• Korean Journal of Crystallography
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• v.13 no.1
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• pp.17-20
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• 2002

The complex [Zn(L)₂(H₂O)₄] (1) (L = isonicotinate) has been prepared and characterized by X-ray crystallography. Compound 1 crystallizes in the triclinic space group P1, with a = 6.9062(4) , b = 9.2618(7) , c = 6.3313(3) , α = 104.986(6)°, β = 112.865(4)°, γ = 96.213(6)°, V = 350.41(4) , Z = 1, R₁(wR₂) for 1225 observed reflections of [I > 2σ(I)] was 0.0209 (0.0591). The coordination environment of the zinc atom can be described as an octahedron in which the isonicotinato ligands are mutually trans. Compound 1 is also connected into a two-dimensional chain via hydrogen-bonds.

• Korean Journal of Food Science and Technology
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• v.49 no.1
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• pp.56-62
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• 2017

The purpose of this study was to determine how seeding time changes the properties of soybean products meju and doenjang. Soybeans were seeded on the last day of May (5L), on the mid day of June (6M), and on the last day of June (6L), respectively. The 5L soybeans experienced a distinguishing hot and humid climate at the ripening stage, and these climate conditions resulted in smaller seed sizes. Fermentation briskly progressed in all doenjang until 120 days, and the 5L doenjang exhibited the lowest fermentation efficiency. The 5L soybeans showed the highest GABA, polyphenol contents, and ACE inhibitory activity, and the 6M soybeans showed the highest radical scavenging activity among the groups tested. In doenjang, the functional properties generally increased as the aging time approached the $120^{th}$ day, and the 6M doenjang showed the highest functional properties at the $120^{th}$ day. Therefore, 6M soybeans had the most appropriate characteristics when producing doenjang.

• The Korea Journal of Herbology
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• v.28 no.6
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• pp.129-133
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• 2013

Objectives : The purpose of this study was to investigate the effects of Angelicae acutilobae Radix Water Extract (AA) on the production of cytokines in RAW 264.7 cell stimulated with lipopolysaccharide (LPS). Method : RAW 264.7 cells were cotreated with AA (50 and $100{\mu}g/mL$) and lipopolysaccharide (LPS; $1{\mu}g/mL$) for 24 hours. After 24 hours treatment, using bead-based multiplex cytokine assay, concentrations of various cytokines such as interleukin(IL)-6, tumor necrosis factor-alpha(TNF-${\alpha}$) granulocyte colony-stimulating factor(G-CSF), granulocyte macrophage colony-stimulating factor(GM-CSF), and macrophage inflammatory protein(MIP)-$1{\alpha}$ were measured. Result : AA significantly inhibited LPS-induced production of IL-6 and MIP-$1{\alpha}$ from LPS-stimulated RAW 264.7 cells at the concentration of $50{\mu}g/mL$. AA significantly inhibited LPS-induced production of TNF-${\alpha}$ from LPS-stimulated RAW 264.7 cells at the concentration of $100{\mu}g/mL$. AA significantly inhibited LPS-induced production of G-CSF and GM-CSF in RAW 264.7 cells at the concentrations of 50 and $100{\mu}g/mL$. Conclusion : These results suggest that AA has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as IL-6, TNF-${\alpha}$, G-CSF, GM-CSF, and MIP-$1{\alpha}$ in LPS-induced macrophages.

• KSBB Journal
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• v.13 no.2
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• pp.181-186
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• 1998

In order to obtain high-level production of recombinant human thrombopoietin (rhTPO) in insect cell line, HTI-TN-5B1-4 (TN5), conditions for optimal rhTPO expression such as multiplicity of infection (MOI), the cell density at infection, harvesting time and type of culture method as well as growth media were determined. When TN5 cells were cultured as anchorage-dependent state in 60-mm dish, cell density $2\times^6$ cells,MOI of 10 and Garvesting the culture media at 72 hr post-infection wrere the cinditions for highest rh TPO production. High production of rhTPO was also achieved by using EXPRESS FIVE serum free media rather than SF900II serum free media-1. Anchorage-dependent TN5 cells were adapted as a suspension culture when they were grown in the presence of heparin. TN5 cells were successfully cultured at 0.2 L scale in suspension culture without having aggregation. When TN5 cells were cultured as suspension state, cell density of $0.6\times10^6$ cells/mL, MOI of 1 and harvesting the culture media at 72 hr post-infection, gave the highest yield of rhTPO.

