• Korean Journal of Environmental Biology
• /
• v.27 no.4
• /
• pp.414-424
• /
• 2009

The research was to get a biological assessment on Bokha stream and Dal stream, using the physicochemical factors and the epilithic diatom from June, 2008 to March, 2009. As a result, Bokha stream showed high consistency of total phosphorus ($0.041{\sim}0.886\;mg{\cdot}L^{-1}$), and total nitrogen ($0.336{\sim}10.532\;mg{\cdot}L^{-1}$). Also, the concentration of BOD showed most of the sites polluted as $2{\sim}5\;mg\cdot}L^{-1}$ except for the upstream sites. However, excluding downstream sites and other few sites, Dal stream had very clean water quality having total phosphorus less than $0.06\;mg\cdot}L^{-1}$, and total nitrogen less than $2.0\;mg\cdot}L^{-1}$. Out of the 12 total sites, 98 taxa of epilithic diatoms appeared: 67 taxa from Bokha stream, and 78 taxa from Dal stream. The results of the water quality assessment showed that, Bokha stream, having TDI level over 80, was mostly $\alpha$-mesosaprobic, which indicates a severe pollution. On the other hand, Dal stream, having TDI level below 50, was examined to be xenosaprobic and $\alpha$-oligosaprobic, which indicates a clean water quality. The correlation between DAIpo and TDI measured to be high in correlation coefficent (0.83) from the result of correlation analysis. When the water quality is good, there is an inclination of getting high correlation between chemical and biological water quality assessment results. TDI, which uses numerous taxa of diatoms, showed more stability than DAIpo, which uses less amount of taxa of diatoms to get its result.

• Journal of Life Science
• /
• v.26 no.5
• /
• pp.571-583
• /
• 2016

Biogenic amines are produced primarily by microorganisms found in fermented foods and are often implicated in poisoning incidents in humans. In this study, 620 strains of microorganisms were isolated from traditional Korean fermented food in Sunchang in order to screen for non-biogenicamine-producing microorganisms present in these foods. One strain was identified and named Bacillus subtilis SCJ1, by using 16S rRNA sequencing and biochemical characterization. We investigated the cell growth of this organism in order to understand its potential for industrial application. To this end, we optimized the culture medium constituents by using the response surface methodology. The Plackett-Burman experimental design was used for screening of the medium constituents, such as molasses, yeast extract and peptone, for improving cell growth. In order to determine the optimal concentration of each constituent, we used a central composite design. Consequently, the optimized concentrations of molasses, yeast extract and peptone were predicted to be 27.5 g/l, 7.5 g/l and 17.5 g/l, respectively. By model verification, we confirmed that a 1.49-fold increase in dry cell weight compared to the basal medium-from 1.32 g/l, to 1.9722 g/l-was achieved.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.4
• /
• pp.769-777
• /
• 2017

To research the effects of marigold extract, which is used mixed with calendula extract, on collagen growth and MMP-1 expression in human fibroblast, we measured cytotoxicity, collagen growth and MMP-1 expression by using HDF cells. The result of measurement showed over 80% cell survival rate in $5{\sim}100{\mu}g/mL$ concentration of marigold extract and calendula extract for HDF cells, which indicates there is no cytotoxicity. The result of measuring collagen synthetic abilities showed both types of extract had collagen synthetic ability increase dose dependently, by 25% in $100{\mu}g/mL$ concentration of marigold extract, and by 7% in $100{\mu}g/mL$ concentration of calendula extract. The result of experimenting the effect on MMP-1 expression showed that both types of extract suppress MMP-1 expression. The result of observing phosphorylation of p-JNK and p-ERK, which are known to be involved with MMP-1 expression, revealed that marigold extract effectively suppresses MMP-1 expression through signaling pathway of p-JNK and p-ERK. The above results confirm the wrinkle improvement effect of marigold extract, and furthermore, it can be used as a cosmetic ingredient for anti-aging.

• Bulletin of the Korean Chemical Society
• /
• v.31 no.9
• /
• pp.2701-2704
• /
• 2010

This work shows that both L2 and L3 bearing two and four N-$(CH_2)_2OCH_3$ groups, respectively, can be prepared selectively by the reaction of $L^1$ with 1-bromo-2-methoxyethane. The di-N-substituted macrocycle $L^2$ readily forms its copper(II) and nickel(II) complexes. The N-$(CH_2)_2OCH_3$ groups in $[CuL^2]^{2+}$ are coordinated to the metal ion, whereas those in $[NiL^2]^{2+}$ are not involved in coordination. Interestingly, $L^3$ reacts with $Cu^{2+}$ ion to form $[Cu(HL^3)]^{3+}$, in which one tertiary amino group is not involved in coordination.

