• Journal of the Korean Chemical Society
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• v.51 no.5
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• pp.412-417
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• 2007

A simple prepared paste electrode (PE) of DNA immobilized on a carbon nanotube was utilized for monitoring the antibacterial agent oxytetracycline (OTC), using square-wave anodic stripping voltammetry (SWASV) and cyclic voltammetry (CV). Given these conditions, SWASV and CV working ranges were observed within 1-10 ngL-1 OTC. In the SWASV and CV for OTC concentrations of 0.1 mgL-1, the relative standard deviations (n=15) were 0.068 and 0.067, respectively. At the optimized condition, the detection limit was found to be 0.4 ngL-1 OTC. This method was applied to the hatchery fish tissue.

• Journal of Environmental Science International
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• v.21 no.11
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• pp.1333-1338
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• 2012

In order to measure the minute amount of Cu(II) in our environment, cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV) were performed for a trace copper assay using bismuth immobilized on a carbon nanotube paste electrode. An analytical working range of 30 to $240{\mu}g/L$ Cu(II) was obtained for CV and SWSV. The SWSV precision obtained was 0.47 % (n = 15) RSD in $30.0{\mu}g/L$ Cu(II). The detection limit obtained was 3.1 ng/L Cu(II) using SWSV, while the CV yielded the nano-range detection limit through the pre-concentration step. By using this research method, Cu(II) value could be determined in the urine of human sample and in the brain of fish sample. This research can be effectively applied to other cases of measuring minute amount of Cu(II) in living organisms.

• Bulletin of the Korean Chemical Society
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• v.28 no.4
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• pp.515-519
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• 2007

Neurotransmitter glycine in the nano gram range was analyzed using a paste electrode (PE) in cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV). An anodic peak caused by oxidation of the glycine ion appeared at the 0.4 V (versus Ag/AgCl/KCl) potential in a 0.1 M NH4H2PO4 electrolyte solution. At optimized conditions, the working range of the SWSV and CV concentration was found to be 5-60 ngL-1 glycine; precision of R2 = 0.9816 (SWSV) and 0.9986 (CV); and detection limit of 0.65 ngL-1 (5.82 × 10-12 molL-1) (S/N = 3). The optimized conditions were applied to an assay in a fish brain tissue and a living brain cell in real time.

• Journal of Plant Biology
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• v.27 no.3
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• pp.173-178
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• 1984

Callus culture of Phaseolus vulgaris L. was carried out to examine the ability to grow on cytokinin-free medium. Of the sixteen cultivars of P. vulgaris, eight were classified as completely cytokinin-autonomous phenotype and five were found to be cytokinin-dependent phenotype. Intermediate phenotype was shown in three cultivars. Using cv. Palgong and ca 21 as cytokinin-dependent genotypes, the genotype responses to the cytokinin requirements of callus tissue were studied in detail. The callus tissue of cv. Palgong and ca 21 were never habituated in cytokinin-free medium, regardless tissue origin and cytokinin concentration in previous passages. The result suggests that cytokinin dependency of callus tissue of P. vulgaris cv. Palgong and ca 21 may be due to inactivation of cytokinin biosynthetic pathway.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.2
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• pp.131-141
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• 2024

In this study, the efficacy of antioxidant and whitening factors was analyzed in order to verify the possibility of use as functional cosmetic materials related to antioxidant and whitening by using the extract of Oryza sativa cv. Heugjinmi. As a result, S OD-like activity, FRAP, reducing power, xanthine oxidase inhibitory activity, and elastase inhibitory activity were similar to those of the control group. Tyrosinase inhibitory activity had no effect in hydrothermal extract and 59% inhibitory activity in ethanol extract. Ethanol extract was found to inhibit cellular tyrosinase inhibitory activity and melanin biosynthesis at concentrations of 25, 50, and 100 ㎍/mL, which will not affect survival in the B16F10 cell line. In addition, the results of confirming the mRNA expression of tyrosinase, TRP-1, and TRP-2 showed inhibition rates of 37%, 51%, and 34%, respectively, at the highest concentration of 100 ㎍/mL. Therefore, it is believed that O. sativa extract has potential to be utilized as a functional cosmetic material related to antioxidant and whitening.

• Journal of Korean Academy of Nursing
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• v.34 no.7
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• pp.1343-1350
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• 2004

Purpose: The purpose of this study was to identify effects of the SP-6 acupressure on dysmenorrhea, the skin temperature of the CV2 acupoint and oral temperatures in the college students. Method: Data was collected from May 1 to August 31, 2002. A total of 58 students from two universities ticipated in the study. Both groups were pretested before the intervention for three variables', the intensity of dysmenorrhea, skin temperature of the CV2 acupoint and oral temperature. Then, SP-6 acupressure was provided for 20 minutes for students in the experimental group. The instruments used in this study included the Visual Analogue Scale developed by Johnson & Rice(1974), Menstrual Attitudes Questionnaire Scale developed by Brooks-Gunn & Ruble(l980), and a Stress scale developed by Cheun and Kim(l991). Result: There were statistically significant differences in the intensity of dysmenorrhea 30minutes after the intervention. The experimental group had a lower intensity than the control group. There were not statistically significant differences in skin temperature of the CV2 acupoint and oral temperature 30minutes after the intervention with the experimental group. Conclusion: SP-6 acupressure reduced the subjective perception of dysmenorrhea.

