• 한국항해항만학회지
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• 제28권3호
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• pp.213-219
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• 2004

본 논문에서는 1,452∼l,492 MHz L-Band 대역의 위성 DAB 수신기를 위한 저잡음증폭기를 입ㆍ출력 반사계수와 전압정재파비를 개선하기 위하여 평형증폭기 형태로 설계 및 제작하였다. 저 잡음증폭기는 GaAs FET소자인 ATF-10136을 사용한 저 잡음증폭단과 MMIC 소자인 VNA-25을 사용한 이득증폭단을 하이브리드 방식으로 구성하였으며, 최적의 바이어스를 인가하기 위하여 능동 바이어스 회로를 사용하였다. 적용된 능동 바이어스 회로는 소자의 펀치오프전압($V_P$)과 포화드래인 전류($I_{DSS}$)의 변화에 따라 주어진 바이어스 조건을 만족시키기 위해 소스 저항과 드래인 저항의 조절이 필요없다. 즉, 능동 바이어스 회로는 요구된 드래인 전류와 전압을 공급하기 위해 게이트-소스 전압($V_{gs}$)을 자동적으로 조절한다. 저잡음증폭기는 바이어스 회로와 RF 회로를 FR-4기판 위에 제작하였고, 알류미늄 기구물에 장착하였다. 제작된 저잡음증폭기는 이득 32 dB, 이득평탄도 0.2 dB, 0,95 dB 이하의 잡음지수, 입ㆍ출력 전압정재파비는 각각 1.28, 1.43이고, $P_{1dB}$ 는 13 dBm으로 측정되었다.

• Bulletin of the Korean Chemical Society
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• 제18권9호
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• pp.922-928
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• 1997

The conformational transition of poly(L-proline) (PLP), Form Ⅱ → Form Ⅰ and the intermolecular aggregation of the product, Form Ⅰ, during and after the transition in water-propanol (1:7, 1:9, 1:15.7, and 1:29 v/v) were studied. For the study, the viscosity change and excess light scattering intensity were measured in the course of the transition which was determined by the Form Ⅰ fraction, fI of the sample solution. For the PLP sample of molecular weight Mv=31,000 the experimental results show that the reaction course is roughly divided into three regions: in the first region [fI=0.27 to 0.40 (- [α]D=400 to 330)], the conformational change of Form Ⅱ → Form Ⅰ occurs with decrease of viscosity, in the second region [fI=0.40 to 0.80 (- [α]D=330 to 120)], a partial side-by-side (p-S-S) type aggregation in which Form Ⅰ blocks interact with each other, which induces the increase of viscosity, starts to occur, and in the third region [fI=0.80 to 1.00 (- [α]D=120 to 15)], a side-by-side type (raft like) aggregation of Form Ⅰ or an end-to-end (E-E) type aggregation occurs according to the solvent situation, i.e., in a water-rich medium [water-propanol (1:9 or 1:7 v/v)], the (S-S) type aggregation with a gross decrease in viscosity occurs while in a water-poor medium [water-propanol (1:29 or 1:15.7 v/v), the (E-E) type aggregation with a large increase in viscosity occurs. The (S-S) type aggregation was promoted at high temperatures. Based on the structure of PLP, a reasonable mechanism for the (p-S-S) and (S-S) aggregation which occurs with the transition of Form Ⅱ → Form Ⅰ is considered. The suggested mechanism was also supported by the result of chain length effect of PLP for the aggregation.

• Translational and Clinical Pharmacology
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• 제26권3호
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• pp.134-140
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• 2018

This study aimed to develop a UPLC-MS/MS method for determining plasma levels of L-aspartic acid and L-asparagine and the activity of L-asparaginase. L-aspartic acid, L-asparagine, and L-aspartic acid-2,3,3-$d_3$ were extracted from human plasma by protein precipitation with sulfosalicylic acid (30%, v/v). The plasma samples were analyzed using an Imtakt Intrada amino acid analysis column with 25 mM ammonium formate and 0.5% formic acid in acetonitrile as the mobile phase with step gradient method at a flow rate of 0.5 mL/min. The injection volume was $5{\mu}L$, and the total run time was 15 min. Inter- and intra-batch accuracies (%) ranged from 96.62-106.0% for L-aspartic acid and 89.85-104.8%, for L-asparagine, and the coefficient of variation (CV%) did not exceed 7%. The validation results for L-aspartic acid and L-asparagine satisfied the specified criterion, however, the results for L-asparaginase activity assay showed a borderline validity. This study could be a foundation for further development of therapeutic drug monitoring systems using UPLC-MS/MS.

