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Nematicidal Activity of Kojic Acid Produced by Aspergillus oryzae against Meloidogyne incognita

  • Kim, Tae Yoon;Jang, Ja Yeong;Jeon, Sun Jeong;Lee, Hye Won;Bae, Chang-Hwan;Yeo, Joo Hong;Lee, Hyang Burm;Kim, In Seon;Park, Hae Woong;Kim, Jin-Cheol
    • Journal of Microbiology and Biotechnology
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    • v.26 no.8
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    • pp.1383-1391
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    • 2016
  • The fungal strain EML-DML3PNa1 isolated from leaf of white dogwood (Cornus alba L.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. The active fungal strain was identified as Aspergillus oryzae, which belongs to section Flavi, based on the morphological characteristics and sequence analysis of the ITS rDNA, calmodulin (CaM), and β-tubulin (BenA) genes. The strain reduced the pH value to 5.62 after 7 days of incubation. Organic acid analysis revealed the presence of citric acid (515.0 mg/kg), malic acid (506.6 mg/kg), and fumaric acid (21.7 mg/kg). The three organic acids showed moderate nematicidal activities, but the mixture of citric acid, malic acid, and fumaric acid did not exhibit the full nematicidal activity of the culture filtrate of EML- DML3PNa1. Bioassay-guided fractionation coupled with 1H- and 13C-NMR and EI-MS analyses led to identification of kojic acid as the major nematicidal metabolite. Kojic acid exhibited dose-dependent mortality and inhibited the hatchability of M. incognita, showing EC50 values of 195.2 μg/ml and 238.3 μg/ml, respectively, at 72 h post-exposure. These results suggest that A. oryzae EML-DML3PNa1 and kojic acid have potential as a biological control agent against M. incognita.

A Study of Mode of Action of Alachlor III. Effect of Alachlor on Cell Division, Cell Kinetics, Cell Elongation, and Cell Differentiation in Oat (Avena sativa L.) (Alachlor의 제초기구(除草機構)에 관(關)한 연구(硏究) III. Alachlor가 귀리의 세포분열(細胞分裂), Cell kinetics, 세포신장(細胞伸長) 및 분화(分化)에 미치는 영향(影響))

  • Kwon, S.W.;Kim, J.C.
    • Korean Journal of Weed Science
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    • v.12 no.2
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    • pp.102-109
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    • 1992
  • There was significant reduction in the mitotic indices of oat roots treated with alachlor. Uniform decrease in prophase, metaphase, anaphase, and telophase as treatment time increasing was observed. Alachlor did not disrupt mitosis, but rather inhibited the onset of mitosis. Labeled dividing cells were significantly inhibited, but the number of labeled interphase cells of all treatment were increased, as compared with control in 8 hr and 12hr period. Labeled dividing cells which entered mitosis thru $G_2$ were inhibited approximately 68% at 8hr after treatment with $1{\times}10^{-5}$ M of alachlor. Alachlor apparently inhibited from the $G_2$stage into mitosis of dividing cells. After 24 hr treatment, 12.1% abd 46.6% inhibition of coleoptile growth occurred at $1{\times}10^{-5}$ M and $1{\times}10^{-4}$ M, respectively. Cell elongation was inhibited by alachlor but was less sensitive than cell division. The longitudinal section cells of oat roots treated with $1{\times}10^{-4}$ M alachlor for 12 hr were observed to be enlarged central cylinder and also showed degradation of apical meristem zone, as compared with the untreated roots.

