• Title/Summary/Keyword: L.D.V

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Effects of Enzyme Treatment on Physicochemical Characteristics of Small Red Bean Percolate (팥 침출액의 이화학적 특성에 대한 효소처리의 영향)

  • Hwang, Cheol-Seung;Jeong, Do-Yeong;Kim, Yong-Suk;Na, Jong-Min;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.37 no.2
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    • pp.189-193
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    • 2005
  • Effect of enzyme treatment on physicochemical characteristics of small tea bean percolate were estimated. Three types of small red bean percolate were prepared by heat treatment for 30 min at $95^{\circ}C$ (1st), 30 min at $95^{\circ}C$ (2nd), and 40 min at $120^{\circ}C$ (3rd). They were then treated with 0.5% ${\alpha}-amylase$ (v/v) for 4 hr at $108^{\circ}C$ (${\alpha}-amylase$ treatment), then with 0.5% ${\beta}-amylase$ (v/v) for 4 hr at $60^{\circ}C$ (${\alpha}-$ and ${\beta}-amylase$ treatment). Crude saponin contents of 1st-3rd percolates were 0.82, 1.44, and 1.52 mg/g, respectively. ${\circ}Brix$ of small red bean percolates treated with enzymes increased to $0.8-1.2\;{\circ}Brix$ with 2nd and 3rd percolates showing no significant difference between ${\alpha}-amylase$ and ${\alpha}-$ and ${\beta}-amylase$ treatments. pH of 3rd percolate treated with ${\alpha}-$ and ${\beta}-amylase$ decreased from initial 6.2 to 4.7. Hunter L value of small red bean percolate treated with ${\alpha}-$ and ${\beta}-$ decreased, whereas a and b values increased. Small red bean beverage made with 3rd percolate showed high score in flavor, taste, and overall acceptability. Results suggest small red bean percolate treated with enzymes could be used for preparation of small red bean beverage.

Synthesis and Properties of Oxygen-bridged Aromatic Polyesters Based on Isomeric Naphthalenediols

  • Park, E-Joon;Park, Bong-Ku;Kim, Jae-Hoon;Lee, Sang-Chul;David J. T. Hill
    • Macromolecular Research
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    • v.8 no.1
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    • pp.12-18
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    • 2000
  • Six aromatic polyesters with ether-linkages were prepared from 4,4'-oxybis(benzoic acid) and naphthalenediol (ND) isomers which were 1,4-, 1,5-, 1,6-, 2,3-, 2,6- and 2,7-derivatives. The solution viscosity numbers ranged from 0.23 to 0.65 dL/g. The glass transition temperatures ranged from 142 to 179$\^{C}$. The initial decomposition temperatures were all above 400$\^{C}$, and the residue weights at 600$\^{C}$ were in the range of 50-64%. Only the polyesters derived from 1,5- and 2,6-NDs, which have a linear linking mode, were found to be semicrystalline and could form thermotropically nematic phase. Multiple melting phenomena and annealing of the polyester derived from 1,5-ND and related polymers are described. The experimental results show that the polyester derived from 1,4-ND of linear shape was amorphous and non-liquid crystalline. Particularly, the polyester derived from 2,3-ND could form a smectic mesophase as banana-shaped molecules, and this is ascribed to the C/sub_2v/ symmetry where highly kinked molecules are packed in the same direction.

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Growth and analysis of Copper oxide nanowire

  • Park, Yeon-Woong;Seong, Nak-Jin;Jung, Hyun-June;Chanda, Anupama;Yoon, Soon-Gil
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.245-245
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    • 2009
  • l-D nanostructured materials have much more attention because of their outstanding properties and wide applicability in device fabrication. Copper oxide(CuO) has been realized as a p-type metal oxide semiconductor with narrow band gap of 1.2 -1.5eV. Copper oxide nanostructures can be synthesized by various growth method such as oxidation reaction, thermal evaporation thermal decomposition, sol-gel. and Mostly CuO nanowire prepared on the Cu substrate such as Copper foil, grid, plate. In this study, CuO NWs were grown by thermal oxidation (at various temperatures in air (1 atm)) of Cu metal deposited on CuO (20nm)/$SiO_2$(250nm)/Si. A 20nm-thick CuO layer was used as an adhesion layer between Cu metal and $SiO_2$

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Carcass Characteristics of Goats Fed Ammoniated Neem (Azadirachta indica) Seed Kernel Cake

