• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.36-36
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• 2014

Apple blotch, caused by Marssonina coronaria, induce early defoliation in apple and leading to critical economic losses in apple orchards in Korea. Since M. coronaria is difficult to culture, we developed isolation and cultural method. We collected M. coronaria isolates from Gyeongbuk Province and then constructed phylogentic tree based on ITS regions. As the results, phylogenetic relationship indicated that all Korean isolates formed a same cluster and closely related to Chinese isolates [1]. Ecological characteristic of M. coronaria have been observed in apple orchards which located in Gyeongbuk Province from 2011 to present. As the results, the typical apple blotch symptoms were observed from July, and then the infected leaves were discolored and formed acervuli on the leaves. After rainfall, severe infection of symptoms such as discoloration and early defoliation were continuously observed until October. Also overwintered conidia were observed in next March on the fallen diseased leaves [2]. In the last 5 years, ascopores of M. coronaria were not observed in apple orchards which were severely infected by M. coronaria in Korea. Thus, it is assumed that overwintered conidia could be a primary inoculum of M. coronaria. Meanwhile, apple blotch has long latent periods compare to other apple disease. During the latent period, early diagnosis of apple blotch is the most important to control the disease by spray fungicide. In this reason, we developed novel diagnostic method to detect M. coronaria during latent period using optical coherence tomography (OCT) and Loop-mediated isothermal amplification (LAMP) method [2, 3]. In this presentation, it will introduce ecological characterization of M. coronaria in Korea and unique detection technique of M. coronaria in apple. It will be helpful to develop new strategies to control apple blotch in Korea.

• 대한화학회지
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• 제44권6호
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• pp.547-555
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• 2000

대구${\cdot}$경북지역의 상수원 및 공업용수로서 사용되고 있는 낙동강 중${\cdot}$상류지역 하천 표류수의 수질을 분석하고, 하상 퇴적층에 함유된 중금속과 유기물의 종류 및 함유량을 분석하였다. 시료 채취는 갈수기 중 6월 22, 23 양일간 안동댐 상류 청량산 도립공원 양삼교 밑을 제 1지점으로 하여, 경북 지방을 통과하는 낙동강의 최하단인 고령군 고령교까지, 주로 공단의 전후 및 하천의 합류 지점을 대상으로 총 10개 지점에서 실시하였다. 표류수의 분석은 표류수의 시험항목 중 암모니아성 질소, 아질산성 질소, COD 등 총 22개 항목에 대하여 수행하였으며, 퇴적층은 trichloroethylene 등의 유기물과 중금속 등 11개 항목에 대하여 분석하였다. 퇴적물 시료는 용출시험법 및 산분해법으로 각각 분석하여 결과를 비교하였으며, 시료 중 중금속 성분은 유도결합 플라스마 방출분광법으로 분석하였고, 기타 성분은 환경부기준 공정시험방법에 의하여 분석하였다.

• 식물병연구
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• 제23권3호
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• pp.262-267
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• 2017

2015년부터 2016년까지 국내 5개 지역에서 기장 101점, 수수 200점의 시료를 채집하였다. 채집한 시료에 대해 paired-end RNA sequencing, RT-PCR 진단을 수행하였다. 그 결과, 경상도에서 채집한 수수에서 Rice black-streaked dwarf virus (RBSDV)가 검출되었으며, RBSDV, Rice stripe virus (RSV), Barley virus G (BVG), Cereal yellow dwarf virus (CYDV) 4종의 바이러스가 기장에서 검출되었다. 기장에서 검출된 4종의 바이러스 중 RSV와 RBSDV는 경상도에서 채집한 시료에서 높은 감염률을 보였다. 반면, BVG는 5개 지역에서 모두 감염이 확인되었으며, 이미 전국적으로 분포되어 있는 바이러스인 것으로 보인다. 본 연구에서는 국내 기장과 수수에서 RBSDV를 처음으로 동정하였으며, 수수에서 검출된 CYDV는 기존에 보고된 CYDV 분리주와 상동성이 상대적으로 낮은 것으로 보아 Polerovirus 속의 신종 바이러스로 예상된다.

