• 한국산림과학회지
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• 제96권3호
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• pp.235-244
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• 2007

본 연구는 교토의정서에서 인정하고 있는 조림 CDM을 북한 지역에 적용하였을 때의 경제적 타당성을 분석하였다. 기존의 북한 산림면적 추정에 관한 연구결과를 활용하여 조림 CDM을 적용할 수 있는 잠정 대상 면적을 추정한 결과 북한에는 조림 CDM 사업이 가능한 황폐임지가 약 51만 ha가 존재하는 것으로 추정되었으며, 경제적 타당성 검토 대상지로 선정된 개성지역에는 약 8,000 ha의 CDM조림사업이 기능한 황폐임지가 존재하는 것으로 나타났다. 개성지역의 산림황폐지에 북한의 주요조림수종 가운데 하나인 아까시나무(Robinia pseudoacacia)를 인공조림하고 20년 동안 유지함으로써 기존의 토지이용에 비하여 탄소고정을 증가시키는 사업을 조림 CDM사업으로 설정하였다. 수목의 탄소흡수량을 추정하고 사업을 시행하는데 필요한 산림조성 비용, 사전행정비용, 배출권 관련 행정비용을 포함하는 비용을 분석함으로써 조림 CDM사업의 경제적 효과성을 평가하였다. 개성지역 황폐임지에 아까시나무를 조림하여 20년 동안 유지하는 CDM 사업을 통해서 흡수할 수 있는 이산화 탄소량은 ha당 약 $376\;tCO_2$로 추정되었으며 배출권 판매 시나리오별로 생산할 수 있는 배출권량은 총 사업기간을 통해 tCER이 503 tCER/ha, lCER이 265 lCER/ha로 나타났다. 총 투입된 비용을 기준으로 tCER 한 단위를 생산하는 데 투입된 비용은 US$ 4.04로 나타났고 lCER 한 단위를 생산하는 데는 US$ 7.67로 나타났다. 그러나 tCER과 lCER은 시장 가격이 다를 수 있기 때문에 단순히 배출권량만으로 그 경제적 수익성에 있어서의 우위를 가름하기는 힘들다.

• 에너지공학
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• 제18권4호
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• pp.246-257
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• 2009

기후변화와 관련 국제사회가 안고 있는 과제는 개도국의 참여가 없이 선진국 중심의 온실가스 감축만으로는 전 지구의 온실가스 농도안정화 목표 달성이 불가능하다는 인식이다. 특히 교토의정서 체계의 한계를 극복하고 개도국을 참여시키기 위한 방안으로 부문별 접근방식이 제기되고 있다. 이에 따라 본 논문에서는 우선 그동안 제기되어 왔던 부문별 접근방식의 종류, 적용기준, 범위, 유용성 등에 대한 논의 요지를 정리하여, post-2012 체제하에서 협상대안으로서의 부문별 접근방식의 잠재력을 살펴 보았다. 또한, 앞으로 부문별 접근방식이 협상대안으로 구체화될 경우에 대비, 우리나라 산업에서의 부문별 접근방식 적용 여건을 분석하였다. 이를 토대로 세계 평균 이상의 에너지 효율을 실현하고 있는 업종은 기술 기반 부문별 접근방식, 세계 평균 수준의 에너지 효율을 보이는 업종은 부문별 크레딧 메카니즘 혹은 지수기반 혹은 부문 전체 국가간 접근을 검토대상으로 제시하고 있으며, 향후 부문별 접근방식채택 및 이행을 위한 향후 연구과제를 제시하고 있다.

• 한국건설관리학회논문집
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• 제16권2호
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• pp.65-76
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• 2015

청정개발체제(CDM) 사업은 신재생에너지사업의 보조를 통해 지구온난화 가스의 감축을 꾀하는 대표적인 국가 및 기업 간 배출권 거래(cap and trade)제도이다. 재래식 발전 방식에 비해 수익성이 낮은 태양광, 풍력, 수력 등의 사업이 CDM 사업으로 승인을 받으면 매년 탄소배출권(CER)을 제공받고, 이의 판매를 통해 발생한 추가 수익으로 인해 사업 타당성이 향상될 수 있다. 그러나 CDM 사업으로 인정받기 위해서는 환경적, 기술적, 경제적 추가성(Additionality)를 입증해야 하는데, 해당 적용 기술, 베이스라인 측정 방법론, 온실 가스 감축량, 사업 내부 수익률(IRR) 등 다수의 변수에 따라 결과가 달라지기 때문에 사전적으로 승인여부를 파악하기가 어렵다. 본 연구에서는 신재생에너지로 분류되는 수력 사업의 CDM 승인여부를 예측할 수 있는 모델을 개발하는 것을 목표로 하였다. 구체적으로 UNFCCC에서 제공하는 수력 사업 데이터를 활용하여 로그 회귀분석 및 CART 분석을 실시하여 예측모델을 개발하였으며 이와 함께 승인 여부에 유의하게 영향을 미치는 핵심 인자들을 파악하였다. 구축된 로그 회귀 및 CART 예측모델은 AUC가 각각 0.7674 및 0.7231로 예측 정확성이 비교적 높게 나왔다. 또한 수력 사업에서는 온실가스 저감량 대비 투자액, 시간당 발전량 및 내부수익률이 승인여부에 유의한 변수들로 파악되었고, 이에 비해 특정 기술이나 측정 방법론은 영향이 없는 것으로 드러났다. 즉, 특정 기술을 불문하고 온실가스를 투자 대비 가장 효율적으로 저감하는 사업과 수력사업들 중 상대적으로 소규모로 진행되는 사업이 CDM 사업으로 승인될 가능성이 높다는 것으로 해석된다.

