs more severely than in cultivated Indica/Japonica type, Teabaegbyeo, particulary serious in Monggeunsare. When red rice invaded in direct-seeded rice, number of panicles of rice became reduced more than other yield components.
The search for tumor-avid agents for use in nuclear medicine imaging or therapy is a field of ongoing importance. Metallothionein (MT) is an intracellular protein that binds many metals with isotopes having imaging or radiotherapeutic potential. The purpose of the study was to determine whether uptake of radioisotopes that bind to MT is increased in tumor. We measured the uptake of Cd-109 and Ga-67 in tumor and normal tissues of sarcoma-bearing mice. Tumors were grown subcutaneously in female Balb/C mice from cultured Balb/3T3 cells transformed by the Moloney murine sarcoma virus (MMSV). When the tumors reached about 1 cm in diameter, mice were injected subcutaneously with Cd-109 and Ga-67. Eighteen and seventy-two hours later, the mice were sacrified. Organs and tissues were removed, weighed, and activity per mg tissue determined by gamma well-counting. Uptake of Cd-109 by MMSV tumors exceeded that by normal tissues examined, with the exception of liver and kidney (the organs known to be richest in MT). The tumor-to-tissue ratios of uptake for Cd-109 were far greater than those for Ga-67 for many normal tissues of great importance in terms of background activity (bone, intestine, fat, muscle, and blood). We concluded that metals that bind to MT may be useful for oncologic imaging or rediotherapy of cancer.
The crop rotation is very important in the organic agriculture and the soybean crop should be included in the rotation. Even though the inoculation of the root can be performed by the soil inoculation in the soybean crop, the effect of the soil inoculation should be proved in the organic soil. For the research of the effects in the formation of the nodule of soybeans, the growth and the quantity through the inoculation into the soil, the field experiment was conducted in 2006 at Ipjang Experimental Farm of Dankook University. The number of nodule in the testing varieties of the inoculated soil plot was $1.4{\sim}2$ times larger than those in the non-inoculated soil plot. The number of nodule was decreased in the non-inoculated soil plot after R2 stage, but it was increased in the inoculated before R4 stage and it was decreased after then. The periodical change of weight of the nodule was shown as same as the number of nodule. The dry weight of inoculated was increased than non-inoculated in Daewonkong, but it was decreased than non-inoculated in Shinpaldalkong2 and SS2-2. The amount of chlorophyll of the inoculated soil plot was more than the non-inoculated soil plot by 9% in Daewonkong, but the inoculated soil plot was less than the non-inoculated soil plot by 13%, 33% each in Shinpaldalkong2 and SS2-2. The number of pods, the number of grains and the weight of grains in the yield characteristics of the inoculated soil plot were more than in the non-inoculated soil plot in Daewonkong, but the number of pods, grains and the weight of grains in the yield result in the inoculated soil plot was less than in the non-inoculated soil plot in Shinpaldalkong2 and SS2-2. The mean yield(per 10a) of Daewonkong was increased 11% in the inoculated soil plot, but the mean yeil(per 10a) of Shinpaldalkong2 was decreased 4% and SS2-2 was also decreased 23%.
The studies were carried out to obtain the basic data for maximizing the protoplast yields from the mycelia of P. ostreatus and P. sajor-caju. Some factors affecting the regeneration of the protoplast of both species and the productivity of their reversion were also examined. The maximum yields of protoplasts were obtained from four days cultured mycelia of both species on cellophan membrane placed on the surface of PSA or MCM media in a petri dish. The optimal concentration of lytic enzyme Novozym 234 for protoplast releasing was 5 mg per ml of 0.5 M phosphate buffer solution with 0.6 M sucrose or 0.6 M $MgSO_4$ at pH 6.0. The greatest number of protoplasts was released 3 hours after incubation of the mycelia of P. ostreatus and after 4 hours for the P. sajor-caju in the lytic enzyme solution. Among the osmotic stabilizer solutions tested 0.6 M sucrose and 0.6 M KCl showed the best regeneration rates of the protoplasts of both species. When 0.75 % agar solution was over-layed on the regeneration media immediately after inoculation of the protoplast the regeneration rates were greatly enhanced. The ampicillin added to the agar solution prevented bacteria from infection. The reverted isolates produced the sporophores and basidial spores just like their parents without any mutations when they were cultivated in a broad mouth bottle with sawdust substrates.
Korean Journal of Agricultural and Forest Meteorology
/
v.4
no.1
/
pp.49-57
/
2002
This study was conducted to find out buffer capacities of forest soils by the treatment of simulated acid rain(SAR) of four forests(Q. spp., P. rigida, P. koraiensis, L. leptolepis) in Kyunghee university's practice forest. All soils of each forest stand were treated by simulated acid rain at the level of pH 3.0, 4.0, 5.0 respectively. The result obtained from this study can be summarized as follows: Soil pH was measured by soil depth of each forest stand. The deeper soil depth was, the higher soil pH was. Also it was appeared that base saturation of sample soils was the highest as 17.42% in P. rigida stand and cation exchange capacity(C.E.C) was the highest as 29.87 me/100 g in Q. spp. stand. for responses of soil leachates to acidification treatment with pH 3.0 simulated acid rain(SAR), as simulated acid rain(SAR)-input was increased, pH value of soil leachates appeared high temporarily, but soon pH value of soil leachates had been low gradually. At the rest of pH 4.0, pH 5.0 treatment, pH value of soil leachates was high proportionably. The amounts of TBC of primary stage had a difference as pH level of simulated acid rain and forest stands. But as simulated acid rain(SAR)-input was increased. Amount of TBC was diminished. Also the amounts of TBC of primary stage in acidification treatment with pH 3.0, 4.0 simulated acid rain(SAR) was higher that of acidification treatment with pH 5.0 simulated acid rain(SAR). These trend showed obvious difference at low soil acidity and high TBC. The amounts of activity Al of primary stage appeared high as increasing the input acidity of simulated acid rain(SAR). Also, by soil depth, the amounts of Activity Al was different between A layer(0-15 cm) and AB layer(0-30 cm). There was considerable the correlation between simulated acid rain-input and activity Al change. But this was oppositional trend in soil leachates of pH 4.0, 5.0 treatment and total base cations(TBC).