• Journal of Korean Medicine for Obesity Research
• /
• v.4 no.1
• /
• pp.125-137
• /
• 2004

Objective: The purpose of this study is to investigate how to use the Quality of Life(QoL) of obesity and to study the report cases which measure QoL on obesity. Method: This study investigate the definition of Health-related Quality of Life(HRQoL), the measurement of Health-related Quality of Life, and the papers of Health-related Quality of Life on obesity through the books and medicine journals of HRQoL and obesity. Conclusions: 1. The QoL includes role functional ability, the degree and quality of social and community interaction, psychological state, somatic feeling, and life satisfaction than personal health and social well-being. 2. The investigator needs to evaluate the reliability, validity and sensitivity of the scale, and the appropriateness of the instrument for the target population When he decides measurement. 3. The investigator have the well-drilled supporter or himself managed the data and study population to prevent missing data. 4. The investigator should decide which is needed on obesity a obesity-specific or broad-ranging instrument, or both.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.57 no.4
• /
• pp.16-24
• /
• 2015

Desalination technology is a process to remove salt from water. There are three classified In accordance with the concentration of salt The concentration of sea water 15,000~50,000mg/l, brackish water 1,500~15,000mg/l, desalination less than 500mg/l.. In general, salt to remove for using a pre-treatment UF filter, but this study is new pre-treatment technology RO Membrane process technology Suspended particulate matter is said most were treated at the pre-treatment equipment, wheat affluent particulate material was removed from the MF filter. Influent SS 16.2mg /l The treatment was effective in treatment 0.05mg /l of 99% is removed. COD is reduced to 60% in the pre-treatment device, after treatment was reduced to 30% RO membrane. Influent COD 10.2mg/l treatment was removed 1.9mg/l. The removal rate is 81.9%. Desalination removes the ionic substances in the RO Membrane. Influent EC $978.8{\mu}s/cm$ and treatment showed a result of $18.7{\mu}s/cm$.

• Nutrition Research and Practice
• /
• v.8 no.5
• /
• pp.516-520
• /
• 2014

BACKGROUND/OBJECTIVES: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes. MATERIALS/METHODS: 3T3-L1 adipocytes were treated with CA ($0-20{\mu}M$) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured. RESULTS: LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNF)-${\alpha}$, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-${\kappa}B$, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA. CONCLUSIONS: Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.

• Journal of Environmental Health Sciences
• /
• v.21 no.1
• /
• pp.47-55
• /
• 1995

Minari(Oenanthe stolonifera DC.) lived in serious polluted water is able to remove chemical elements from batch system. This study attempted to compare the incorporation of cadmium and lead in batch system. Different concentrations of Cd(0.02 0.1 0.5 1.00 5.00 mg/l) and Pb(0.1 0.5 1.0 5.0 10.0 mg/l) were used. Toxic effects were obvious at Cd 1.00 mg/l and Pb 5.00 mg/l. The detrimental effects on minari were chlorosis of leaves and stunted roots and reduction in the number of lateral roots. There was reduction in growth rates exposed above Cd 1.00 mg/l and lead 5.0 mg/l. The minari capacities to remove Cd were 34.1-74.2% and Pb were 53.0~91.5%. The removal rates by minari in Cd and Pb solution were decreased with increased exposure concentration(in Cd r=0.97, in Pb r=0.88). The removal rates by minari in Cd and Pb solution were increased with increased growth rate(in Cd r=0.93 in Pb r=0.92). Recovery rates on minari are 67.3~95.2% in Cd water and 72.6~88.3% in Pb water. The rates are increased with increased growth rates and decreased initial concentrations.

• Environmental Analysis Health and Toxicology
• /
• v.18 no.3
• /
• pp.219-224
• /
• 2003

1,4-Dioxane is used as a solvent for lacquers, paints, varnish removers, dye baths and printing compositions. And it is also used for detergent preparations, cosmetics, deodorants and fumigants. A method is described for the determination of 1,4-dioxane in water samples by GC/MS. The extraction recoveries were studied for some solvents and solvent volume ratio were investigated using r-butyl methyl ether (MTBE). Optimum condition was obtained by the liquid-liquid extraction using the 10 mL of MTBE for 10 mL of water. Method detection limit of 1,4-dioxane in the 20 mL of water samples was 0.05 ng/mL. It could be determined in the range of 0.24∼240 ng/mL in treated water, and in the range of 0.69∼81.9 ng/mL in raw water, respectively. Risk assessments with 1,4-dioxane exposure by drinking water ingestion were carried out. Based on the results of analysis, chronic daily intake of 1,4-dioxane was 2.22${\times}$10$\^$-4/ mg/kg/day and excess cancer risk was calcu-lated to be 2.44${\times}$10$\^$-6/.

• The Korea Journal of Herbology
• /
• v.39 no.3
• /
• pp.49-56
• /
• 2024

Objective : Obesity, which has recently been rapidly increasing in the obese population, is caused by an imbalance in energy intake and consumption. The reason why we need to manage obesity well is that the prevalence of complications such as diabetes, atherosclerosis, insulin resistance, and cardiovascular disease increases. In this study, the effect of FO (Fragaria orientalis) water extract on fat metabolism in 3T3-L1 cells was observed to develop a new anti-obesity material based on Mongolian medical books. Methods : The effect of FO extract on adipogenesis in 3T3-L1 cells was observed using DPPH scavenging, pancreatic lipase inhibitory activity, MTT analysis and Oil-red-O staining method. And the expression of proteins related to lipid metabolism was analyzed by Western blot. Results : The FO group significantly increased the DPPH radical scavenging activity at 5 mg/ml compared to the positive control BHA at 0.1 mg/ml. In oil red O staining at a safe concentration without cytotoxicity, lipid accumulation was significantly inhibited by less than 80% compared to the control group at all concentrations. Moreover, treatment of FO significantly increased the expression of proteins related to lipid metabolism, such as p-AMPK and p-ACC, in 3T3-L1 cells, and the expression of CPT-1 tended to increase in a dose-dependent manner. However, the expression of PPAR-γ was significantly decreased in a dose-dependent manner. Conclusion : These results suggest that FO water extract has a potential anti-obesity effect and are expected to be utilized in the development of materials for obesity prevention and treatment.