• 한국수정란이식학회지
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• 제25권4호
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• pp.259-262
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• 2010

Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

• 한국미생물·생명공학회지
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• 제20권1호
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• pp.61-67
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• 1992

유기용매 이상계에서의 thermolysin을 사용하여 아스파탐 전구체 합성시 pH, 온도, 기질농도, 그리고 유기용매상에 대한 수용액상의 부비피 ($\alpha$)등의 변화에 따른 기질의 분해 반응, 효소의 안정성, 그리고 Z-APM 합성에 미치는 복합적인 영향을 조사함으로써 반응조건의 최적화를 도모하였다. 유기용매 이상계에서의 L-PM.HCL의 자연분해는 수용액에서보다 훨씬 느리게 일어나며, 또한 $\alpha$가 증가할 수록 분해속도가 감소하는 것을 알 수 있었다.

• 대한환경공학회지
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• 제30권6호
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• pp.666-672
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• 2008

수산물 가공폐수를 AO$_2$공법에 적용하여 유기물, 질소, 인의 동시제거 가능성을 평가 하였다. 처리도 실험은 도시하수처리장에서 가져온 활성슬러지를 담체에 2주 이상 부착시킨 후 반응조 부피 25%로 충진하여 실험을 행하였다. 유입수 농도는 실험기간 동안 COD : 198 - 1,240 mg/L, TN : 75 - 577.4 mg/L, TP : 2.2 - 53.5 mg/L였다. 운전기간동안 평균 제거효율은 COD : 86.5%(65.7 mg/L), TN : 81.4%(53.1 mg/L), TP : 80.6%(4.07 mg/L)로 나타났다. Anaerobic조와 Aerobic조로 구성된 시스템에 유기물 및 영양염류 부하율이 증가되어도 비교적 안정된 형태의 운전이 가능하였다. 사용된 PU-AC 메디아는 높은 MLSS량을 보유하여 AO$_2$ 시스템 내에서 생물학적 처리율을 높일 수 있었던 것으로 사료된다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1995년도 Proceedings of the Korea Automation Control Conference, 10th (KACC); Seoul, Korea; 23-25 Oct. 1995
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• pp.123-126
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• 1995

Characteristics of control system design using Universal Learning Network (U.L.N.) are that a system to be controlled and a controller are both constructed by U.L.N. and that the controller is best tuned through learning. U.L.N has the same generalization ability as N.N.. So the controller constructed by U.L.N. is able to control the system in a favorable way under the condition different from the condition of the control system in learning stage. But stability can not be realized sufficiently. In this paper, we propose a robust control method using U.L.N. and second order derivatives of U.L.N.. The proposed method can realize better performance and robustness than the commonly used Neural Network. Robust control considered here is defined as follows. Even though initial values of node outputs change from those in learning, the control system is able to reduce its influence to other node outputs and can control the system in a preferable way as in the case of no variation. In order to realize such robust control, a new term concerning the variation is added to a usual criterion function. And parameter variables are adjusted so as to minimize the above mentioned criterion function using the second order derivatives of criterion function with respect to the parameters. Finally it is shown that the controller constricted by the proposed method works in an effective way through a simulation study of a nonlinear crane system.

• 한국통신학회논문지
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• 제32권1C호
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• pp.86-93
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• 2007

최근 들어, 국내외에서는 투표용지 대신에 전자적 투표 장치를 이용하는 전자투표 시스템들을 개발하고 있으나 이러한 전자투표 시스템은 전자투표의 초기 단계로서 온라인 투표시스템은 아니다. 따라서 많은 암호학자들은 암호기술을 기반으로 하는 온라인 전자투표에 대한 연구를 하고 있다. 기존의 1-out-of-L 전자투표는 E1Gamal 암호 기법을 기반으로 하고 있다. 본 논문에서는 계산복잡도를 줄이기 위해, r차 잉여암호를 이용하고, E1Gamal 암호 기반의 1-out-of-L 전자투표의 계산복잡도와 제안한 1-out-of-L 전자투표의 계산복잡도를 비교 분석하였으며, 제안한 전자투표 방식이 보다 효율적임을 보여준다. 또한, 새로운 형태의1-out-of-L 전자투표의 투표-취소 기법을 제안한다. 기존의 전자투표 시스템들은 투표 후의 투표-취소 기법에 대해서 간과해 왔다. 본 논문에서는 제안한 투표-취소 기법을 위해, 기존의 r차 잉여암호의 준동형성을 확장한다. 확장된 준동형성은 프라이버시와 전체검증성을 유지하면서 투표를 취소할 수 있다.