  • Anandan, S.;Musalia, L.M.;Sastry, V.R.B.;Agrawal, D.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1451-1454
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    • 2003
  • The present investigation was carried out to study the affect of feeding urea ammoniated neem kernel meal on carcass characteristics and organoleptic properties of the meat in goats. Eight local weaned kids of 3-4 months age with mean body weight of $7.85{\pm}0.42kg$ were assigned to two groups of four each in a completely randomised design and were offered diets containing isonitrogenous concentrate mixtures containing either peanut meal or urea ammoniated neem (Azadirachta indica) seed kernel meal (UANSKM) along with ad libitium oat hay or green sorghum as roughage for 13 fortnights. The animals were slaughtered at the end of the experimental period and the carcass characteristics and organoleptic evaluation of the meat was carried out. The carcass characteristics in terms of dressing percentage, meat bone ratio, percent edible and inedible did not differ between the treatments. Similarly the organoleptic characteristics and cooking loss were comparable between the treatments implying that UANSKM can be substituted for peanut meal in goat diets to alleviate the shortage and high cost of peanut meal without affecting meat quality.

A Study on the Effects of the La Concentration on the Frequency Dependence of Dynamic Pyroelectric Properties of PLT Thin Films (PLT 박막에서 조성에 따른 동적 초전특성의 주파수 의존성에 관한 연구)

  • 차대은;장동훈;강성준;윤영섭
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.10
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    • pp.35-42
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    • 2002
  • Modulation frequency dependences of the pyroelectric properties of PLT (P $b_{1-x}$ L $a_{x}$ $Ti_{1-x}$ 4/ $O_3$) thin films with La concentrations of 5, 10 and 15㏖% have been investigated by using the dynamic method. The PLT thin film with 10㏖% of the La concentration (PLT(10) thin film) shows the most excellent pyroelectric properties among the films. For PLT(10) thin film, the pyroelectric coefficient shows the maximum value of 6.6$\times$10$^{-9}$ C/$\textrm{cm}^2$ㆍK without frequency dependence. The figure of merits for the voltage responsivity and specific detectivity are 1.03$\times$10$^{-11}$ Cㆍcm/J and 1.46$\times$10$^{-9}$ Cㆍcm/J, respectively. Voltage responsivity corresponding to the pyroelectric voltage is almost constant at low modulation frequency and decreases in proportional to frequency at high modulation frequency. Voltage responsivity is 5.15 V/W at 8Hz. Noise equivalent power (NEP) and specific detectivity ( $D^{*}$) of the PLT(10) thin film are 9.93$\times$10$^{-8}$ W/H $z^{1}$2/ and 1.81$\times$10$^{6}$ cmH $z^{1}$2/W at the frequency of 100Hz, respectively. The results indicate that PLT(10) thin film is very suitable for pyroelectric IR sensors.s.s.

Identification of Superior Polyvoltine Hybrids (polyvoltine${\times}$bivoltine) of Silkworm, Bombyx mori L.

  • Rao, C.G.P.;Chandrashekharaiah;Basha, K.Ibrahim;Seshagiri, S.V.;Ramesh, C.;Nagaraju, H.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.43-49
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    • 2004
  • Ten promising polyvoltine mulberry silkworm strains(SDMGl, SDMG2, SDMG3, SDMG4, SDMWl, SDMW2, RMWl, RMW2, RMW3 and RMW4) that are superior in quantitative and qualitative traits have been synthesized in the polyvoltine breeding laboratory of Andhra Pradesh State Sericulture Research & Development Institute, Hindupur through systematic hybridization and appropriate selection methods. After the genotypes were found homozygous for the desired traits, they have been crossed with 3 bivoltine testers $(APS8, APS4 and {NB_2}{D_4})$ and thirty new hybrid combinations were developed for the assessment of their hybrid performance. Phenotypic expressions of economically important quantitative and qualitative traits of fist filial generation were measured and subjected for statistical analysis. Evaluation Index and Subordinate Function methods were employed for the assessment of hybrid performance since they are widely used in silkworm hybrid evaluation. Total of seven poly${\times}$bivoltine combinations, which ranked high in both the methods, were selected as potential combinations for further field test. These combinations also ranked significantly higher than the control hybrid (APMl${\times}$APS8).

Experiment for Clinical Application with Photodiode (Photodiode를 사용한 측정기의 임상응용을 위한 실험)

  • Kim, You-Hyun;Kwon, Soo-Il;Huh, Joon
    • Journal of radiological science and technology
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    • v.19 no.2
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    • pp.71-77
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    • 1996
  • Studies were conducted to determine the clinical application of photodiode. We compared photodiode with ion-chamber as to change of tube potential, tube current, mAs and measured decreasing rate of penetration dose. When tube potential was changed from 60 kVp to 120 kVp, output of photodiode and ion-chamber were changed from 0.4 to 1.625, and 1.018 to 4.268, respectively. This was a good agreement to theory that $I=Kv^2it$(I is intensity, K is constant, v is tube potential, i is tube current, t is time). Characteristics for change of tube current and mAs were also a good agreement to theory. And comparison in decreasing rate of penetration dose was similar except above 6 cm in depth. Our results indicated that photodiode was a good instrument for relative measurement of radiation exposure, but we can not use the photodiode for absolute radiation dose.

