• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.124-128
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• 2005

This studies were carried out to investigate optimal conditions for Lactic acid bacteria growth, which was grown in a batch fermenter. The optimal temperature was $30^{\circ}C$, optimal pH was 6.5 and agitation speed was 100rpm and didn't supply the air. Used media compositions were yeast extract 5g/L, peptone 10g/L, sugar 20g/L, beef extract 10g/L, tween 80 1ml/L, ammonium citrate 2g/L, sodium acetate 5g/L, magnesium sulfate 0.1g/L, manganese sulfate 0.05g/L, dipotassium phosphate 2g/L. These results would be useful for enhancing lactic acid bacteria concentration.

• Food Science of Animal Resources
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• v.35 no.4
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• pp.541-550
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• 2015

The purpose of this study was to investigate the effect of lactic acid bacteria (LAB) cell wall extract on the proliferation and cytokine production of immune cells to select suitable probiotics for space food. Ten strains of LAB (Lactobacillus bulgaricus, L. paracasei, L. casei, L. acidophilus, L. plantarum, L. delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pedicoccus pentosaceus) were sub-cultured and further cultured for 3 d to reach 7-10 Log colony-forming units (CFU)/mL prior to cell wall extractions. All LAB cell wall extracts failed to inhibit the proliferation of BALB/c mouse splenocytes or mesenteric lymphocytes. Most LAB cell wall extracts except those of L. plantarum and L. delbrueckii induced the proliferation of both immune cells at tested concentrations. In addition, the production of T_H1 cytokine (IFN-γ) rather than that of T_H2 cytokine (IL-4) was enhanced by LAB cell wall extracts. Of ten LAB extracts, four (from L. acidophilus, L. bulgaricus, L. casei, and S. thermophiles) promoted both cell proliferating and T_H1 cytokine production. These results suggested that these LAB could be used as probiotics to maintain immunity and homeostasis for astronauts in extreme space environment and for general people in normal life.

• Journal of Environmental Health Sciences
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• v.31 no.1
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• pp.94-98
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• 2005

This study was carried out to provide radon concentration and exposure in building. The average radon concentrations of building was measured 1.37 pCi/L in basement, 0.95 pCi/L in 1st layer, 0.74 pCi/L in 2nd layer, 0.56 pCi/L in 3rd layer, and 0.4 pCi/L in 4th layer, respectively. The average radon concentration of basement was measured the higher than any other stairs. Daily average distribution of radon concentrations in building shown that radon concentrations measured in morning at 8hr was the highest value. Monthly average distribution of radon concentrations shown 0.28 ${\pm}$ 0.17 pCi/L in April and 0.82 pCi/L in December that was the highest value. The average concentrations of radon was measured 0.38pCi/L in spring. 0.44 pCi/L in summer, 0.53 pCi/L in autumn, and 0.67 pCi/L in winter, respectively. This result shown that the average concentrations of radon in winter was the higher than any other seasons. That reasons was supposed that effect of number of exchanges and using air conditions was the higher in summer than winter.

• Korean Journal of Ichthyology
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• v.17 no.2
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• pp.91-97
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• 2005

A taxonomic review of the genus Leiognathus was made based on specimens collected from the coast of Korea from 1997 to 2004. The genus Leiognathus in Korea includes 6 species: Leiognathus elongatus, L. fasciatus, L. lineolatus, L. nuchalis, L. rivulatus, and L. bindus. Leiognathus bindus is reported for the first time in Korea. It is characterized by having bright orange spots between the 2nd and 5th spines of the dorsal fin. Detailed morphological measurements and counts and a key to species of Leiognathus from Korea are provided.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.95-99
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• 2003

The leaf segments of garlic (Allium sativum L.) were cultured in vitro and determined optimal concentration of plant growth regulators and sugars for callus induction, multiple shoots regeneration and in vitro bulblet formation. Highest yield of callus was observed in the leaf segment culture on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-D, 30 g/L sucrose and 8 g/L agar. Regeneration rate of multiple shoots from callus was high in the MS medium supplemented with kinetin 3.0 + NAA 3.0 mg/L or SA 1.0 + NAA 3.0 mg/L, containing 30 g/L sucrose. High rate of bulblet formation was observed as the concentration of jasmonic acid increased from 0.5 to 2.0 mg/L in medium, whereas addition of gibberellic acid significantly suppressed bulblet formation. The rate of in vitro bulbing was as high as $96\%$ in MS medium supplemented with 2.0 mg/L jasmonic acid and 120 g/l sucrose after two month culture at $25{\pm}1^{\circ}C$ under 16 hours day light.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.42-44
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• 1998

The effect of medium composition on PLB formation and multiplication and shoot regeneration was studied to establish the micropropagation system of the tropical orchid Phalaenopsis. The highest frequence of PLB formation resulted from the VW medium with 1.2 times ion concentration, 1% sucrose, 1.5g/L PVP or 2.5g/L active charcoal, apple and potato extract and 4g/L gellan gum. The highest ratio of PLB multiplication was obtained from the VW medium with 2% sucrose, apple and potato extract and cotton plate. The shoot regeneration was the most effective with the hyponex medium with 3% apple, 3% potato and 4% banana extract.

