• Journal of the Korean Society of Tobacco Science
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• v.25 no.1
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• pp.12-19
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• 2003

Transgenic tobacco plants were selected by using the transformation of putrescine N-methyltransferase(PMT) gene, the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine. PMT was fused in reverse orientation to the CaMV 35S promoter of the plant expression vector pBTEX(pPAB3) to produce tobacco plants of low nicotine content. To compare nicotine content, only pBTEX vector and PMT gene which was fused in forward orientation to the CaMV 35S promoter(pPAB2) were also transformed to the leaf tobacco plants(Nicotiana tabacum cv. NC82 and N. tabacum cv. Br2l). The presence of sense- and antisense-PMT gene, and pBTEX vector in the transgenic plant was confirmed by genomic PCR.

• Korean Journal of Clinical Pharmacy
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• v.9 no.2
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• pp.103-108
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• 1999

The anti-proliferating effects of some plants on hepatoma cell lines were studied by the 3-[4,5-dim-ethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide (MTT assay), to investigate the anticancer effect with some plants around here. As the result, we saw that the anti-prolferating effect to the plants. Among the plants, Equisetum arvense L. and Lactuca dentata Makino. var, flaviflora Makino of them relatively showed a good ant-proliferating effect. Capsicum annuum L. var. angulosum Mill (Leaf) was the best among them. We also examined morphological changes on the hepatoma cells in this process. In case of Capxicum annuum L. var. angulosum Mill, the tells become vague after 2 days, and then destroyed faster than others. We can fee also the condensated chromosome on the treated cells with Capxicum annuum L. var. angulosum Mill. And we also observed condensation through using a fluorescent microscope by PI staining, and observed DNA fragmentation.

• Korean journal of applied entomology
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• v.31 no.2
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• pp.139-143
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• 1992

Feeding plants of rice water weevil(RWW) adult and larvae were observed. Thirteen families including 104 species were found as host plants of RWW adult, and 74 species of them were newly identified as its host. Thmedia triandra var. japonica, Luzula capitata, Agropyron tsukushiense var. ransiens, and Zoysiama crostachya were most favorable hosts of adult RWW. Host plants for larvae were 9 species of weeds including Cperus nipponicus, Eleccharis kuroguwa, Sagittaria trifolia, and Claviceps purpurea in the rice paddy fields.

• Korean Journal of Plant Resources
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• v.23 no.3
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• pp.256-260
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• 2010

We performed the floristic study of Carpathian mountains in Ukraine from April 2008 till October 2009. We designated eight important areas to investigate in Ukraine Carpathians including Svydovets, Chornohora, Kuziy-Trybushany, Maramorosh, Uholka-Shyrokyi Luh, Narcissi Valley, Chorna Hora, and Yulivska Hora. The total numbers of vascular plants in investigated area were 1,349 speices from 502 genera and 112 families. The endemic species to Carpathians numbered to 65 from 49 genera belonging to 24 families, and it was corresponded to 4.8% of the 1,349 vascular species. We prepared the list of vascular plants with compiling database on useful plants. Ukraine Carpathians endemic species could be applied as high value products in research and practice. Moreover, the flora of Carpathian mountains which is distributed in five nations in eastern Europe and famous for Primeval Beech Forests (Fagus sylvatica), is useful to compare the floristic study with Korean flora.

• Journal of the Korean Society of Combustion
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• v.18 no.3
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• pp.1-8
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• 2013

Thermodynamic and economic analyses of various types of gas turbine combined cycle power plants have been performed to establish criteria for optimization of power plants. The concept of efficiency, in terms of the difference in energy levels of electricity and heat, was introduced. The efficiency of power and heat generation by power plants with other purposes was estimated, and power generation costs were figured out for various types of combined heat and power plants(i.e., fired and unfired, condensing and non-condensing modes, single or double pressure HRSG).

