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Growth Inhibition against Contaminants in Aseptic Chocolate Milk Using Physicochemical Methods (물리.화학적 처리에 의한 멸균 초콜릿 우유 오염균의 생육억제 효과)

  • Choi, Moon-Kyoung;Yoon, So-Young;Lee, So-Young;Kim, Koth-Bong-Woo-Ri;Lee, Chung-Jo;Jung, Ji-Yeon;Kwak, Ji-Hee;Kim, Min-Ji;Kim, Dong-Hyun;SunWoo, Chan;Lee, Ju-Woon;Byun, Myung-Woo;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.8
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    • pp.1157-1163
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    • 2011
  • This study was conducted to investigate the cause of microbiological contaminants in aseptic chocolate milk and evaluate the effect of a physicochemical treatment on the growth inhibition of isolated bacterial strains. The bacterium isolated from aseptic chocolate milk was identified as Bacillus lentus and was named B. lentus M1. In the heat and pH treatment, the growth of B. lentus was inhibited at 110$^{\circ}C$ for >15 min and at pH's <5 and >10. An electrolyzed water treatment against B. lentus M1, revealed 5 mm growth past the inhibition zone. The effect of ozone gas on B. lentus M1 growth was evaluated using viable cell counts. When the initial number of B. lentus M1 was $10^2$ and $10^3$ CFU, the bacteria were completely suppressed by ozone gas treatment for 10 and 30 min, respectively. In a microwave treatment, B. lentus M1 was sterilized following microwave treatment for 1 min. As the result of ${\gamma}$-irradiation against B. lentus M1, numbers decreased as the ${\gamma}$-irradiation dosage increased. These results show the growth inhibition effects against contaminants in aseptic chocolate milk using physicochemical treatments.

A Comparative Study on the Perception of A Beautiful Landscape According to the Differences of Living Environment (성장환경의 차이에 따른 아름다운 경관 인지의 비교연구 -도시와 농촌 국민학생을 대상으로-)

  • 성현찬;임승빈
    • Journal of the Korean Institute of Landscape Architecture
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    • v.20 no.3
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    • pp.64-78
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    • 1992
  • In this study, elementary school students of both urban and rural areas as its subjects were asked to draw 'A beautiful landscape' by employing the perspective representation technique, i. e., the Perception Map, and to write down the elements comprising 'A beautiful landscape' in the questionnaire sheets. By doing so, an attempt was made 1) to analyze whether there are differences in perceiving 'A beautiful landscape' according to the differences of the environment in which they were brought up ; and, if there are differences. 2) to identify them ; and based on that , 3) to present basic data for evaluation on landscape, on its preference analysis and for Park Planning. The summary of this study is as follows ; 1) The main elements, elementary school students think, comprising 'A beautiful landscape' are 25 ones such as Sky(7), Sea(2), Water(2), Topography(5), Plants(5), Animals(3), School(1), Rural village(1). The natural elements showing a difference are ; Water fall in urban areas and School landscape in rural areas ; the artificial elements are ; City groups(Structures, Facilities, Necessities, Transportation means and Space) in urban areas and School groups in rural areas. Especially, in case of rural area children, they regard 'Trees' as an essential element to be 'A beautiful landscape' comparing to those in urban areas. 2) According to the analysis result on the correlation between the elements comprising a beautiful rural landscape and a beautiful ruban landscape, the correlation between boys and girls is high, showing the same trend with any difference. In comparison of urban areas with rural areas, there is no difference between natural elements, but in artificial elements(7 groups without family) the correlation is quite low, showing that all comprising elements are not the same between rural schools and cities, between schools within the same areas, and between schools of different areas. 3) In identifying the names of elements comprising 'A beautiful landscape', Back-Du Mountain and Sorak Mountain are shown the highest frequency in the category of mountains. In the names of trees and flowers, the elementary school children are thought to consider the kinds of trees and flowers they can see always at hand, i. e., those in their school ground where they spend most of their day time. 4) In the analysis of the numbers of comprising elements according to the responses in the questionnaire sheets and in the Perception Map, 'less than 10' is the most frequently counted number of comprising elements by individual students regardless of rural and urban differences. When the total frequency is divided by the number of students, the mean score is 6-7 without any differences between rural and urban areas, implying that there are no differences in the expression ability between urban and rural schools. 5) According to the result of classyfying and analysizing the landscape appeared on the Perception Map by similar elements and by similar scenes, 'A beautiful landscape' thought by elementary school children is defined not as a standardized form but as 11 types such as the landscape of fields, the landscape of a sea, the landscape of a rural village, a type where elements are assembled, the landscape of cities, the landscape of a school, the landscape coming out of a imagination, and other landscape. Both rural and urban children all consider the landscape of mountains and field and the landscape where several elements are assembled as a commonly beautiful one. Among the landscapes showing rural and urban differences, it can be analyzed that urban children regard the landscapes of cities, imagination, and waterfalls as something characteristic, while rural children regard the landscape of schools and rural villages as something characteristic.

