• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.3
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• pp.8-18
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• 1998

This study was to make a effective seed mixture types with Korean native plants by researching te seed timing of suitable woody plants and competitive germination results of various plants for the purpose of cut-slotes revegetation. The results are summarized as follows. 1. In field polystand experiment, comparative among severalcomposition combination for early coverage shows good condition. 2. In combination for early-coverage, mixture of native woody plants only seeded in May and June shows over 90% coverage. It was not inferior to the other mixture types. So mixture of Albizzia Julibrissin, Cymbopogon doeringii, Alnus hirsuta and Indigfera pseudo-tinctoria is able to use to mixture type. 3.In case of combination for scenic beauty, Rhus chinensis shows low germination in the mixture for scenic beauty. But 90 days after seeded in May, it recorded maximum 44 cm height, seeded in June recorede 18 cm height. 4. Lespedeza crytobotrya shows good elongation in the combination for early coverage and scenic beauty, but that effects on elongation of other plants. So, it demands close investigation about suitable mixing rate of plants. 5. Alnus hirsuta, Pinus thunbergii, Betula platyphylla, Spiraea prunifolia var. simpliciflora have been rarely germinated. So, the mixture of these plants wasn't adequate to use for revegatation.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.143-157
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• 2019

Medicinal plants are high-value natural resources that have been used as precautionary drugs by many people globally. The increasing global demand for bioactive compounds from medicinal plants has led to the overexploitation of many valuable species. One widely used approach to overcome this problem is the use of adventitious root cultures as a propagation strategy. This review examines the scientific research published globally on the application of adventitious root cultures for many medicinal plants. Adventitious roots generated under aseptic environments in suitable phytohormone-augmented medium exhibit high growth rates and production of important secondary metabolites. Parameters such as medium properties and composition, growth hormone type, and elicitation strategies for in vitro grown adventitious roots of medicinal plants, are the main topics discussed in this review. We also examine current developments in bioreactor system cultivation for plant bioactive compounds using adventitious root cultures, a technology with possible commercial applications, via several studies on adventitious root culture of medicinal plants in which bioreactor systems play a role. In conclusion, the development of adventitious root cultures for medicinal plants is highly useful because of their capability for vegetative propagation and germplasm preservation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.2
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• pp.31-48
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• 2019

The purpose of this study is to present the basic data for establishing the plan of usage and management of healing forest by surveying and analysing the vascular plants distributed in Jangseong Healing Forest. The numbers of vascular plants were summarized as 338 taxa including 85 families, 211 genera, 294 species, 3 subspecies, 33 varieties and 8 forms. In the result of life form, hemicryptophytes and geophytes were the most common and hydrophytes were the least. The rare plants were 5 taxa including Abies koreana Wilson(planted), Thuja orientalis L.(planted), Scutellaria insignis Nakai, Tricyrtis macropoda Miq. and Arisaema heterophyllum Blume. The Korean endemic plants were 12 taxa including Philadelphus schrenkii Rupr., Indigofera koreana Ohwi, Lycoris flavescens M.Y.Kim ＆ S.T.Lee and so forth. The specific plants by floristic region were 28 taxa including 2 taxa of grade IV, 3 taxa of grade III, 7 taxa of grade II and 16 taxa of grade I. The naturalized plants were 20 taxa including Fallopia convolvulus (L.) $A.L{\ddot{o}}ve$, Ailanthus altissima (Mill.) Swingle, Veronica arvensis L., Poa pratensis L. and so forth. The invasive alien plants were Rumex acetocella L. and Ambrosia artemisiifolia L..

• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.407-415
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• 2012

An endophytic bacterium, Bacillus thuringiensis GS1, was isolated from bracken (Pteridium aquilinum) and found to have maximal production of chitinase (4.3 units/ml) at 5 days after culture. This study investigated the ability of B. thuringiensis GS1 to induce resistance to Rhizoctonia solani KACC 40111 (RS) in cucumber plants. Chitinase activity was greatest in RS-treated plants at 4 days. ${\beta}$-1,3-Glucanase activity was highest in GS1-treated plants at 5 days. Guaiacol peroxidase (GPOD) activity increased continuously in all treated plants for 5 days. Ascorbate peroxidase (APX) activity in RS-treated plants was increased 1.5-fold compared with the control at 4 days. Polyphenol oxidase (PPO) activity in RS-treated plants was increased 1.5-fold compared with the control at 3 days. At 5 days after treatment, activity staining revealed three bands with chitinase activity (Ch1, Ch2, and Ch3) on SDS-PAGE of cucumber plants treated with GS1+RS, whereas only one band was observed for RS-treated plants (Ch2). One GPOD isozyme (Gp1) was also observed in response to treatment with RS and GS1+RS at 4 days. One APX band (Ap2) was present on the native-PAGE gel of the control, and GS1- and GS1+RS-treated plants at 1 day. PPO bands (Po1 and Po2) from RS- and GS1+RS-treated plants were stronger than in the control and GS1-treated plants upon native-PAGE at 5 days. Taken together, these results indicate that the induction of PR proteins and defense-related enzymes by B. thuringiensis GS1 might have suppressed the damping-off caused by R. solani KACC 40111 in cucumber plants.