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Production and Characterization of Phenylalanine Ammonia-lyase from Rhodotorula aurantiaca K-505

  • Cho, Dae-Haeng;Chae, Hee-Jeong;Kim, Eui-Yong
    • Preventive Nutrition and Food Science
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    • v.2 no.4
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    • pp.354-359
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    • 1997
  • Optimal cultivation conditions for the production of phenylalanine ammonia-lyase(PAL) from Rhodotorula aurantiaca K-505 were selected, and the kinetic parameters of the produced PAL were determined. The most suitable carbon and nitrogen sources were glucose and tryptone, respectively. The strain expressed PAL constituttively when using the optimized semi-complex media. High cell density culture could be critical for maximal production of PAl since the PAL ynthesis was growth associated. maximum PAL activity was observed at initial pH 6.0. although the ll growth was not markedly affected by temperature between 22 and 28$^{\circ}C$, the cells yielded the maximum PAL activity when cultivated at 22$^{\circ}C$. The maximum activity for deamination of L-phenylalnine to trans-cinnamic acid was observed around pH 8.8. The PAL activity gave the maximum at 45$^{\circ}C$, and greatly decreased at higher than 5$0^{\circ}C$. Activation energy({TEX}$E_{a}${/TEX}) calculated from Arrhenius equation was 6.28 kcal/mol in the range of 22$^{\circ}C$ to 4$0^{\circ}C$. A oolf plot showed that the enzyme reaction follows Michaelis-Menten equation, whose {TEX}$K_{M}${/TEX} and {TEX}$V_{max}${/TEX} values were 4.65$\times${TEX}$10^{-3}${/TEX} M and 0.89$\mu$ mol/mg-min respectively.

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Product-Resolved Photodissociations of Iodotoluene Radical Cations

  • Shin, Seung-Koo;Kim, Byung-Joo;Jarek, Russell L.;Han, Seung-Jin
    • Bulletin of the Korean Chemical Society
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    • v.23 no.2
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    • pp.267-270
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    • 2002
  • Photodissociations of o-, m-, and p-iodotoluene radical cations were investigated by using Fourier-transform ion cyclotron resonance (FT-ICR) spectrometry. Iodotoluene radical cations were prepared in an ICR cell by a photoionization charge-transfer method. The time-resolved one-photon dissociation spectra were obtained at 532 nm and the identities of $C_7H_7^+$ products were determined by examining their bimolecular reactivities toward toluene-$d_8$. The two-photon dissociation spectra were also recorded in the wavelength range 615-670 nm. The laser power dependence, the temporal variation, and the identities of $C_7H_7^+$ were examined at 640 nm. The mechanism of unimolecular dissociation of iodotoluene radical cations is elucidated: the lowest barrier rearrangement channel leads exclusively to the formation of the benzyl cation, whereas the direct C-I cleavage channel yields the tolyl cations that rearrange to both benzyl and tropylium cations with dissimilar branching ratios among o-, m-, and p-isomers. With a two-photon energy of 3.87 eV at 640 nm, the direct C-I cleavage channel results in the product branching ratio, [tropylium cation]/[benzyl cation], in descending order, 0.16 for meta >0.09 for ortho >0.05 for para.

Directed evolution을 이용한 (S)-Ketoprofen ethlyester의 광학분활용 Esterase의 특성 개량

  • Kim, Seung-Beom;Kim, Ji-Hui;Yu, Yeon-U
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.445-449
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    • 2003
  • As for the purpose, we first introduce an random mutation into wild-type gene to expand a mutation space, and then further recombine the mutant genes by staggered extension process PCR. As a result, we obtained the best clones 6-52 that showed a high activity and stability, from a round of error prone and staggered extension process PCR. The purified enzyme showed a similar pH stability to the wild-type enzyme and reveal a slightly high optimum pH at 12. In the optimum temperature, an identical dependency was also showed and a quite high stability in the thermal stability was obtained. Along with this, the enzyme was also stable at a reaction that supplement with a 15 % of ethanol as an additive. The addition of other solvents and surfactants did not improve the reaction and thus resulted in a similar profile to those of wild-type enzyme. The specific activity on the target compound rac-ketoprofen ethyl ester was calculated to be about 85, 000 unit, and the kinetic constants Km and Vmax were determined to be 0.2 mM and 90 mM/mg-protein/min respectively. The deduced amino acid alignment with the wild type enzyme revealed five mutations at L120P, I208V, T249A, D287H and T357A. Based on these observations, the site directed mutagenesis to delineate the mutagenic effect is under progress.

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