• Mycobiology
• /
• v.43 no.1
• /
• pp.14-23
• /
• 2015

The influences of temperature and nutritional conditions on the mycelium growth of oyster mushroom Pleurotus ostreatus (PO) and Pleurotus cystidiosus (PC) were investigated in laboratory experiment during the summer season of 2014. The results of the experiment indicated that potato dextrose agar (PDA) and yam dextrose agar (YDA) were the most suitable media for the mycelium growth of oyster mushroom PO while four media (PDA, YDA, sweet potato dextrose agar, and malt extract agar medium) were not significantly different in supporting mycelium growth of oyster mushroom PC. The optimal temperature for mycelium growth of both oyster mushroom species was obtained at $28^{\circ}C$. Mycelium growth of oyster mushroom PO was improved by carbon sources such as glucose, molasses, and at 1~5% sucrose concentration, mycelium colony diameter of mushroom PO was achieved the highest value. Whereas glucose, dextrose, and sucrose as carbon sources gave the good mycelium growth of oyster mushroom PC, and at 1~3% sucrose concentration, mycelium colony diameter of PC was achieved the maximum value. Ammonium chloride concentrations at 0.03~0.09% and 0.03~0.05% also gave the greatest values in mycelium colony diameter of mushroom PO and PC. Brown rice was found to be the most favourable for mycelium growth of two oyster mushroom species. In addition, sugarcane residue, acasia sawdust and corn cob were selected as favourable lignocellulosic substrate sources for mycelium growth of both oyster mushrooms.

• The Plant Pathology Journal
• /
• v.21 no.3
• /
• pp.221-228
• /
• 2005

A total of 179 isolates of Trichoderma spp. were collected from oyster mushroom substrates in Korea. On the basis of morphological and cultural characteristics, Trichoderma isolates were divided into seven groups, namely T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, referred to as Trichoderma sp. 1 and 2. The predominant species was Trichoderma sp. 2 (n=86) followed by Trichoderma sp. 1 (n=52). Trichoderma sp. 1 and 2 were morphologically distinct not only from the other species of Trichoderma reported but also from each other in the characteristics such as mycelial growth rate, colony appearance, shape of conidia and conidiophores and branching pattern of phialides, although branching pattern of phialides of Trichoderma sp. 1 was similar to that of T. harzianum. In virulence test, the degree for compost colonization of Trichoderma sp. 2 was significantly greater than that of the other Trichoderma species. Trichoderma sp. 2 was found to be the main cause of green mold disease in oyster mushroom production. More work including molecular characterization is needed to confirm the species of Trichoderma sp. 1 and 2.

• Fisheries and Aquatic Sciences
• /
• v.11 no.1
• /
• pp.32-35
• /
• 2008

The Suminoe oyster, Crassostrea ariakensis, occurs in estuaries where rivers meet seawater. In Korea, it is one of the most popular fisheries resources in the Nam River and Sumjin River. However, the genetic identification of this species has been questioned, because specimens are often mis-identified as other species. To identify the species, we conducted polymerase chain reaction (PCR) amplification of the internal transcribed spacer-1 (ITS-1) region, followed by digestion with the restriction enzyme HaeIII. Restriction profiles for oysters collected from Korea, Japan, and China (north and south) were determined by comparing the PCR-restriction fragment length polymorphism (RFLP) patterns of the ITS-1 regions. Our study verified that the oysters collected from Korea were C. ariakensis based on the PCR-RFLP patterns. These results emphasize the value of molecular markers for identifying morphologically uncertain species.

• Preventive Nutrition and Food Science
• /
• v.18 no.1
• /
• pp.23-29
• /
• 2013

This study was designed to investigate the anti-inflammatory effect of oyster shell extract on the production of pro-inflammatory factors [NO, reactive oxygen species (ROS), nuclear factor-kappa B (NF-${\kappa}B$), inducible nitric oxide synthase (iNOS) and cycloxygenase-2 (COX-2)] and pro-inflammatory cytokines [Interleukin-$1{\beta}$ (IL-$1{\beta}$), Interleukin-6 (IL-6) and TNF-${\alpha}$] in the lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. Cell viability, as measured by the MTT assay, showed that oyster shell extract had no significant cytotoxicity in Raw 264.7 cells. The treatment with oyster shell extract decreased the generation of intracellular reactive oxygen species dose dependently and increased antioxidant enzyme activities, such as SOD, catalase, GSH-px in LPS-stimulated macrophage cells. Oyster shell extract significantly suppressed the production of NO and also decreased the expressions of iNOS, COX-2 and NF-${\kappa}B$. Additionally, oyster shell extract significantly inhibited the production of IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ in LPS-stimulated Raw 264.7 cells. Thus, these results showed that the oyster shell extract had an anti-inflammatory effect on LPS-stimulated Raw 264.7 cells.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.127.1-127
• /
• 2003

