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Plant Regeneration via Multiple Shoot Formation from Sucker Explant of Hybrid Blackberry (Rubus fruticosus L. ${\times}$ R. parvifolius L.) (교잡종 블랙베리(Rubus fruticosus L. ${\times}$ R. parvifolius L.)의 근맹아 절편체로부터 다경유도를 통한 식물체 재분화)

  • Shin, Jeong-Sun;Lee, Jong-Chon;Sim, Ock-Kyeong;Yoon, Tai-Young;Cho, Han-Jik;Kim, Ee-Yup
    • Korean Journal of Plant Resources
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    • v.21 no.2
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    • pp.111-116
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    • 2008
  • This study was carried out to induce plant regeneration via multiple shoot formation from sucker explants of Rubus fruticosus L. ${\times}$ R. parvifolius L. To induce adventitious shoots, the sucker explants were sterilized in 1.2% sodium hypochlorite (NaOCl) solution, and then were cultured on the full and 1/2 MS solid medium supplemented with BA (0.1, 0.5, 1.0, $2.0mg{\cdot}L^{-1}$). After 4 weeks of culture, the highest frequency (83.3%) of shoot formation from sucker explants was obtained from the full MS medium with $1.0mg{\cdot}L^{-1}$ BA. The highest shoot number (3.7) per explant was obtained from the full MS medium with $0.5mg{\cdot}L^{-1}$ BA. After 12 weeks of culture, the number of shoots (15.4) per explant was increased. The highest frequency (95%) of root formation was obtained from the 1/2 MS medium, when the explant with shoot were cultured on the full, 1/2 and 1/4 MS medium. The survival rate of the plantlets after transfer to plastic pots containing sand, soil, and vermiculite (1.1:1, vol.) was 95%. The results indicate that multiple shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. ${\times}$ R. parvifolius L.

Novel Conversion of 2-(4-Dimethylaminoaryl)-1,4-diphenylbutane-l,4-diones into 3-(4- Dimethylaminoaryl)-l-phenylpropenones via Debenzoylation and Oxidation

  • Kim, Sung-Sik;Chang, Ji-Ae;Kim, Ae-Rhan;Cho, Kyung-Won;Park, Sang-Kyu
    • Journal of Photoscience
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    • v.12 no.2
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    • pp.109-111
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    • 2005
  • It was found that 1,4-diketones such as 1,4-diphenyl-l,4-butanediones containing N,N-dimethylaminophenyl (pDPB) and N,N-dimethylaminonaphthyl (nDPB) at C2 are converted into 3-(4-dimethylaminoaryl)-1-phenylpropan-lones (pPPA and nPPA) by treatment with $Ca(OH)_2$ in methanol, which was easily oxidized to enone, i.e., 3-(4-dimethylaminophenyl)-l-phenylpropenones (pPPE and nPPE), when treated with 2,3-dichloro-5,6-dicyano-l,4-benzoquinone (DDQ) in dichloromethane.

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CENTRAL LIMIT THEOREM ON CHEBYSHEV POLYNOMIALS

  • Ahn, Young-Ho
    • The Pure and Applied Mathematics
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    • v.21 no.4
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    • pp.271-279
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    • 2014
  • Let $T_l$ be a transformation on the interval [-1, 1] defined by Chebyshev polynomial of degree $l(l{\geq}2)$, i.e., $T_l(cos{\theta})=cos(l{\theta})$. In this paper, we consider $T_l$ as a measure preserving transformation on [-1, 1] with an invariant measure $\frac{1}{\sqrt[\pi]{1-x^2}}dx$. We show that If f(x) is a nonconstant step function with finite k-discontinuity points with k < l-1, then it satisfies the Central Limit Theorem. We also give an explicit method how to check whether it satisfies the Central Limit Theorem or not in the cases of general step functions with finite discontinuity points.

Growth retardants stimulate guggulsterone production in the presence of fungal elicitor in fed-batch cultures of Commiphora wightii

  • Suthar, Satish;Ramawat, K.G.
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.9-13
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    • 2010
  • Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the stimulatory effects of growth retardants [ALAR (N,N-dimethylaminosuccinamic acid) and CCC (chlormequat chloride)] and fungal elicitor on guggulsterone accumulation in cell cultures of C. wightii are reported. CCC at $1\;mg\;l^{-1}$ enhanced guggulsterone content (${\sim}123\;{\mu}g\;l^{-1})$ when added on the fifth day after inoculation, while ALAR at $2.5\;{\mu}g\;l^{-1}$ increased guggulsterone content (${\sim}116\;{\mu}g\;l^{-1}$) when added on the tenth day. In a two-stage fed-batch process, combined treatment with fungal elicitor and growth retardant caused a significant increase (${\sim}353\;{\mu}g\;l^{-1}$) in guggulsterone content in cell cultures after 17 days of growth. This represents an approximately fivefold increase over the guggulsterone contents in initial cultures of this plant.

