• 한국산림과학회지
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• 제87권4호
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• pp.543-548
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• 1998

소나무와 해송으로부터 엽록체상의 두 유전자 psbD와 rbcL를 PCR에 의해 증폭한 후 제한효소 HaeIII를 사용해서 절단했다. 두 개의 종 특이적 엽록체 DNA 단편이 확인되었고, 이 두 개의 표지자를 이용하여 소나무(충북3호)와 해송(남난37호)의 인공교잡 가계로부터 엽록체 DNA의 부계 유전양식이 확인되었다. 인공교잡 가계에 있어서 엽록체 DNA의 부계 유전양식을 근거로 해송으로부터 소나무로의 이입교잡에 의해 생겨났다는 가설(현신규 등, 1967)이 지배적인 금강송 115개체로부터 이입교잡에 의해 유입되어진 흔적을 구명하기 위하여 해송 특이 엽록체 DNA의 존재 여부를 검색하였다. 분석에 사용된 금강송 전 개체에서 소나무에서 관찰된 엽록체 DNA(psbD와 rbeL)의 절편 분획 양상과 동일한 절편 분획 양상이 확인되었다. 본 실험의 결과로부터는, 금강송에는 해송으로부터 유입된 엽록체 게놈의 흔적을 찾아 볼 수 없었으며, 따라서 금강송을 소나무(♀)약 해송(♂)의 이입교잡종이이라고 간주할만한 확고한 증거를 제시할 수 없었다.

• Journal of the Korean Wood Science and Technology
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• 제34권5호
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• pp.1-10
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• 2006

The objective of this study was to elucidate the microscopic patterns of decay caused by brown-rot fungi of Tyromyces palustri and Gloeophyllum trabeum in Korean red pine (Pinus densiflora) and radiata pine (Pinus radiata) woods through light and electron microscopies. The ultrastructural changes of cell walls attacked by the two brown-rot fungi were compared in this respect. Macroscopically, radiata pine showed more ring and radial checks than Korean red pine. Microscopically, with the progress of decay, spiral checks associated with cross-field pits and bore holes in the cell wall were more remarkably numerous in the radiata pine than in the Korean red pine. In the radiata pine, G. trabeum produced more spiral checks in the cell wall than T. palustris. In the advanced stages of decay by G. trabeum, the erosions of ray cell walls were identified both in the Korean red pine and radiata pine but $S_3$ layers of tracheid walls were eroded only in the Korean red pine.

• Journal of the Korean Wood Science and Technology
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• 제39권3호
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• pp.244-251
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• 2011

본 연구에서는 소나무(Pinus Densiflora)와 잣나무(Pinus Koraensis)에 대한 연소특성을 비교 평가하였다. 두 수종은 한옥의 부재로 널리 사용되고 있으며, 그 체적밀도는 소나무가 잣나무에 비하여 상대적으로 큰 값을 가지고 있다. 목재의 연소특성은 해당 수종의 체적밀도와 밀접한 관계를 갖고 있는데 본 연구에서 방염성능에 있어서는 뚜렷한 차이를 확인하기는 어려웠지만 콘칼로리미터 시험방법에 있어서는 총 방출열량, 평균 열방출률 비교를 통하여 목재 수종의 체적밀도의 상관성을 확인하였다. CO/$CO_2$의 비로부터 알 수 있는 연소가스의 유해성은 난연처리에 의해 높아진 것으로 나타났다.

• Journal of the Korean Wood Science and Technology
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• 제42권1호
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• pp.58-67
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• 2014

울진산 금강송의 재질특성 조사의 일환으로 기계적 성질을 조사하였다. 종압축강도는 $36.2N/mm^2$로 국산 소나무재와 잣나무재보다 다소 낮은 값을 나타내었다. 수로부터의 거리에 따른 종압축강도는 수에서 60 mm 떨어진 지점에서 최대값을 나타내었다. 휨강도는 $76.5N/mm^2$로 나타났으며, 국산 소나무재와 잣나무재보다 다소 높은 값을 나타내었다. 그리고 수로부터 60 mm 떨어진 지점에서 최대값을 나타내었다. 전단강도는 높은 비중을 나타내었던 수 부근에서 $10.4N/mm^2$로 최대값을 나타내었으며, 80 mm 지점에서 $9.1N/mm^2$로 최소값을 나타내었다. 표면경도는 횡단면이 $43.7N/mm^2$, 방사단면이 $12.0N/mm^2$, 접선단면이 $13.7N/mm^2$으로 나타났다. 이러한 결과는 비슷한 비중을 나타낸 소나무의 표면경도 값과 유사하다. 금강송의 기계적 성질은 비슷한 비중을 가지는 소나무과 수종인 소나무 및 잣나무와 비슷하였다. 그리고 금강송의 기계적 성질은 평균연륜폭보다 비중에 의해 더욱 큰 영향을 받는다는 것을 확인할 수 있었다.

• The Korean Journal of Ecology
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• 제19권1호
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• pp.9-19
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• 1996

The model PINUSCO2 hased of physiology was creted to simulate carbon dioxide budget in a population of red pine(pinus densiflora) which is one of the dominant species in Korea. Driving forces of PINUSCO2 are global radiation, maximum and minimum air temperatures. State variables fo the model are standing crops of leaf, branch, trunk and root of the red pine population. PINUSCO2 calculates net photosynthesis of canopy and respiration of each organ with 1 hour time step. PINUSCO2 estimated the annual gross productivity, respiration and net productivity of the red pine population as 43.99, 24.55, and 19.44 ton CO2·ha-1·yr-1, respectively, at the study sity(35°58′00"N, 128°25′35"E). PINUSCO2 showed that the red pine population grew mainly in spring and fall, and that in summer daily net population productivity frequently became negative.