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Application Examples of Daecheong Dam for Efficient Water Management Based on Integrated Water Management (통합물관리 기반 효율적 물관리를 위한 대청댐 실무적용 사례)

  • Kang, Kwon-Su;Heo, Jun-Haeng
    • Proceedings of the Korea Water Resources Association Conference
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    • 2017.05a
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    • pp.85-85
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    • 2017
  • 효율적 물관리란 거대한 물순환 과정에서 인간이 편안한 삶을 사는데 필요한 물의 이용효율을 극대화하는 것이다. 과거의 물관리는 이원화된 수량과 수질관리, 수량중심에서는 용수공급과 홍수조절이 주요한 관심사였다. 현재는 과거의 물관리에 친수와 환경을 더한 복잡한 분야로 확대되고 있다. 통합물관리란 물을 최적으로 관리하기 위해 물관리 이해당사자간의 소통과 물 기술의 고도화를 기반으로 기존에 분산된 물관리 구성요소들(시설 정보, 수량 수질 등)을 권역적으로 관리하는 것을 말한다. 본 연구에서는 대청댐 방류에 따른 금강 하류부의 홍수추적을 위해 수행한 댐하류 소유역별 강우량 빈도분석 과정, 용담댐 방류를 고려한 대청댐 홍수도달시간 검토, Poincare Section과 신경망기법을 이용한 수문자료 예측, 추계학적 다변량 해석과 다변량 신경망해석에 의한 대청댐 유입량 산정과정, 보조여수로 건설에 따른 주여수로와 보조여수로간의 연계운영방안, 단계(관심, 주의, 경계, 심각)를 고려한 대청댐 확보수위 산정, 저수지 중장기 운영계획 수립과 댐 운영 기준수위를 결정하기 위해 누가차분방식으로 적용되는 갈수기 유입량 빈도분석에 대한 실무적용 사례를 소개하고자 한다. 강우량 빈도분석 과정은 L-모멘트방법(Hosking과 Wallis, 1993)을 적용하였고, 홍수도달시간 검토는 평균유속, 하류 수위상승 기점 영향검토, 수리학적 모형(FLDWAV, Progressive lag method 등)을 활용하였다. 카오스 이론을 도입하여 대청댐 수문자료의 상관성 검토 및 추계학적 모형을 이용한 모의발생을 유도하여 수문자료 예측을 시행하였다. 추계학적 모형과 신경망모형 연구의 대상은 대청댐으로, 시계열 자료는 댐의 월강우량, 월유입량, 최고기온, 평균기온, 최소기온, 습도, 증발량 등의 자료를 기반으로 하였다. 적용기간은 1981~2009년의 자료를 이용하여 2010년 1월부터 12월까지 12개월 동안의 월유입량을 예측하였다. 수문자료 해석의 기본이 되는 약 30년간의 자료를 이용하여 분석을 실시하였다. 대청댐의 유입량 예측을 위해 적용된 모형으로는 추계학적 모형인 ARMA모형, TF모형, TFN 모형 등이 적용되었고, 또한 신경망 모형의 종류인 다층 퍼셉트론, PCA모형 등을 활용하여 실측치와 가장 가깝게 근사화시키는 방법론을 찾고자 하였다. 또한, 기존여수로와 보조여수로 연계운영을 위해 3차원 수치해석을 통한 댐하류 안정성 검토 및 확보수위 산정을 통해 단계(관심, 주의, 경계, 심각)별로 대처가 가능한 수위를 산정하였다.

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A New Pod Edible Kidney Bean Variety "Hwanghyeob 1" with Yellow Pod Color (협채용 강낭콩 신품종 "황협1호")

  • Shin, Doo-Chull;Kang, Sung-Taek;Han, Won-Young;Baek, In-Youl;Chung, Myoung-Gun;Park, Keum-Yong;Kang, Nam-Suk;Hwang, Young-Hyun;Ko, Jong-Min;Suh, Duck-Yong;Kim, Ho-Yeong
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.466-469
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    • 2008
  • A new kidney bean cultivar, "Hwanghyeob 1" was developed at the National Institute of Crop Science in 2005. "Hwanghyeob 1" was selected from a cross between KLG50064 and KLG50073. It has determinate growth habit, white flower, yellow pod color, oval shape of crossed section of pod at the harvesting time for edible pod, white seed coat and middle seed size (21.7 grams per 100 seeds). The average yield of edible pod of "Hwanghyeob 1" was 23.55 M/T per hectare in the yield trials which was carried out at the green house in spring and autumn in 2005. This yield level was 4 percent higher than that of the check cultivar "Kangnangkong 1".

