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Effect of Zebularine on Chromosomal Association between Meiotic Homoeologous Chromosomes in Wheat Genetic Background (Triticum aestivum L.) (제부라린이 생식세포분열 동안 동조 염색체 사이의 염색체 접합에 미치는 영향)

  • Cho, Seong-Woo;Ishii, Takayoshi;Tsujimoto, Hisashi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.318-325
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    • 2021
  • The objective of this study was to identify the effect of zebularine, a DNA methylation inhibitor, on the chromosomal association between homoeologous chromosomes in the wheat genetic background. Zebularine at a final concentration of 10 µM was used to treat the spikes of the double monosomic wheat addition line (DMA) with one Leymus mollis chromosome and one Leymus racemosus chromosome, both of which were in a homoeologous relationship. In late prophase, zebularine led to chromosome breakage in the Leymus homoeologous chromosomes. Chromosome breakage caused an increase in the frequency of chromosomal associations between the Leymus homoeologous chromosomes. Ordinary DMA showed 65 cells (35.3%) with chromosomal associations and 119 cells (64.7%) with no association, whereas treated DMA showed 102 cells (60.0%) with chromosomal associations and 67 cells (39.4%) with no association. In diakinesis, the Leymus bivalent showed a chromosomal association in the whole euchromatic region. In metaphase, the Leymus bivalent showed association in the whole chromosomal region, unlike other Leymus bivalents with partial chromosomal association. Chromosomal association by chromosome breakage occurred not only between Leymus chromosomes but also between Leymus and wheat chromosomes. The frequency of other chromosomal association (such as fusion and insert) was increased. Chromosome breakage by zebularine treatment is a useful method at the chromosome level as the spores with others are hereditary stable, although the homologous index (h) was not significantly different between ordinary DMA and treated DMA. It is necessary to study how to control zebularine treatment with a more stable concentration for chromosome breakage during meiosis.

Effect of puffing treatment on the quality characteristics of bee pollen and its addition to wheat flour-puff yeot-gangjeong (팽화처리가 화분 및 이를 첨가한 밀엿강정의 품질특성에 미치는 영향)

  • Lee, Jiyea;Surh, Jeonghee
    • Korean Journal of Food Science and Technology
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    • v.54 no.3
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    • pp.306-312
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    • 2022
  • To test bee pollen as an ingredient for heat-processed foods, bee pollen was puffed under heat at various pressures and examined for its physicochemical properties and antioxidant activities. As the puffing intensity increased, the browning index, total reducing capacity, and DPPH radical scavenging activity of the bee pollen increased significantly (p<0.001). This was attributed to the formation of Maillard reaction products during the puffing process. The wheat flour-puff yeot-gangjeong added with puffed bee pollen showed significantly (p<0.01) higher antioxidant activities than its counterpart with raw bee pollen. In addition, the gangjeong retained the physicochemical characteristics of the puffed bee pollen such as color, soluble solids, titratable acidity, and pH. The results showed that the puffed bee pollen could potentially be used as an ingredient in thermally processed foods and retain its superior antioxidant properties.

Effect of the Kneipp Lebensordnung Psychotherapy on Improving Resilience: Preliminary Validation (크나이프 '삶의 질서' 심리요법의 회복탄력성 개선 효과: 예비적인 검증)

  • Hong, Geum Na;Sin, Bang Sik;Song, Kyu Jin;Kim, Hyun Suk;Choi, Min Joo
    • Journal of Naturopathy
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    • v.10 no.2
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    • pp.77-85
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    • 2021
  • Background: It is necessary to verify the resilience of the newly improved Kneipp psychotherapy. Purpose: This study assesses whether Kneipp Lebensordnung psychotherapy; KnLP program improves resilience. Methods: The KnLP program, including salutogenesis, logotherapy, meditation, and resilience training, is reorganized in consideration of Korean culture and sentiments. It was conducted 4 times for 25 adults (once a week, 3 hours a time), and data, KRQ-53 (Korean Resilience Quotient-53) measured intervention, before, and after was compared and analyzed. Results: The data for 9 adults were selected to draw reliable analysis, and it concluded that participants' KRQ-53 mean score increased by 14.66 from 191.56 to 206.22 during and after the program. The score increase by factor in resilience training is 5.89 points for self-regulation skills, 4.89 points for in interpersonal skills, and 3.89 points for positive capacity. Conclusions: KnL program improves participants' resilience skill (p<.05), and especially in self-regulation skill. Subsequent studies with more participants are required to achieve statistically significant and generalized results in the future.

