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THE USE OF NEAR INFRARED REFLECTANCE SPECTROSCOPY(NIRS) TO PREDICT CHEMICAL COMPOSITION ON MAIZE SILAGE

  • D.Cozzolino;Fassio, A.;Mieres, J.;Y.Acosta
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1610-1610
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    • 2001
  • Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.

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Assessment of the Completeness of Lymph Node Dissection Using Near-infrared Imaging with Indocyanine Green in Laparoscopic Gastrectomy for Gastric Cancer

  • Kim, Tae-Han;Kong, Seong-Ho;Park, Ji-Ho;Son, Yong-Gil;Huh, Yeon-Ju;Suh, Yun-Suhk;Lee, Hyuk-Joon;Yang, Han-Kwang
    • Journal of Gastric Cancer
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    • v.18 no.2
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    • pp.161-171
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    • 2018
  • Purpose: This study assessed the feasibility of near-infrared (NIR) imaging with indocyanine green (ICG) in investigating the completeness of laparoscopic lymph node (LN) dissection for gastric cancer. Materials and Methods: Patients scheduled for laparoscopic gastrectomy for treating gastric cancer were enrolled in the study. After intraoperative submucosal ICG injection (0.05 mg/mL), LN dissection was performed under conventional laparoscopic light. After dissection, the LN stations of interest were examined under the NIR mode to locate any extra ICG-stained (E) tissues, which were excised and sent for pathologic confirmation. This technique was tested in 2 steps: infra-pyloric LN dissection (step 1) and review of all stations after proper radical node dissection (step 2). Results: In step 1, 15 patients who underwent laparoscopic pylorus-preserving gastrectomy (LPPG) and 15 patients who underwent laparoscopic distal gastrectomy (LDG) were examined. Seven and 2 E-tissues were obtained during LPPG and LDG, respectively. From the retrieved E-tissues, 1 and 0 tissue obtained during LPPG and LDG, respectively, was confirmed as LN. In step 2, 20 patients were enrolled (13 D1+ dissection and 7 D2 dissection). Six E-tissues were retrieved from 5 patients, and 1 tissue was confirmed as LN in the pathologic review. Overall, 15 E-tissues were detected and removed, and 2 tissues were confirmed as LNs in the pathologic review. Both nodes were from LN station #6, with 1 case each in the LDG and LPPG groups. Conclusions: NIR imaging may provide additional node detection during laparoscopic LN dissection for gastric cancer, especially in the infra-pyloric area.

Oceanic Application of Satellite Synthetic Aperture Radar - Focused on Sea Surface Wind Retrieval - (인공위성 합성개구레이더 영상 자료의 해양 활용 - 해상풍 산출을 중심으로 -)

  • Jang, Jae-Cheol;Park, Kyung-Ae
    • Journal of the Korean earth science society
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    • v.40 no.5
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    • pp.447-463
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    • 2019
  • Sea surface wind is a fundamental element for understanding the oceanic phenomena and for analyzing changes of the Earth environment caused by global warming. Global research institutes have developed and operated scatterometers to accurately and continuously observe the sea surface wind, with the accuracy of approximately ${\pm}20^{\circ}$ for wind direction and ${\pm}2m\;s^{-1}$ for wind speed. Given that the spatial resolution of the scatterometer is 12.5-25.0 km, the applicability of the data to the coastal area is limited due to complicated coastal lines and many islands around the Korean Peninsula. In contrast, Synthetic Aperture Radar (SAR), one of microwave sensors, is an all-weather instrument, which enables us to retrieve sea surface wind with high resolution (<1 km) and compensate the sparse resolution of the scatterometer. In this study, we investigated the Geophysical Model Functions (GMF), which are the algorithms for retrieval of sea surface wind speed from the SAR data depending on each band such as C-, L-, or X-band radar. We reviewed in the simulation of the backscattering coefficients for relative wind direction, incidence angle, and wind speed by applying LMOD, CMOD, and XMOD model functions, and analyzed the characteristics of each GMF. We investigated previous studies about the validation of wind speed from the SAR data using these GMFs. The accuracy of sea surface wind from SAR data changed with respect to observation mode, GMF type, reference data for validation, preprocessing method, and the method for calculation of relative wind direction. It is expected that this study contributes to the potential users of SAR images who retrieve wind speeds from SAR data at the coastal region around the Korean Peninsula.

Magnetic Properties of Electroless Co-Mn-P Alloy Deposits (무전해 Co-Mn-P 합금 도금층의 자기적 특성)