• Korean Journal of Plant Tissue Culture
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• v.24 no.1
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• pp.53-55
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• 1997

The shoot tips of Delphinium cv. Princess Caroline were cultured on the MS medium supplemented with cytokinin and auxin alone or in combination. Among cytokinins, BA was most effective in shoot multiplication, adequqte concentrations being 1.0-5.0 mg/L. Shoot multiplication was very favorable on the media with 1.0-3.0 mg/L BA and 0.1-0.5 mg/L IAA. Additions of BA and IAA did not stimulate shoot multiplication, but increased a little fresh weight. Shoots were scarcely rooted on the media with IBA or NAA, and were not done utterly on the media containing activated charcoal. Therefore, shoots were treated by Rootone and planted in the cultural media for in vivo rooting. The highest rate of rooting was 68% in the mixed cultural medium composed of Perlite 1 and Vermiculite 1.

• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.209-213
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• 2001

Spathiphyllum floribundum cv. Cupid was micropropagated on LS medium containing various growth regulators. Shoot tips were cultured on media with different concentrations of thidiazuron (TDZ) or banzyladenine (BA). The medium containing 2.0 mg/L BA was most effective on the shoot multiplication and the formation of adventitious multi-shoot cluster from shoot tips among the different concentrations of TDZ and BA. The combined treatments of BA and paclobutrazol (PBZ) were stimulated the shoot multiplication and the formation of adventitious mu]ti-shoot clusters from shoot tips compared to the ones of BA alone. The adventitious multi-shoot clusters were culled with 5∼7 mm in size and cultured on media with BA or thidiazuron (TDZ). Shoot multiplication and shoot cluster proliferation from the shoot clusters were very favorable on media supplemented with 2.0∼3.0 mg/L BA. The shoots grew and rooted out vigorously on media with 2.0∼3.0 mg/L IBA. The rooted plantlets were acclimatized in the soil mixed with a rate of vermiculite 1 and perlite 1, and survived more than 95% in greenhouse.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.506-512
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• 2009

To study the effect of genotype of donor plants on anther culture, anthers of nine hybrid cultivars (Derby, Special, Bossanova, Minipaprika, Fiesta, Boogie, Phenlene, Kufrah, and Clarity) of sweet pepper (Capsicum annuum L.) were cultured in a petridish containing C medium (Sibi, Dumas De Vaulx medium) supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin, 3% sucrose and 0.32% Phytagel. The cultures were incubated in the dark at $35^{\circ}C$ for seven days, and then cultured at $25^{\circ}C$ with a photoperiod of 16 hr. daylength for 40 days. Frequency of callus formation and plant regeneration was varied among cultivars. Callus formation was ranged from 6% in Phenlene to 69.8% in Kufrah. The highest percentage of regenerated plantlets was obtained in cv. Phenlene (2.67%) followed by Bossanova (2.41%). Result of ploidy analysis; chromosome number observation and flowcytometry analysis, showed that haploid plants could be developed from all of these hybrid cultivars except cv. Fiesta, where highest percentage of haploid plants were obtained in Minipaprika (40%) followed by cv. Bossanova (36.1%). Haploid plants derived from these hybrid cultivars contained single set of chromosome (12 in numbers), higher stomata density (numbers), and smaller sized stomata as compare to diploid plants. The mean length of stomata was 26.9 ${\mu}m$ in haploid plants and 35.7 ${\mu}m$ in diploids.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.5
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• pp.1238-1242
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• 2005

Shangwan(CV13), Zhongwan(CV12), Xiawan(CV10), Hefu(L14) and Taichong(LR3) which belong to Conception Vessel and stomach meridian are acupuncture points frequently used for healing gastrointestinal desease in oriental medicine. The present study was conducted to see the effects produced by combined acupuncture(A), electro-acupuncture(EA) and moxibustion using five acupoints which belongs to different meridians on serum gastrin level in rats. Electro-acupuncture (2 Hz, intensity; 10 times muscle twitch threshold) was applied for 30 minutes to Hefu(LI4), Taichong(LR3) under halothane anesthesia. Moxibustion ($1.6{\pm}0.3\;mg$, 5 times of a day) was applied during 3 days to CV10, CV12, CV13 under halothane anesthesia. Combined stimuli of acupuncture at Hefu(LI4), Taichong(LR3) and moxibustion at CV13, CV12 increased serum gastrin level significantly eventough combined simulus of moxibustion on CV 10 was not shown any change. And combined stimul of EA and moxibustion at experimental all groups were not shown any effects. These data suggest that effects of acupuncture and moxibustion are depentent with used acupoints and combination stimuli method.