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The Physiological Activities of Bark Extract of Albizia julibrissin (자귀나무(A. julibrissin) 수피 추출물의 생리활성)

  • Lee, Yang-Suk;Kim, Kwang-Kon;Kim, Nam-Woo
    • Food Science and Preservation
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    • v.18 no.1
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    • pp.79-86
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    • 2011
  • Three bark extracts of Albizia julibrissin were prepared using water (AW), 70% (v/v) ethanol (AE), and hot water (AHW). Organic solvent fractions were analyzed for total flavonoids and polyphenols, antioxidant activities, and inhibitory activities against xanthine oxidase. Total flavonoid and polyphenol contents of the AHW extract were 8.57 mg/g and 108.67 mg/g, respectively. The SOD-like activities of all extracts, assayed at 1.0 mg/mL, were 10.46-16.73%. The nitrite-scavenging ability of the AHW extract, assayed at pH 1.2, was 60.82%, and the $IC_{50}$ value was $770.18\;{\mu}g$/mL. The electron-donating ability of the AHW extract, at 0.3 mg/mL, was 92.30%; the $IC_{50}$ values of the AW and AHW extracts were $31.31\;{\mu}g$/mL and $36.22\;{\mu}g$/mL, respectively; thus higher than that of ascorbic acid ($39.06\;{\mu}g$/mL). Xanthine oxidase inhibition by the AHW extract, at 1.0 mg/mL, was 94.05%. These results indicate that the AHW of A. julibrissin has potential as a natural antioxidant, for addition to foods and nutraceuticals.

Scopolamine Production in Suspension Cultures of Tumor Calli from Datura metel L. (흰독말풀(Datura metel L.)종양 캘러스의 현탁배양으로부터 Scopolamine 생성)

  • 이수경;윤길영;김용해;양덕조
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.203-211
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    • 2000
  • In this study to produce large-scale scopolamine we were examined in the tumor calli of Datura metel L. induced by Agrobacterium tumefaciens $Ery{101}$. The growth and scopolamine contents of tumor calli were higher under light condition than in dark. The optimum condition of growth and scopolamine production were fluence rate of 16 $\mu$mol $m^{-2}s^{-1}$, spectra of red light region and 16 hour light periods on 50 mL SH liquid medium in 4 weeks culture. To increase of the scopolamine contents in tumor calli, the optimum concentration of nitrogen source were 1.8 mM NH$_4$+ and 40 mM NO$_3$. The optimum elicitor concentration for production of scopolamine were 10 mg/L chitosan and 15 mg/L yeast extract. The effect of precursors were good at the concentration of 0.2 mM tropine and 0.3 mM tropic acid, respectively. In order to increase of growth and scopolamine contents. we induced mutant from Datura metel L. tumor callus. Mutants of tumor calli were obtained by 3 Krad, 4 Krad and 6 Krad of ${60}^Cor-ray$. Among them, 3 Krad tumor callus was excellent on the growth and teratoma induction. The 4 Krad tumor callus was negligible for both growth and teratoma induction. But the 6 Krad tumor callus was the best in growth and teratoma induction. The formation of the mutant calli can be enhanced through hormonal combination of 1 mg/L 2,4-dichlorophenoxyacetic acid and 0.5 mg/L benzyladenine. We carry out selection mutant tumor calli for high content tropane alkaloid and suspension cultures for scopolamine production.

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Effect of Compression Test Conditions on Texture Profile Analysis of Surimi-based Products (압착실험조건이 어육연제품의 조직감 면모분석에 미치는 영향)

  • Lee, Young-Seung;Yoo, Byoung-Seung
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.225-230
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    • 2000
  • The texture profile analysis (TPA) parameters of commercial surimi-based products were evaluated at various compression test conditions. Cylindrical specimens of diameter to length (D/L) ratio of 1.0, 1.5, B.() cm/min were compressed to 50, 65 and 80% using crosshead speeds of 1.7, 3.3, 6.7 cm/min. TPA parameters of three surimi-based products (hardness-1, hardness-2, cohesiveness, springiness, chewiness and gumminess) were analyzed statistically. The TPA parameters were modified by dividing with cross section area and strain. The TPA parameters were affected by D/L and compression ratio except for crosshead speed for all three surimi-based products. The ecommended test conditions for TPA of surimi-based products seem to be D/L of 1.5, compression ratio of 80%, and crosshead speed of 1.7 to 6.7 cm/min.