Antioxidant and Antibacterial Activities of Extracts from Different Parts of Sophora japonica L. (회화나무 부위별 추출물의 항산화 및 항균활성)

  • Park, Min Jeong;Kim, Hye Soo;Kim, Han Bi;Lee, Sang Gyun;Cho, Soo Jeong
    • Journal of Life Science
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    • v.32 no.10
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    • pp.792-802
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    • 2022
  • In this study, the antioxidant and antibacterial activities of Sophora japonica extracts were investigated to determine the potential of S. japonica as a functional food and medicinal materials. S. japonica was divided into flowers, fruits, and branches, and ethanol extraction was used. The total polyphenol and flavonoid contents were significantly higher in the flower and fruit extracts than in the branch extracts, but the ABTS and DPPH radical scavenging activity and ORAC value were higher in the branch extracts. Among the ethanol extracts of S. japonica, branch extracts showed strong antibacterial activity against Porphyromonas gingivalis, and the MIC was 0.2 mg/ml. Branch extracts showed bacteriostatic activity against P. gingivalis at a concentration of 0.4 mg/ml or less and bactericidal activity at a concentration of 0.6 mg/ml or more. Biofilm biomass production and cell growth of P. gingivalis in the culture medium treated with the branch extract at a concentration of 0.2-2.0 mg/ml were significantly decreased in a concentration-dependent manner. In addition, the mRNA expression of fimA and mfa1 associated with fimbriae formation in these cultures was suppressed in a concentration-dependent manner. Based on these results, S. japonica branch extracts can be used as functional food and medicinal materials, as demonstrated by their antioxidant and antibacterial activities against P. gingivalis and the inhibition of biofilm formation resulting from P. gingivalis.

Effects of Early Planting and Polyethylene Mulch on Soil for Early Maturing Soybean in Kyeonggi Area (경기지역에서 대두콩에 대한 피복재배효과)

  • Song, Su-Hyeun;Kim, Sung-Ki;Park, Kyeong-Yeol;Kim, Byeong-Hyeon;Ree, Dong-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.1
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    • pp.112-118
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    • 1987
  • This study was carried out to investigate the effects of early planting and polyethylene film (P.E) mulch on early maturing soybean of Korean native cultivar Kyeonggi No. 114 in the Kyeonggi area. It was planted four times at the ten days interval from April I to April 30 in 1985 and 1986. The average soil temperature was 1-5$^{\circ}C$ higher under the polyethylene film mulch condition than the conventional cultural practice condition. Soil moisture content also was higher under P.E mulch condition. Days to emergence was shortened 5-16 days and days to flowering was shortened 3-5 days. The fresh pod yield was increased 12% more at the planting date of April 1 and April 10. Income was increased 70, 83, more at the planting date of April 1 and April 10 under the polyethylene mm mulch conditions.

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Influence of Midsummer Drainage Times on Lodging and Growth of Rice in Direct Drill Seeding Culture on Puddled Soil (벼 무논골뿌림 재배시 중간낙수 회수가 도복 및 생육에 미치는 영향)

  • Kim, Sang-Su;Choi, Weon-Young;Seok, Soon-Jong;Lee, Seon-Yong;Kim, Jong-Ho;Cho, Dong-Sam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.33-38
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    • 1995
  • This experiment was conducted to elucidate the effect of the midsummer drainage times on lodging characters, field lodging and yield in direct seeding rice culture in puddled soil. Dongjinbyeo was seeded at May 11 by seeding machine with 4cm of furrow depth and drained, one(50days after seeding DAS)) two (30DAS and 50DAS), and three times (30DAS, and 50DAS and 60DAS). The results are as follows. Culm length was shorten and the wall of N3, N4 were thicken as the drain-age times were increased. As the drainage times were increased breaking weights were increased but the lodging index were decreased. Lodging was occurred seriousely at none drainage, but wasn't occurred two or three times drainage. Yield was higher at 1~2 times drainage compared with none and three times drainage. Therefore, two times midsummer drainage is recommend-able water management method for direct seeding rice culture in puddled soil.

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A Case of Psychogenic Tremor Improved by Complex Korean Medicine Treatment Including Cheongshimondam-tang-gamibang and Acupuncture Treatment - A Case Report (청심온담탕가미방과 침구치료를 포함한 복합한의치료로 호전된 심인성 떨림 환자 1례 - 증례보고)

  • Jihyun Lee;Seokyeong Yoon;Hyoenjun Cheon;Sungjun Joo;Jisu Lee;Jungtae Leem;Yanghee Han
    • The Journal of Internal Korean Medicine
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    • v.43 no.6
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    • pp.1186-1197
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    • 2022
  • Objectives: A case study about the effectiveness of integrative Korean medicine treatment in a psychogenic tremor patient. Case presentation: A 36-year-old male diagnosed with psychogenic tremor with low back pain underwent 29 days of inpatient treatment and 4 weeks of outpatient treatment. His pattern identification was a psychogenic tremor of the weakness of the heart and gall bladder (SimDamHeoGup) type. The treatments were herbal medicine and acupuncture. He took Cheongshimondam-tang for 29 days and Chunwangbosim-dan for 6 weeks when he had symptoms. He also received acupuncture for 20 minutes twice a day at GB20 (Pungji), L14 (Hapgok), HT4 (Yeongdo), PC6 (Naegwan), HT7 (Sinmun), GB34 (Yangleungcheon), ST36 (Joksamni), SP6 (Sameumgyo), and LR3 (Taechung). After 8 weeks of treatment, the Fahn Tolosa Marin rating (FTM) scale of his tremor, at rest, dropped from Grade 4 on both upper extremities, trunk, and both lower extremities at the time of admission to Grade 2 in both upper extremities and trunk, and Grade 0 in both lower extremities at the time of discharge. His Beck Anxiety Inventory (BAI) score decreased from 38 to 7, and his numerical rating scale (NRS) decreased from 8 to 1 for low back pain and from 9 to 2 for tremor. A follow-up visit to the hospital 2 months after the end of treatment confirmed continued symptom improvements and no significant side effects. Conclusions: This study suggests the possibility of treating psychogenic tremor using only Korean medicine treatments. Further studies with control groups and long-term follow-up are needed.