• The Plant Pathology Journal
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• v.39 no.2
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• pp.207-219
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• 2023

Soft rot is a widespread, catastrophic disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) that severely damages the production of Amorphophallus spp. This study evaluated the rhizosphere bacterial and fungal communities in Pcc-infected and uninfected plants of two species of Amorphophallus, A. muelleri and A. konjac. Principal component analysis showed that the samples formed different clusters according to the Pcc infection status, indicating that Pcc infection can cause a large number of changes in the bacterial and fungal communities in the Amorphophallus spp. rhizosphere soil. However, the response mechanisms of A. muelleri and A. konjac are different. There was little difference in the overall microbial species composition among the four treatments, but the relative abundances of core microbiome members were significantly different. The relative abundances of Actinobacteria, Chloroflexi, Acidobacteria, Firmicutes, Bacillus, and Lysobacter were lower in infected A. konjac plants than in healthy plants; in contrast, those of infected A. muelleri plants were higher than those in healthy plants. For fungi, the relative abundances of Ascomycota and Fusarium in the rhizosphere of infected A. konjac plants were significantly higher than those of healthy plants, but those of infected A. muelleri plants were lower than those of healthy plants. The relative abundance of beneficial Penicillium fungi was lower in infected A. konjac plants than in healthy plants, and that of infected A. muelleri plants was higher than that of healthy plants. These findings can provide theoretical references for further functional research and utilization of Amorphophallus spp. rhizosphere microbial communities in the future.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.2
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• pp.157-163
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• 2001

Introgression from genetically modified plants (GMPs) may be dependent on the genetic similarity to wild relative plants. In Korea, many wild plant species are botanically related to the cultivated plants that have a potential to be genetically transformed. The controversy for hazards of GMPs is continuing because the studies on gene flow or introgression are little. Based on the systematic criteria, we have surveyed Korean wild plant species that showed the similarity to cultivating crops. The consideration for feasibility of genetic pollution (introgression of transgene) is necessary for the successful accomplishment in the practical use of GMPs. Although the detrimental effects of GMPs on wild relatives have not been clearly verified, Korean wild plant species related to crop plant (potential GMP) have to be investigated with respect to the introgression. Korean flora consists of ca. 5,500 species. Among them, 1,448 species are classified as weed species (966 native, 325 naturalized, and 167 escaped ones), which is vulnerable to GMPs in term of introgression. We suggested the principal Korean wild plants related to major crops that might be affected by GMPs via introgression. The investigated species herein are selected based on the morphological and phenological relationship. It is necessary to verify the genetic relationship between cultivated plants and wild relatives sing more precise molecular techniques, which provide the information of likelihood for the introgression of transgene.

• Korean Journal of Environment and Ecology
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• v.26 no.6
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• pp.839-867
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• 2012

Vascular plant surveys in Iss. Youngjongdo and Yongyudo, Incheon were conducted 6 times from May to October 2009 and July 2011. The vascular plants inventoried during the surveys were 644 taxa in total; 11 formas, 73 varieties, 4 subspecies, 556 species, 352 genera and 98 families. Among them, 250 taxa were newly found from the survey areas, including Cnidium japonicum Miq., Argusia sibirica (L.) Dandy, Breea setosa (Willd.) Kitam., Dryopteris erythrosora (D. C. Eaton) Kuntze and Dryopteris nipponensis Koidzumi. In the case of dormancy form, hemicryptophytes were most abundant by 37.1%. Six taxa of Korean endemic plants and nine Korean rare plants were also newly recognized along with 45 taxa of notable plants of regional flora announced by the Ministry of Environment. The naturalized plants were 95 taxa, and the Urbanization Index of the survey areas was 29.6%.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.1
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• pp.25-35
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• 2015

