• Journal of the Korea Institute of Building Construction
• /
• v.16 no.1
• /
• pp.59-65
• /
• 2016

This study examined the compressive strength development and pH values of alpha-calcium sulfate hemihydrate(${\alpha}-CH$)-based binders developed for vegetation concrete with neutral pH between 6~7. Considering cost down and strength enhancement of the prepared binders, the ${\alpha}-CH$ was partially replaced by ground granulated blast furnace slag(GGBS), fly ash(FA), or ordinary Portland cement(OPC) by 25% and 50%. The compressive strength of mortars using 100% ${\alpha}-CH$ was 50% lower than that of 100% OPC mortars. With the increase of the replacement level of GGBS or FA, the compressive strength of ${\alpha}-CH$-based mortars tended to decrease, whereas the pH values were maintained to be 6.5~7.5. The main hydration products of ${\alpha}-CH$-based binders with GGBS or FA were a gypsum($CaSO_4$), whereas portlandite($Ca(OH)_2$) was not observed in such binders. Meanwhile, the pH values of ${\alpha}-CH$-based binders with OPC exceeded 11.5 due to the formation of $Ca(OH)_2$ phase as a hydration product. From the thermogravimetric analysis, the amount of $Ca(OH)_2$ in ${\alpha}-CH$-based binders with OPC was evaluated to be approximately 10% of the cement content.

• Journal of Embryo Transfer
• /
• v.30 no.3
• /
• pp.121-128
• /
• 2015

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed ($37^{\circ}C$) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at $38.5^{\circ}C$ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

• Journal of Embryo Transfer
• /
• v.29 no.4
• /
• pp.339-344
• /
• 2014

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. Therefore, we applied CRISPR/Cas9 system to generate FoxN1-targeted pig parthenogenetic embryos. Using single guided RNA targeted to pig FoxN1 genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. In results, regardless of the concentrations of vector, the cleavage rate were significantly (p<0.05) decreased ($4ng/{\mu}l$, 51.24%; $8ng/{\mu}l$, 40.88%; and $16ng/{\mu}l$; 45.22%) compared to no injection group (70.44%). The blastocyst formation rates were also decreased in vector injected 3 groups ($4ng/{\mu}l$, 7.96%; $8ng/{\mu}l$, 6.4%; and $16ng/{\mu}l$; 9.04%) compared to no injection group (29.07%). In addition, the blastocyst formation rates between sham injected group (13.51%) and no injection group (29.07%) also showed significant difference (p<0.05). The mutation rates were comparable between groups ($4ng/{\mu}l$, 18.4%; $8ng/{\mu}l$, 12.5%; and $16ng/{\mu}l$; 20.0%). The sequencing analysis showed that blastocysts derived from each group were successfully mutated in FoxN1 loci regardless of the vector concentrations. However, the deletion patterns were higher than the patterns of point mutation and insertion regardless of the vector concentrations. In conclusion, we described that cytoplasmic microinjection of FoxN1-targeted CRISPR/Cas9 vector could efficiently generate transgenic pig parthenogenetic embryos in one-step.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.2
• /
• pp.142-146
• /
• 1989

Anther and/or tissue culture of cross pollinated crops would be very important because it can result in the direct use of haploids or doubled haploids for developing superior hybrids or varieties. The objective of the study was to investigate the response frequencies in anther and/or tissue-cultured hybrids of corn. pearl millet and buckwheat to identify agronomically acceptable germplasm of the crops. 27 crosses of corn inbred lines were evaluated by plating their anthers on N6. MS and Yu-Pei media. Two genotypes of FR1l41/FR16 hybrid cultured on N6 medium and Fla 2BT73/S6013 hybrid cultured on N6 medium responded with one anther producing calli when plated after 5$^{\circ}C$ low temperature treatment for one week. Immature embryos of corn hybrid Suwon 19 responded producing calli that were regenerated to plants at a 8.6 percent success rate. Of the 20 corn hybrids. immature tessels of FR1l41/FR16. B68/A1l6N//KS15. KS16/KS17. GA209/DB578 and SDB126/GA209 crosses responded at a relatively higher success rate producing calli that were regenerated to plants. In tissue culture of elongating culms of pearl millet x Napier grass interspecific hybrid. 2.5-4.0mm long pieces of the culm were good for callus induction resulting in higher success rate. The epicotyl of buckwheat was very good for tissue culture. and the node produced the plants regenerated directly without callus induction on the B5 medium containing I ppm BA and 0.05 ppm IBA. There were great differences in response to anther and/or tissue culture of corn, pearl millet and buckwheat due to genotype x medium and environment interactions.