The Vascular Plants of Forested Wetland in Daegu-Gyeongbuk, Korea - Focused on Daegu, Cheongdo, Gyeongju and Pohang - (대구, 경북지역 산림습원 일대의 관속식물상 - 대구, 청도, 경주, 포항을 중심으로 -)

  • Myung Hoon Yi;Sung Tae Yoo;Byung Do Kim;Jung Won Yoon;Jong Bin An;Hye Yeon Kwon
    • Korean Journal of Plant Resources
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    • v.36 no.1
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    • pp.32-61
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    • 2023
  • To investigate the distribution of vascular plants growing at Wetland in Daegu·Gyeongbook, we surveyed from June 2020 to October in 2021. The flora of wetland was classified as a total of 459 taxa comprising of 104 families, 286 genus, 406 species, 12 subspecies, 34 varieties, 6 forma, 1 hybrid. Among them, the rare plants species 5 taxa, endemic plants 11 taxa, floristic regional indicator plants 57 taxa, naturalized plants 27 taxa and wetland plants 74 taxa were recorded. The percentage of urbanization index was 8.44%, the naturalized ratio was 5.88%, respectively.

Growth and Grain Yield under Different Direct Seeding Cultures in Rice (벼 직파재배 유형에 따른 생육 및 수량)

  • Choi, Weon-Young;Kim, Sang-Su;Sin, Hyun-Tak;Cho, Soo-Yeon;Choi, Sun-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.1
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    • pp.14-21
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    • 1997
  • This experiment was conducted to identify the growth and yield differences in rice cultural types: Broadcasting on flooded paddy surface(BF), drilling on flooded paddy surface(DF), puddled-soil drill seeding(PD), drill seeding on dried paddy flat(DD), and machine transplanting of lO-day old seedling(MT) at National Honam Agricultural Experiment Station in 1995. Among the cultural types of direct seeding, the number of seedling stand showed high in order of DD>BF>DF>PD. Tillering at early growth stage was faster in MT and effective tiller showed higher in MT than in direct seeding. Growth duration from seeding to heading was longer in direct seeding than in MT for 2-13 days of Dongjinbyeo and 10-18 days of Nonganbyeo. Root distribution ratio at the surface soil revealed high in order of BF>DF>PD>DD and MT. Culm wall thickness of 4th internode was thicker in machine transplanting of 10-day old seedling than in direct seeding, and the depth of buried culm showed deep by turns of MT> DD>PD>DF and BF. The height of center gravity and moment related to lodging revealed higher in direct seeding than machine transplanting that resulted high lodging index by turns of BF>DF>DD> PD among the direct seeding cultural type. Lodging was occured seriously in the order of BF>DF>PD>DD =MT in Dongjinbyeo but wasn't significantly different in Nonganbyeo among the cultural type. The yield components and grain yield showed varietal differences but was not significantly different between in MT and in direct seeding, and among cultural types of direct seeding.

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Strength Anisotropy through Artificial Weak Plane of Mudstone (인공연약면을 따른 이암의 강도이방성에 관한 연구)

  • Lee, Young-Huy;Jeong, Ghang-Bok
    • Journal of the Korean Geotechnical Society
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    • v.24 no.11
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    • pp.111-120
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    • 2008
  • The characteristic of induced anisotropy is investigated in this study for the Pohang mudstone involving the cut plane discontinuity. The uniaxial and triaxial compression tests are performed for anisotropic rocks with artificial joint to look into anisotropic strength characteristics. Both the uniaxial compressive strength and triaxial compressive strength show the lowest value at the angle of cut plane, ${\beta}=30^{\circ}$ and the shoulder type of anisotropy is obtained. Anisotropy ratio (Rc) in uniaxial compression measures 9.0, whereas Rc=1.29-1.98 in triaxial compression is appeared. A series of analyses are made with the test results to derive the suitable parameter values when it is applied to the Ramamurthy (1985) failure criterion. The result of uniaxial compression test is analyzed by introducing the n-index into Ramamurthy failure criterion. The result shows that, n=l is suitable for ${\beta}=0^{\circ}{\sim}30^{\circ}$ and n=3 is suitable for ${\beta}=30^{\circ}{\sim}90^{\circ}$. To analyze the result of triaxial compression test by Ramamurthy failure criterion, anisotropy ratio in uniaxial compression test is added to Ramamurthy's equation and material constants are estimated by modified Ramamurthy's equation. When these values are applied back to Ramamurthy failure criterion, the predicted values are well fitted to the test results. And strength anisotropy for failure criteria of Jaeger (1960), McLamore & Gray (1967) and Hoek & Brown (1980) are also investigated.