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Affinity Filtration Chromatography of Proteins by Chitosan and Chitin Membranes: 1. Preparation and Characterization of Porous Affinity Membranes (키토산 및 키틴 막에 의한 단백질의 친화 여과 크로마토그래피: 1. 다공성 친화 막의 제조와 특성 평가)

  • Youm Kyung-Ho;Yuk Yeong-Jae
    • Membrane Journal
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    • v.16 no.1
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    • pp.39-50
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    • 2006
  • Porous chitosan and chitin membranes were prepared by using silica particles as porogen. Membrane preparation was achieved via the following three steps: (1) chitosan film formation by casting an chitosan solution containing silica particles, (2) preparation of porous chitosan membrane by dissolving the silica particles by immersing the film into an alkaline solution and (3) preparation of porous chitin membrane by acetylation of chitosan membrane with acetic anhydride. The optimum preparation conditions which could provide a chitosan and chitin membranes with good mechanical strength and adequate pure water flux were determined. To allow protein affinity, a reactive dye (Cibacron Blue 3GA) was immobilized on porous chitosan membrane. Binding capacities of affinity chitosan and chitin membranes for protein and enzyme were determined by the batch adsorption experiments of BSA protein and lysozyme enzyme. The maximum binding capacity of affinity chitosan membrane for BSA protein is about 22 mg/mL, and that of affinity chitin membrane for lysozyme enzyme is about 26 mg/mL. Those binding capacities are about $several{\sim}several$ tens times larger than those of chitosan and chitin-based hydrogel beads. Those results suggest that the porous chitosan and chitin membranes are suitable in affinity filtration chromatography for large scale separation of proteins.

An experiment of the particle deposition on a circular cylinder in a laminar flow (원관 주위 유하 액막에 의한 관 외벽에서의 입자 부착에 대한 실험)

  • 정종수;이윤표;정기만;박찬우
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.12 no.2
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    • pp.113-119
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    • 2000
  • An experimental study has been carried out in order to investigate on a particle deposition on a circular cylinder surface. The present study is focused on the particulate fouling occurring in a heat exchanger for a seawater desalinization, in a laminar flow over circular cylindrical tubes. The objective is to investigate how NaCl concentration influences the $SiO2$ particle deposition on the surface of a glass circular cylinder. The NaCl concentration was changed from 0 g/L to 40 g/L. As the experimental results of $SiO2$ particle which is deposited on the glass circular cylinder surface showed, particle deposition rate per unit time increases rapidly with the increase of NaCl concentration between 0 g/L and 15 g/L. After the maximum of particle deposition rate was found at the NaCl concentration of 15 g/L, particle deposition rate remains unchanged or decreases gradually with the NaCl concentration from 15 g/L to 40 g/L. Also the $SiO2$ deposition rate of particles does not have serious variations with the position at present glass surface.

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DETERMINATION OF TRANSIENT WEAR DISTANCE IN THE ADHESIVE WEAR OF A6061 ALUMINIUM ALLOY REINFORCED WITH ALUMINA PARTICLES

  • Yang, L.J.
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2002.10b
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    • pp.217-218
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    • 2002
  • An integrated adhesive wear model was proposed to determine the transient wear and steady-state wear of aluminium alloy matrix composites. The transient wear volume was described by an exponential equation, while the steady-state wear was governed by a revised Archard equation, in which both the transient wear volume and transient sliding distance were excluded. A mathematical method was developed to determine both the transient distance and the net steady-state wear coefficient. Experimental wear tests were carried out on three types of commercial A6061 aluminum alloy matrix composites reinforced with 10%, 15% and 20% alumina particles. More accurate wear coefficient values were obtained with the proposed model. The average standard wear coefficient, as determined by the original Archard equation, was found to be about 51% higher.

