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Combined effect of initial biomass density and nitrogen concentration on growth and astaxanthin production of Haematococcus pluvialis (Chlorophyta) in outdoor cultivation

  • Wang, Junfeng;Sommerfeld, Milton R.;Lu, Congming;Hu, Qiang
    • ALGAE
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    • v.28 no.2
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    • pp.193-202
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    • 2013
  • Nitrogen availability and cell density each affects growth and cellular astaxanthin content of Haematococcus pluvialis, but possible combined effects of these two factors on the content and productivity of astaxanthin, especially under outdoor culture conditions, is less understood. In this study, the effects of the initial biomass densities IBDs of 0.1, 0.5, 0.8, 1.5, 2.7, 3.5, and 5.0 g $L^{-1}$ DW and initial nitrogen concentrations of 0, 4.4, 8.8, and 17.6 mM nitrate on growth and cellular astaxanthin content of H. pluvialis Flotow K-0084 were investigated in outdoor glass column photobioreactors in a batch culture mode. A low IBD of 0.1 g $L^{-1}$ DW led to photo-bleaching of the culture within 1-2 days. When the IBD was 0.5 g $L^{-1}$ and above, the rate at which the increase in biomass density and the astaxanthin content on a per cell basis was higher at lower IBD. When the IBD was optimal (i.e., 0.8 g $L^{-1}$), the maximum astaxanthin content of 3.8% of DW was obtained in the absence of nitrogen, whereas the maximum astaxanthin productivity of 16.0 mg $L^{-1}\;d^{-1}$ was obtained in the same IBD culture containing 4.4 mM nitrogen. The strategies for achieving maximum Haematococcus biomass productivity and for maximum cellular astaxanthin content are discussed.

A Study on Operating Condition of Test-Bed Plant using Membrane filtration of D Water Treatment Plant in Gwang-Ju (D정수장 정밀여과막 실증플랜트의 최적 운전조건 연구)

  • Yang, Hyung-Jae;Yi, Seung-Hoon;Moon, Kyung-Ran
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.3
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    • pp.155-163
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    • 2017
  • Membrane filtration has become more popular in drinking water treatment recently, since the filtration can control not only particulate matters but also pathogenic microorganisms such as giardia and cryptosporidium very effectively. Pilot-scale ($120m^3/d$ of treatment capacity) and test-bed ($25,000m^3/d$ of treatment capacity) microfiltration experiments were conducted to find optimum operating mode and the critical flux. Optimum operating mode of pilot-test was assessed as inflow 1.0 min, filtration 36.5 min, air backwash 0.9 min, backwash 1.0 min and outflow 1.0 min with 50 LMH ($L/min{\cdot}m3^$) of critical flux. Critical Flux was calculated to be $50L/m^2-h$ (within TMP 0.5 bar) based on the increase formula of the transmembrane pressure difference according to the change of time at Flux 20, 40, 56 and 62 LMH in pilot operation. Chemical cleaning was first acid washed twice, and alkali washing was performed secondarily, and a recovery rate of 95% was obtained in the test-bed plant. The results of operating under these appropriate conditions are as follows. Turbidity of treated water were 0.028, 0.024, 0.026 and 0.028 NTU in spring, summer, autumn and winter time, respectively. Microfiltration has superior treatment capability and performance characteristics in removing suspended solids and colloidal materials, which are the main cause of turbidity and important carrier of metal elements, and it has shown great potential in being an economically substitute to traditional processes (sand filtration).

User's Perception Characteristics on Color and Contrast for Designing the Interface of a Combined PC and TV Monitor (PC&TV 겸용모니터의 인터페이스 디자인을 위한 사용자의 색채 및 대비 지각 특성)

  • Jeong, Gwang-Tae;Park, Jae-Hui
    • Journal of the Ergonomics Society of Korea
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    • v.17 no.2
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    • pp.25-32
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    • 1998
  • A combined PC and TV monitor has been developed in several companies. The characteristics of physical user interface in this monitor are likely to vary from existing monitors. Therefore, two experiments to investigate the physical interface characteristics of this monitor were conducted. The first experiment was to investigate the characteristics of color perception with monitor coating (coating vs. non-coating) and screen brightness (30fL vs. 35fL) in the PC mode. The second experiment was to investigate the characteristics of luminance contrast with monitor coating and image movement (static vs. dynamic) in the TV mode. Twenty-three subjects (male 12, female 11) were participated in this experiment. In the first experiment, average color temperatures were obtained in all experimental conditions. There was a significant difference between coating and non-coating screen at 0.1 level. In the second experiment, average luminance contrasts were obtained in all experimental conditions. There was a significant difference between coating and non-coating screen at 0.05 level. In addition, there was a significant difference between static picture and dynamic picture at 0.1 level.