A New Pod Edible Kidney Bean Variety "Hwanghyeob 2" with Yellow Pod Color (협채용 강낭콩 신품종 "황협2호")

  • Shin, Doo-Chull;Kang, Sung-Taek;Han, Won-Young;Baek, In-Youl;Choung, Myoung-Gun;Park, Keum-Yong;Ha, Tae-Jung;Hwang, Young-Hyun;Ko, Jong-Min;Suh, Duck-Yong;Kim, Ho-Yeong
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.470-473
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    • 2008
  • A new kidney bean cultivar, "Hwanghyeob 2" was developed for edible pod kidney bean adaptable to Korean cultivation at the National Institute of Crop Science in 2005. "Hwanghyeob 2" was selected from a cross between KLG50073 and KLG50072. It has determinate growth habit, white flower, yellow pod color, oval shape of crossed section of pod at the harvesting time for edible pod. It has white seed coat and middle seed size (20.4 grams per 100 seeds). The average yield of edible pod of "Hwanghyeob 2" was 22.43 M/T per hectare in the yield trials which was carried out at the green house in spring and autumn in 2005. This yield level was 11 percent higher than that of the check cultivar "Kangnangkong 1".

Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris

  • Lee, Mi Rim;Bae, Su Ji;Kim, Ji Eun;Song, Bo Ram;Choi, Jun Young;Park, Jin Ju;Park, Ji Won;Kang, Mi Ju;Choi, Hyeon Jun;Choi, Young Whan;Kim, Kyung Mi;Hwang, Dae Youn
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.288-294
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    • 2018
  • A few clues about correlation between endoplasmic reticulum (ER) stress and mulberry (Morus alba) leaves were investigated in only the experimental autoimmune myocarditis and streptozotocin-induced diabetes. To investigate whether a novel extract of mulberry leaves fermented with Cordyceps militaris (EMfC) could suppress ER in fatty liver, alterations in the key parameters for ER stress response were measured in high fat diet (HFD)-induced obese C57L/6 mice treated with EMfC for 12 weeks. The area of adipocytes in the liver section were significantly decreased in the HFD+EMfC treated group as compared to the HFD+Vehicle treated group, while their level was higher in HFD+Vehicle treated group than No treated group. The level of the eukaryotic initiation factor 2 alpha ($eIF2{\alpha}$) and inositol-requiring enzyme 1 beta ($IRE1{\alpha}$) phosphorylation and CCAAT-enhancer-binding protein homologous protein (CHOP) expression were remarkably enhanced in the HFD+Vehicle treated group. However, their levels were restored in the HFD+EMfC treated group, although some differences were detected in the decrease rate. Similar recovery was observed on the ER stress-induced apoptosis. The level of Caspase-3, Bcl-2 and Bax were decreased in the HFD+EMfC and HFD+orlistat (OT) treated group compared to the HFD+Vehicle treated group. The results of the present study therefore provide first evidence that EMfC with the anti-obesity effects can be suppressed ER stress and ER stress-induced apoptosis in the hepatic steatosis of HFD-induced obesity model.

Antioxidant Activity of Jeolpyun Containing Chaenomeles sinensis Koehne Powder (모과분말을 첨가한 절편의 항산화활성)

  • Lee, Na Gyeom
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.6
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    • pp.413-419
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    • 2022
  • The recent various ingredients are used in the food industry. Among them the results of moisture content, Hunter color system, and antioxidant activity by making a section different Jeolpyun from the general Chaenomeles sinensis Koehne and Chaenomeles sinensis Koehne powder, which have a bitter and sour taste as follows. The moisture content of the Chaenomeles sinensis Koehne was 73.8% and the moisture content were Chaenomeles sinensis Koehne powder 10.2%. The fat content of Chaenomeles sinensis Koehne powder increased by 0.9% and the ash content also increased by 0.9%. As the result of the moisture content was reduced to 60.15~41.03% and values of Hunter color system, the L-values of the Chaenomeles sinensis Koehne powder Jeolpyeon significantly decreased(p<0.05). The a-values also significantly increased(p<0.05) and the b-values was the highest 35.94 for the 3 % Chaenomeles sinensis Koehne powder. There were significant Levels of total phenolics and TEAC found for the antioxidant activity of the jeolpyeon samples(1~7%) with Chaenomeles sinensis Koehne Powder added(p<0.05). We have the above results adding Chaenomeles sinensis Koehne powder to make a traditional rice cake will help to use it as basic data for making rice cakes with excellent antioxidant activity.