New S-PVA Technology for Advanced LCD Performance

  • Um, Yoon-Sung;Park, Seung-Beom;Kim, Hoon;You, Hye-Ran;Lyu, Jae-Jin;Kim, Kyeong-Hyeon;Kim, Sang-Soo
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08a
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    • pp.403-406
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    • 2007
  • A New S-PVA which is named as charge-shared super PVA (CS S-PVA) LCD mode is proposed. CS SPVA controls the voltage ratio of low pixel by sharing system between low pixel and control cap, resulting in improved off-axis image quality and transmittance increase without any side effects. Moreover, the new S-PVA LCD mode is free of image-sticking. Operating schemes and display performance of CS S-PVA are presented.

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Effect of Temperature, Day length and Sucrose on Bulblet Enlargement of Korean Native Lilies In Vitro (자생나리의 기내 소인경 비대에 미치는 온도, 일장 및 자당의 영향)

  • Eum, Sun Jung;Byun, Mi Soon;Goo, Dae Hoe;Kim, Kiu Weon
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.161-167
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    • 2008
  • Eight species of Korean native lilies were used as plant materials and investigated for the effect of temperature, day length, and sucrose on enlargement of bulblet in vitro. enlargement of bulblet was enhanced at $25^{\circ}C$ in most of Korean native lilies tested, meanwhile it was promoted at $20^{\circ}C$ in L. davuricum and L. hansonii. Moreover, enlargement of bublet was promoted under dark in L. concolor var. parthneion, L. distichum, L. amabile, and L. davuricum, to the contrary fresh weight was increased under longer day length in L. hansonii and L. maximowitzii. That of L. concolor var. parthneion and L. cernuum was not affected by light or dark condition. Fresh weight of the bulblets produced in vitro of most Korean native lilies tested was increased with increasing sucrose concentration and best at $120g{\cdot}L^{-1}$ sucrose in medium. However, enlargement of bublet was promoted by $60g{\cdot}L^{-1}$ sucrose in L. tsingtauense and L. cernuum and by $90g{\cdot}L^{-1}$ in L. davuricum. Bulblet enlargement tended to promote by lower rate of scaly leaf occurrence or separating bulblet and it was affected by occurrence of scaly leaf rather than separation of bulblet. However, occurrence of scaly leaf, separation of bulblet, and enlargement of bulblet were more significantly affected by genetic factors, species rather than culture conditions.

L-Arabinose Production from Diluted Sulfuric Acid Hydrolysis of Corn-fiber (Corn-fiber의 희석된 황산 가수분해에 의한 L-arabinose의 생산)

  • Lee, Hyung-Joo;Lee, Won-Kyu;Ryu, Yeon-Woo
    • KSBB Journal
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    • v.22 no.4
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    • pp.201-206
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    • 2007
  • The demand of L-arabinose has been increased recently because of its advantages including clinical effect. L-arabinose can be produced from dilute acid hydrolysis of agricultural wastes. In this study, optimum conditions of L-arabinose production using dilute acid hydrolysis of agricultural wastes and nutshells were determined. Among the tested various agricultural wastes and nutshells, corn fiber was selected as the best raw material for the production of arabinose. The highest arabinose production was achieved an acid hydrolysis of corn fiber for 1 h at 130$^{\circ}C$ with 0.4% sulfuric acid. Above optimal conditions, it was obtained 20.1 g/L glucose, 10.1 g/L xylose, 7.8 g/L arabinose and 1.8 g/L galactose from 90 g/L of corn fiber. For the purification of arabinose, it was carried out to remove all of sugars except arabinose by the Candida tropicalis cultivation of acid hydrolyzate and an organic contaminants such as pigments by the active carbon treatment of fermentation broth. Moreover, experiments were carried out to eliminate an ions by exchange chromatography. Finally, we obtained 3.1 g of partially purified L-arabinose powder with about 40% yield by evaporation and vacuum drying.

