• Mycobiology
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• 제37권2호
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• pp.147-151
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• 2009

The wood-rotting fungi of three Korean islands in the Yellow Sea, Soyeonpyung-do (SY), Oeyeon-do (OY), and Gageo-do (GG), were investigated from October 2004 to November 2005. During four sampling trips on each island, a total of 46, 44, and 44 specimens were collected from SY, OY, and GG, respectively. The identified wood-rotting fungi from SY included 29 species of 22 genera and nine families; OY had 31 species of 26 genera and 10 families; and GG had 34 species of 27 genera and 11 families. The majority of the fungi were in the Polyporaceae, which was represented by 23 genera and 30 species. Auricularia polytricha, Daedaleopsis tricolor, Daldinia concentrica, Hymenochaete cinnamomea, Hymenochaete yasudai, Hyphoderma setigerum, Lopharia mirabilis, Schizopora paradoxa, and Trametes versicolor were collected from all three islands.

• Journal of the Korean Wood Science and Technology
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• 제23권4호
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• pp.108-116
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• 1995

Guaiacol을 첨가한 potato-dextrose agar배지와 참나무 목분 배지를 이용하여 배지의 발색 정도를 정량함으로써 리그닌 분해와 리그닌 분해효소 생산을 위한 우수 균주분리를 시도하였다. 배지의 발색정도와 리그닌 분해율과는 정의 상관을 나타내어 미지균의 리그닌 분해력 추정을 가능하게 하였으며, 버섯의 자실체와 부후재로 부터 분리한 리그닌분해균 중에서 리그닌 분해력과 laccase활성이 우수한 LKY-12, LKY-7과 Coriolus versicolor-13 균주를 선발하였다. 이들 균주의 리그닌 분해율은 30~35% 범위이고, glucose-peptone broth에서 리그닌 분해효소 활성이 다른 균주에 비하여 매우 높아서 우수균주의 특성을 나타냈으며, 생물학적인 펄프화 및 표백 그리고 리그닌분해효소 생산에 이용 가능한 균주로 생각되었다.

• Journal of the Korean Wood Science and Technology
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• 제27권4호
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• pp.72-79
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• 1999

The ability of several wood rotting fungi for decolorization of two anthracene derivatives, Carminic acid (CA) and Remazol brilliant blue R (RBBR), and hardwood KP bleaching liquor (BL) as well as laccase activities in these fungi were studied. The enzyme activity appeared exclusively in fungi destaining RBBR and CA, but in the case of BL, such relationship was not observed. The laccase enzyme was released into the decolorization media and its inducible (but not constitutive) forms shown destaining activity. The purified inducible forms of Kuehneromyces mutabilis and Pleurotus ostreatus laccase destained CA. Thus the possible differentiation between specificity of particular LAC forms was confirmed. In addition the nitrogen starvation induced both laccase and CA destaining activities, but the increase was higher for decolorization of CA than LAC activity. Probably LAC would be only partly responsible for decolorization of this dye. This results suggested that purified LACs decolorize CA, however its destaining activities were considerably lower than the activities on syringaldazine.

• 한국미생물·생명공학회지
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• 제31권3호
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• pp.301-306
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• 2003

Twenty-one different fungi were tested for their ability to decolorize a wide range of structurally different dyes. Twenty fungal strains were isolated from fruiting bodies which were collected at the Kwangneung National Arboretum, Korea. One fungal strain were isolated from a rotting wood at Soongsil University, Korea. Nine kinds of dyes were used: three anthraquinone dyes and six azo dyes. The five fungal strains, Laetiporus sulphureus, Polyporus arcularius. Auricularia polytricha, Stereum ostrea, and Bjerkandera sp. UK-l showed decolorization ability. Except Auricularia polytricha, the four fungal strains were wood rotting fungi, and belonged to Aphyllophorales. Bjerkandera sp. UK-I, which was a white rot fungus, could decolorize all kinds of dyes tested in this study, indicating this fungus is one of candidates for applying in biological methods of dye waste treatment.

• 한국균학회지
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• 제21권1호
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• pp.51-63
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• 1993

1990년 3월부터 1992년 2월까지 우리나라의 국립공원지대(國立公園地帶)와 일부지역(一部地域)과 일부도서(一部島嶼)를 중심으로 목재부후균류(木材腐朽菌類)를 탐색하여 자낭균류(子?菌類)와 목이류(木耳類)의 균류(菌類)들을 동정하고 기존의 해당 목재부후균류(木材腐朽菌類)와 함께 분류한 결과 52 종(種), 1 아종(亞種), 및 1 품종(品種)으로 집계되었으며 그중 2 속(屬) 3 종(種)은 국내 미기록(未記錄)으로 밝혀졌다. 본 연구를 통하여 방석꼬투리 버섯속(屬) (신칭(新稱), Hypoxylon) 과 주발목이속(木耳屬) (신칭(新稱), Femsjonia)이 미기록속(未記錄屬)으로, 큰방석꼬투리버섯 (신칭(新稱), Hypoxylon punctulatum), 분홍좀목이 (신칭(新稱), Exidia recisa), 그리고 노란주발목이 (신칭(新稱), Femsjonia pezizaeformis)가 미기록종(未記錄種)으로 확인되어 한국산(韓國産) 균류목록(菌類目錄)에 새로이 추가 되었다.

• Mycobiology
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• 제33권4호
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• pp.167-172
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• 2005

Through ten field surveys in Kanghwa Island from August of 1997 to March of 2002, total 107 specimens of wood-rotting fungi belonging to the Aphyllophorales were collected and identified to the species. They taxonomically amounted to 10 families, 31 genera, and 48 species. Among them, one family, Steccherinaceae, and four genera, Australohydnum, Castanoporus, Dacryobolus and Haplotrichum were confirmed as new to Korea. Five unrecorded species, Australohydnum dregeanum, Castanoporus castaneus, Dacryobolus karstenii, Haplotrichum conspersum and Hyphoderma odontiiforme were registered here with descriptions.