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Two Crystal Structures of the Vacuum-Dehydrated Fully $Ag^+$-Exchanged Zeolite X ($Ag^+$ 이온으로 완전히 치환되고 탈수된 두개의 제올라이트 X의 결정구조)

  • Jang, Se Bok;Park, Sang Yun;Song, Seong Hwan;Jeong, Mi Suk;Kim, Yang
    • Journal of the Korean Chemical Society
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    • v.40 no.7
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    • pp.474-482
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    • 1996
  • Two crystal structures of the vacuum dehydrated $Ag^+$-exchanged zeolite X have been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd3 at 21(1)$^{\circ}C$ (a=24.922(1)${\AA}$ and a=24.901(1)${\AA}$, respectively). Each crystal was ion exchanged in flowing streams of aqueous $AgNO_3$ for three days. The first crystal was dehydrated at 300$^{\circ}C$ and $2{\times}10^{-6$torr for two days. The second crystal was similarly dehydrated at 350$^{\circ}C$. Their structures were refined to the final error indices, $R_1=0.095\;and\;R_2=0.092$ with 227 reflections, and $R_1=0.096\;and\;R_2=0.087$ with 334 reflections, respectively, for which I > 3${\sigma}$(I). In the first crystal, Ag species are found at five different crystallographic sites: sixteen $Ag^+$ ions fill the site I, the center of the double 6-ring, thirty-two Ag0 atoms fill the I' site in the sodalite cavities opposite double six-rings, seventeen $Ag^+$ ions lie at the 32-fold site II' inside the sodalite cavity at the single six-oxygen ring in the supercage, fifteen Ag+ ions lie at the 32-fold site II, in the supercage, and the remaining twelve $Ag^+$ ions lie at site III' in the supercage at a little off two-fold axes. In the second crystal, all Ag species are located similarly as crystal 1; 16 at site I, 28 at site I', 16 at site II, 16 at site II', 6 at site III and 6 at site III'. Total 88 silver species were found per unit cell. The remaining four Ag atoms were migrated out of the zeolite framework to form small silver crystallites on the surface of the zeolite single crystal. In the first structure, the numbers of Ag atoms per unit cell are approximately 32.0 and these may form tetrahedral $Ag_4$ clusters at the centers of the sodalite cavities. The probable four-atom cluster is stabilized by coordination to two $Ag^+$ ions. The Ag-Ag distance in the cluster, ca. 3.05 ${\AA}$, is a little longer than 2.89 ${\AA}$, Ag-Ag distance in silver metal. At least two six-ring $Ag^+$ ions on sodalite cavity (site II') must necessarily approach this cluster and this cluster may be viewed as a distorted octahedral silver cluster, (Ag6)2+.

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Exploring on the Defense Strategies against Hervivory of Broad-leaved Tree Species Growing in Taean-gun, Chuncheongnam-do (충남 태안군에서 생육중인 활엽수종의 초식에 대한 방어전략 탐색)