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MERGERS, COSMIC RAYS, AND NONTHERMAL PROCESSES IN CLUSTERS OF GALAXIES

  • SARAZIN CRAIG L.
    • Journal of The Korean Astronomical Society
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    • v.37 no.5
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    • pp.433-438
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    • 2004
  • Clusters of galaxies generally form by the gravitational merger of smaller clusters and groups. Major cluster mergers are the most energetic events in the Universe since the Big Bang. The basic properties of cluster mergers and their effects are discussed. Mergers drive shocks into the intracluster gas, and these shocks heat the intracluster gas. As a result of the impulsive heating and compression associated with mergers, there is a large transient increase in the X-ray luminosities and temperatures of merging clusters. These merger boost can affect X-ray surveys of clusters and their cosmological interpretation. Similar boosts occur in the strong lensing cross-sections and Sunyaev-Zeldovich effect in merging clusters. Merger shock and turbulence associated with mergers should also (re)accelerate nonthermal relativistic particles. As a result of particle acceleration in shocks and turbulent acceleration following mergers, clusters of galaxies should contain very large populations of relativistic electrons and ions. Observations and models for the radio, extreme ultraviolet, hard X-ray, and gamma-ray emission from nonthermal particles accelerated in these shocks will also be described. Gamma-ray observations with GLAST seem particularly promising.

Ferro-nematics and their outlook

  • West, John L.;Jakli, A.;Glushchenko, Anatoliy;Reznikov, Yuri
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.287-288
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    • 2004
  • We report on the development of ferronematic liquid crystals, new materials that consist of a dilute suspension of ferroelectric particles in a nematic liquid crystal host. The particles share their ferroelectric properties with the nematic liquid crystal and impose a spontaneous dielectric polarization of about 10 nC/$cm^2$ to the entire medium, typical for many ferroelectric liquid crystals. As expected, these new materials have enhanced dielectric anisotropy and are sensitive to the sign of an applied electric field. The potential of their use in a number of devices are discussed.

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Characteristics of Formation and Growth of Aerosol Particles in an Indoor Smog Chamber (스모그 챔버 실험에 의한 에어로졸의 생성과 성장 현상의 관찰)

  • 김민철;배귀남;이승복;진현철;문길주;박주연;김용표
    • Proceedings of the Korea Air Pollution Research Association Conference
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    • 2003.05b
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    • pp.43-44
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    • 2003
  • 스모그 챔버는 대기화학 반응을 물리적 변수를 제어할 수 있는 공간 내에서 재현하여 스모그 현상을 체계적으로 규명하기 위해 가장 많이 사용되는 방법이다(Dodge, 2000). 대기화학 반응을 통해 생성된 입자상 물질(secondary particles) 또는 초미세 입자(ultrafine particle, 〈0.1 $\mu\textrm{m}$)는 연속된 물리화학 반응을 통해 accumulation mode(0.1~l.0 $\mu\textrm{m}$) 입자로 성장한다. 특히, 대도시의 시정(visibility)은 accumulation mode 입자의 산란(scattering)과 흡수(adsorption)가 주요한 원인이기 때문에 이러한 물질의 생성에 영향을 주는 가스상 물질의 전화(gas-to-particle conversion) 반응과 초미세 입자의 성장 현상을 규명하는 것은 매우 중요하다. (중략)

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Characteristics of Anion Concentration Distributions in Airbone Fine Particles in Urban Atmosphere (도시대기 중에 부유하는 미세입자중 음이온의 농도분포 특성)

