• 한국축산식품학회지
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• 제30권2호
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• pp.236-242
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• 2010

The objective of this study was to investigate the potential of the application of kimchi LAB as starter culture in the production of fermented sausages. For this, the solid-state model media composed to simulate the substantial conditions of meat mixtures were fermented for 120 h after the treatment with different concentrations of kimchi (0.5, 1.0, 1.5, 3.0, and 5.0%) and lyophilized kimchi-powder (0.2 % and 0.5%). During the fermentation period, the growth of total viable cells and LAB, and the changes of pH and titratable acidity were investigated. The initial LAB counts ranged from 7.18 to 8.34 Log CFU/ mL for kimchi media and from 6.93 to 6.94 Log CFU/mL for kimchi-powder media depending on the added concentrations. The kimchi LAB in this study were not influenced by the immobilized condition for their adaptation and growth by showing no lag phase and thus acted similar as in the submerged medium. The initially increased counts reached around 9 Log CFU/ mL in 12 h independent of the concentrations of a ded kimchi. However, the growth and metabolic activity of kimchi-powder LAB were influenced by the immobilized condition. Supposedly, as the nutrient supply in solid-state depended solely on diffusion, these differences in the souring properties were caused by the LAB topography in the medium matrix. Nevertheless, the differences in the numbers of LAB between two media were less than 0.5 Log units and the pH drop in the solidstate batches was quite rapid and reached low values. Therefore, it can be assumed that kimchi and kimchi-powder LAB showed the utility as the substitute of commercial starter culture even without a rehydrating pretreatment.

• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.188-196
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• 2014

Extracellular production of recombinant human bone morphogenetic protein-7 (rhBMP-7) was carried out through the fermentation of Bacillus subtilis. Three significant fermentation conditions and medium components were selected and optimized to enhance the rhBMP-7 production by using the response surface methodology (RSM). The optimum values of the three variables for the maximum extracellular production of rhBMP-7 were found to be 2.93 g/l starch, 5.18 g/l lactose, and a fermentation time of 34.57 h. The statistical optimization model was validated with a few fermentations of B. subtilis in shake flasks under optimized and unoptimized conditions. A 3-L jar fermenter using the shake-flask optimized conditions resulted in a higher production (413 pg/ml of culture medium) of rhBMP-7 than in a shake flask (289.1 pg/ml), which could be attributed to the pH being controlled at 6.0 and constant agitation of 400 rpm with aeration of 1 vvm.

• 한국전산유체공학회지
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• 제21권1호
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• pp.1-9
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• 2016

Atmospheric icing may have significant effects not only on safety of aircraft in air, but also on performance of wind turbine and power networks on ground. Thus, ice protection measure should be developed to protect these systems from icing hazards. A very efficient method is the electro-thermal de-icing based on a process by which ice accretion is melted and blown away through aerodynamic forces. In this computational study, a state-of-the-art icing code, FENSAP-ICE, was used for the analysis of electro thermal de-icing system. Computational results including detailed conjugate heat transfer analysis were then validated with experimental data. Further, the computational model was applied to the DU21 airfoil section of NREL 5MW wind turbine with calculated heater parameters.

• BMB Reports
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• 제33권4호
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• pp.312-316
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• 2000

Effects of various nitrogenous compounds on the peroxidative activity of Korean radish (Rophanus sativus L.) isoperoxidase $A_1$ were examined by using anilino substrates, such as dianisidine and phenylenediamine. We also used phenolic substrates such as guaiacol, chlorogenic acid, caffeic acid, ferulic acid and esculetin. The peroxidation of dianisidine was stimulated by adenine and imidazole as much as 5 fold and 11 fold, respectively at pH 8. Moreover, about 4.8 fold and 8 fold stimulation of phenylenediamine peroxidation occurred by adenine and imidazole, respectively at pH 8. The stimulation by adenine and imidazole did not occur at the acidic pH range. The peroxidations of phenolic substrates, such as guaiacol, chlorogenic acid, caffeic acid, ferulic acid and esculetin, were not boosted greatly by any of the nitrogenous compounds tested. Notably, ammonium salt, which has been known for the excellent booster of horseradish peroxidase, did not affect the peroxidation of the Korean radish isoperoxidase $A_1$. The kinetic studies of dianisidine peroxidation with imidazole, as a model of boosting reaction, showed that neither the affinity of imidazole against dianisidine, nor the activation energy of dianisidine peroxidation changed during the activity boosting of isoperoxidase $A_1$.