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Effect of Korean red ginseng marc fermented by Bacillus subtilis on swine immunity

  • Kim, Hong-Kook;Choe, Yeong-Ho;Kim, Geun-Seop;Kim, Ha-Young;Kim, Byeong-Soo
    • Korean Journal of Veterinary Service
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    • v.41 no.3
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    • pp.141-147
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    • 2018
  • Red ginseng marc is a by-product of Korean red ginseng (panax ginseng CA Meyer) and contains ginsenoside which has pharmacological effects. The Korean red ginseng marc was fermented with Bacillus subtilis (RGMB). This study was carried out to investigate the RGMB effect on swine immunity. The variation of ginsenoside depending on the RGMB fermentation time was analyzed. Swine (Landrace${\times}$Yorkshire) were divided into control group (basic diet) and RGMB group (RGMB 1% diet). One percent RGMB was fed to the RGMB group for 28 days. The biochemical parameters, cytokine and immunoglobulin were analyzed. For 48 hours of fermentation on RGMB, ginsenoside Rb1 had increased 180.94%, Rg3 235.85%. Rg1 wasn't detected before fermentation, but was detected after 48 hours of fermentation. The RGMB had effect of deceasing initial AST concentration $79.33{\pm}12.85U/L$ to $54.00{\pm}14.46U/L$ in final and was significantly lower (P<0.05) than control in final. In final RGMB had significantly lower (P<0.05) ALT concentration of $48.57{\pm}8.26U/L$ comparing with control group of $65.43{\pm}10.31U/L$. RGMB had the effect of significantly decreasing (P<0.05) $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ concentration of $2.44{\pm}1.31ng/mL$, $0.71{\pm}0.36ng/mL$ and $0.51{\pm}0.21ng/mL$. The IgA concentration had significantly increased (P<0.05) in RGMB group of $0.56{\pm}0.06mg/mL$ in final. These results demonstrate that RGMB has effect of increasing immunity and practicable to use as feed additives on swine.

Study on Wood Structure and Fiber Characteristics of Genus Lespedeza species (싸리나무류재(類材)의 조직(組織)과 섬유(纖維)에 관(關)한 연구(硏究))

  • Kim, Su-In;Yang, Chang-Sul
    • Journal of the Korean Wood Science and Technology
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    • v.16 no.1
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    • pp.9-20
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    • 1988
  • Lespedeza species have been widely used not only as plant resources for bark, leaves and honey, but also as erosion control materials. This study was carried out to investigate the structural and wood tiber characteristics in order to obtain basic information for the utilization of the wood. The wood structure was investigated for 10 selected species and the wood fiber, for the 5 selected species among Lespedeza species distributed all over the country. The following results were obtained. In the cross section, campylotropis showed ray diffuse porous wood, L. bicolor, L. cyrtobotrya, L. thunbergii var. intermedia, diffuse porous wood, and L. tomentella, L. angustifolioides. L. maritima, L. robusta, L. patentibicolor, ring porous wood. The maximum diameter of a single vessel ranged 66-123 ${\mu}$ in all species. Campylotropis showed the most number of vessels, L. angustifolioides, the least. The number of ray per mm ranged 7-22, Campylotropis showed the most number of rays, L. angustifolioides, the least. In the radial section the average length of vessel ranged 121-250 ${\mu}$ in all species. L. thunbergii var. intermedia showed the longest vessel, L. tomentella, the shortest. In tangential section the average width of the uniseriate ray ranged 9.2-14.7${\mu}$, that of the multiseriate ray, 19.2-42.1 ${\mu}$. The average height of the uniseriate ray ranged 143.0-1162.0 ${\mu}$. The width of fiber ranged 10.12-13.61 ${\mu}$, L. maximowiezii showed the narrowest tiber, L. thunbergii var. intermedia. the widest, the thickness of fiber wall ranged 2.93-3.71 ${\mu}$ in the five species. L. maximowiezii showed the most thin fiber wall, L. thunbergii var. intermedia, the thickest, L. cyrtobotrya showed the difference in the size of fiber between the shade and the sunny sites but L. maximowiezii showed no difference. There was significant difference in fiber length, fiber width and wall thickness between L. eyrtobotrya and L. maximowiezii.