• Korean Journal of Pharmacognosy
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• v.13 no.1
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• pp.14-19
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• 1982

Potential toxicity of 15 medicinal plants used for herbal drugs, which were also described as being tonic for hematopoietic system or being toxic for the system in a oriental book 'Dong Ee Bo Gam', were evaluated in mice. Six plants among 15 plants tested appeared to exhibit acute toxicity along with bone marrow depression or with abnormally enhancing the $^3H-thymidine$ incorporation into DNA biosynthesis in bone marrow cells. Six plants were Paeonia albiflora, Pharbitis nil, Cemphalia lapidescens, Scutellaria baicalensis, Akebia quinata and Glycyrriza uralensis.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.555-560
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• 2021

During disease surveys conducted from 2019 to 2021 in Korea, leaf spot symptoms in rhubarb (Rheum rhabarbarum) plants were frequently observed in plants growing in fields located in Cheolwon, Taebaek, and Inje in Gangwon Province. The incidence of diseased leaves of the plants in the fields ranged from 2% to 80%. Nine single-spore isolates of Phoma sp. were obtained from lesions of the diseased leaves; all of them were identified as Didymella rhei based on their cultural, morphological, and molecular characteristics. Three isolates of D. rhei were tested for pathogenicity on rhubarb leaves using artificial inoculation. All the tested isolates caused leaf spot symptoms in the inoculated plants; the symptoms were similar to those observed in plants from the fields investigated. This is the first report of D. rhei causing leaf spot in rhubarb in Korea.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.519-521
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• 2020

Damping-off symptoms were frequently observed on young plants of Indian lettuce (Lactuca indica) grown in a farmer's vinyl greenhouse located in Goseong, Gangwon province of Korea during a disease survey in June 2019. The incidence of diseased plants in the vinyl greenhouse investigated was 30-50%. Four isolates of Rhizoctonia sp. were obtained from the diseased plants and identified as Rhizoctonia solani AG-4 based on the morphological characteristics and anastomosis test. Three isolates of R. solani AG-4 were tested for pathogenicity on Indian lettuce by artificial inoculation. All the tested isolates induced damping-off symptoms on the inoculated plants. The symptoms were similar to those observed in plants from the farmer's vinyl greenhouse investigated. This is the first report of R. solani AG-4 causing damping-off in Indian lettuce.

• The Korean Journal of Mycology
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• v.49 no.2
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• pp.249-251
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• 2021

Damping-off symptoms were frequently observed in young deltoid synurus (Synurus deltoides) plants grown in a farmer's vinyl greenhouse located in Hwacheon, Gangwon Province, Korea, during a disease survey in July 2019. The incidence of diseased plants in the vinyl greenhouse investigated was 5-30%. Five isolates of Rhizoctonia sp. were obtained from the diseased plants and identified as Rhizoctonia solani AG-1 (IB), based on morphological and cultural characteristics and anastomosis test results. Three isolates of R. solani AG-1 (IB) were tested for pathogenicity on deltoid synurus using artificial inoculation. All the tested isolates induced damping-off symptoms in the inoculated plants. The symptoms were similar to those observed in plants from the farmer's vinyl greenhouse. To our best knowledge, this is the first report of R. solani AG-1 (IB) causing damping-off in deltoid synurus.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.148.1-148
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• 2003

Natural virus infection of cultivated Daphe odora plants showing chlorosis and stunting was observed and their causal agent was investigated. An isolate of isometic virus was purified from infected leaf tissues, and it could infect systemic severe mosaic on Chenopodium quinoa and C. amaranticolor. cDNA library was generated from partially purified viral RNAs and oligo dT primer-pSPORTl system, and recombinant clones were selected and their inserts were sequenced randomly. Nucleotide sequences of the virus were analyzed by BLAST, and it was closely related to members of subgroup B in the genus Nepovirus. The sequence analysis suggest that the virus was identified as an isolate of Cycas necrotic stunt virus (CNSV) because it was 89.7 ％ and 94.7 ％ identical to known CNSV for the CP and 3' noncoding region, respecitively. RT-PCR was performed to screen disease incidence of CNSV in Daphe plants, and five out of 10 plants (50 ％) were infected by CNSV This is the first sequence information of CNSV from Daphe plants.