• 센서학회지
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• 제3권3호
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• pp.22-27
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• 1994

마이크로 산소분압센서의 특성에 맞는, CMOS op-amp로 구성된 potentiostat 및 I-V converter용 op-amp를 설계하였다. 시뮬레이션 결과, 설계된 potentiostat는 $l.1k{\Omega}$의 낮은 출력저항을 나타냈으며, 산소분압센서의 등가회로와 연결한 경우 $-3{\sim}2.5V$범위의 전압을 선형적으로 인가할 수 있었고 안정한 주파수 특성을 보였다. 또한 I-V converter의 입력 저항은 약 $30{\Omega}$으로 작았으며 좋은 직선성을 나타내었다.

• Korean Chemical Engineering Research
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• 제56권2호
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• pp.252-260
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• 2018

Energy, a critical input, is to be efficiently managed via waste heat recovery and energy reuse for the economic viability of a process industry. In particular, cement manufacture demands a huge quantum of energy, for the necessary reactions. Huge amounts of hot effluent gases are generated. Energy recovery from these waste gases is an area that is of contemporary research interest. Now, about 75% of total heat recovery takes place in the riser of the suspension pre-heater system. This article deals with the hydrodynamic and heat transfer aspects of riser typically used in the cement industry. An experimental apparatus was designed and fabricated with provision for the measurement of gas pressure and solid temperatures at different heights of the riser. The system studied was air - chalcopyrite taken in different particle sizes. Acceleration length ($L_A$) determined at different parametric levels was fitted to an empirical correlation: $L_A/d_t=4.91902(d_p/d_t)^{0.10058}(w_s/w_g)^{-0.11691}(u_g{\mu}_g/d_t^2g{\rho}_g)^{0.28574}({\rho}_p/{\rho}_g)^{0.42484}$. An empirical model was developed for Nusselt number as a function of Reynolds and Prandtl numbers using regression analysis: $Nu=0.40969(Re_p)^{0.99953}(Pr)^{0.03569}$.

• Mycobiology
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• 제44권4호
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• pp.291-301
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• 2016

Culinary mushroom Pleurotus pulmonarius has been popular in Asian countries. In this study, the anti-oxidant, cholinesterase, and inflammation inhibitory activities of methanol extract (ME) of fruiting bodies of P. pulmonarius were evaluted. The 1,1-diphenyl-2-picryl-hydrazy free radical scavenging activity of ME at 2.0 mg/mL was comparable to that of butylated hydroxytoluene, the standard reference. The ME exhibited significantly higher hydroxyl radical scavenging activity than butylated hydroxytoluene. ME showed slightly lower but moderate inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase than galantamine, a standard AChE inhibitor. It also exhibited protective effect against cytotoxicity to PC-12 cells induced by glutamate ($10{\sim}100{\mu}g/mL$), inhibitory effect on nitric oxide (NO) production and inducible nitric oxide synthase protein expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, and carrageenan-induced paw edema in a rat model. High-performance liquid chromatography analysis revealed the ME of P. pulmonarius contained at least 10 phenolic compounds and some of them were identified by the comparison with known standard phenolics. Taken together, our results demonstrate that fruiting bodies of P. pulmonarius possess antioxidant, anti-cholinesterase, and inflammation inhibitory activities.