• Animal Bioscience
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• 제37권6호
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• pp.965-981
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• 2024

Objective: Milk composition varies considerably and depends on paratypical, genetic, and epigenetic factors. MiRNAs belong to the class of small non-coding RNAs; they are one of the key tools of epigenetic control because of their ability to regulate gene expression at the post-transcriptional level. We compared the relative expression levels of miR-106b, miR-191, and miR-30d in milk to demonstrate the relationship between the content of these miRNAs with protein and fat components of milk in Holstein and Ayrshire cattle. Methods: Milk fat, protein, and casein contents were determined in the obtained samples, as well as the content of the main fatty acids (g/100 g milk), including: saturated acids, such as myristic (C14:0), palmitic (C16:0), and stearic (C18:0) acids; monounsaturated acids, including oleic (C18:1) acid; as well as long-, medium- and short-chain, polyunsaturated, and trans fatty acids. Real-time stem-loop one-tube reverse transcription polymerase chain reaction with TaqMan probes was used to measure the miRNA expression levels. Results: The miRNA expression levels in milk samples were found to be decreased in the first two months in Holstein breed, and in the first four months in Ayrshire breed. Correlation analysis did not reveal any dependence between changes in the expression level of miRNA and milk fat content, but showed a multidirectional relationship with individual milk fatty acids. Positive associations between the expression levels of miR-106b and miR-30d and protein and casein content were found in the Ayrshire breed. Receiver operating characteristic curve analysis showed that miR-106b and miR-30d expression levels can cause changes in fatty acid and protein composition of milk in Ayrshire cows, whereas miR-106b expression level determines the fatty acid composition in Holsteins. Conclusion: The data obtained in this study showed that miR-106b, miR-191, and miR-30d expression levels in milk samples have peculiarities associated with breed affiliation and the lactation period.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2003년도 International Meeting on Information Display
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• pp.673-674
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• 2003

The reliability of TFT, crystallized by sequential lateral solidification (SLS) technology, has been studied High current damage is characterized by high gate bias (-20V) and drain bias (-10V). It is found that performance of SLS TFTs is enhanced by high current stress up to 300 sec of stress time for 20/8 (W/L) N-TFT. After that, TFT performance is degraded with the increase of the stress time. It is speculated from the experimental data that SLS TFTs initially contain a number of unstable defect states. Then, the defect states seem to be cured by high current stress.

• 약학회지
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• 제52권4호
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• pp.241-251
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• 2008

In this study, isolation of antioxidative compounds was performed for development of anti-oxidizing agent. $CHCl_{3}$, $H_{2}O$, 30%, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH method, TBARS assay, and SOD like activity. It was revealed that 30%, 60% MeOH fractions had significant antioxidative activity. From 30%, 60% MeOH fraction, nine compounds were isolated and elucidated kaempferol $3-O-{\alpha}-L-rhamnopyranosyl$ $(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (1), quercetin $7-O-{\beta}-D-glucopyranoside$ (II), quercetin $3-O-{\alpha}-L-rhamnopyranosyl$ $(1{\rightarrow}6)$ ${\beta}-D-glucopyranoside(rutin)$ (III), 6-hydroxykaempferol $3-O-{\beta}-D-glucopyranoside$ (lV), kaempferol $3-O-{\beta}-D-glucopyranosyl$ $(1{\rightarrow}2)$ ${\beta}-D-glucopyranoside$ (V), kaempferol $3-O-{\beta}-D-glucopyranoside$ (VI), luteolin (VII), quercetin $3-O-{\beta}-D-glucopyranoside$ (VIII), apigenin $7-O-{\beta}-D-glucuronopyranoside$ (IX) through physicochemical data and spectroscopic methods (Negative FAB-MS, $^1H-NMR$, $^{13}C-NMR$). Entirely, all compounds had similar antioxidative activity, but more OH group had more antioxidative activity.

• 전자공학회논문지D
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• 제35D권2호
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• pp.52-59
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• 1998

The ZnS:Mn thin film electroluminescent(TFEL) devices fabricated by ALE system were investigated. Yellow-orange light emission was observed when the applied voltage exceeded 134 V and luminance increased sharply as the applied voltage increased. Luminance of 568 Cd/c $m^{2}$ was obtained under 1 KHz sinusoidal voltage wave application at the peak applied voltage of 230 V. The peak wavelength of the emissionwas 577 nm. The C-V, Q-V, $Q_{t}$ - $F_{p}$ , L- $Q_{cond}$, and V- $Q_{pol}$ have been measured under theapplication of the trapezoidal wave with its pulse width varying 0 to 75 .mu.sec. The phoshor and the insulator capacitance of the TFEL device under test were 24.3 nF/c $m^{2}$ and 9 nF/c $m^{2}$, respectively. It was observed that the threshold voltage changed from 137V to 100V as the pulse width varied from 0 to 75 .mu.sec. The L- $Q_{cond}$ characteristics showed that the light emission increased in proportion to the $Q_{cond}$. The luminance increased from 386 Cd/ $m^{2}$ to 607 Cd/ $m^{2}$ when the $Q^{+}$$_{cond}$ increased from 1.3 .mu.C/c $m^{2}$ to 2.3 .mu.C/c $m^{2}$. The V- $Q_{pol}$ characteristics showed that the V was inversely proportional to $Q_{pol}$./. th/ was inversely proportional to $Q_{pol}$./. pol/./.