  • Kim, Gab-Tae;Choo, Gab-Cheul;Lyu, Dong-Pyo;Um, Tae-Won
    • Korean Journal of Environment and Ecology
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    • v.26 no.1
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    • pp.39-45
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    • 2012
  • To explore on the defense strategies against hervivory of 67 broadleaved tree species, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves, collected from the trees growing in Taean-gun, Chuncheongnam-do were investigated. 46 broadleaved tree species(68.7%) had the domatia structures, and 21 species including Quercus salicina and Magnolia grandiflora did not. 31 species including Juglans mandshurica and Carpinus laxiflora reveals tuft type, 12 species including Quercus dentata and Corylus heterophylla reveals pocket+tuft type, and 2 species, Sorbus alnifolia and Prunus yedoensis does pocket type, and Viburnum odoratissimum var. awabuki does pouch type. Domatia number per leaf proves the highest figures, 23.4/leaf for Quercus dentata. Plant defense strategies using leaf lower-surface trichomes of Magnolia grandiflora reveals dense villous, those of Populus alba and Vitis vinifera reveals dense pilose, that of Elaeagnus umbellata does dense scaly hairs, that of Pueraria lobata does dense strigose. Plant defense strategies using extrafloral nectaries were adapted 23 tree species(34.3%). Observed examples are Prunus tomentosa, Ficus carica, Viburnum dilatatum and Carpinus laxiflora. Predatory mites were observed on the leaves of 40 tree species(59.7%), and mean values of predatory mites was highest values 23.4/leaf in Quercus dentata. Minute arthropods destroying the leaf of broadleaved trees. are such as Periphyllus californiensis, P. viridis, Diaspididae sp., gall mites, thrips, and total numbers observed were odered gall mites, Diaspididae sp., aphids and thrips. Natural enemies of these hervivores arthropods are such as predatory mites, Chilocorus rubidus, Coccinella septempunctata and the nymph, Aphidius ervi. These results indicate that defense strategies including protective mutualisms may be frequent in the temperate broadleave trees.

The Analysis of Visiting Patterns for the Top of Seoseokdae in Mudeungsan National Park (무등산국립공원 서석대 정상부의 탐방패턴 분석)

  • Shim, Seok-Yeong;Park, Seok-Gon
    • Korean Journal of Environment and Ecology
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    • v.31 no.2
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    • pp.266-274
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    • 2017
  • The purpose of this study was to analyze the number of visitors to the top of Seoseokdae in Mudeungsan National Park, in which visitors are concentrated, and their visiting patterns, thereby suggesting measures to manage the visitors. The number of annual visitors and the numbers of regional and seasonal visitors to Mudeungsan National Park, which affect the concentration of visits to Seoseokdae were analyzed using the data produced by an automatic digitizing device. A field study was conducted to examine the number of seasonal and periodic visitors to Seoseokdae and their visiting patterns. In 2015, the number of visitors was 2,563,651 and 83.9% of the visitors visited via the Jeungsimsa and Wonhyosa area that is near Gwangju City. This area is close to the Seoseokdae area and it is easy to hike between the areas. Therefore, there was an influx of most visitors to Seoseokdae into the Jeungsimsa and Wonhyosa area. In terms of seasonal visitors, the largest number of visitors came in the fall, followed by the summer, spring, and winter in order. However, the seasonal differences were not notable. There was no statistically significant correlation between the number of visitors and meteorological factors. This result may have been because Gwangju citizens frequently visit Mudeungsan regardless of period and weather. Visitors can get to Seoseokdae via the trails into Wonhyosa and Jangbuljae. A slightly larger number of visitors used the trail into Jangbuljae in the fall and winter, whereas a larger number of visitors used the trail into Wonhyosa in the summer. In general, there is a large influx of visitors into Jangbuljae, a strategic visiting point. However, a slightly larger number of visitors may have chosen the trail into Wonhyosa in the summer because they could hike under the shade of trees. In the summer, visitors stayed in Seoseokdae for a short time with a low level of crowdedness. On the other hand, in the fall and winter, visitors stayed in the area longer because they had lunch and rested. During the time, the number of momentary maximum visitors peaked, causing extreme crowdedness. Therefore, some visitors showed the visiting pattern of entering the grassland outside the designate zone. Because this behavior can damage the grassland on the top of Seoseokdae, which can lead to soil erosion, intensive visitor management may be necessary.

A Study on the Population Fluctuation of Wintering Waterbirds on Wonju-Stream by the Temperature (원주천 월동 수조류의 기온에 따른 군집 변동에 관한 연구)