• 산학경영연구
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• 제19권2호
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• pp.69-94
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• 2006

매립된 패기물의 분해과정에서 생성되는 부산물로서의 매립가스는 악취와 환경오염을 유발하는 유해한 가스로 여겨져 왔다. 그러나 발열량이 $5,000kcal/m^3$에 달하는 매립가스의 특성은 에너지원으로써의 활용가치가 탁월한데다 지구온난화의 주범으로 떠오르면서 그 회수 필요성이 더욱 강조되고 있어, 재생에너지로서의 매립가스를 대체에너지 자원으로 이용하려는 추세는 매우 고무적인 현상이라 하겠다. 현재 우리나라의 매립가스자원화 사업은 가스엔진을 이용한 전력생산이 대부분을 차지하고 있으며, 부분적으로 중질가스와 고질가스의 활용에도 관심을 보이고 있다. 가스발전기의 가동률과 전력단가의 변동을 고려하여 매립가스의 경제성을 분석해본 결과, 초기 투자비 회수와 지속적인 수익성 확보를 위해서는 최소한 10년 이상의 발전사업 운영 및 전력판매 단가의 현실화가 요구되고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1458-1468
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• 2019

Objective: As one of the most important metabolic organs, the liver plays vital roles in modulating the lipid metabolism. This study was to compare miRNA expression profiles of the Large White liver between two different developmental periods and to identify candidate miRNAs for lipid metabolism. Methods: Eight liver samples were collected from White Large of 70-day fetus (P70) and of 70-day piglets (D70) (with 4 biological repeats at each development period) to construct sRNA libraries. Then the eight prepared sRNA libraries were sequenced using Illumina next-generation sequencing technology on HiSeq 2500 platform. Results: As a result, we obtained 346 known and 187 novel miRNAs. Compared with the D70, 55 down- and 61 up-regulated miRNAs were shown to be significantly differentially expressed (DE). Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis indicated that these DE miRNAs were mainly involved in growth, development and diverse metabolic processes. They were predicted to regulate lipid metabolism through adipocytokine signaling pathway, mitogen-activated protein kinase, AMP-activated protein kinase, cyclic adenosine monophosphate, phosphatidylinositol 3 kinase/protein kinase B, and Notch signaling pathway. The four most abundantly expressed miRNAs were miR-122, miR-26a and miR-30a-5p (miR-122 only in P70), which play important roles in lipid metabolism. Integration analysis (details of mRNAs sequencing data were shown in another unpublished paper) revealed that many target genes of the DE miRNAs (miR-181b, miR-145-5p, miR-199a-5p, and miR-98) might be critical regulators in lipid metabolic process, including acyl-CoA synthetase long chain family member 4, ATP-binding casette A4, and stearyl-CoA desaturase. Thus, these miRNAs were the promising candidates for lipid metabolism. Conclusion: Our study provides the main differences in the Large White at miRNA level between two different developmental stages. It supplies a valuable database for the further function and mechanism elucidation of miRNAs in porcine liver development and lipid metabolism.

• Animal Bioscience
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• 제34권11호
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• pp.1739-1748
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• 2021

Objective: In recent years, long noncoding RNAs (lncRNAs) have been identified in many species, and some of them have been shown to play important roles in muscle development and myogenesis. However, the differences in lncRNAs between Kazakh cattle and Xinjiang brown cattle remain undefined; therefore, we aimed to confirm whether lncRNAs are differentially expressed in the longissimus dorsi between these two types of cattle and whether differentially expressed lncRNAs regulate muscle differentiation. Methods: We used RNA-seq technology to identify lncRNAs in longissimus muscles from these cattle. The expression of lncRNAs were analyzed using StringTie (1.3.1) in terms of the fragments per kilobase of transcript per million mapped reads values of the encoding genes. The differential expression of the transcripts in the two samples were analyzed using the DESeq R software package. The resulting false discovery rate was controlled by the Benjamini and Hochberg's approach. KOBAS software was utilized to measure the expression of different genes in Kyoto encyclopedia of genes and genomes pathways. We randomly selected eight lncRNA genes and validated them by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: We found that 182 lncRNA transcripts, including 102 upregulated and 80 downregulated transcripts, were differentially expressed between Kazakh cattle and Xinjiang brown cattle. The results of RT-qPCR were consistent with the sequencing results. Enrichment analysis and functional annotation of the target genes revealed that the differentially expressed lncRNAs were associated with the mitogen-activated protein kinase, Ras, and phosphatidylinositol 3-kinase (PI3k)/Akt signaling pathways. We also constructed a lncRNA/mRNA coexpression network for the PI3k/Akt signaling pathway. Conclusion: Our study provides insights into cattle muscle-associated lncRNAs and will contribute to a more thorough understanding of the molecular mechanism underlying muscle growth and development in cattle.