• Journal of Korean society of Dental Hygiene
• /
• v.6 no.4
• /
• pp.455-467
• /
• 2006

Demand for appropriate health care has gradually increased in Korea. In addition, developments of community- and school-based oral health programs have also focused oral health care for the oral health promotion. Especially, school-based oral health programs are the underpinnings of promoting oral health and preventing oral diseases among schoolchildren. School-based oral health programs have had three major components: oral health education, oral health services, and a healthful environments. These included oral health education(one-to-one communication, group communication, and use of mass communication), oral examination, fluoride mouthrinsing, pit-and-fissure sealants, fluoride gel application, mechanical plaque control, and chewing xylitol candy. In this study, we evaluate the effects of oral health programs among primary schoolchildren by comparing the oral health knowledge, oral health behaviors, and perception of caries prevention procedures. Data for this study were obtained from 699 primary schoolchildren at the two primary school in Daegu, Korea. One is experimental group, N primary school, that was established school-based oral health center under supervision of Nam-gu Public Health Center, the other is control group, N' primary school, that was yet to establish school-based oral health center. We surveyed children's oral health knowledge and behaviors, and perception of caries prevention procedures using self-administrated questionnaire and then analyzed differences of each item among two groups. The brief findings of this study were summarized as follows. There are several advantage to a comprehensive school-based oral health program. (1) School-based oral health programs facilitate and increase the effectiveness of teaching oral health subjects. (2) Schoolchildren are available for prevention or treatment procedure. (3) School-based oral health center may be less threating than private dental clinic. (4) With comprehensive school-based oral health programs the decayed, missing, and filled teeth(DMFT) of schoolchildren should demonstrate a substantial and steady decrease over time(Choi et al, 2004). In conclusion, treatment is not the answer to solving children's oral health programs; rather primary prevention is the key. Many countries and communities are focusing on hoe millions of underprivileged children can be provided with health care. Schoolchildren gain the knowledge and behaviors to attain and maintain good oral health in schools. For these reasons, the role of school-based oral health center is not only important but also a necessity.

• Tunnel and Underground Space
• /
• v.16 no.3 s.62
• /
• pp.251-258
• /
• 2006

In this paper, the applicability to the Korean rock condition of using the deformation moduli based on Rock Mass Rating (RMR) and Pressuremeter Test (PMT) is evaluated. The correlations among deformation moduli and various rock properties were also analyzed. It appears that the existing correlations using RMR overestimate the deformation moduli and wide variation was found between predicted moduli using these correlations and measured values. As for the correlations among the deformation moduli and various rock properties, Rock Quality Designation (RQD) and unconfined compressive strength (UCS) were found to correlate to deformation moduli reasonably well, but joint spacing and joint conditions appear to correlate poorly to RQD and UCS. Additionally, groundwater can not be correlated with the modulus values. While the depth has very little contribution to deformation modulus, it should be factored in the simple regression analyses with various rock mass properties, especially with the correlations made with UCS, RQD etc. With the deficiencies of these correlations, more in depth analysis techniques such as multivariate correlations may be to reliably estimate deformation modulus of rock mass.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2012.05a
• /
• pp.881-881
• /
• 2012

낙동강유역에는 4대강 사업으로 8개의 보가 낙동강 본류에 새롭게 설치되었으며, 따라서 상류로부터 유입되는 유사가 보상류에 퇴적되어 하천제방이나 하천구조물의 안전을 위협할 수 있다. 또한 홍수시에는 농경지의 매몰과 저수지의 퇴적현상이 발생하는 등 유역유사에 의한 다양한 문제가 발생할 수 있으며, 강우로부터 유출되는 유사는 강우사상에 따라 빠르게 변할 수 있으나 수체에 즉각적인 영향을 유발하지 않을 경우 만성적인 특성으로 인해 침전물을 계속 이동시키며 축적되는 문제가 발생한다. 따라서 이러한 문제를 사전에 예측하고 낙동강유역에 대한 유역유사관리 대책마련을 마련하기 위해 유역유출과 유사의 이송 및 퇴적양상을 분석하여 유역관리를 위한 자료를 구축할 필요가 있다. 이러한 기초자료 분석을 위해 활용되는 유역모형 중 SWAT(Soil and Water Assessment Tool) 모형은 장기간 유역 유출로 인한 유사 모의를 수행하는데 가장 많이 활용되는 모형이며, 미국 농무성 농업연구소(USDA Agricultural Research Service, ARS)의 Jeff Amold 등에 의해 개발된 모형이다. SWAT 모형은 장기유출 및 유사 발생에 대한 시 공간적 변화를 모의하는 모형으로 넓은 범위의 유역에 대해 일단위 모의 간격으로 최대 100년까지 모의가 가능한 장기유역 모의에 최적화된 모형이다. 본 논문에서는 SWAT 모형을 이용하여 낙동강유역의 장기 유역 유출 및 유사 모의를 수행하기위해 2004년에서 2009년까지의 유출량 자료와 유량-유사량관계곡선 등을 이용하여 본류 및 지류 유출구 주요지점에 대해 검보정을 수행하고자 한다. 모의의 정확도 향상을 위해 안동댐, 임하댐, 영천댐, 합천댐, 남강댐, 밀양댐 및 낙동강 본류 등 총 7개 유역으로 구분하여 SWAT 모형을 구축하였으며, 각 유역별로 유출량과 유사량에 대하여 각각 보정 및 검증을 수행하였다. 낙동강 본류유역 총 7개의 댐 방류량자료와 20개의 대형하수종말처리장 방류량자료를 이용한 낙동강유역의 유출량 보정 및 검증 결과, 모의치가 실측치를 잘 반영하고 있는 것으로 나타났다. 또한 총 11개 지점의 수위관측소에 대해 유사량 측정 성과를 이용한 유사량 보정 및 검증 결과, 강우시 유사량 모의값은 유출량-유사량관계곡선 식을 잘 반영하였으나, 비강우시에는 모의값이 관계곡선 식의 결과값보다 대체적으로 높게 나타났다.

