• 한국미생물·생명공학회지
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• 제29권3호
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• pp.134-141
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• 2001

경기도 일대의 가금류 공장부근 토양로부터 단백질 분해활성이 높은 균주를 분리한 후 이것들 가운데 Keratinolytic protease 생산성이 우수한 균주는 KP-364를 선별하여 효소생산을 위한 최적 배양조건을 검토하였다. 선별된 KP-364 는 형태학적 생화학적 특성을 종합한 결과 Pseudomonas sp 로 동정되었으며 편의상 Pseudomonas sp. KP-364로 명명하였다. 효소생산을 위한 최적 배지조성은 Keratin 0.1% glucose 2.0% soybeam meal 0.5% NaNO3 0.5% KCI 0.2%이었고 초기 pH 6.5 배양온도 $37^{\circ}C$, 48시간진탕 배양할때 효소생산이 가장 우수하였으며 이때의 효소의 역가는 1,270 U/ml/hr이었다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2002년도 금형가공 심포지엄
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• pp.11-18
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• 2002

The STD11 and KP4 are important steels and applied to the manufacturing of the die and mold. The purpose of this study is to investigate the machinability of tool steels of STD11(HRC60) and KP4(HRC32) when machining them by using ball end milling tools coated with TiAlN. Cutting forces by using a Kistler piezo-cell type tool dynamometer, surface roughness and tool wear by using tool microscope are used in the tests. The results from the cutting tests of KP4 specimens show that 85m/min. of cutting speed and 0.32mm/rev. of feed per revolution are optimum conditions for the higher productivity and 0.26mm/rev. with the same cutting speed are optimum conditions for better surface finishing. The results from machining STD11 workpiece at 30m/min. of cutting speed and 0.17m/rev. of feed per revolution show recommended for the higher productivity. The KP4 shows relatively smaller cutting forces than STD11 and STD11 shows the better surface finish than KP4.

• 약학회지
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• 제45권5호
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• pp.506-512
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• 2001

Ketoprofen (KP) was formulated as a transdermal patch using the percutaneous penetration enhancers sorbitan monmmleate(SMO), polyvinylpyrrolidone(PVP). The control patch without penetration enhancers showed a KP flux of 8.9$\pm$0.75$\mu\textrm{g}$/$\textrm{cm}^2$/h The flux was increased in proportion to the concentration of SMO added. Furthermore, lag times were decreased upon addition of SMO. Conversely; the skin flux of KP was decreased in proportion to the concentration of PVP added. Pharmacokinetic parameters including $C_{max}$, $T_{max}$, and AUC were increased when SMO was added. However, $C_{mas}$ significantly decreased by the addition of PVP. $T_{max}$ was not significantly different in 2%, 4%, and 8% PVP patches. Patches containing 4% PVP showed the highest AUC value (19.158$\mu\textrm{g}$.h/ml). We found that the effectiveness of the two percutaneous penetration enhancers for topical KP patches was similar, with the addition of appropriate amounts of HPC modifying both skin flux and lag time of KP in the patches. In conclusion, it is possible to manufacture KP patches exhibiting high AUC, high skin flux, and short lag time using percutaneous penetration enhancers of SMO and PVP.

• 천문학회보
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• 제37권2호
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• pp.123.1-123.1
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• 2012

We have compared near-real time Kp forecast models based on neural network (NN) and support vector machine (SVM) algorithms. We consider four models as follows: (1) a NN model using ACE solar wind data; (2) a SVM model using ACE solar wind data; (3) a NN model using ACE solar wind data and preliminary kp values from US ground-based magnetometers; (4) a SVM model using the same input data as model 3. For the comparison of these models, we estimate correlation coefficients and RMS errors between the observed Kp and the predicted Kp. As a result, we found that the model 3 is better than the other models. The values of correlation coefficients and RMS error of the model 3 are 0.93 and 0.48, respectively. For the forecast evaluation of models for geomagnetic storms ($Kp{\geq}6$), we present contingency tables and estimate statistical parameters such as probability of detection yes (PODy), false alarm ratio (FAR), bias, and critical success index (CSI). From a comparison of these statistical parameters, we found that the SVM models (model 2 and model 4) are better than the NN models (model 1 and model 3). The values of PODy and CSI of the model 4 are the highest among these models (PODy: 0.57 and CSI: 0.48). From these results, we suggest that the NN models are better than the SVM models for predicting Kp and the SVM models are better than the NN models for forecasting geomagnetic storms.