• Environmental Engineering Research
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• 제19권3호
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• pp.247-253
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• 2014

The aim of this study was to characterize quintinite in fluoride removal from aqueous solutions, using batch experiments. Experimental results showed that the maximum adsorption capacity of fluoride to quintinite was 7.71 mg/g. The adsorption of fluoride to quintinite was not changed at pH 5-9, but decreased considerably in highly acidic (pH < 3) and alkaline (pH > 11) solution conditions. Kinetic model analysis showed that among the three models (pseudo-first-order, pseudo-second-order, and Elovich), the pseudo-second-order model was the most suitable for describing the kinetic data. From the nonlinear regression analysis, the pseudo-second-order parameter values were determined to be $q_e=0.18mg/g$ and $k_2=28.80g/mg/hr$. Equilibrium isotherm model analysis demonstrated that among the three models (Langmuir, Freundlich, and Redlich-Peterson), both the Freundlich and Redlich-Peterson models were suitable for describing the equilibrium data. The model analysis superimposed the Redlich-Peterson model fit on the Freundlich fit. The Freundlich model parameter values were determined from the nonlinear regression to be $K_F=0.20L/g$ and 1/n=0.51. This study demonstrated that quintinite could be used as an adsorbent for the removal of fluoride from aqueous solutions.

• Animal Bioscience
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• 제34권4호
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• pp.471-481
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• 2021

Objective: A comprehensive study was conducted to study the effects of partition of variance on accuracy of genetic parameters and genetic trends of economic traits in Vanaraja male line/project directorate-1 (PD-1) chicken. Methods: Variance component analysis utilizing restricted maximum likelihood animal model was carried out with five generations data to delineate the population status, direct additive, maternal genetic, permanent environmental effects, besides genetic trends and performance of economic traits in PD-1 chickens. Genetic trend was estimated by regression of the estimated average breeding values (BV) on generations. Results: The body weight (BW) and shank length (SL) varied significantly (p≤0.01) among the generations, hatches and sexes. The least squares mean of SL at six weeks, the primary trait was 77.44±0.05 mm. All the production traits, viz., BWs, age at sexual maturity, egg production (EP) and egg weight were significantly influenced by generation. Model four with additive, maternal permanent environmental and residual effects was the best model for juvenile growth traits, except for zero-day BW. The heritability estimates for BW and SL at six weeks (SL6) were 0.20±0.03 and 0.17±0.03, respectively. The BV of SL6 in the population increased linearly from 0.03 to 3.62 mm due to selection. Genetic trend was significant (p≤0.05) for SL6, BW6, and production traits. The average genetic gain of EP40 for each generation was significant (p≤0.05) with an average increase of 0.38 eggs per generation. The average inbreeding coefficient was 0.02 in PD-1 line. Conclusion: The population was in ideal condition with negligible inbreeding and the selection was quite effective with significant genetic gains in each generation for primary trait of selection. The animal model minimized the over-estimation of genetic parameters and improved the accuracy of the BV, thus enabling the breeder to select the suitable breeding strategy for genetic improvement.

• 대한기계학회논문집B
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• 제37권4호
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• pp.323-329
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• 2013

본 연구에서는 나뭇잎 그물맥 구조를 시뮬레이션하기 위해 잎 그물맥을 상 하 보강 박막판과 비압축성 유체가 흐르는 중간층 구조로 모델링하여 다중물리 현상으로 간주하고, 위상 최적화법을 다중물리 설계영역에 적용하였다. Mindlin-Reissner 판 모델에 기인한 횡방향 전단잠금 문제를 해결하기 위해 P1 비적합 요소와 선택 감소 적분법을 이용하였다. 다시-스토크스 유체 유동 채널에 대해 최적설계법을 적용하여 유동 시스템의 다중물리 모델을 해석하였으며, 잎의 그물맥 시뮬레이션을 수행하였다. 계산된 최적형상을 잎의 자연 그물 맥 패턴과 비교하였으며 비슷한 형상을 얻었다. 이와 같은 학제간 연구를 통해 나무 잎 그물맥 시스템을 이해할 수 있는 계기가 될 것으로 사료된다.