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Selection and Treatment Effect of Plant Growth Retardants on Potted Spathiphyllum Grown in a Recirculating Subirrigation System (순환식 저면관수 시스템을 이용한 스파티필럼의 생장조절제 선발과 처리효과)

  • Won, Eun Jeong;Park, Yoo Gyeong;Jeong, Byoung Ryong
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.2
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    • pp.81-88
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    • 2011
  • Effect of concentrations of different plant growth retardants (PGRs) supplied to a recirculated nutrient solution in an ebb and flow system on the growth and development of potted Spathiphyllum 'Top-Pin' and 'Mini' was examined. Plants were planted in 10 cm diameter plastic pots filled with a mixture of peat moss and perlite (1 : 1, v/v) on 30 June 2005 and grown until 23 Sep. 2005. In a closed ebb and flow system, 50, 200, 350, $500mg{\cdot}L^{-1}$ daminozide (B-9), 10, 40, 70, $100mg{\cdot}L^{-1}$ paclobutrazol (Boundy), 5, 15, 25, $35mg{\cdot}L^{-1}$ ethephon (Florel), and 1, 4, 7, $10mg{\cdot}L^{-1}$ uniconazole (Sumagic) were supplemented to a nutrient solution at the initiation of experiment. On every irrigation, the nutrient solution containing PGRs was supplied at a 2 cm depth and kept for 15-20 minutes. The surplus nutrient solution was drained back into the tank for next irrigations. Paclobutrazol gave the most pronounced effect in inhibition of stretchiness. The greatest reduction of leaf length, fresh and dry weights of shoot, and elevated chlorophyll content were recorded in Spathiphyllum, with increasing paclobutrazol concentration. Daminozide concentration greater than $200mg{\cdot}L^{-1}$ resulted in minor stunting. The lowest concentration ($1mg{\cdot}L^{-1}$) of uniconazole showed greater leaf length, leaf width, and leaf petiole length than the control ($0mg{\cdot}L^{-1}$). Uniconazole concentration greater than $1mg{\cdot}L^{-1}$ resulted in similar plant growth as the plant in the control ($0mg{\cdot}L^{-1}$). High concentration ($35mg{\cdot}L^{-1}$) of ethephon resulted in the shortest leaf length, and the greatest chlorophyll content. Inhibition of stretchiness was observed even in the lowest concentration treatments. Among the PGRs, paclobutrazol was most effective in suppressing plant stretchiness. In both cultivars, ethephon and paclobutrazol, but not daminozide and uniconazole, significantly inhibited stretchiness. The results suggested that plant growth retardants (ethephon or paclobutrazol) selected in this study may be used as the most effective agents for inhibition of stretchiness in Spathiphyllum.

HIGHER CYCLOTOMIC UNITS FOR MOTIVIC COHOMOLOGY

  • Myung, Sung
    • Korean Journal of Mathematics
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    • v.21 no.3
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    • pp.331-344
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    • 2013
  • In the present article, we describe specific elements in a motivic cohomology group $H^1_{\mathcal{M}}(Spec\mathbb{Q}({\zeta}_l),\;\mathbb{Z}(2))$ of cyclotomic fields, which generate a subgroup of finite index for an odd prime $l$. As $H^1_{\mathcal{M}}(Spec\mathbb{Q}({\zeta}_l),\;\mathbb{Z}(1))$ is identified with the group of units in the ring of integers in $\mathbb{Q}({\zeta}_l)$ and cyclotomic units generate a subgroup of finite index, these elements play similar roles in the motivic cohomology group.

Kinetics of Strictly Anaerobic Ethanol Fermentation from Starch by Clostridium thermohydrosulfuricum

  • PARK, YOUNG-MIN;CHUL-HO KIM;SANG-KI RHEE
    • Journal of Microbiology and Biotechnology
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    • v.2 no.4
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    • pp.288-292
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    • 1992
  • Kinetics of anaerobic ethanol fermentation by Clostridium thermohydrosulfuricum were investigated for the one-step production of ethanol from starch. A mutant strain with a high ethanol yield was induced from C. thermohydrosulfuricum. The mutant, designated as ME4, produced anaerobically 6.1 g/l of ethanol, 3.1 g/l of lactate and 0.1 g/l of acetate from 20 g/l of starch at $68^{\circ}C.

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Kullback-Leibler Information in View of an Extended Version of κ-Records

  • Ahmadi, Mosayeba;Mohtashami Borzadaran, G.R.
    • Communications for Statistical Applications and Methods
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    • v.20 no.1
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    • pp.1-13
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    • 2013
  • This paper introduces an extended version of ${\kappa}$-records. Kullback-Leibler (K-L) information between two generalized distributions arising from ${\kappa}$-records is derived; subsequently, it is shown that K-L information does not depend on the baseline distribution. The behavior of K-L information for order statistics and ${\kappa}$-records, is studied. The exact expressions for K-L information between distributions of order statistics and upper (lower) ${\kappa}$-records are obtained and some special cases are provided.