Synthesis and Biological Activity Test of the Sex Pheromone of the Summer Fruit Tortrix Moth (애모무늬잎말이나방의 성 페로몬 합성과 생물활성 시험)

  • Suk-Ku Kang;Sang Soon Ahn;Jung Han Kim;Jeong-Oon Lee
    • Journal of the Korean Chemical Society
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    • v.32 no.1
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    • pp.65-70
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    • 1988
  • Synthesis and biological activity test are described for the (Z)-9-tetradecen-1-yl acetate(1) and (Z)-11-tetradecen-1-yl acetate(1) and (Z)-11-tetradecen-1-yl acetate(2), the sex pheromone of the summer fruit tortrix moth, Adoxophyes orana. 8-Bromoctan-l-ol THP ether was prepared from 8-bromoctan-l-ol. The lithium anion of 1-hexyne was alkylated with 8-bromoctan-l-ol THP ether gave 9-tetradecyn-l-ol THP ether. Catalytic hydrogenation over Pd/BaSO4 followed by deprotection afforded (Z)-9-tetradecen-l-ol. Acetylation gave (Z)-9-tetradecen-1-yl acetate(1). l0-Bromodecan-l-ol THP ether was obtained from l0-bromodecan-l-ol. In liquid ammonia with THF and HMPA as cosolvents, sodium acetylide could be alkylated with 10-bromodecan-l-ol THP ether to give 11-dodecyn-l-ol THP ether. 11-Dodecyn-l-ol THP ether was then treated with n-BuLi in THF to give the lithium acetylide, which was alkylated with bromoethane to afford 11-tetradecyn-l-ol THP ether. Catalytic hydrogenation, deprotection, and acetylative gave (Z)-11-tetradecen-1-yl acetate(2). The synthetic pheromone thus obtained was attractive to the males of the summer fruit tortrix in the field.

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Biological activities of some organometalic compounds as artificial nuclease (인공핵산 분해효소로서 몇 가지 유기금속 화합물들의 생물활성)

  • Sung, Nack-Do;Kim, Dae-Whang;Kwon, Byung-Mok;Kim, Tae-Young;Suh, Il-Hwan
    • The Korean Journal of Pesticide Science
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    • v.4 no.1
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    • pp.32-37
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    • 2000
  • A series of transition metal complexes of 3,6-bis(6'-methyl-2'-pyridyl)pyridazine ($L^{1}$) and 3,6-bis(2'-pyridyl)pyridazine ($L^{2}$) as artificial nuclease, $1{\sim}8$ were synthesized. After determining of X-ray crystal structure, hydrolysis rate constants of phosphates, as DNA model compound and biological activities were confirmed. $L^{2}$-Zn(II) complex, 8 was shown the best hydrolysis rate constant. The $L^{2}$-Ni(II) complex, 5 and $L^{2}$-Co(II) complex, 6 showed the highest herbicidal activity against SCP (Scriptus Juncoids) with excellent tolerance to rice, ORY (Oryzae sativa L.). And the $L^{1}$-Co(II) complex, 2, $L^{1}$-Zn(II) complex, 4 and ligand ($L^{1}$ amp; $L^{2}$) displayed above 90% fungicidal activity against MAG (Magnaporthe grisea).

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Biodegradation of Diesel with Pseudomonas sp, KDi19 in Liquid Medium (Pseudomonas sp. KDi19를 이용한 액체배지내에서 경유의 생물학적 분해)

  • Yun, Min-Woo;Jeong, Jeong-Hwa;Chang, Soon-Woong;Kong, Sung-Ho;Lee, Jong-Yeol;Kang, Dong-Hyo;Lee, Sang-Seob
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.12
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    • pp.1285-1291
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    • 2005
  • In this study, we isolated bacteria from petroleum contaminated soil which were near to underground storage tanks(UST). Through the screen test, we selected high efficiency bacterium, KDi19, for biodegradation of diesel. KDi19 was identified as Pseudomonas sp. by 16S rDNA, fatty acid, and morphological physiological characteristics. KDi19 degraded 956.3 mg/L(95.6%) of 1,000 mg/L diesel for 48 hours(incubation condition : temperature; $30^{\circ}C$, cell concentration; 1.0 g/L, pH 7). At low temperature, $20^{\circ}C$, $15^{\circ}C$, $10^{\circ}C$, KDi19 respectively removed 63.9%, 18.5% and 17.0% of 1,000 mg/L diesel for 48 hours(cell concentration 1.0 g/L, pH 7). At low concentration of diesel, 50 mg/L and 100 mg/L, KDi19 degraded 97.9% and 96.2% of diesel for 24 hours(temperature; $30^{\circ}C$, cell concentration: 1.0 g/L, pH 7), respectively.