• Mycobiology
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• 제39권3호
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• pp.200-205
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• 2011

Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.6% and 99.2% sequence similarity to each other in internal transcribed spacer regions and partial sequences of 28S rDNA, respectively. The isolates were phylogenetically closely related to Chinese isolates. Selected isolates did not differ in their pathogenicity. The optimum conditions for fungal growth were $20^{\circ}C$ and pH 6 on peptone potato dextrose agar (PPDA). Peptone and mannose were the best nitrogen and carbon source, respectively. Fungal growth was better on PPDA than on common potato dextrose agar. This study provides valuable information for integrated disease management program and facilitates the routine culturing of M. coronaria.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.139.2-140
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• 2003

Ornithogalum showing mosaic symptoms were collected from the isolated field of National Plant Quarantine Service in Sengrimmyon of Kyungnam province. Electron microscopic examination of negatively strained preparation was filamentous particle of 740nm in lenght. Indirect-ELISA determined that the virus was serologically related to potyvirus. A single major protein band of Mr 30,000 was observed after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Indicator plant test showed masaic, necrotic local lesion and sunken areas in leaves of Nicotiana clevelandii and Tetragonia expansa, while the others of indicator plants did not infect. An enzyme-aided purification protocal was used, which eliminated a highly viscous mucilage from extracts of the Omithogalum. Total RNA extracted from infected Omithogalum leaves were amplified of 411b.p fragment in reverse transcription (RT)-PCR when primers specilic for the coat protein gene. An isolate of Omithogalum mosaic virus (OrMV) of the genus Potyvirus was identified as the casual agent of the disease on the basis of electron microscopic, biological and serological reaction.

• Journal of Preventive Medicine and Public Health
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• 제23권1호
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• pp.87-97
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• 1990

1985년 4월 1일부터 1987년 3월 31일 사이에 경상북도 군위군에 거주하는 49세이하의 가임여성들과 1987년 3월 1일부터 1988년 2월 28일 사이에 경상남도 합천군에 거주하는 49세이하의 가임여성들 그리고 1985년 4월 1일부터 1987년 3월 31일 사이에 대구시 남구 모자보건센터에 분만을 위해 찾아온 모든 임산부들을 대상으로 이들에게서 태어난 출생아들을 두 군지역에서는 생후 1년간 그리고 모자보건센터에서 출생한 경우는 생후 27일간 추적하여 영아사망률과 신생아 사망률을 각각 조사하였다. 조사기간중에 군위군과 합천군에서 출생한 1,359명의 영아들 가운데 생후 1년 동안에 17명이 사망하여 농촌지역의 영아사망률은 출생아 1,000명당 12.5이었다. 총 영아사망중 신생아사망이 82.4% 그리고 신생아 후기 사망이 17.6%를 차지하였다. 대구시 남구 모자보건센터에 내원한 산모들에게 태어난 6,001명의 출생아들 가운데 4,834명(80.6%)만이 생후 27일까지 추적되었는데 이들 중 36명이 사망하여 신생아 사망률은 출생아 1,000명당 7,4명이었다. 추적된 산모와 추적 안된 산모의 특성 및 신생아 체중을 비교한 결과 현저한 차이를 나타내지 않았다. 신생아 후기 사망률의 비를 6 : 4로 가정했을 때 모자보건센터에서 출생한 영아의 영아사망률은 출생아 1,000명당 12.3명으로 추정되었다. 군위군과 합천군의 영아사망률과 대구시 남구 모자보건센터에서 관찰된 신생아사망률, 영아사망의 사망시기별 분포와 영아사망원인 그리고 저체중아출생률을 고려할 때 1985년부터 1988년 사이의 한국의 영아사망률은 출생아 1,000명당 12에서 15명일 것으로 추정되었다.