• 한국균학회지
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• 제10권4호
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• pp.203-206
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• 1982

1981년(年) 9월(月)부터 1982년(年) 10월(月)까지 서울대학교(大學校) 수원(水原)캠퍼스내(內)에서 목재부후균(木材腐朽菌)을 채집(採集)하였다. 이들은 2목(目), 8과(科), 22속(屬), 34종(種)으로 분류(分類)되였으며, 그 중(中) 한국산(韓國産) 미기록속(未記錄屬) Gloeoporus와 미기록종(未記錄種) G. adustus(Willd. ex Fr.) $Pil\acute{a}t$ 및 G. dichrous(Fr.) Bres.가 발견(發見)되었다. 이들 신종(新種)에 대해 우리말 버섯이름이 주어졌으며, 자실체(子實體)에 대한 기술(記述)을 덧붙였다. 채집(採集)된 34종(種)에 대해 각각(各各) 서식(棲息)하는 나무의 속명(屬名)을 기재(記載)하였다. 각(各) 표본(標本)은 서울대학교(大學校) 농과대학(農科大學) 농생물학과(農生物學科)에 보존(保存)되어 있다. 한국산(韓國産) 미기록종(未記錄種)의 동정(同定)에 협조(協助)하여 주신 미국(美國) Virginia주립대학교(州立大學校) Orson K. Miller, Jr. 교수(敎授)에게 감사(感謝)를 드린다.

• Journal of the Korean Wood Science and Technology
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• 제47권2호
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• pp.210-220
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• 2019

This work aimed to evaluate the influence of culture conditions on laccase production in the co-culture of wood-rotting fungus with Trichoderma sp. The effects of infection extent, infection time, and culture filtrate of Trichoderma sp. on the laccase production by wood-rotting fungus in co-culture were examined. T. rubrum LKY-7 and T. longibrachiatum were selected as fungi which are effective in co-culture for laccase production. A significant increase in laccase activity was observed when T. rubrum LKY-7 was co-cultured with T. longibrachiatum in glucose-peptone liquid medium, yielding an increase of more than 5 times in laccase activity, as compared with control. Laccase production by T. rubrum LKY-7 during co-culturing was significantly influenced by the infection extent and the infection time of T. longibrachiatum. Maximal laccase activity was obtained when T. rubrum LKY-7 culture was infected by T. longibrachiatum after 3 days of cultivation at an inoculum size ratio of 0.5 to 1. The addition of culture filtrate or autoclaved mycelium of T. longibrachiatum to T. rubrum LKY-7 culture did not significantly enhance laccase production by T. rubrum LKY-7 as compared with control (mono cultures of T. rubrum LKY-7).

• Journal of the Korean Wood Science and Technology
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• 제44권2호
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• pp.253-263
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• 2016

Fungi, such as the wood-rotting Polyporus brumalis, are excellent sources of pharmaceutically interesting natural products such as sesquiterpenoids. In this study, we investigated the biosynthesis of P. brumalis sesquiterpenoids on modified medium. Ten additional species of white rot fungi were inoculated in medium containing nutrients such as $C_6H_{12}O_6$, $C_4H_{12}N_2O_6$, $KH_2PO_4$, $MgSO_4$, and $CaCl_2$ at $28^{\circ}C$ for 25 days. After 10 days of incubation, eudesmane-type sesquiterpenes, ${\beta}$-eudesmane and ${\beta}$-eudesmol, were only synthesized during the growth phase of P. brumalis. Experiments excluding one nutrient at a time were conducted to determine the effects of inorganic nutrients on sesquiterpene biosynthesis. In conclusion, GC-MS analysis showed that biosynthesis of sesquiterpenes was differentially regulated by inorganic nutrients such as $MgSO_4$, $C_4H_{12}N_2O_6$, and $KH_2PO_4$. We found $MgSO_4$ supplementation to be vital for eudesmane-type sesquiterpene biosynthesis in P. brumalis; nitrogen ($C_4H_{12}N_2O_6$) and phosphate ($KH_2PO_4$) inhibited the synthesis of P. brumalis metabolites. Magnesium is a known cofactor of sesquiterpene synthase, which promotes ${\beta}$-eudesmol synthesis. To mechanistically understand eudesmane-type sesquiterpene biosynthesis in P. brumalis, further research into the genes regulating the dynamics of such biosynthesis is warranted.

• Journal of the Korean Wood Science and Technology
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• 제26권3호
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• pp.41-47
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• 1998

Some white-rot fungi, screened at the Laboratory of Forest Products Microbiological Chemistry, Chungbuk National University were cultured and added the inducer of laccase enzyme, 2,5-xylidine. The fungi named by CB-13, CB-20, CB-99, CB-100 and CB-123 strains showed positive results in the decolorization of aromatic compounds, carminic acid and Rhemazol brilliant blue R. Concerned to the inducing effect of 2,5-xylidine on laccase activity, CB-20, CB-100 and CB-123 strains showed very high activity by addition of 2,5-xylidine, whilst CB-13, CB-99 and CB-124 strains produced relatively high laccase enzymes, regardless of inducer addition. There were no any laccase activities on CB-25, CB-64 and CB-139, even in addition of inducer. It is confirmed that some screened fungi have decolorizing ability on aromatic compounds, carminic acid and Rhemazol brilliant blue R. Also, the addition of inducer, 2,5-xylidine, has increased the activity of laccase enzyme which is secreted from some white-rot fungi.