Occurrence of an invertase producing strain of Aspergillus niger LP5 isolated from longan pollen and its application in longan syrup production to feed honey bees (Apis mellifera L.)

  • Danmek, Khanchai;Ruenwai, Rawisara;Sorachakula, Choke;Jung, Chuleui;Chuttong, Bajaree
    • Journal of Ecology and Environment
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    • v.46 no.2
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    • pp.136-143
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    • 2022
  • Background: In northern Thailand, the longan flower is the principal nectar source for honey production. Microorganisms play a critical function in the agricultural ecology. The morphological characteristics of fungal species found in longan pollen were studied. Aspergillus spp. were found to be invertase-producing strains and were employed in the longan syrup production process. The purpose of this study was to evaluate the effects of invertase-added longan syrup on the adult honey bee population numbers that were fed by this syrup for 16 weeks. Results: Different fungal species were found in longan pollen samples. Aspergillus was the main genus, with three predominant sections: Nigri, Flavi, and Terrei. Other isolated species were Trichoderma spp., Rhizopus spp., Neurospora spp., Chaetomium spp., Fusarium spp. and Penicillium spp. However, Aspergillus spp. is the only fungal species that produces the enzyme invertase. The invertase-producing strains belonging to the Aspergillus section Nigri were found to be A. niger LP5 with an optimum activity at pH 6.0 and 60℃. When A. niger LP5 invertase was used for longan syrup processing, the highest levels of glucose (3.45%) and fructose (2.08%) were found in invertase added longan syrup (C), while fresh (A) and boiled longan syrup (B) had lower contents of both sugars. The sucrose content was detected in (A) at 4.25%, while (B) and (C) were at 4.02% and 3.08%, respectively. An appropriate amount of sugar to feed and maintain the honey bee population was considered. The data showed no statistically significant differences between the two selected forms of longan syrup compared to the sugar syrup examined by the adult honey bee population. Conclusions: The main species of isolated fungi from longan pollen were Aspergillus spp. The discovery of an invertase-producing strain of A. niger LP5 has enabled its application for enzyme utilization in the invert sugar preparation process. The adult worker bee populations fed by longan syrup from both boiled and invertase-added sources showed an increasing trend. Artificial syrup made from longan fruit to feed honey bees when natural food sources are limited can be applied.

Application of AFLPs to Phylogenetic Analysis of Aegilops (AFLPs에 의한 Aegilops의 계통발생학적 재평가)

  • Park, Yong-Jin;Shim, Jae-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.790-799
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    • 1997
  • Aegilops genus is known to include the donor species of the Band D genome of the bread wheat(ABD). An effort to establish a better strategy for phylogenetic relationships about Aegilops polyploids by AFLPs(Amplified Fragment Length Polymorphisms) was conducted using the 19 Aegilops sPP. and T. aestivum. The 207 polymorphic bands from the amplified products on the 6% acrylamide denaturing sequencing gels were obtained with the 7 AFLP primer combinations, and used to account for the genetic similarities and cluster analysis using NTSYS program. According to the genome analysis, the $M^h$-genome of Ae. heldreichii was estimated as an intermediate genome between the M-genome of Ae. comosa and N-genome of Ae. uniaristata and supposed to be incorporated in the establishing process of UM-genome as a possible diploid donor. And Ae. ventricosa(DN) was more close to Ae. umbellulata(U) than Ae. squarrosa(D). The close relationship between Ae. squarrosa and T. aestivum was perceived as a diploid donor of D-genome. As for the polyploid species, hexaploid Ae. triaristata was more closely related to Ae. columnaris rather than tetraploid Ae. triaristata. The clustered groups were, basically same to the previous Gihara's sections based on phenotypes and pairing analysis of interspecific hybrids. AFLP was evaluated as an efficient and powerful method in the genome evaluation of closely related species.