• Mycobiology
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• 제29권4호
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• pp.205-209
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• 2001

The Korean sawmills have recently recognized the importance of prevention of fungal discoloration due to increased losses in revenue. Before establishing integrated control strategies of fungal discoloration, more complete knowledge about causal organisms is needed. As a first step, we initiated a through survey of fungi colonizing commercially important softwood(Pinus dens flora, Pinus koraiensis, and Pinus radiata) logs and lumber in Korea. In this paper we report results obtained from Japanese red pine(Pinus densiflora) log study. In summer 2000, fungi were isolated from Japanese red pine logs in storage, and identified based on their cultural and morphological characteristics. A total of 595 fungi were isolated, representing 21 genera and 30 species. Mold fungi, mostly Trichoderma species, were the most frequently isolating fungi, representing more than half of all isolates. Dematiaceous fungi represented approximately one fifth of the isolates, and Rhinocladiella atorvirens was the most abundant in all samples. Opiostoma species represented 7% of all isolates from cores planted on malt extract agar(MEA) and the incidence of these species doubled with the addition of streptomycin and cycloheximide to MEA. The results indicate that Japanese red pine sapwood is susceptible to colonization by a variety of fungal species. As a result, control strategies that concentrate on one fungus may have limited success because of interference from competing flora.

• Journal of the Korean Wood Science and Technology
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• 제47권4호
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• pp.498-508
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• 2019

This study was carried out to investigate the potential of Korean red pine (Pinus densiflora) inner bark extracts as an antidiabetic agent. The ethyl acetate soluble fraction of the bark extracts was chromatographed on a Sephadex LH-20 column to yield five compounds, which structures were elucidated by NMR spectroscopy. The isolated compounds were (+)-catehin, (-)-epicatechin, taxifolin, taxifolin-3'-O-${\beta}$-D-(+)-glucose and $\tilde{n}$-courmaric acid. The antidiabetic activity of the different fractions, including the crude extracts and isolated compounds, was evaluated by ${\beta}$-cells insulin secretion and glucose uptake in skeletal muscle cells. The insulin secretion was 128% for taxifolin at $25{\mu}g/mL$. However, the other samples had no effect on this test. For the glucose uptake activity assay, $1{\mu}M$ insulin and 2 mM metformin were used as controls. Both the crude extract and taxifolin showed relatively low activity values, but the other samples yielded glucose uptake values over 260%. ${\rho}$-courmaric acid showed the highest uptake (270%). The results confirmed that Korean red pine extracts may be used as a hypoglycemic agent.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.119-119
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• 2019

Pinus densiflora, the Korean Red Pine, is the predominant tree species of the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. Pinus densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones contain many bioactive phytochemicals, but they are rarely used as natural raw materials. This study was conducted to evaluated the anti-inflammatory effect of pine cone extracts and its possbility of natural sources were evaluated. Pine cones were extracted with 80% methanol, concentrated and then partitioned with ethyl acetate, and the organic layer was used as a sample. The Pine cone Ethyl acetate Fraction (PEF) showed no toxicity to RAW 264.7 cells at a concentration of less than $50{\mu}g/ml$. PEF inhibited the production of nitric oxide (NO) in RAW 264.7 cells treated with lipopolysaccharide (LPS). Also, It suppressed the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and transcription of nuclear factor-kappa B (NF-${\kappa}B$). These results suggest that pine cones can be used as an effective natural material for anti-inflammatory agent.

• 한국자원식물학회지
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• 제32권4호
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• pp.275-281
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• 2019

The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than $100{\mu}g/mL$. PEF inhibited the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.

• Journal of the Korean Wood Science and Technology
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• 제15권2호
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• pp.53-66
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• 1987

Korean red pine (Pinus densiflora S. et. Z.) and pitch pine(Pinus rigida Mill) $5{\times}10cm$ dimension lumber were dried in a kiln providing a cross-circulation velocity of 5 m/sec at dry-and wet-bulb temperatures of 116 and $71^{\circ}C$, followed by 3 hours at 91 and $85^{\circ}C$. Compared to dimension lumber dried lumber were as follows. 1. To dry to 10 percent moisture content, the high-temperatures schedule of Korean red pine and pitch pine lumber took less than one seventh the time required by the conventional kiln drying schedule. 2. High-temperature drying rate and conventional drying rate to 10 percent moisture content of Korean red pine lumber were 2.75 and 0.35%/hr, and those of pitch pine lumber were 3.38 and 0.46%/hr respectively. 3. Compared to lumber of both species on conventional schedule, moisture gradient of high-temperature lumber was greater. 4. Compared to lumber on conventional schedule, maximum surface checking of high-temperature lumber of both species was severer, and maximum end checking of high-temperature lumber of both species was similar to that of lumber on conventional schedule. 5. Compard to lumber on conventional schedule, Korean red pine lumber dried at high temperature showed more honeycombing, but pitch pine lumber dried at high-temperature showed significantly slighter honeycombing. 6. Compared to lumber on conventional schedule, the high-temperature lumber showed less warping lumber of both species. 7. Collapse and casehardening of Korean red pine and pitch pine lumber on both scheules were slight.