The purpose of this study was to investigate the effects of embryo sources such as in vivo vs. in vitro produced blastocyst, and culture systems on the membrane permeability and viability of bovine blastocyst following GMP vitrification. To produce in vivo embryos, six cows were superovulated by administration of follicle stimulation hormone (FSH) and prostaglandin $F_{2{\alpha}}$(PG $F_{2{\alpha}}$). in vitro embryos were produced by two different culture systems, oviduct co-culture (OCS) and defined culture system (HECM-6; DCS). Ovaries were picked up at a local slaughterhouse and transported to laboratory in 3$0^{\circ}C$ saline within 2 h. Ovaries were washed with same saline three times and then placed in saline on warm plate adjusted at 3$0^{\circ}C$ during aspiration. The blastocysts produced were assigned for membrane permeability and viability following GMP vitrification. The membrane permeability of blastocysts was checked in 0.5 M sucrose solution on warm plate at 35$^{\circ}C$ for 0, 2, 5 and 7 min, respectively. Then the diameters (width and length) of embryo cytoplasms were measured by a eyepiece meter, and they were converted to their volume by 4/3 $\pi$$r^3$. The blastocysts were cryopreserved by GMP vitrification method, where they were sequentially placed into vitrification solution before being loaded into GMP vessels and immersed into L$N_2$ within 20 to 25 sec. Post-thaw blastocysts were serially washed in 0.25 and 0.15 M sucrose in HM and TCM-199 for 5 min each, and then cultured in TCM 199 supplemented with 10% FCS for 24 or 48 h. The volume change of in vivo blastocyst at 0, 2, 5 and 7 min (100, 37.1, 34.3 and 31.6%) was significantly more shrunk than those of in vitro blastocysts derived from OCS (100, 59.8, 48.9, 47.9%) and DCS (100, 57.2, 47.3 and 46.9%) (P<0.05). The viability of post-thaw blastocyst derived from in vivo (93.6%) was also significantly different from those in OCS and DCS (81.9 and 83.6%; P<0.05). In the present culture system, the morphology of embryos produced in vitro was similar to that of in vivo embryos, but the quality in membrane permeability and post-thaw viability showed a big difference from their sources as in vivo or in vitro derived from OCS and DCS. The results indicated that the quality of in vivo embryos in membrane permeability and post-thaw viability was better than those of in vitro embryos derived from OCS or DCS.
This experiment was conducted to clarify the effects of source and sink related characteristics on grain weight increase and grain nitrogen accumulation in rice. Source was modified by leaf removing treatment with no sink modification in 2002 and sink and source were modified at the same time by different sowing dates, N fertilization rates, row spacings, and thinning treatments at heading in 2007. Grain weight (GW) and grain assimilates produced by photosynthesis during grain-filling ($GW_P$) increased with the increase of source, while the grain assimilates retranslocated from leaf and stem ($GW_T$) decreased. Among the source-related characters, shoot dry weight were most closely related with GW. GW was dependant on $GW_P$ rather than on $GW_T$. Grain nitrogen content (GN) and grain nitrogen absorbed from soil during grain-filling ($GN_S$) increased with the increase of source, while the grain nitrogen retranslocated from leaf and stem ($GN_T$) decreased. Shoot nitrogen content among the source-related characters was related most closely with GN. The contribution of $GN_T$ to GN was relatively large although GN depended more largely on $GN_S$ than $GN_T$. In addition, GN was supplied firstly from $GN_S$ or from $GN_S$ and $GN_T$ at the same time.
Park, Hyung-Soo;Hwang, Kyung-Jun;Park, Nam-Gun;Kim, Young-Jun;Jeong, Ha-Yeon;Jo, Nam-Chul;Ko, Moon-Suck
Journal of The Korean Society of Grassland and Forage Science
/
v.29
no.1
/
pp.43-50
/
2009
Two experiments were conducted to evaluate the effects of nitrogen (N) application level and regrowth period on the fatty acid (FA) concentration and forage production of tall fescue (Festuca arundinacea Schr.) and bermudagrass (Cynodon dactylon (L.) Pers.). N was applied at 0, 50 and 100 kg/ha, and swards were cut after regrowth periods (15d, 30d and 45d). Concentrations of Individual FA were determined by gas chromatography. FA composition of tall fescue contains a high proportion ($87{\sim}88%$) of total FA contents as palmitic (C16:0), linoleic (C18:2) and linolenic acid (C18:3). The FA composition was not affected by N application, but a longer regrowth period significantly (p<0.05) decreased the composition of linolenic acid (C18:3) and increased those of linoleic acid (C18:2). The FA composition of bermudagrass was not affected by N application, but a longer regrowth period significantly (p<0.001) decreased the composition of linoleic (C18:2) and linolenic acid (C18:3). Linoleic and linolenic acfd of tall fescue were higher than those of bermudagrass. Dry matter (DM) yield of bermudagrass was significantly (P<0.05) affected by N application level and regrowth period. DM yield was higher at high N application and was higher at longer regrowth period. The crude protein concentration was higher at high N application and was low at longer regrowth period. Application of nitrogen fertilizer Increases dry matter (DM) yield and crude protein (CP) content of bermudagrass. These studies demonstrate opportunities to affect the FA concentration and composition of FA in forage through management strategies, which could affect milk FA composition.
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