• The Korean Journal of Ecology
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• 제20권1호
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• pp.43-49
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• 1997

Effects of addition of soil ameliorants on the growth and nutrient absorption of Zea mays, Miscanthus sinensis and Phytolacca americana were investigated. Acid soil from Yeocheon Industrial Complex were used for the control plot (AS). We made two treatment plots, acid soil + lime (AS + L) and acid soil + dolomite (AS+D). Ration of acid soil : soil ameliorants in freatment plot was 50 : 1(V:V). Acid soil and soil ameliorants were mixed thoroughly before use. Shoot length of corn in AS+L and AS+D was considerably increased by 141% and 137%, respectively, compared with that in AS. Pokeweed in AS+L and AS+D also increased by 183% and 152%, respectively, compared with that in AS. However, growth of Miscanthus sinensis showed slight difference between the control and the treatment plots. Biomass of corn and porkweed in the treatment plots were also greater than those in the control plots. During the growth experiment with corn, concentrations of Ca and Mg in soil were increased and A1 decreased with increased soil pH in the treatment plots. Amount of aluminum absorbed by corn in the control plot was greater than that in the treatment plots. In case of Miscanthus sinensis, however, aluminum absorption in the control plot was lower than those in the treatment plots.

• 전기전자학회논문지
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• 제19권3호
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• pp.335-341
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• 2015

최근 들어 철강의 두께 제어를 위해 고정밀도의 절대 위치 검출 센서에 대한 필요성은 증가하고 있다. 열악한 공장 환경 하에서 직선 운동하는 실린더의 절대 위치를 측정하기 위해서는 LVDT가 널리 사용된다. 본 논문에서는 1 미크론의 고해상도를 가지는 3상 LVDT 및 L/D (LVDT to digital) 변환기를 구현하였다. 이를 위하여 첫째 U, V, W 3상 정현파에 대한 시그널링을 FPGA로 설계하였다. 둘째 위치 정보에 대한 A상, B상 펄스 파형 출력 알고리즘을 구현하였다. 마지막으로 성능 평가를 위하여 기존의 센서들과 비교하였다. 실험 결과 직선성 편이 오차는 0.009788 [mm] 로써 2.2% 향상된 결과를 얻었고 정현파의 왜곡률은 평균 0.0751%로 기존보다 33%의 향상된 결과를 얻어 우수한 센서임을 입증하였다.

• 분석과학
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• 제37권4호
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• pp.211-219
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• 2024

This study presents a sensitive and reliable method for determining tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC) residues in shrimp samples. A two-step process involving liquid-liquid extraction (LLE) followed by solid-phase extraction (SPE) was developed prior to HPLC analysis. The target analytes were effectively extracted using EDTA/McIlvaine buffer (pH 4.0): methanol (80:20, %v/v), with subsequent clean-up using a C18 SPE cartridge. HPLC separation was conducted on a C18 column (250 mm × 4.6 mm i.d., 5 ㎛) at 30 ℃, using 0.01 % trifluoroacetic acid (A) and acetonitrile (B) as the mobile phase. A gradient elution protocol was applied, transitioning from 85(A):15(B) %v/v to 70(A):30(B) %v/v at 7 min, with a 5 min hold, followed by adjustment to 85(A):15(B) %v/v for 13-14 min. The detection was performed using photodiode array (PDA) at 365 nm with a flow rate of 1.0 mL/min. The calibration curves exhibited good linearity within a concentration range of 0.4-6.0 ㎍/mL (R² > 0.995). The limits of detection (LOD) for TC, OTC, and CTC in shrimp were 0.034, 0.029, and 0.021 ㎍/mL, respectively. The limits of quantitation (LOQ) for TC, OTC, and CTC were found to be 0.114, 0.097, and 0.071 ㎍/mL, respectively. Recoveries of TC, OTC, and CTC from spiked shrimp samples ranged from 91.0 % to 95.5 %, 92.4 % to 97.2 %, and 93.3 % to 96.6 %, respectively. This method was successfully applied to the determination of TC, OTC, and CTC residues in shrimp samples sourced from various local markets.