• 한국경제지리학회지
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• 제10권3호
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• pp.304-318
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• 2007

본 연구는 경상북도를 사례로 1990년대 말부터 추진한 재래시장 활성화 정책의 효과를 평가해보는데 주된 목적이 있다. 재래시장 활성화를 위해 추진한 시장의 시설현대화와 경영현대화사업의 효과에 대한 정성적 평가결과, 각각 그 효과는 미약한 것으로 나타났다. 이것은 기존의 재래시장 활성화 정책이 재래시장의 공간경쟁력 강화에 대한 고려가 부족하였기 때문으로 보인다. 이에 본 연구에서는 재래시장의 공간경쟁력을 강화하는데 역점을 둔 대안적인 재래시장 활성화 정책을 모색 제안하였다.

• 한국과학교육학회지
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• 제11권1호
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• pp.97-115
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• 1991

The purpose of this study is in making a important way for in-service training of science teacthers. So the study was carried out to analyze the text books of in-service training school in Gyeongnam, Kyungpook, Junpook province and Pusan city and to survey teacher's opinion by questonnaires collected from teachers in Gyeongnam province. The result abtained are as follows : 1. The contents of. tire text books for in-service training course have very critical defference at each province. 2. Most of teachers wanted to get more updating information of the science and more comprehensive knowledge of higher level' of science subject. 3. In order to obtain comprehensive basic concept, develop of inquiry ability, and promote of scientific attitude, the thinking experiment which is selected to experimental subjects of secondary school are need to be selected. 4. The new text-book model for in-service training course should be developed, and the finnancial support will be required to develop such a text.

• The Plant Pathology Journal
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• 제17권5호
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• pp.300-304
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• 2001

Apple is the most economically important fruit in Korea. The suspected viroid disease of dapple apple was found in apple fruits cultivated in Kyungpook province. Symptoms begin in mid-July as small circular spots, which stand out against the background color on the young fruit. Dappling of the fruit becomes more intense and easier to detect as the fruit approaches maturity; the affected spots remain yellowish as the fruit matures. no leaf or bark syndromes have been associated with this disease. The infected fruits are downgraded considerably during quality grading. The low molecular weight RNA containing viroid RNA molecules were extracted from the peels of the apples with dapple symptoms. The RNA molecules were extracted from the apples using Qiagen column chromatography. The purified RNAs were used for the synthesis of cDNA with RT-PCR. The PCR products were then ligated into a pGEM-T Easy vector, cloned and sequenced. The sequence of the viroid RNA molecule shows 331 nucleotides with one base difference ("G" insertion between the position of 133 and 134) compared with that of the Apple scar skin viroid (ASSVd) reported by Hashimoto and Koganezawa in Japan. This is the first report on the occurrence of the ASSVd in apple trees cultivated in Korea, as well as the identification of a new Korean strain of the ASSVd.the ASSVd.

• Parasites, Hosts and Diseases
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• 제56권3호
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• pp.287-290
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• 2018

Anaplasmosis is a tick-borne, non-contagious, zoonotic disease caused by Anaplasma spp., which include Anaplasma marginale, A. centrale, A. phagocytophilum, A. platys, A. ovis, and A. bovis. Recently, in Korea, the prevalence of Anaplasma spp. has been investigated in some animals, such as dogs, horses, goats, cats, and Korean water deer. In cattle, A. marginale is the most virulent species and regarded as the typical type of species. However, data on the seroprevalence of Anaplasma spp. in cattle in Korea during the last decade is limited. This study was designed to investigate the seroprevalence of bovine anaplasmosis in Korea. From 2010 to 2013, blood samples were collected from 568 cattle. Forty animals (7.0%) tested seropositive for Anaplasma spp. by cELISA. Despite that current bovine anaplasmosis seropositivity rate in the Gyeongsangbuk-do is lower than those in tropical countries, anaplasmosis needs to be regarded as a concerning disease. The identification of the specific Anaplasma species infecting cattle in this province requires additional molecular studies. Moreover, further monitoring and control programs for bovine anaplasmosis is required, and the information from this study will be beneficial to develop these programs.