• 한국컴퓨터정보학회논문지
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• 제27권4호
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• pp.53-60
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• 2022

최근 드론으로 수집되는 데이터의 양이 급증함에 따라, 데이터를 안전하고 효율적으로 저장 및 처리할 수 있는 클라우드 컴퓨팅 기술의 지원이 필요하다. 하지만, 드론에서 통신하는 데이터를 공격자로 인해 탈취되거나, 유출, 위·변조 등 다양한 보안 취약점이 발생할 수 있다. 따라서 드론으로부터 수집되어 전송되는 데이터에 보안 기술이 필요하다. 다양한 보안 기술 중 속성기반암호인 KP-ABE 기법은 데이터 암호화와 사용자 접근 제어, 두 가지 특성을 만족시키는 보안 기술이다. 본 논문은 KP-ABE 기법을 연구하고, 이를 드론 환경에 활용하여 안전한 데이터 접근 제어 기법을 제안하고자 한다. 본 제안 기법은 드론 환경에서 통신되는 데이터의 기밀성과 무결성 및 안전한 접근 제어와 가용성이 제공된다. 그리고 KP-ABE 기법에서 제공해야 할 요구사항 중 빠른 암호문 탐색 및 일정 크기 암호문 출력을 제공한다.

• 미생물학회지
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• 제54권2호
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• pp.152-154
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• 2018

Klebsiella pneumoniae는 그람 음성균에 속하고 막대 형태를 가지며 인간이나 동물의 폐에 감염하여 병을 일으키는 균이다. K. pneumoniae는 흔히 항생제 내성을 나타내는데 이로 인해 항생제를 통한 치료가 어려워지게 된다. 이런 상황에서 숙주 균에 특이적이고 민감하게 반응하는 박테리오파지는 항생제 내성균의 치료에 대한 대체적인 접근법으로 제안될 수 있다. 박테리오파지 KP1은 하수처리장에서 분리되었으며 K. pneumoniae에 대해 특정적인 감염성이 있다. 본 연구에서는 Klebsiella pneumoniae 박테리오파지 KP1의 유전체 초안 분석을 수행하였다. KP1의 유전체 초안은 167,989 bp의 길이, 39.6%의 G + C 비율로 구성되어있다. 295개의 예측된 ORF들과 14개의 tRNA 유전자를 가지고 있다. 또한 이들은 lysozyme, 그리고 holin과 같은 다양한 세포 용해 관련 효소들을 포함하고 있다.

• Bulletin of the Korean Chemical Society
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• 제29권6호
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• pp.1141-1148
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• 2008

Geometry optimizations and electronic structure calculations are reported for meso-tetraphenyl porphyrin (TPP) and a series of meso-substituted catecholic porphyrins (KP99150, KP99151, KP99152, KP99153, and KP99090) using density functional theory (DFT). The calculated B3LYP//RHF bond lengths are slightly longer than those of LSDA//RHF. The calculated electronic structures clearly show that TPP and meso-catecholic group contribute to π-electron conjugation along porphyrin ring for HOMO and LUMO, significantly reduced the HOMO-LUMO gap. The wavelength due to B3LYP energy gaps is favored with experimental value in Soret (B), and LSDA energy gaps are favored with experimental value in visible bands (Q). The electronic effect of the catecholic groups is to reduced energies of both the HOMOs and LUMOs. However, the distortion of porphyrin predominantly raises the energies of the HOMOs, so the net result is a large drop in HOMO and smaller drop in LUMO energies upon meso-substituted catecholic group of the porphyrin macrocycle as shown in KP99151 and KP99152 of Figure 5(a). These results are in reasonable agreement with normal-coordinate structural decomposition (NSD) results. The HOMO-LUMO gap is an important factor to consider in the development of photodynamic therapy (PDT).