Pennisetum aloperculoides is a key revegetation species mixed with other plants species and used for revegetating cut-slopes. The purpose of this research is to identify the effects of mixed seeding of revegetation plants on cut-slope revegetation with respect to the quantity of Pennisetum aloperculoides seeds. The coverage ratio and appearance frequency of Pennisetum aloperculoides, and other revegetation species were measured to assess the cut-slope revegetation. We divided Pennisetum treatments into four groups with different Pennisetum seed quantities of $0g/m^2$, $5g/m^2$, $10g/m^2$, and $15g/m^2$. For each treatment group, we mixed identical quantities of seeds from herbaceous flowers (bird's-boot trefoil, aster, chrysanthemums, golden coreopsis and china pink), cool-season turfgrasses, and woody plants (korean lespedeza, indigo and silk tree). The increase in the quantity of the Pennisetum seeds resulted in the higher coverage ratio for Pennisetum, but in the lower coverage ratio for herbaceous flowers, cool-season turfgrasses, and woody plants. We observed a short-term succession process in which the dominant species shifted in the following order: the initial species Pennisetum, herbaceous flowers, and then lastly woody plants. In case of the appearance frequency, we also observed the higher appearance frequency for Pennisetum and the lower appearance frequency for the other plants due to the increase in the quantity of Pennisetum seeds. Pennisetum, bird's-foot trefoil and china pink showed the tendency to decrease the appearance frequency from one month after seeding while cool-season turfgrasses became extinct due to summer drought. In the woody plants, the appearance frequencies of korean lespedeza and indigo were high due to the decrease in quantity of Pennisetum seeds. The silk trees were damaged from winter frost and none emerged at all in 2013 (the following year after the seeding). Korean lespedeza and indigo appeared to have the short-term rapid dominance over other treated revegetation plants.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.597-612
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• 2013

The flora of Is. Gageodo (N $34^{\circ}02^{\prime}{\sim}34^{\circ}05^{\prime}$, E $125^{\circ}05^{\prime}{\sim}125^{\circ}08^{\prime}$) in Sinangun (Province Jeollanamdo) was surveyed from April 2010 to October 2011. The vascular plants from the results of 23 field surveys were revealed as 385 taxa; in total 98 families, 258 genera, 348 species, three subspecies, 31 varieties and four forms. Fifty taxa were the first records in this region. The plants in various categories were discovered in this study. The number of Korean endemic plants were 7 taxa, and 8 taxa designated by Korean Forest Service as rare plants were investigated in this region. The plants above the third degree among the floristic regional indicator plants designated by Korean Ministry of Environment were 30 taxa. In addition, 28 taxa of the naturalized plants were recorded.

• Korean Journal of Environment and Ecology
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• v.29 no.2
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• pp.105-131
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• 2015

This study was carried out to investigate the flora of Gyodongdo island (Ganghwa-gun). The vascular plants from 11 field surveys were revealed to belong to a total of 629 taxa; 118 families, 364 genera, 561 species, 5 subspecies, 53 varieties, 7 forms and 3 hybrids. 184 taxa were the first records from this region. The plants in Gyodongdo island are composed of the deciduous broad-leaved and conifer-mixed forests which are the common ones in the middle part of the Korean Peninsula. Five taxa of Korean endemic plants such as Clematis brachyura Maxim., Viola seoulensis Nakai, Populus ${\times}$ tomentiglandulosa T. B. Lee, Forsythia koreana (Rehder) Nakai and Hemerocallis hakuunensis Nakai were collected. Endangered wild plants designated by the law called 'Protection Law for Endangered wild fauna and flora' were one taxon. The red list plants according to IUCN valuation basis were examined for 13 taxa; endangered (EN) species of Prunus yedoensis Matsum., Vulnerable (VU) species of both Utricularia pilosa (Makino) Makino and Iris ruthenica var. nana Maxim., Near Threatened (NT) species of Senecio argunensis Turcz., Least Concern (LC) species of Platycladus orientalis (L.) Franco, Potentilla discolor Bunge, Limnophila sessiliflora (Vahl) Blume, Acorus calamus L., Phacelurus latifolius (Steud.) Ohwi, Pseudoraphis ukishiba Ohwi, Belamcanda chinensis (L.) DC., and Not Evaluate (NE) species of both Astragalus sikokianus Nakai and Potamogeton oxyphyllus Miq. The floristic regional indicator plants found in this area were a total of 47 taxa comprising three taxa of grade V, four taxa of grade IV, nine taxa of grade III, 10 taxa of grade II, and 21 taxa of grade I. The naturalized plants were identified as 62 taxa and the percentage of naturalized index (NI) was 9.9 % and the percentage of urbanization index (UI) was 19.3 %, respectively. Furthermore, hemicryptophytes (28 %), therophytes (26 %), hydrophytes (13 %) and geophyte (12 %) showed high proportional ratio in life form spectrum.