• The Korean Journal of Ecology
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• v.22 no.2
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• pp.85-88
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• 1999

Aqueous and dichloromethane (DCM) extracts of leaves, root bark and inflorescences of Parthenium hysterophorus L. at various concentrations were used to quantify its allelopathic potential against Indigofera tinctoria, Amaranthus viridis, A. gangeticus, Phaseolus mungo (cv. CO 1), Sorghum vulgare (cv. SPT- 462). Pennisetum typhoideus (cv. WCC-75) and Eleusine corocana (cv. CO 1). The aqueous extracts were more inhibitory than the DCM extracts to the germination and seedling growth of the plants studied. The relative rate of inhibition increased in the order of inflorescences. leaves and root bark in the aqueous extracts. whereas DCM root bark and inflorescences extracts were promoted at lower concentrations.

• Preventive Nutrition and Food Science
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• v.7 no.2
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• pp.119-122
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• 2002

Oryza sativa cv. Mihyangbyo is one of several recently developed varieties of rice; characterized by high levels of aromatic components, which may increase its sensory and nutritional properties. In conjunction with our continuing investigation of bioactive components of improved grain varieties, a quinolone alkaloid was isolated from the n-butanol soluble fraction of the aleurone layer of Oryza sativa cv. Mihyangbyo (Gramineae) through activity-guided fractionation and isolation. The compound exhibited moderate antineoplastic activity in a human leukemia cell line (U937) with an $IC_{50}$/ value of 118.1 ug/mL, based on the MTT(3-[4, 5]dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) cell proliferation assay. The chemical structure of the functional compound was determined, based on physical and spectroscopic characteristics.

• Journal of Applied Biological Chemistry
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• v.50 no.3
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• pp.127-131
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• 2007

The properties of two cultivars of japonica rice, Odae (early ripening variety) and Ilpum (late ripening variety), were compared. They grew on MS (Murashige and Skoog) medium but the growth of both cultivars was strongly retarded by 50 mM or more salt. There was no clear difference between the growths of seedlings of the two cultivars for the first 24 h after germination. The amylopectin chain-length profiles of the two cultivars did not differ significantly, and amylopectin content was estimated at $16.0{\pm}0.4%$ in cv. Odae and $16.4{\pm}0.4%$ in cv. Ilpum. A total of 114 RAPD (randomly amplified polymorphic DNA) fragments ranging from 0.4 to 2.5 kb were isolated from the two cultivars, 61 from cv. Odae and 53 from cv. Ilpum, indicating that there is little genetic variation between them.

• Applied Biological Chemistry
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• v.47 no.4
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• pp.430-433
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• 2004

Lilium cv. Casablanca pollen grains stored at $-70^{\circ}C$ were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at $27^{\circ}C$. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of $200\;{\mu}M$ acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.

• Korean Journal of Food Science and Technology
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• v.49 no.2
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• pp.209-214
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• 2017

We evaluated the effect of cucumber vinegar (CV) on fatigue accumulation in rats that performed high-intensity exercise. The rats were randomly assigned to 3 groups: sedentary control (SC), exercise control (EC), and CV. Body weights were higher in groups EC and CV than in group SC. Organ weights in group CV did not differ from those in group SC. Running time was significantly longer in group CV than in the other groups. Compared to group EC, cucumber vinegar administration markedly decreased serum concentrations of ammonia, inorganic phosphate, and ${{\small}L}$-lactate. The activities of serum creatine kinase and lactate dehydrogenase were significantly lower in group CV than in groups SC and EC. Glycogen contents in the muscle and liver were higher in group CV than in groups SC and EC. These results suggest that cucumber vinegar can serve as a functional ingredient in the development of a beverage to attenuate fatigue.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.33-36
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• 1998

Guzmania was propagated through in vitro culture of lateral shoots. When new shoots grown in greenhouse were cut and cultured in vitro, contamination rate was very high at about 80% in the first stage of in vitro culture. Among cytokinin treatments for agar medium, 2.0 mg/L BA was most effective for shoot multiplication, and those with 0.5 mg/L kinetin and 0.5~1.0 mg/L BA were favorable for shoot multiplication. BA was more effective for shoot multiplication than kinetin, and shoot multiplication was more enhanced when 2.0 mg/L BA was combined with 0.1~0.5 mg/L IAA than 2.0 mg/L BA alone. The medium with 2.0 mg/L BA and 0.1 mg/L IAA showed the highest rate of shoot multiplication with about 8.7 in shoot number, and those with 2.0 mg/L BA and 0.5~1.0 mg/L IAA also resulted in high multiplication of shoots. Shoots were multiplicated more in liquid rotation culture(80 rpm) with the medium containing 0.5 mg/L BA and 0.1 mg/L IAA than liquid stagnating and solid cultures. Regenerated shoots formed roots very favorably in the medium supplemented with 2.0 mg/L IBA.