• 펄프종이기술
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• 제38권3호
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• pp.13-22
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• 2006

The analogs of cyclic hydroxamic acids containing N-OH group have been proposed to play effective laccase-mediators in kraft pulp bleaching with laccase/mediator system. The existing mediators (NHA, 1-HBT, VA), the best laccase-mediators reported so far, and selected several analogs of cyclic hydroxamic acids were evaluated as a laccase-mediator for kraft pulp bleaching. It was found that NHA was the most effective mediator for the Trychophyton sp. LKY-7 laccase (TrL) in kraft pulp bleaching with TrL/mediator system, increasing about 10% ISO of brightness and decreasing about 2.8 of kappa number after alkaline-peroxide bleaching. Of the cyclic hydroxamic acidstested, the NHP.1(N-hydroxypyridone analog) was shown to enable TrL to effectively degrade lignin in kraft pulp bleaching, demonstrating approximately similar effect with that of NHA. However, the effect of substituent patterns of cyclic hydroxamic acid analogs in kraft pulp bleaching was not observed. The inhibitions of NHA and NHP.1 on TrL were not exhibited in TrL/mediator system. As a new mediator for TrL, NHP.1 was considered to be able to use in kraft pulp bleaching with TrL/mediator system.

• KSBB Journal
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• 제13권5호
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• pp.547-553
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• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.333-337
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• 1994

In order to develop the whey beverage, we examined the optimum conditions for alcohol fermentation by Kluyveromyces tragilis ATCC 46537. The optimum conditions for alcohol production by K. fragilis ATCC 46537 were as follows; pH 4.5, $30^{\cir}C$, with a supplement of 50 g/l of lactose. To develop a continuous production of alcohol from whey, we compared batch fermentation with continuous iermentation in conjunction with UF system. Batch fermentation produced 11.0 g/l of alcohol, whereas pseudocontinuous and continuous fermentation with UF system produced 8.5 g/l of alcohol. To increase the alcohol production, we added 50 g/l of lactose to both fermentations. Batch fermentation with lactose supplement produced 15.7 g/l of alcohol and continuous fermentation with lactose supplement in conjunction with UF system produced 15.0 g/l of alcohol. These results clearly demonstrate that the UF system can be used to increase the alcohol production from whey, supplemented with exogenous lactose.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2002년도 ICCAS
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• pp.88.5-88
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• 2002

A new floating power supply for the current measurement system of a high voltage power line is developed. It is confirmed that, the current measurement system can stably transfer the signal of the power line current about 50 - 300 A by using the proposed power supply. The excellent characteristics are obtained by the steady-state and transient experiments of the proposed circuit. $\textbullet$ The right figure shows the external view of the trial measurement system for 6.6 kV. In order to see the inside, 120 degrees of the insulator is cut. The toroidal coil for the power supply and the Rogowskii coil for the current sensor are both divided into two and fixed on the power line as...$\textbullet$ The proposed circuit can supply +5V and -5V voltages without using a solar cell and/or a battery.

• 대한음성학회:학술대회논문집
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• 대한음성학회 2000년도 7월 학술대회지
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• pp.96-104
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• 2000

The Bodysonic system was invented on the basis of 'Bone Conduction Theory,' which states that people feel sounds with their whole body. The Bodysonic system is used for L2 (English) learning at Aichi Women's Junior College. In recent years we have developed some unique methodology related to use of the Bodysonic system. In Japan it is difficult for adult L2 learners to acquire the prosody of a foreign language. A language laboratory using the Bodysonic system has been suggested as one way to eradicate such adult L2 problems. The Bodysonic system changes sounds into vibrations. It makes it easy for learners to acquire the prosody of a foreign language because humans can convey information, through their tactile organs. In addition, this system was originally designed to make people relax, so it can also help minimize learner anxiety. The effect of Bodysonic vibrations on language learning has already been proven by some experiments. The Bodysonic system appears to be an ideal teaching method for adult to learn a foreign language.