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.6-11
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• 1995

In order to screen microorganism producing lactic acid with high productivity from nature, we used a medium containing 100 g/l glucose and selected several microorganisms producing more than 80 g/l L-lactic acid. We investigated their physiological characteristics and compared them. The best microorganism was identified as Lactobacillus casei subsp. rhamnosus. The optimum pH for growth and production of lactic acid was 6.0 and this strain showed the highest growth rate at around 30$\circ$C , but the optimum temperature for lactic acid production was 45$\circ$C . The growth was inhibited proportionally from 50 g/l to 300 g/l of glucose and the maximal cell mass increased according to increasing the concentration of corn steep liquor (CSL) protein up to 30 g/l. In batch fermentation for lactic acid production, we produced 128 g/l L-lactic acid with 20 g/l CSL protein and 150 g/l glucose in 35 hours. In pH-stat fed-batch fermentation, we were able to produce 183 g/l L-lactic acid.

• Journal of Life Science
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• v.10 no.6
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• pp.598-604
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• 2000

This study was conducted to investigate the effect of simulated acid rain (SAR) on germination, growth, acid buffering capacity and nutrient leaching in Impatiens balsamina L. and Tagetes patula L.. In both species, germination was not inhibited at pH 4.0, but the germination rate decreased at the lower pH of 3.0, showing higher rate in Inpatiens balsamina L. than Tagetes patula L.. As the pH decreases, the growth of radicle was markedly decreased than that of hypocotyl in both species. The plant height, root length, leaf area, total dry weight, relative growth rate and net assimilation rate were inhibited by SAR. The acid buffering capacity in the leaves were increased at pH 4.0, on the other hand, it was shown a tendency to decrease at pH 2.0 in both species. As the pH levels decreased from 5.6 to 2.0, the nutrient leaching from leaves was significantly increased in both species. Based on the results, there are a great difference in the responses to SAR between the two species. In general, Tagetes patula L. represented a higher tolerance to SAR than Impatiens balsamina L.. These results suggested that interspecific variation in the acid buffering capacity and nutrient leaching from leaves may be responsible for the interspecific susceptibility to SAR.

• Proceedings of the KSR Conference
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• 2004.10a
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• pp.1695-1700
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• 2004

Nonpoint pollutants were monitored on railway station land use. Lots of pollutant mass were washed-off in a first hour. The 95$\%$ confidence interval of pollutant concentrations ranges from 61.6 to 115.4 mg/L for TSS(mean= 88.50 mg/L). 103.8$\∼$244.1 mg/L for COD(mean= 174 mg/L) and 7.68$\∼$17.32 mg/L for Oil & Grease(mean= 12.5 mg/L). The various metals were also washed-off during storm runoff from railway station. The ranges of 95$\%$ confidence intervals were 39.2$\∼$84.0 ug/L for total Cu(mean= 61.6 ug/L), 14.0 $\∼$ 25.8 ug/L for total Pb(mean= 19.9 ug/L) and 182.2 $\∼$ 376.1 ug/L for total Zn(mean= 279.2 ug/L). As a result of this research, we concluded that up to 50$\%$ of the total pollutant mass can be removed when first 30$\%$ of the total flow are treating, and it can be a first flush criteria for best management practices.