  • Yun, Seong-Ryeol;Han, Seung-Hui;Kim, Chang-Uk
    • Korean Journal of Materials Research
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    • v.9 no.3
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    • pp.274-281
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    • 1999
  • Usually sputtering and electroless plating methods were used for manufacturing metal-alloy thin film magnetic memory devices. Since electroless plating method has many merits in mass production and product variety com­pared to sputtering method, many researches about electroless plating have been performed in the United State of America and Japan. However, electroless plating method has not been studied frequently in Korea. In these respects the purpose of this research is manufacturing Co-Mn-P alloy thin film on the corning glass 2948 by electroless plating method using sodium hypophosphite as a reductant, and analyzing deposition rate, alloy composition, microstructure, and magnetic characteristics at various pH's and temperatures. For Co-P alloy thin film, the reductive deposition reaction 0$\alpha$urred only in basic condition, not in acidic condition. The deposition rate increased as the pH and temperature increased, and the optimum condition was found at the pH of 10 and the temperature of $80^{\circ}C$. Also magnetic charac­teristics was found to be most excellent at the pH of 9 and the temperature of $70^{\circ}C$, resulting in the coercive force of 8700e and the squareness of 0.78. At this condition, the contents of P was 2.54% and the thickness of the film was $0.216\mu\textrm{m}$. For crystal orientation, we could not observe fcc for $\beta$-Co. On the other hand,(1010), (0002), (1011) orientation of hcp for a-Co was observed. We could confirm the formation of longitudinal magnetization from dominant (1010) and (1011) orientation of Co-P alloy. For Co-Mn-P alloy deposition, coercive force was about 1000e more than that of Co P alloy, but squareness had no difference. For crystal orientation, (l01O) and (lOll) orientation of $\alpha$-Co was dominant as same as that of Co- P alloy. Likewise we could confirm the formation of longitudinal magnetization.

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A Study on the Vibration Characteristics of Critical Speed for Rotor Shaft (회전샤프트의 위험속도에 관한 진동특성 연구)

  • Son, Choong-Yul;Lee, Kang-Su;Ryu, Young-Hyun
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.18 no.9
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    • pp.961-971
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    • 2008
  • In the design of a rotor shaft, care should be taken to minimize vibration by taking into account the sources of vibration. In addition, the intensity critical speed, stability, and other related aspects of the system must be considered. especially when it is operated at a critical speed, it is important to address issues related to vibration, as an increase in the whirling response of the rotor shaft can cause damage to the shaft, destruction of the rotor parts, and detrimental abrasions on the bearings. In this thesis, the vibration characteristics of a rotor shaft are investigated through the use of the finite element method. Variations of the diameters and lengths were used to determine the effect of a rotor shaft using Beam No.188(3D linear strain beam) in ANSYS version 11.0 as a universal interpretation program for finite elements. Special care was taken to prevent excessive vibration, which can result from resonance at the initial stage, in the formulation of a dynamic design for a rotor shaft through calculations while changing the diameters and the lengths of the shaft. Moreover, the dynamic characteristics of the critical speed, total mass, D/L(diameter to length) ratio, and natural frequency were verified. Furthermore, the rotor shaft applied by bearing element was calculated and compared by using Combi No. 214(2-D spring-damper bearing).

An Experimental Study on a Rectangular Parallelepiped Sodium Heat Pipe for High Temperature Class Forming (고온 유리 성형 공정을 위한 직육면체형 Sodium 히트파이프의 실험 연구)

  • Park, Soo-Yong;Boo, Jun-Hong;Kim, Jun-Beom
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.11
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    • pp.1622-1629
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    • 2002
  • To enhance isothermal characteristics of glass-farming surface, a rectangular parallelepiped heat pipes was fabricated, tested, and analyzed. The working fluid was sodium and the wall material was stainless steel 304. The dimension of the heat pipe was 210 (L) $\times$ 140(W) $\times$ 92(H)mm. A lattice structure covered with screen mesh was inserted to promote return of working fluid. The bottom side of heat pipe was heated electrically and the top side was cooled by liquid circulation. The temperature distribution at the bottom surface was of major concern and was monitored to determine isothermal characteristics. A frozen start-up of rectangular parallelepiped liquid metal heat pipe was tested. The operating mode of the sodium heat pipe was affected by the temperature of cooling zone, input heat flux, and the operating temperature of heat pipe. The heat pipe operated in a normal fashion as long as the heat flux was over 5.78W/cm$^2$, and the inside wall temperature of condenser part was above 95$^{\circ}C$ The maximum temperature difference at the bottom surface was observed to be 32$^{\circ}C$ when the operating temperature of the heat pipe was operating normally around 50$0^{\circ}C$. The result showed that a sodium heat pipe was very effective in reducing significantly the temperature difference in the glass-forming surface.

Caffeine Induces High Expression of cyp-35A Family Genes and Inhibits the Early Larval Development in Caenorhabditis elegans

  • Min, Hyemin;Kawasaki, Ichiro;Gong, Joomi;Shim, Yhong-Hee
    • Molecules and Cells
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    • v.38 no.3
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    • pp.236-242
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    • 2015
  • Intake of caffeine during pregnancy can cause retardation of fetal development. Although the significant influence of caffeine on animal development is widely recognized, much remains unknown about its mode of action because of its pleiotropic effects on living organisms. In the present study, by using Caenorhabditis elegans as a model organism, the effects of caffeine on development were examined. Brood size, embryonic lethality, and percent larval development were investigated, and caffeine was found to inhibit the development of C. elegans at most of the stages in a dosage-dependent fashion. Upon treatment with 30 mM caffeine, the majority ($86.1{\pm}3.4%$) of the L1 larvae were irreversibly arrested without further development. In contrast, many of the late-stage larvae survived and grew to adults when exposed to the same 30 mM caffeine. These results suggest that early-stage larvae are more susceptible to caffeine than later-stage larvae. To understand the metabolic responses to caffeine treatment, the levels of expression of cytochrome P450 (cyp) genes were examined with or without caffeine treatment using comparative microarray, and it was found that the expression of 24 cyp genes was increased by more than 2-fold (p < 0.05). Among them, induction of the cyp-35A gene family was the most prominent. Interestingly, depletion of the cyp-35A family genes one-by-one or in combination through RNA interference resulted in partial rescue from early larval developmental arrest caused by caffeine treatment, suggesting that the high-level induction of cyp-35A family genes can be fatal to the development of early-stage larvae.