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Determination of Ultratraces of Rhodium by Adsorptive Stripping Voltammetry of Formaldehyde Complex

  • Hong Tae-Kee;Czae Myung-Zoon;Lee Chul;Kwon Young-Soon;Hong Mi-Jeong
    • Bulletin of the Korean Chemical Society
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    • v.15 no.12
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    • pp.1035-1037
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    • 1994
  • An ultrasensitive and selective stripping voltammetric scheme for the determination of rhodium is described. By the use of combined accumulation and catalytic effects in formaldehyde-hydrochloric acid medium, substantial improvement in the limit of detection can be obtained. Optimal experimental conditions were found to be 0.42 M hydrochloric acid solution containing 0.008${\%}$ formaldehyde, an accumulation potential of -0.70 V (vs. Ag/AgCl) and an accumulation time of 20 s. The stripping mode was differential pulse voltammetry. In these conditions the limit of detection lies at 2 ${\times}$ l0$^{-12}$ M (0.21 ppt). The relative standard deviation at 5 ${\times}$ l0$^{-11}$ M was 4.9${\%}$ (n=5). There were no serious interferences from other platinum group metal ions being the tolerable amounts more than 500 times that of rhodium.

Fish length dependance of acoustic target strength for large yellow croaker (부세에 대한 음향반사강도의 체장 의존성)

  • 강희영;이대재
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.39 no.3
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    • pp.239-248
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    • 2003
  • This paper was conducted as an attempt in order to construct the data bank of target strength for acoustic estimation of fish length in the coastal waters of Korea. The fish length dependence of acoustic target strength for 13 large yellow croakers (Pseudosciaena crocea) at 75 kHz was investigated and the prediction of the target strength by using the Kirchhoff-Ray Mode model (KRM model) was compared with target strength measurements. The results obtained are summarized as follows; 1. In the averaged target strength pattern for 13 large yellow croakers the maximum target strength was -35.13 dB at $-13.35^{\circ}$ on a tilted angle. 2. The relationship between fork length(L, cm) and averaged target strength(TS, dB) was expressed as follows; TS=23. 76log (L) -73.45 (r=0.47) TS=20log(L) -67.35 From this result, the conversion coefficient was -73.45 dB and 6.1 dB lower than the coefficient -67.35 dB where the value of the slope of the regression equation is forced to be 20. 3. Averaged target strength and a length conversion coefficient derived from a target strength histogram for 13 large yellow croakers of mean length 25.59 cm were -41.23 dB, -69.72 dB, respectively. 4. In the range of $$2;{\ll} L (fish length /{\lambda}(wave length);{\ll}40$$, the prediction of the averaged target strength by the KRM model increased gradually with the increasing of $L/{\lambda}$ and was lower than the measured target strength.

A Sensing Scheme Utilizing Current-Mode Comparison for On-Chip DC-DC Converter (온칩 DC-DC 변환기를 위한 전류 비교 방식의 센서)

  • Kim, Hyung-Il;Song, Ha-Sun;Kim, Bum-Soo;Kim, Dae-Jeong
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.4
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    • pp.86-90
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    • 2007
  • An efficient sensing scheme applicable to DC-DC converters is proposed. The output voltage of the DC-DC converter is fed back and converted to a current signal at the input terminal of the sensor to decide if it is in the tolerable range. The comparison is accomplished by a current push-pull action. With the embedded reference current in the sensor realized from the reference voltage. The advantages of the scheme lie in the fairly accurate and efficient implementation in terms of power consumption and chip size overhead compared with conventional voltage-mode schemes as the major parameter in converting voltage to current is determined by (W/L) aspect ratio of the core transistors. In this paper, a DC-DC converter of 5V output from battery range of 2.2V${\sim}$3.6V adopting the proposed sensing scheme is implemented in a 0.35um CMOS process to prove the validity of the scheme.

