• Korean Journal of Microbiology
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• v.35 no.3
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• pp.237-241
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• 1999

Phenolic resin wastewater contained 41,000 mglI phenol, 2,800 mg/l fonualdehyde and various chlorinated phenolic compounds. Candida tropicalis PW-51 isolated [rom the natural enVlfooment was able to degrade 1,000 mg/l phenol in the presence of 100 mglI formaldehyde, but it took much time to degrade phenol with the increase of formaldehyde in phenolic resin wastewater. %en the phenolic resin wastewater was diluted to 1/40, the initial concentration of phenolic compounds (phenols) was 882 mglI and degraded to 81 mglI by C tfVpicalis PW-51 in batch culture. In a continuous biological treatment, the phenolic resin wastewater was diluted to 40 (745 mglI), 20 (1,356 mglI), or 10 (2,875 mglI) times. The removal efficiency of phenols in 1/40- and lI20-diluted phenolic resin wastewater was about 92%, but the phenols in 1!1O-diluted wastewater were not degraded. The remained phenols in wastewater were absorbed by a mixture of activated carbon and rice bran (1:1, v:v) in the process of absorption which was connected to the biological treatment. The total removal efficiency of phenols in 1!40~ and l/20-diluted phenolic resin wastewater was 99.9%.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.426-429
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• 2001

The continuous production of L -proline was studied using L-histidine auxotrophic mutant of Corynebacterium acetoacidophilum under various substrate limited conditions. Among the $NH_4\;^+$ $PO_4\;^3$ and L -histidine limited conditions, the highest production of L -proline was observed under the L-histidine limited condition. Under $NH_4\;^+$ and $PO_4\;^3$ limited conditions, no or poor L-proline production was observed, respectively. For the kinetic parameters under L -histidine limitation the specific rate of L -proline production was increased with dilution rate upto $0.1hr^{-1}$ but decreased above $0.1hr^{-1}$. The volumetric rate of L -proline production was showed similar pattern with specific rate. The dried cell weight was gradually increased according to decrease the dilution rate. Specific rate of glucose consumption was proportionally increased with dilution rate. The results of continuous culture (higher production of L-proline at dilution rate $0.1hr^{-1}$) will be used in fed-batch culture for the control of cell growth rate and mass production of L-proline.

• Research in Plant Disease
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• v.20 no.4
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• pp.299-302
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• 2014

The Paprika plant infected with Pepper mild mottle virus (PMMoV) do not produce commercial fruit as causing necrotic spots symptom on the fruit. Ten cultivars of paprika were analyzed to select the resistance cultivars against PMMoV pathotypes, $P_{1.2}$ and $P_{1.2.3}$, using bioassay and genetic markers. $L^1$, $L^3$, and $L^4$ genotypes expressing resistance to the pathotypes existed in those cultivars but $L^2$ genotype did not. $L^4L^4$ in cvs. Easy and Magnifico, $L^4L^3$ in cvs. Scirocco and Orange glory F1, $L^4L^1$ in cv. Special F1, $L^3L^3$ in cvs. Fiesta, Piero and Derby, and $L^3L^1$ in Cupra and Mazzona F1 were identified with SCAR and CAPS markers. The resistant cvs. to the 2 pathotypes were Magnipico, Easy, Scirocco F1, Orange glory and Special F1 and the susceptible cvs. were Fiesta, Piero, Derby, Cupra and Mazzona F1. The susceptible cvs. of the absence of $L^4$ genotype showed systemic infection when inoculated with PMMoV-$P_{1.2.3}$. However, those cvs. despite the presence of $L^3$ genotype showed vein necrosis on the inoculated leaf and hypersensitive necrosis symptom on the upper parts when inoculated with PMMoV-$P_{1.2}$.

• Phonetics and Speech Sciences
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• v.10 no.1
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• pp.9-14
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• 2018

This study compared the relative contributions of intensity, F0, duration and vowel spectra of L2 English lexical stress by Japanese and Korean learners of English. Recordings of Japanese, Korean and native English speakers reading eighteen 2 to 4 syllable words in a carrier sentence were analyzed using multiple regression to investigate the influence of each acoustic correlate in determining whether a vowel was stressed. The relative contribution of each correlate was calculated by converting the coefficients to percentages. The Japanese learner group showed phonological transfer of L1 phonology to L2 lexical prosody and relied mostly on F0 and duration in manifesting L2 English stress. This is consistent with the results of the previous studies. However, advanced Japanese speakers in the group showed less reliance on F0, and more use of intensity, which is another parameter used in native English stress accents. On the other hand, there was little influence of F0 on L2 English stress by the Korean learners, probably due to the transfer of the Korean intonation pattern to L2 English prosody. Hence, this study shows that L1 transfer happens at the prosodic level for Japanese learners of English and at the intonational level for Korean learners.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.305-308
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• 1998