• Animal Bioscience
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• 제36권1호
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• pp.144-155
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• 2023

Objective: Adipocyte differentiation is regulated by a variety of functional genes and noncoding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear. Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods: The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened and gene ontology (GO) and Kyoto encyclopedia for genes and genomes (KEGG) analyses were performed. Gain-of-function and loss-of-function combined with oil red O staining, Bodipy staining, and quantitative reverse-transcription polymerase chain reaction (qPCR) were utilized to determine the effect of miR-133a-3p on adipocyte differentiation. Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and CCAAT enhancer binding protein α (C/EBPα) and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (p<0.01), the loss-of-function also led to a significant down-regulation of peroxisome proliferator activated receptor gamma (PPARγ) (p<0.01). Conclusion: This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

• 대한한의학회지
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• 제44권2호
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• pp.106-118
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• 2023

Objectives: Network pharmacology is a method of constructing and analyzing a drug-compound-target network to predict potential efficacy and mechanisms related to drug targets. In that large-scale analysis can be performed in a short time, it is considered a suitable tool to explore the function and role of herbal medicine. Thus, we investigated the potential functions and pathways of Chongmyunggongjin-dan (CMGJD) on Alzheimer's disease (AD) via network pharmacology analysis. Methods: Using public databases and PubChem database, compounds of CMGJD and their target genes were collected. The putative target genes of CMGJD and known target genes of AD were compared and found the correlation. Then, the network was constructed using Cytoscape 3.9.1. and functional enrichment analysis was conducted based on the Gene Ontology (GO) Biological process and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways to predict the mechanisms. Results: The result showed that total 104 compounds and 1157 related genes were gathered from CMGJD. The network consisted of 1157nodes and 10034 edges. 859 genes were interacted with AD gene set, suggesting that the effects of CMGJD are closely related to AD. Target genes of CMGJD are considerably associated with various pathways including 'Positive regulation of chemokine production', 'Cellular response to toxic substance', 'Arachidonic acid metabolic process', 'PI3K-Akt signaling pathway', 'Metabolic pathways', 'IL-17 signaling pathway' and 'Neuroactive ligand-receptor interaction'. Conclusion: Through a network pharmacological method, CMGJD was predicted to have high relevance with AD by regulating inflammation. This study could be used as a basis for effects of CMGJD on AD.

• Animal Bioscience
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• 제36권9호
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• pp.1336-1349
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• 2023

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC]=0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.

• Animal Bioscience
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• 제37권5호
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• pp.929-943
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• 2024

Objective: The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis. Methods: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation. The differentially expressed genes were explored through deep RNA sequencing. Results: The highest mRNA level was found in omasum, subcutaneous fat, and intramuscular fat. Moreover, the highest mRNA level was found in adipocytes at day 4 of differentiation. The results of red-oil o staining showed that overexpression (Ad-FGF10) of the FGF10 gene significantly (p<0.05) reduced the lipid droplets and TG content, and their down-regulation (siFGF10) increased the measurement of lipid droplets and TG in differentiated bovine adipocytes. Furthermore, the overexpression of the FGF10 gene down regulated the mRNA levels of adipogenic marker genes such as CCAAT enhancer binding protein alpha (C/EBPα), fatty acid binding protein (FABP4), peroxisome proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), and Fas cell surface death receptor (FAS), similarly, down-regulation of the FGF10 gene enriched the mRNA levels of C/EBPα, PPARγ, FABP4, and LPL genes (p<0.01). Additionally, the protein levels of PPARγ and FABP4 were reduced (p<0.05) in adipocytes infected with Ad-FGF10 gene and enriched in adipocytes transfected with siFGF10. Moreover, a total of 1,774 differentially expressed genes (DEGs) including 157 up regulated and 1,617 down regulated genes were explored in adipocytes infected with Ad-FGF10 or Ad-NC through deep RNA-sequencing. The top Kyoto encyclopedia of genes and genomes pathways regulated through DEGs were the PPAR signaling pathway, cell cycle, base excision repair, DNA replication, apoptosis, and regulation of lipolysis in adipocytes. Conclusion: Therefore, we can conclude that the FGF10 gene is a negative regulator of bovine adipogenesis and could be used as a candidate gene in marker-assisted selection.