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Analysis of the road surface runoff at the continuous section (연속구간 도로의 표면흐름 분석)

  • Kim, Jung Soo;Jo, Jun Beom;Kim, Soo Youl;Lee, Sung Ho
    • Proceedings of the Korea Water Resources Association Conference
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    • 2020.06a
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    • pp.242-242
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    • 2020
  • 도시화와 산업화에 따른 유출환경의 변화로 인해 도심지에서 발생하는 대부분의 유출은 도로표면을 따라 이동하고 도로변에 설치된 빗물받이에 의해 배수된다. 이 때 빗물이 원활하게 배수되지 않아 노면수가 정체되고, 이 노면수가 인근 주택지로 유입되어 침수 피해가 발생한다. 이러한 현상은 유출량이 집중되는 도심지 저지대에서 주로 발생하며 설계 한도를 초과하지 않는 정상적인 강우 조건에서도 빈번히 나타나고 있다. 이는 최근의 기후 변화로 인한 집중 호우 등을 고려하여 설계 기준이 지속적으로 조정되어야 함과 동시에 도로 노면수의 배수 능력 평가를 위한 실제적인 연구가 이루어져야 한다는 필요성을 나타내고 있다. 특히 도로 빗물받이의 효율은 주로 종경사 및 횡경사 등의 도로 조건, 표면 유출 우수량, 빗물받이의 형상 등에 의존한다. 그러므로 상향된 설계빈도 및 도로 조건을 고려한 도로 표면 유출에 대한 흐름 분석과 빗물받이 특성을 고려한 실증연구의 분석 및 설계 해석 알고리즘의 개발이 시급한 실정이다. 본 연구에서는 도로 노면수의 배수 능력 증대를 위해 연속 설치된 빗물받이 조건 및 도로 경사조건을 고려하여 도로 배수시설의 표면 유출 모델 설계 알고리즘을 개발하였다. 표면 유출 유량은 도로의 차선(2~4차선), 경사(종경사 2~10% 및 횡경사 2~10%) 및 설계빈도(최대 30년)를 고려하였으며 유량이 노면 진행방향을 따라 연속적으로 증가하는 부등류 흐름 해석 방법을 채택하였다. 또한, 빗물받이 설치간격(L)과 도로의 폭(W)의 곱으로 계산되는 단순 직사각형 형태의 노면 형상이 아닌 도로 종경사 및 횡경사에 따른 유달거리를 산정하여 평행사변형 형태의 노면 형상을 계산하고 이에 따른 면적(A), 도달시간(tc) 및 강우강도(I)를 산정하였다. 이와 같은 1차원 흐름분석을 통해 도로 표면흐름 및 빗물받이 설치 간격을 제시하고 기존의 제시된 빗물받이 설치 간격과 비교하여 본 연구에서 제시한 설계 알고리즘을 검증하였다. 또한, 수리 실험을 통해 측정된 빗물받이 차집효율을 이용하여 연속 구간에서의 도로 표면 유출 모델의 적용성을 제시하였다. 이는 빗물받이 유입구를 통해 모든 유량이 유출된다고 가정하고 단독 구간에서의 수리분석 결과만을 고려하여 이를 확장시켜 개발된 기존의 도로 표면 유출 모델을 보완할 수 있을 것으로 기대된다. 본 연구에서 제시하고 있는 설계 알고리즘을 적용한다면 도로 전체 구간에서의 흐름 변화 분석과 배수효율의 정량적인 분석이 가능하므로 향후 도로 표면 침수 피해 저감 및 배수능력 증대를 위한 실증적이고 정량적인 분석 방법이 될 것으로 판단된다.

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