• Korean Journal of Soil Science and Fertilizer
• /
• v.34 no.2
• /
• pp.98-104
• /
• 2001

Cultivation area of the plastic film-house has been continuously increased with the increase of consumers' income. Intensive land use without fallowing or crop rotation caused severe problem such as salt accumulation in soils and in turn retarded growth and low productivity. This study was carried out to solve them derived from longterm intensive farming practices. Seven farmers who are practicing plastic film-house cultivation were recommended for case study by municipal government and selected for their excellency of cultivation and soil management. The cultivation periods of these systems were in the range of 5 to 40 years in the regions mainly located in alluvial soil cultivated with cucumber, tomato and red pepper. The soils texture of the excellent farmers' fields were silt loam or sandy loam, ranged from 7 to 15 percents of clay contents. Soil bulk density, depth of plowing layer and soil aggregates contents of the farmers' soils were 0.89, 23.1 cm, 61.6% whereas those in neighboring soils were 1.10, 17.8 cm, 54.2 %, respectively. And pH, OM and $NO_3-N$ of the farmers' soils also were better than those of neighboring soils. There was no difference in population densities of nematode between the good farmers' and neighboring soils, but actinomyces and Fusarium densities of recommended farmers' soils were better than neighboring soils. The major farming practices by the good farmers were characterized by deep plowing with flooding, amendment of crude organic matter, and reduction of chemical fertilizer application before transplanting, and also drip irrigation and liquid manure application after planting. They also conducted solar sterilization with or without flooding, removal of plastic films during rainy days and culturing rice or corn as rotation crops to avoid the problems mentioned above.

• Journal of Korea Water Resources Association
• /
• v.40 no.1 s.174
• /
• pp.11-24
• /
• 2007

There exist various difficulties in runoff analysis due to many ungauged basins in Korea and the runoff phenomena is also more and more complicated by the change of geologic characteristics due to the urbanization. So, we use GIS technique which is widely used in hydrologic field and cell runoff concept for the fast and effective runoff simulation. This study uses the observations of 6 stage stations in Wi-Cheon watershed and simulates the watershed parameters by using WMS model. We construct DEM by the grids which are consisted based on the criteria of minimum area according to land use. The cell runoff is estimated by an average weighted method using mean annual streamflow and mean maximum daily streamflow obtained from six stage stations. The runoff ratio at arbitrary site is estimated by conducting the direction analysis of streamflow and by removing sinkhole. We compare the simulated and observed runoffs and know that the simulated runoff shows the valid results. So, we could use the geographical information and cell runoff concept for more fast and effective runoff simulation studies.

• Journal of Veterinary Clinics
• /
• v.32 no.6
• /
• pp.519-522
• /
• 2015

A 6-year-old castrated male Shih-Tzu was admitted to the Gyeongsang Animal Medical Center with hematuria and vomiting. Serum chemistry indicated increases in blood urea nitrogen and creatinine values. Radiography showed radiopaque materials attached to the ventralis of the inner bladder wall. Penile urethra also was filled with radiopaque materials. Ultrasonography revealed bilateral perineal effusion in the kidney, thinned bladder wall, and materials with acoustic shadowing at the caudal extremity of the os penis. Because urination was not identified by urinary obstruction, cystocentesis was performed. Hematuria was diagnosed by urinalysis, but clear crystals were not identified. Owing to failure of securing patency to the bladder using retrohydropropulsion, cystotomy and urethrotomy with retrohydropropulsion were performed. In this report, we describe the clinical and radiographic findings of urethral plugs in a male Shih-Tzu dog, wherein the urethral plugs were treated successfully with hydropropulsion, cystotomy, and urethrotomy. It provides an option for the treatment of urethral plugs in a dog.