Analysis of Carbonyl Compounds using DNPH Cartridge with LC-MS (DNPH cartridge/LC-MS 방법에 의한 카르보닐화합물 분석에 관한 연구)

  • Cho Deok-Hee;Song Il-Seok
    • Journal of Korean Society for Atmospheric Environment
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    • v.22 no.2
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    • pp.201-208
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    • 2006
  • Several carbonyl compounds are important because of their irritant and toxic properties, mutagenicity and carcinogenicity. Therefore, they are regulated in korean odor emission standard. In this study, atmospheric pressure ionization-mass spectrometry(API-MS) is used for the analysis of carbonyl compounds after derivatization with 2, 4-dinitrophenylhydrazine (DNPH) and liquid chromatographic separation. In the negative ion mode, the $[M-H]^-$ pseudomolecular ions are most abundant for the carbonyls. Analytical parameters such as linearity, repeatability and minimum detection limit were evaluated. The linearities ($r^2$) for carbonyls were $0.9977{\sim}0.9999$ when analyte concentration ranges from $25\;to\;250{\mu}g/L$(n=6). The relative standard deviations (%RSD) for carbonyls were $0.55{\sim}3.51%$ for concentration of $100{\mu}g/L$(n=5). The minimum detection limit (MDL) was $1.88{\mu}g/L$(0.27 ppb) for i-valeraldehyde. It was shown that LC-MS method has a great potential for carbonyl compounds analysis.

Stress Intensity Factors for the Mixed Mode in Rotating Disks by Boundary Element Method (경계요소법에 의한 회전원판의 혼합 모우드 응력확대계수)

  • Park, S.O.
    • Journal of the Korean Society for Precision Engineering
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    • v.13 no.7
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    • pp.148-157
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    • 1996
  • This paper deals with the applicability of linear elastic fracture mechanics under centrifugal force. Stress intensity factors K are calculated as a function of the inclination crack of length 2a, the position at different angular velocities 1200rpm, 2400rpm and at different values of the inclination crack angle .phi. ( .phi. = 0 .deg. , 15 .deg. , 30 .deg. , 45 .deg. , 60 .deg. , 75 .deg. , 90 .deg. ) and are measured in models of rotation disks using a boundary element method. Especially, stress intensity factors $K_{l}$ and $K_{ll}$ obtained separately from the crack tip of the mixed mode, were used to further investigate the influence of $K_{l}$ and $K_{ll}$ on fracture in rotating disks. With the increase in the speed of rotation, the effect of K/ sub l/became larger where as that of $K_{ll}$ became small. For the increase in the inclination crack angle .phi. , a decrease in $K_{l}$ and an increase in $K_{ll}$ were observed.

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Xylitol의 생산성 향상을 위한 Two-stage Fed-batch 배양조건의 최적화

  • Jo, Yeong-Il;Seo, Jin-Ho;Yu, Yeon-U
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.356-359
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    • 2000
  • A two-stage fed-batch fermentation was carried out to increase xylitol productivity by Candida tropicalis. The first stage for cell growth was performed in the pH-stat and continuous fed-batch modes. The higher cell growth and lower ethanol production obtained in the fed-batch mode where the growth medium was fed when pH of culture broth increased over 5.7. And also the effect of oxygen transfer on xylitol production was investigated by changing agitation speed under 0.5 vvm of aeration. The maximum xylitol productivity and yield were obtained at 500 rpm of agitation.

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Design and Fabrication of a Multi-Function Circuit to Implement Hybrid-Conversion RF Front-End for Broadband and Multiband System (광대역 및 다중 대역 시스템용 혼성 변환 방식 RF 전단부 구현을 위한 다중 기능 회로의 설계 및 제작)

  • Go, Min-Ho;Ju, Young-Rim;Jo, Yun-Hyun;Park, Hyo-Dal
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.3
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    • pp.292-300
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    • 2010
  • In this paper, we propose a RF front-end architecture based on hybrid conversion which is available to receive both broadband and multiband DVB-H receiver, and a multi-function circuit for implementing the RF front-end is fabricated. A multi-function circuit is operated as a sub-harmonic mixer mode in the case of receiving a broadband VHF/UHF band, which show a conversion loss of -10.0 dB, noise figure of 7.0 dB and IIP3 of 2.0 dBm. On the other hand, it is performed as a attenuation mode with a insertion loss of -10.0 dB in receiving a multiband, L-band.