  • Park, Yung-Wook;Lee, Hwang-Goo;Choi, Jun-Kil
    • Korean Journal of Environment and Ecology
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    • v.31 no.2
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    • pp.135-151
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    • 2017
  • Waterbirds were surveyed once a week from Oct. 2015 to Apr. 2016 to study the conditions of temperature of staying waterbirds on Wonju-Stream. The population staying on Wonju-Stream was in inverse proportion to the temperature change; birds increased as the temperature went down, and showed the highest peak counts on the 16th(27-Jan) survey, which was the lowest in temperature. decreasing as the temperature rose. The total population of Wonju River water birds increased with increasing freezing rate in proportion to the freezing rate of nearby reservoirs and tributaries. To clarify the relation of the numbers of the birds to temperature fluctuation, the correlation of the first period of wintering (1st~16th) and that of the later period(17th~27th) as well as the whole period was surveyed, based on the 16th which was the lowest in temperature. While most wintering waterbirds showed a negative correlation to the temperature, Egretta garzetta, Phalacrocorax carbo, and Ardea cinerea were positive in temperature relativity. Anas poecilorhyncha was in the highest relation to temperature fluctuation showing (7D/H3A(r=-0.960, P<0.000000001)) at 1-16 correlation(Cor.), with the whole wintering waterbird species showing the maximum correlation at 4D/LA in the every correlation(1~27Cor.(r=-0.942, P<0.01), 1~16Cor.(r=-0.947, P<0.01), 16~27Cor.(r=-0.958, P<0.01)). Each waterbird showed the correlation to the temperature variation(AT, HT, LT) and th duration(1D~7D) depending on the species differently. Most species demonstrated a bigger correlation to the lowest temperature rather than to the highest temperature. During the first half period of the wintering with the temperature falling it showed a high correlation to the temperature at the duration of 4D~7D, While it was for D1~D3 during the next half period.

Seasonal Occurrences of the Apple Leaf Miner, Phyllonorycter ringoniella (Matsumura) and Its Parasites and Damaging Leaf Position (사과굴나방 및 그기생충류(寄生蟲類)의 발생소장(發生消長)과 가해엽위(加害葉位))

  • Lee, Soon-Won;Kim, In-Soo;Kim, Jeong-Hwan;Lee, Moon-Hong;Hyun, Jai-Sun
    • Korean journal of applied entomology
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    • v.24 no.3 s.64
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    • pp.151-156
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    • 1985
  • Apple leaf miner(ALM), Phyllonorycter ringoniella(Matsumura), occurs $4{\sim}5$ generations a year in Suweon; adult emergence peaks being in mid April, early June, early July, mid August, and mid September with the highest one in the 4th generation. Numbers of days required to complete the development(egg to adult emergence) were different with oviposited dates; 43days for late May, $32{\sim}37$ days for June to July, and 39days for early August. ALM larvae oviposited after late August did not emerge and went into diapause. Sites of the infested leaf on the shoot seemed to be somewhat different with the ALM generations; the preferred leaf sites being the 1st-6th leaf for the 1st, the 4th-9th for the 2nd, the 4th-18th for the 3rd or 4th, and the terminal leaves of the first growth shoot or the leaves of the secondary shoot for the 5th generation. Parasites of three families emerged from the mines of ALM in Suweon. The encyrtid (Holcothorax testaceipes Ratzelburg) occurs 4 generations and the eulophids 5 generations a year. A small number of the braconid(Apanteles sp.) occcrred only in September.

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Static and Dynamic Analysis for Railway Tunnel according to Filling Materials for overbroken tunnel bottom (철도터널 하부 여굴처리 방법에 대한 정적 및 동적 안정성 검토)

  • Seo, Jae-Won;Cho, Kook-Hwan
    • Journal of the Korean Society for Railway
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    • v.20 no.5
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    • pp.668-682
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    • 2017
  • Alignments of railways recently constructed in Korea have been straightened due to the advent of high-speed rail, which means increasing the numbers of tunnels and bridges. Overbreak during tunnel construction may be unavoidable, and is very influential on overall stability. Over-excavation in tunneling is also one of the most important factors in construction costs. Overbreak problems around crown areas have decreased with improvements of excavation methods, but overbreak problems around bottom areas have not decreased because those areas are not very influential on tunnel stability compared with crown areas. The filling costs of 10 cm thickness of overbreak at the bottom of a tunnel are covered under construction costs by Korea Railway Authority regulations, but filling costs for more than the covered thickness are considered losses of construction cost. The filling material for overbreak bottoms of tunnels should be concrete, but concrete and mixed granular materials with fractured rock are also used for some sites. Tunnels in which granular materials with fractured rock are used may have a discontinuous section under the concrete slab track. The discontinuous section influences the propagation of waves generated from train operation. When the bottom of a tunnel is filled with only concrete material, the bottom of the tunnel can be considered as a continuous section, in which the waves generated from a train may propagate without reflection waves. However, a discontinuous section filled with mixed granular materials may reflect waves, which can cause resonance of vibration. The filled materials and vibration propagation characteristics are studied in this research. Tunnel bottom filling materials that have ratios of granular material to concrete of 5.0 %, 11.5 %, and 18.0 % are investigated. Samples were made and tested to determine their material properties. Static numerical analyses were performed using the FEM program under train operation load; test results were found to satisfy the stability requirements. However, dynamic analysis results show that some mixed ratios may generate resonance vibration from train operation at certain speeds.