• Toxicological Research
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• v.29 no.4
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• pp.293-298
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• 2013

A method of detecting lead was developed using square wave anodic stripping voltammetry (SWASV) with DNA-carbon nanotube paste electrode (CNTPE). The results indicated a sensitive oxidation peak current of lead on the DNA-CNTPE. The curves were obtained within a concentration range of 50 $ngL^{-1}-20mgL^{-1}$ with preconcentration time of 100, 200, and 400 sec at the concentration of $mgL^{-1}$, ${\mu}gL^{-1}$, and $ngL^{-1}$, respectively. The observed relative standard deviation was 0.101% (n = 12) in the lead concentration of 30.0 ${\mu}gL^{-1}$ under optimum conditions. The low detection limit (S/N) was pegged at 8 $ngL^{-1}$ ($2.6{\times}10^{-8}M$). Results showed that the developed method can be used in real-time assay in vivo without requiring any pretreatment and pharmaceutical samples, and food samples, as well as other materials requiring water source contamination analyses.

• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.277-284
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• 2012

In the present study, the effects of plant growth regulators on adventitious shoot and root formation of various explants of $in$ $vitro$ seedlings of $Solanum$ $nigrum$ L. were investigated to determine the optimum conditions for the high-efficiency plant regeneration of this species. The formation of adventitious shoots was higher in leaf explants than in cotyledon, hypocotyl, or epicotyl explants at low concentrations (0.5~2.0 mg $L^{-1}$ ) of 6-benzylaminopurine (BAP). The number of adventitious shoots and the shoot length were also higher in both leaf and cotyledon explants. In particular, 2.0 mg $L^{-1}$ BAP was most effective for stimulating the induction and multiplication of adventitious shoots. In terms of root formation and root development from shoots that were separated from multiple shoots, indole butyric acid (IBA) and indole acetic acid (IAA) were more effective than ${\alpha}$-naphthalene acetic acid (NAA). The percentage of rooting as well as the number of roots per shoot (4.0), root length (7.82 cm), and shoot length (8.76 cm) was highest on MS media supplemented with 0.05 mg $L^{-1}$ IAA. Furthermore, 100% of the regenerated plantlets survived when transplanted to compost soil. These results suggest that leaf explants are the best source for the high-efficiency regeneration of $S.$ $nigrum$ L., and that 2.0 mg $L^{-1}$ BAP and 0.05 mg $L^{-1}$ IAA are the best conditions for shoot and root induction, respectively.

• Journal of the Korean Magnetics Society
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• v.10 no.6
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• pp.269-273
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• 2000

Fe-l7Cr-2M(M=Si, Nb, Mo) alloy powder was prepared by plasma electrode rotating atomizer and than the alloy powder was formed and sintered. The particle shape of the Fe-l7Cr-2M(M=Si, Nb, Mo) alloy power is spherical. The saturation magnetization of the sintered Fe-17Cr-2Mo and Fe-l7Cr-2Nb alloy are 155 emu/g. The saturation magnetization of the sintered Fe-l7Cr-2Si alloy is less than that of the sintered Fe-l7Cr-2Mo and Fe-l7Cr-2Nb alloy. The amplitude relative permeability of the sintered Fe-l7Cr-2M(M=Si, Nb, Mo) alloy has the maximum value in the range of 3∼5 Oe applied field at forming pressure 12 ton/cm$^2$, sintering temperature 1200$^{\circ}C$, and frequency 1 kHz. Power loss of the sintered Fe-l7Cr-2Nb alloy is 40 mW/cc at applied field, H$\sub$a/=5 Oe, and frequency, f=1 kHz. The power loss of the sintered Fe-l7Cr-2Nb alloy is a half of that of the sintered Fe-l7Cr-2Si and Fe-l7Cr-2Mo alloy.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.217-223
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• 2011

This study was conducted to examine the effect of several pretreatments and holding solutions on the vase life and quality of cut Polygonatum odoratum var. pluriflorum for. variegatum stems native to Korea. Postharvest foliar spray of 200 $mg{\cdot}L^{-1}$ $GA_3$ or 200 $mg{\cdot}L^{-1}$ BA + 200 $mg{\cdot}L^{-1}$ $GA_3$ markedly extended the vase life of cut stems of Polygonatum odoratum var. pluriflorum for. variegatum. These treatments maintained high chlorophyll contents as compared with the control (distilled water) during senescence of cut stems. Postharvest pretreatment (dipping) with 100 $mg{\cdot}L^{-1}$ $GA_3$ or 3% sucrose + 200 $mg{\cdot}L^{-1}$ 8-hydroxyquinoline sulfate (HQS) + 100 $mg{\cdot}L^{-1}$ $GA_3$ for 16 hours extended vase life of cut stems 2.5 times as long as nontreated control. Holding solution of 2% sucrose + 200 $mg{\cdot}L^{-1}$ HQS + 100 $mg{\cdot}L^{-1}$ $GA_3$ significantly extended vase life by 3.5 times and increased fresh weight as compared with control. $GA_3$ were very effective on preventing leaf yellowing of cut Polygonatum odoratum var. pluriflorum for. variegatum stems. Postharvest foliar spray of 200 $mg{\cdot}L^{-1}$ $GA_3$ or holding solution of 2% sucrose + 200 $mg{\cdot}L^{-1}$ HQS + 100 $mg{\cdot}L^{-1}$ $GA_3$ markedly extended vase life and improved quality of cut stems of Polygonatum odoratum var. pluriflorum for. variegatum.