  • 천만영;조기철;여현구
    • Journal of Korean Society for Atmospheric Environment
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    • v.13 no.1
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    • pp.1-7
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    • 1997
  • Anion concentrations and distributions of airborne fine particles less than 2 $\mu$m in urban atmosphere were determined from Feb. 5 to 16, 1996. The sampling was carried out using 8 stages Hering's low pressure impactor (LPI, aerodynamic cutoff diameters are 0.05, 0.075, 0.11, 0.26, 0.50, 1.0, 2.0, and 4.0$\mu$m) on the top of a five-story building located at Kon-Kuk university in Seoul. Average concentrations if C $l^{[-10]}$ , N $O_3$$^{[-10]}$ and S $O_4$$^{2-}$ were 9.4, 8.4, and 14.5 $\mu\textrm{g}$/㎥, respectively. The distributions of these anion concentrations were bimodal types which showed two peaks in the range of 0.075~0.12 ${\mu}{\textrm}{m}$ and 0.5~1 ${\mu}{\textrm}{m}$ in aerodynamic diameter. These results were 2.5~4.7 times higher than anion concentrations collected by Anderson low volume air sampler.

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Effect of Carrier Gas on the Microstructure and Magnetic Properties of Co Nanoparticles Synthesized by Chemical Vapor Condensation (화학기상응축공정(Chemical Vapor Condensation)으로 제조된 Co 나노분말의 미세구조 및 자기적 성질에 미치는 운송기체의 영향)

  • ;X. L. Dong
    • Journal of Powder Materials
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    • v.11 no.1
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    • pp.16-21
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    • 2004
  • The nano-sized Co particles were successfully synthesized by chemical vapor condensation (CVC) process using the precursor of cobalt carbonyl ($Co_2(CO)_8$). The influence of carrier gases on the microstructure and magnetic properties of nanoparticles was investigated by means of XRD, TEM, XPS and VSM. The Co nano-particles with different phases and shapes were synthesized with a change of carrier gas : long string morphologies with coexistence of fcc and hcp structure in Ar carrier gas condition; finer Co core in a mass of cobalt oxide with only fcc structure in He; rod type cobalt oxide phase in Ar+6vol%$O_2$. The saturation magnetization and coercivity was lower in Co nanoparticles synthesized in He carrier gas, due to their finer size.

Biodegradation of Toluene using Biofilms in a Bubble Column Bioreactor

  • Choi, Yong-Bok;Lee, Jang-Young;Kim, Hak-Sung
    • Journal of Microbiology and Biotechnology
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    • v.5 no.1
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    • pp.41-47
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    • 1995
  • Biodegradation of toluene in liquid effluent stream was carried out using biofilms of Pseudomonas putida formed on celite particles in the bubble column bioreactor. Silicon rubber tubing was installed at the bottom of the bioreactor and liquid toluene was circulated within the tubing. Toluene diffused out of the tube wall and was transferred into the culture broth where degradation by biofilms occurred. The operating variables affecting the formation of biofihns on celite particles were investigated in the bubble column bioreactor, and it was found that formation of bifilm is favored by high dilution rate and supply rate of carbon source which stimulate the growth of initially attached cells. Continuous biodegradation of toluene using biofilms was stablely conducted in the bioreactor for more than one month without any significant fluctuation, showing a removal efficiency higher than 95% at the toluene transfer rate of 1.2 g/L/h.

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Proficiency Test for Anions and Metal Ions in Aqueous Solution (수용액중 음이온 및 금속에 대한 숙련도 시험)

  • 이종해
    • Journal of Korean Society for Atmospheric Environment
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    • v.16 no.1
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    • pp.69-77
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    • 2000
  • This paper summerizes the results of the first proficiency testing program for achieving the analytical quality assurance of anions and metal ions in air borne particles in Korea. This program covered the chemical testing of anions(Cl-, NO3-, SO42-) and metals(Fe, Pb, Zn) in qeueous solution. The exercies was conducted by the Measurement & Analysis Committee of Korean Society for Atmospheric Environment(KOSAE). Pilot lab. was Division of Chemical Metrology and Materais Evaluation in Korea Research Institute of Standards and Science(KRISS). A total of 11 laboratories participated in the program. Two samples of 100mL in low density polyehylene bottles were supplied to participating laboratories. The proficiency testing results were interpreted by two graphical methods, Youden polt and x-Charts(control chart).

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