Comparison of physiochemical property, phytochemical contents, and biological activity of soy sauce added with bitter melon powder (여주분말 함유 간장의 이화학적 특성, phytochemical 함량 및 생리활성 비교)

  • Hwang, Chung Eun;Lee, Dong Hee;Joo, Ok Soo;Lee, Hee Yul;Kim, Su Cheol;Park, Kyung Sook;Um, Bong Sik;Cho, Kye Man
    • Food Science and Preservation
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    • v.24 no.8
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    • pp.1138-1148
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    • 2017
  • In this study, contetnts of phenolic acid and isoflavone, and biological activities of soy sauce were compared the soy sauce added bitter melon powder (BMPs). After the fermentation, pHs were decreased from 5.83 (0% BMP), 5.47 (5% BMP), and 5.32 (10% BMP) to 5.28, 5.36, and 5.16 at 90 days, whereas the acidities of soy sauce were increased from 0.06%, 0.07%, and 0.09% to 0.30%, 0.28%, and 0.36% at 90 days, respectively. In addition, the salinities of soy sauce were decreased, while viable cell numbers including Bacillus and yeast were increased. The contents of total phenolic, isoflavone-aglycone, and phenolic acid and antioxidant and ${\alpha}$-glucosidase inhibition activities were significantly increased for 90 days, while the isoflavone-glycoside contents were decreased. In Particular, soy sauce with 10% BMP at 90 days showed the highest contents of glutamic acid (GA, 9,876.09 mg/100 mL) and ${\gamma}$-aminobutyric acid (GABA, 325.02 mg/100 mL) contents than among other samples. Additionally, the radical scavenging activities (DPPH, ABTS, ${\cdot}OH$, and FRAP) and ${\alpha}$-glucosidase inhibition activities of soy sauce with 10% BMP at 90 days were shown to be high 96.07%, 97.27%, 59.47%, 1.98%, and 79.96%, respectively.

Influence of the Food Intake Ability and the Number of Remaining Teeth on Oral Health Related Quality of Life in Some Elderly People (일부 노인의 저작강도별 음식섭취능력과 잔존치아수가 구강건강관련 삶의 질에 미치는 영향)

  • Kim, Da-Hye;Hwang, Soo-Jeong
    • Journal of dental hygiene science
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    • v.16 no.1
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    • pp.53-61
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    • 2016
  • Eating supports to gain energy and nutrition and improves quality of life. Ageing affects the food intake ability due to loss of natural teeth and the progressive muscle degeneration. Our objective was to investigate how the food intake ability (FIA) and the remaining teeth could influence on oral health related quality of life in the elderly. This study was performed with 503 elderly subjects living in Daejeon, Korea. The questionnaire with the FIA with 30 Korean food and Oral Health Impact Profile 14 (OHIP-14) and oral examination were surveyed. The five groups according to cluster analysis of FIA had the different numbers of remaining teeth and functional posterior teeth with opposing teeth or prosthesis significantly: group 1, $21.78{\pm}8.27$ and $2.80{\pm}2.63$; group 2, $16.75{\pm}7.87$ and $2.16{\pm}2.44$; group 3, $14.68{\pm}9.77$ and $1.73{\pm}2.30$; group 4, $9.93{\pm}8.13$ and $0.78{\pm}1.68$; group 5, $10.18{\pm}8.37$ and $0.51{\pm}1.22$. The more foods the subjects could masticate, the better oral health related quality of life they had. The medium FIA, soft FIA and the number of remaining teeth could explain 46% of OHIP-14, but hard FIA could not in the multiple regression model. We suggested to develop the oral health program for the elderly to be able to eat the food with medium physical property at least be helpful to improve oral health related quality of life.