We describe two new Trichoderma species associated with oyster mushroom in Korea. Trichoderma green mould has been one of the most serious diseases of oyster mushroom in Korea. Of these the predominant species are two unrecorded species. We designed as Trichoderma sp. Korean type 1 (Th K1) and Trichoderma sp. Korean type 2 (Th K2), respectively. Th K1 and Th K2 can be distinguished from previously reported Trichoderma species as well as each other in morphological characteristics including growth rate at 35$^{\circ}C$, colony morphology, conidia shape and branch pattern of phialides. Sequence of the ITS region of rDNA, the protein coding translation elongation factor gene(EF-1${\alpha}$), and RNA polymeraseII (RPB2) not only clearly separated Trichoderma sp. Korean types from their closely related T. harzianum biotype but also distinguished them from each other. Analyses of the EF-1${\alpha}$ and RPB2 sequences were found to be more useful for establishing systematic relationships among Trichoderma isolates than those of the ITS sequence. Based on the results of morphological and molecular characteristics. We propose the two Trichoderma sp. Korean types as the new species

• The Korean Journal of Malacology
• /
• v.30 no.3
• /
• pp.249-258
• /
• 2014

Phytoplankton species composition and ecological index (diversity, evenness, richness and dominance) were analysed from April 2013 to March 2014 at 10 stations of cupped oyster, Crassostrea gigas culture area in Iwon coast, Korea. Seasonal and positional variation of phytoplankton standing crops, biomass, dominant species and water quality were distinctively different according to occasionally inflow of Iwon dam reservoired water. The composition of phytoplankton species were Bacillariophyceae 98, Dinophycease 22, Chlorophycease 13, Cyanophyceae 8, Silicofalgellate 4, Euglenophyceae 2, Cryptophyceae 1 species. The most dominant species was Bacillariophyceae as 64.0%. The highest biomass of phytoplankton recorded in September as $40,910{\times}10^3$ cell/L at the station 1, near from inland water inflow area. Ecological indices (diversity, richness, evenness, and dominance index), used for structural change of phytoplankton community and water quality (temperature, dissolved oxygen, salinity) showed difference of spatiotemporal property also.

• The Plant Pathology Journal
• /
• v.21 no.3
• /
• pp.229-236
• /
• 2005

Molecular profIles of PCR-RFLP and sequence analysis of internal transcribed spacer (ITS) regions of rDNA were compared between morphologically distinguishable species of Trichoderma isolated from substrates of oyster mushroom in Korea, T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, Trichoderma sp. 1 and 2. PCR­RFLP analysis divided the Trichoderma spp. into six RFLP groups, A, B, C, D, E, and F. The RFLP groups were generally agreed with described morphological species, except that the RFLP group A containing the two unidentified species. A neighbor-joining tree based on ITS sequences well supported RFLP groups observed by RFLP analysis of ITS regions of rDNA. Additionally, the two unidentified species, Trichoderma sp. 1 and 2, which could not be distinguished by PCR­RFLP analysis, were separated in sequence analysis of ITS regions of rDNA.

• Journal of Advanced Marine Engineering and Technology
• /
• v.32 no.3
• /
• pp.387-393
• /
• 2008

This paper has dealt with the probability of oyster shell desiccant cooling system driven by renewable energy of photo-volatic effect with fundamental experiment. The test materials for desiccant are activated charcoal, silica-gel, hi-dry, and oyster shell. The experiments were mainly performed with focusing on the observation of surface features, adsorption amounts of the adsorbent species, and the effect of temperature. Oyster shell has sufficient probability for using as desiccant in a air-conditioning system. Moreover, the heat releasing device would be attached in the system, the system based with oyster shell can be operated with high efficiency.

• Mycobiology
• /
• v.34 no.3
• /
• pp.111-113
• /
• 2006

This paper describes and illustrates two new species, Trichoderma pleurotum and T. pleuroticola, associated with green mold disease of oyster mushroom in Korea.

• Journal of Species Research
• /
• v.3 no.2
• /
• pp.123-126
• /
• 2014

We present a list of the beetle species associated with the oyster mushroom, Pleurotus ostreatus Fr. in the birch forest of Onon-Balj National Park in Mongolia. A total of 21 species in seven families were found in this fungus, including fungivorous species: Litargus japonicus Reitter and Mycetophagus antennatus (Reitter) (Mycetophagidae), Pseudamblyopus similis (Lewis) (Erotylidae), and Lordithon sp. (Staphylinidae) etc. Both adult and larval forms are presented in the checklist along with the type of fungal association, of which approximately 52 percent are obligate mycetobionts. The main trends of the ecological and trophic specialization of the mycetophilic beetles are discussed.