• Journal of Environmental Health Sciences
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• v.19 no.3
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• pp.22-28
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• 1993

In order to investigate the hygienic chemical conditions of farm waters used as the potable and cleaning water for cow, we examined the pH, turbidity, KMnO$_4$ consumption, total hardness, chlorine, sulfate, NH$_3$-N, NO$_3$-N, lead, maganese, copper, zinc, fluoride and chrome for 78 farm waters around Kyunggi Province. Of 78 farm waters tested, average pH was 6.70+_0.06, turbidity 0.724 $\pm$ 0.081, KMnO$_4$ consumption 4.200 $\pm$ 0.256 mg/l, total hardness 107.46 $\pm$ 6.90 mg/l, NH$_3$-N 0.043 $\pm$ 0.037 mg/l, NO$_3$-N 8.096 $\pm$ 0.652 mg/l, chlorine 21.414 $\pm$ 2.187 mg/l, sulfate 12.737 $\pm$ 1.511 mg/l, lead 0.076 $\pm$ 0.001 mg/l, manganese 0.029 $\pm$ 0.004 mg/l, copper 0.018 $\pm$ 0.002 mg/l, zinc 0.055 $\pm$ 0.005 mg/l, chrome 0.048 $\pm$ 0.002 mg/l and fluorine 0.011 $\pm$ 0.001 mg/l. According to the geological characteristics, the concentrations of total hardness, NO$_3$-N, pH and chlorine in farm waters of Hwasung gun were higher than those in Yangpyung and Kwangju gun. In hygienic chemical items tested, there were high significanc among NO$_3$-N, total hardness, sulfate and chlorine. KMnO$_4$ consumption was significant with NH$_3$-N, sulfate and pH. But in heavy metals, there were significance between lead and copper, copper and chrome, and copper and fluorine.

• Journal of The Korean Society of Agricultural Engineers
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• v.54 no.6
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• pp.29-37
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• 2012

This study was carried out to runoff characteristics in an upland livestock manure compost. Irrigation, runoff and water quality data in the upland were analyzed periodically from May to November in 2011. The observed amount of rainfall, irrigation, runoff for the experimental upland during the investigation period were 1,299.7 mm, 32.0 mm, and 340.7 mm, respectively. The concentrations of T-N in compost and non-compost upland during study period were ranged from 2.09 mg/L to 6.66 mg/L and from 1.99 mg/L to 6.01 mg/L, respectively. which was generally higher than the quality standard of agricultural water (1.0 mg/L). The concentrations of T-P in compost and non-compost upland during study period were ranged from 0.069 mg/L to 0.525 mg/L and from 0.018 mg/L to 0.152 mg/L, respectively. The runoff pollutants loadings of T-N and T-P in compost upland were 10.05 kg/ha and 0.56 kg/ha, respectively. The runoff pollutants loadings of T-N and T-P in non-compost upland were 9.09 kg/ha and 0.26 kg/ha, respectively. The runoff pollutants loadings in T-N and T-P from this study were much lower values than the pollutant loadings of T-N and T-P from the upland published by the others studies. Runoff pollution loadings due to the upland field in order to identify the characteristics of various crops, farming methods and a variety of targets taking into account regional characteristics by conducting continuous monitoring runoff load estimate will be required.

• Journal of the Korea Convergence Society
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• v.6 no.6
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• pp.263-270
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• 2015

L2 learners deal with communication problems encountered during the course of their oral communication by employing what are known as communication strategies(CSs). As CSs play a significant role in communication and language learning, identifying CSs that facilitate communication has become important in L2 research. However, there have been some controversies about how to identify CSs, especially about data sources: conversation data, retrospective interview data, or combining two types of data. Drawing on the advantages of converged data, this study aimed to identify CSs that L2 learners use in their English communication through a convergence analysis of data. In this qualitative case study, the data were collected from Korean L2 learners' conversations and retrospective verbal reports in stimulated recall interviews. Using a data convergence approach, various CSs were identified: definition replacement CSs, message change CSs, time-gaining CSs, and comprehension CSs. This study suggests the effectiveness of CSs in handling L2 communication problems and the usefulness of data convergence in L2 CS research.