• Journal of Acupuncture Research
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• v.18 no.5
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• pp.77-91
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• 2001

Backgrounds and purpose : The acupuncture of oriental medicine is very important in treatments. Until now it was been researched according to the meridian and qi xue(氣血) phenomenon of oriental medicine's theory. Acupuncture will show more objective index to observe the meridian. And then, I studied the effects on the thermal change of the Taeyon($L_9$) and Chungbu($L_1$) following acupuncture stimulation. Objective and Methods : This study was performed from December 1999 to February 2000 on 60 healthy students. The objective was divided into two groups, those are the control group A(N=30) that no acupuncture stimulation, the group B(N=30) of acupuncture stimulation on Taeyon($L_9$). First, in the control group A, I took a picture Taeyon($L_9$) Chungbu($L_1$) Taenung($P_7$) Chondol($CV_{22}$) area for 30 men without any stimulation with the Digital Infrared Thermograph Imaging(D.I.T.l.) and did same area, 10min after. Second, in the acupuncture stimulation group B, we took a picture Taeyon($L_9$) Chungbu($L_1$) Taenung($P_7$) Chondol($CV_{22}$) area for 30 men without any stimulation with the Digital Infrared Th - ermograph Imaging(D.I.T.I.), and then stimulate acupuncture on Taeyon($L_9$) and took a picture same area, 10min after. Results : 1. In healthy men, average skin temperture on Taeyon($L_9$) area was lower than Chungbu($L_1$) area about $3.0^{\circ}C$, in the Lt. Taeyon($L_9$) and Chungbu($L_1$) area was lower than Rt. Taeyon($L_9$) and Chungbu($L_1$) area. 2. In the acupuncture stimulation group B, the skin temperature of both side Taeyon($L_9$) area showed the increase or decrease significantly. But both Taenung($P_7$) area did not showed significantly. 3. In the acupuncture stimulation group B, the skin temperature of both side Chungbu($L_1$) area showed the increase or decrease significantly. But both Chondol($CV_{22}$) area did not showed significantly. 4. The thermal changes of the area which is a meridian point in the Lung Meridian of the acupuncture stimulation group on Taeyon($L_9$) different from other Meridian with significantly change. Conclusion : The acupuncture stimulation on Taeyon($L_9$) affected the thermal change of the area which is a meridian point, in the Lung Meridian. And then I could relate these results with the existence of the meridian and meridian point. Thus, continuous thermographic study will be needed for the existence of the meridian and meridian point.

• Korean Journal of Agricultural Science
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• v.18 no.2
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• pp.157-163
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• 1991

The cultural conditions in shake flasks were investigated under which maximum amounts of laccase produced by a strain of white-rot fungus Coriolus versicolor CV3. The enzyme yields on potato-malt extract medium by the fungus were higher than on other media consisted of onion infusion or malt extract, with maximum activity of $1.50unit/m{\ell}$ culture or 119.5 unit/g mycelium at 11 days of incubation. Maximum yields of laccase and growth were obtained by supplementation of yeast extract or potassium nitrate to the potato-malt extract medium. Addition of 2.5-xylidine at $4{\times}10^{-4}M$ concentration to the medium induced the laccase production 3.1-fold higher than the basal level, while the mycelial growth was somewhat repressed. The pH optimum for the growth and laccase formation by the fungus was between pH 4 to 4.5.

• Journal of Plant Biotechnology
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• v.4 no.3
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• pp.123-127
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• 2002

Herbicide tolerant rice (Oryza sativa L. cv. Ilpumbyeo) cell lines were selected from $\gamma$-ray-irradiated anther-derived cell cultures. The anther-derived cell clusters were small (300 to 400 ${\mu}{\textrm}{m}$ in diameter) and uniform ones that were screened by miracloth filtering. The cell suspensions were very efficient to plate one layer onto agar medium and to screen target cell lines. Herbicide tolerant cell lines were selected by 5 mg/L cyhalofop butyl (CHB) treatment by using the small cell suspensions on agar N6 medium containing 1 mg/L 2,4-D and 0.2 mg/L kinetin. Of the cell lines, one line (CHB-1) showed stable tolerance at 10 mg/L concentration after 6-month culture without herbicide suspension. Growth stability of CHB-1 was similar to that of control cell line on 10 mg/L CHB containing medium. In this experiment we established herbicide tolerant cell line selection system by using anther-derived uniform-cell suspensions with $\gamma$-ray-irradiation.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.64-69
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• 1999

The effect of phytohormones, light and phosphate on in vitro production of ubiquinone 10 from the suspension cultures of Nicotiana tabacum cv. Xanthi callus was investigated. The inoculum size and cultured time in the suspension culture had to be at least over 2 % of medium volume at 15 days for the excellent growth of Xanthi callus. The growth of Xanthi callus in the suspension culture was improved by addition of NAA and 2,4-D, especially NAA 1.0mg/1 alone, at the light condition. The optimal concentration of phytohormone was 0.1 mg/l 2.4-D and 1.0 mg/l NAA for productivity of ubiquinone 10 in the suspenseion of Xanthi callus. Addition of 3mM KH$_2$PO$_4$ to the medium was more effective in promoting ubiquinone-l0 formation than other concentration in the light condition. Content and production of ubiquinone-l0 in the suspension cultures of Xanthi callus were the highest at the MS media containing 0.5mg/L kinetin, 0.5mg/L 2,4-D, 1.0mg/1 NAA, 3mM phosphate and 2 % inoculum in the light.