Development and Performance Test of a l00hp HTS Motor

  • Sohn, M.H.;Baik, S.K.;Lee, E.Y.;Kwon, Y.K.;Yun, M.S.;Moon, T.S.;Park, H.J.;Kim, Y.C.;Ryu, K.S.
    • Progress in Superconductivity and Cryogenics
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    • v.6 no.4
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    • pp.27-31
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    • 2004
  • This paper describes the development and fabrication of a high temperature superconducting motor which consists of HTS rotor and air-core stator. The machine was designed for the rated power of 100hp at 1800 rpm. The HTS field windings are composed of the double-pancake coils wound with AMSC's SUS-reinforced Bi-2223 tape conductor. These were assembled on the support structure and fixed by a bandage of glass-fiber composite. The cooling system is based on the heat transfer mechanism of the thermosyphon by using GM cryocooler as cooling source. The cold head is in contact with the condenser of a Ne-filled thermosyphon. The rotor assembly was tested independently at the stationary state and combined with stator. Characteristic parameters such as reactances, inductances, and time constants were determined to obtain a consistent overview of the machine operation properties. This motor has met all design parameters by demonstrating HTS field winding, cryogenic refrigeration systems and an air-core armature winding cooled with air. The HTS field winding could be cooled down below 30K. No-load test of open-circuit characteristics(OCC) and short-circuit characteristics(SCC) and load test with resistive load bank were conducted in generator mode. Maximum operating current of field winding at 30K was 120A. From OCC and SCC test results synchronous inductance and synchronous reactance were 2.4mH, 0.49pu, respectively. Efficiency of this HTS machine was 93.3% in full load(100hp) test. This paper will present design, construction, and basic experimental test results of the 100hp HTS machine.

HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties (콩 함유 사포닌의 종류 및 함량 분석)

  • Han, Sang-Jun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.244-249
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    • 2011
  • A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC/MS/MS) assay was developed for the determination of soyasaponins in soybean. Among soyasaponins, soyasaponin I was isolated and characterized from methanol extracts of soybean as analytical standards and the development of a new analytical procedure for quantification of its content in various cultivars. The structures of these compound was elucidated by $^1H$, $^{13}C$ NMR experiments and by mass spectrometric analysis. Aqueous ethanol extracts of soybean samples were injected on an Agilent XDB-C18 column ($4.6mm{\times}50mm$, $1.8{\mu}m$) with a mobile phase consisting of 10 mM ammonium acetate-acetonitrile, a flow rate of 0.3 mL/min and a total run time of 8 min. Detection was performed by mass spectrometer bin the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI) m/z at 941 ${\rightarrow}$ 615 for soyasaponin I. In the 9 soybean samples, contents of soyasaponin I ranged from 205 to 726 mg/kg, and correlated negatively with seed size.

SHEAR BOND STRENGTH OF SELF-ETCHING ADHESIVES TO DENTIN AND SEM ANALYSIS (상아질에 대한 자가 산부식 접착제의 전단결합강도와 SEM 분석 비교)

  • Cho, Young-Gon;Roh, Kee-Sun;Kim, Soo-Mee;Lee, Young-Gon;Jeong, Jin-Ho;Ki, Young-Jae
    • Restorative Dentistry and Endodontics
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    • v.28 no.3
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    • pp.222-231
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    • 2003
  • The purpose of this study was to compare shear bond strength and interfacial pattern of composite bond-ed to dentin using self-etching adhesive systems. Sixty extracted human molars with exposed occlusal dentin were divided into four groups and bonded with four adhesives and composites. Single Bond/Filtek Z 350(SB), Tyrian SPE-One-Step Plus/Aelitefil(TY), Prompt L-Pop/Filtek Z 250(LP), and One-Up Bond F/palfique Toughwell(OU). The results of this study were as follows; 1 Shear bond strength for OU was significantly lower than that of other groups(p<0.05). No significant difference was founded among SB, TY, and LP. 2. Failure modes to dentin showed adhesive and mixed for SB TY and LP, but them for OU showed adhesive in all spceimens. 3. Dentin-resin interface showed close adaptation for SB, TY, and LP, but it showed gap for OU. 4. The hybrid layers for TY, LP OU were thinner than that of SB. Adhesive layers were observed between composite and hybrid layer, which were 5 $\mu\textrm{m}$ thick for TY and 10 $\mu\textrm{m}$ thick for OU.