Leaf and petiole explants of kiwifruit were cultured on MT basal medium supplimented with 2,4-D, kinetin, NAA, and BA. Higher organogenic callus formation was observed on the media with NAA + BA than on the media added with 2,4-D + kinetin. Adventitious buds were formed only on media with NAA and BA. Leaf was better explant than petiole. When callus and adventitious buds were subcultured, shoot formation responsed best on medium with 0.1 mg/L NAA + 2.0 mg/L zeatin. When shoots were cultured on medium with 0.5 mg/L IAA + 0.1 mg/L BA after soaking for 1 hr at IBA solution, rooting was more effective than non-IBA treatment. Rooted shoots developed into normal plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.537-541
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• 1994

This study was conducted to investigate the possibility of callus induction and plant regeneration from immature inflorescence, stem and petiole of A. koreana MAX. which is worth enough to be used as food and medicine. The callus induction and its proliferation was best when immature inflorescence segments were placed on MS medium supplemented with 2, 4-D 2mg / l. The white and compact embryogenic callus on the surface of dark yellow and soft callus which was induced from immature inflorescence segments came into being only on MS medium with 2, 4-D 1mg /l and 2mg /l, but didn't come into being on the other ones. The shoot came into being effectively from callus derived from immature inflorescence on MS medium mixed 2, 4-D 0. 1mg /l with Kinetin 1mg /l, and 2, 4-D 0.5mg /1 with Kinetin 2mg /l. Immature inflorescence was most appropriate material for callus induction and plant regeneration.

• Korean Journal of Plant Tissue Culture
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• v.21 no.2
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• pp.117-121
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• 1994

The optimal level of growth regulator for callus initiation stem explants was BAP 0.1mg/L combined with 2,4-D 1.0 mg/L in Murashige and Skoog (MS) medium supplemented with 30g/L sucrose and 10g/L agar, and that for cell growth was BAP 0.1+2,4-D 0.5 mg/L in MS liquid medium. The regeneration frequency of P. oleracea cells was significantly increased by subjecting the cells to dessication for 1and 2 h up to 83%, respectively, as compared with untreated control showing 61%. Cell viability and survival rate was inhibited by pretreatment of 0.6% NaCl for 2 days, while regeneration ability was not affected by the treatment. Pretreatment of 100g/L sucrose for 2 days markedly stimulated the regeneration of cells up to 81%. These results suggest that in addition to physiological changes, water stress resulted from dessication and high concentration of sucrose and NaCl is closely related to the regeneration of P. oleracea cultured cells.

• Journal of the Korean Chemical Society
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• v.32 no.1
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• pp.60-64
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• 1988

Synthesis and biological activity test are described for the (Z)-11-hexadecen-1-ol, (Z)-11-hexadecen-1-yl acetate, and (Z)-11-hexadecen-l-al, the sex pheromone of the diamond back moth, Plutella xylostella L.. Lithium acetylide was alkylated with 10-bromodecan-1-ol THP ether to give 11-hexadecyn-l-ol THP ether. 11-Hexadecyn-l-ol THP ether was stereoselectively reduced over Pd/BaSO4to yield (Z)-11-hexadecen-l-ol THP ether, which was in turn deprotected to provide (Z)-11-hexadecen-l-ol. (Z)-11-Hexadecen-l-ol was acetylated and oxidized to afford (Z)-11-hexadecen-1-yl acetate and (Z)-11-hexadecen-l-al, respectively. Biological activity test of the synthetic compounds, (Z)-11-hexadecen-l-ol, (Z)-11-hexadecen-1-yl acetate, and (Z)-11-hexadecen-l-al in the ratio of 0.1 : 5 : 5 was tested in the field using polyethylene capsules as containers. The numbers of moth trapped with pheromone vials were counted.

• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.23-27
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• 2006

Molecular subtyping of Listeria monocytogenes, including type strains and isolates from Korean foods, were performed using random amplification of polymorphic DNA (RAPD). Each Listeria species showed specific RAPD band patterns, and L. monocytogenes serotypes and isolates were divided into two clusters. RAPD results showed that L. monocytogenes isolates from Korean foods were divided into two groups. Group I contained L. monocytogenes serotypes 1/2b and 4b, whereas Group II contained serotypes 1/2a and 1/2c. These results suggested RAPD as possible subtyping methods for Listeria species. Also, RAPD Results showed significant correlation between molecular subtyping and serotyping of L. monocytogenes, and classified two different groups of L. monocytogenes isolated from Korean foods.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.447-451
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• 2000

This study was conducted to establish a regeneration system of plantlets through callus culture induced from bulb scales of Lillium‘Gelria’. Friable callus was induced very easily from bulb scales, and grew vigorously on medium lacking growth regulators. In media with 0.5∼ 1.0 mg/L kinetin and 0.1 ∼ 1.0 mg/L NAA, 100% of explants produced callus. Proliferation of callus was actively occurred on media containing 0.1 ∼ 1.0 mg/L kinetin and 0.1 ∼ 1.0 mg/L NAA. Callus proliferation and regeneration of bulblets from callus were occurred simultaneously. Light condition was more effective for the callus proliferation and solid medium was better than liquid medium. Althrough callus was proliferated vigorously on media containing 0.1 ∼ 1.0 mg/L BA and NAA, the frequncy of plantlet regeneration was better on medium without growth regulators, then on medium with 0.1 mg/L BA and NAA.