• Journal of Microbiology and Biotechnology
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• 제30권2호
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• pp.271-278
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• 2020

Glycerol dehydrogenase (GlyDH) catalyzes the oxidation of glycerol to dihydroxyacetone (DHA), which is the first step in the glycerol metabolism pathway. GlyDH has attracted great interest for its potential industrial applications, since DHA is a precursor for the synthesis of many commercially valuable chemicals and various drugs. In this study, GlyDH from Klebsiella pneumoniae (KpGlyDH) was overexpressed in E. coli and purified to homogeneity for biochemical and molecular characterization. KpGlyDH exhibits an exclusive preference for NAD⁺ over NADP⁺. The enzymatic activity of KpGlyDH is maximal at pH 8.6 and pH 10.0. Of the three common polyol substrates, KpGlyDH showed the highest k_cat/K_m value for glycerol, which is three times higher than for racemic 2,3-butanediol and 32 times higher than for ethylene glycol. The k_cat value for glycerol oxidation is notably high at 87.1 ± 11.3 sec^-1. KpGlyDH was shown to exist in an equilibrium between two different oligomeric states, octamer and hexadecamer, by size-exclusion chromatography analysis. KpGlyDH is structurally thermostable, with a T_m of 83.4℃, in thermal denaturation experiment using circular dichroism spectroscopy. The biochemical and biophysical characteristics of KpGlyDH revealed in this study should provide the basis for future research on its glycerol metabolism and possible use in industrial applications.

• 펄프종이기술
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• 제46권1호
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• pp.1-10
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• 2014

Xylanase (X) derived from Aurreobasidium pullulans and laccase-mediator system (LM) using Trichophyton sp. LKY-7 laccase (TrL) and N-hydroxy-2-pyridone analogue (NHP) as a mediator were applied in hardwood kraft pulp (HwKP) bleaching. The individual and the synergistic effects of X and LM stage were investigated in the enzymatic bleaching of HwKP. Also, the effects of subsequent alkaline extraction (E) and alkaline/hydrogen peroxide treatment (P) were examined. In X or LM treatment alone, an appreciable bleaching effect of HwKP was not observed, whereas subsequent E or P stage enhanced the increase of brightness and the decrease of kappa number. Especially, P stage significantly enhanced the bleaching effect of pulp. Bleaching of HwKP with XLM sequentially gave significantly higher pulp brightness and lower kappa number than that obtained after the treatment of HwKP with X+LM simultaneously. When HwKP was sequentially treated with XLM followed by P stage, the brightness increased by about 11% ISO and the kappa number decreased by about 3.6 in comparison with the initial pulp. Xylanase and laccase were strongly inactivated by NHP both in the absence and the presence of pulp.

• 한국식품영양학회지
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• 제20권4호
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• pp.378-383
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• 2007

To study the characteristics and processing of Kochujang which is rapidly fermented by commercial enzymes, three kinds of Kochujang(KP-FA, KP-FN, and KP-BN) using commercial proteases and one Kochujang(KM) using Meju were prepared and their qualities investigated. There were only small differences in pH and acidity between each Kochujang. The moisture contents were high tendency in the three kinds of Kochujangs using the commercial proteases at 20 days of fermentation. Reducing sugars had a tendency to decrease during the fermentation in the Kochujangs using the proteases. During the first half of fermentation, the Kochujangs made with proteases showed higher amino nitrogen contents than the Kochujang(KM) made using Meju. Acidic protease activity was high in KP-FA at 20 days of fermentation and neutral protease activity was high in KP-FN and KP-BN at the beginning of fermentation. The Kochujangs made using the proteases, through 20 days of fermentation, obtained high preference in the sensory evaluation for color, texture, and overall acceptability. However, the hot taste was stronger in these Kochujangs during the fermentation.