• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.180-181
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• 2001

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.2
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• pp.131-141
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• 2005

Molecular-based genetic diversity for a set of 141 accessions of Korean barley cultivars and 24 accessions of foreign exotic cultivars were analyzed using random amplified polymorphic DNAs (RAPDs). Different level of genetic variability was observed with 30 random decamer primers in the Korean barley varieties and breeding lines which were preliminarily classified by morphological (hulled & hulless barley) and end-use (malting barley) and/or by the released periods. A total of 74 RAPD bands were scored, and the number of bands per primer varied from 1 to 7 with an average of 2.74. The hulled barley pool had one more marker genotype per primer than the hulless barley pool. The polymorphic information content (PIC) values based on the band pattern frequencies among genotypes varied depending on genetic pools where mean PICs of hulled, hulless and malting barleys were 0.62, 0.57, and 0.43, respectively. Certain genomic loci amplified by opR04, opF01, opB05, and opC13 were highly polymorphic with PIC>0.8. Patterns and temporal trends of genetic diversity assessed over the period from 1970s to 1990s had a tendency to increase, and in particular, this upward slant was quite clear and significant for the hulless barley pool. In the cluster analysis using genetic similarity matrix calculated from RAPD profiles, two major groups and several small subgroups were classified. Major grouping of materials was not affected by the presence of the husk but by their genetic background and the spike-row type. The validity of information on the genetic diversity and relationships between genotypes will have been reviewed to predict their yield potential.

• Journal of Life Science
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• v.13 no.1
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• pp.29-33
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• 2003

The study was conducted to get genetic information on quantitative characters of $F_1$ in barley through diallel crosses using seven malting barley. The results obtained were summarized as follows. Additive, dominant, maternal, and reciprocal effort were observed in culm length, spike length, awn length, culm diameter, grain length, grain width, ]lumber of grains per spike, and 1000 grain weight. Based on the Vr-Wr graphical analysis, culm length, spike length, awn length, grain length, and number of grains per spike were found to be inherited over dominance. Dominant effects were higher than additive effects in culm length, spike length, awn length, culm diameter, grain length, grain width, number of grains per spike, and 1000 grain weight. The narrow- sense heritability showed high value as 40.06% for spike length.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.326-338
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• 2021

Ultra-performance liquid chromatography (UPLC) was used to assess the hordein protein fraction of malting barley. C-hordeins (barley prolamins) were extracted with 70% ethanol (EtOH) and 55% isopropyl alcohol (IPA, 2-propanol), and B-hordeins were extracted with the same alcohols in 1.0% dithiothreitol (DTT). High molecular weight (HMW) prolamins (D-hordeins) were extracted with 50% IPA with 1M Tris-HCl (pH 8.0). The same protein patterns were observed in both the experimental extraction solutions (EtOH and IPA). However, the patterns of hordein, extracted with EtOH and IPA containing 1.0% DTT, differed slightly. C- and B-hordeins extracted from those solutions were analyzed. Twenty-six malting barley varieties developed in Korea were analyzed using UPLC. The varieties were divided into seven groups according to hordein patterns of retention time 16 min to 18 min, and 20 varieties showed unique patterns.

• Proceedings of the Korean Society of Crop Science Conference
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• 1993.10a
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• pp.16-17
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• 1993

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.2
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• pp.171-181
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• 2021

The purposes of this study were to determine how changes in temperature affect germination rates and growth of hulled and dehulled barley, malting barley, and naked oat plants, and to measure chlorophyll content, photosynthetic efficiency, and secondary compounds (total phenol, total flavonoid, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity) in plants grown at 13℃ or 25℃). Various types of barley seeds were collected from areas with ideal conditions for barley cultivation, hereinafter referred to as IA, and also from areas where barley cultivation is more difficult due to lower temperatures, hereinafter referred to as LTA. Seeds were tested for seed vigor. While there were significant differences in the electrical conductivity values between seeds collected from certain specific areas, no significant differences were evident between IA and LTA seeds, regardless of the type of barley seed. When plants were grown at 25℃, there were no significant differences in germination rates, plant height, root length and shoot fresh weight between plants originating from IA and LTA. However, there were differences in the measured parameters of some specific seeds. Similarly, under the low temperature condition of 13℃, no differences in the emergence rate, plant height, and shoot fresh weight were evident between plants originating from IA or LTA, regardless of the type of barley. However, there were differences between some specific seeds. One parameter that did vary significantly was the emergence date. Hulled barley and malting barley emerged 5 days after sowing, whereas naked oats emerged 7 days after sowing. There were no differences in the chlorophyll content and photosynthetic efficacy, regardless of the type of barley. There were no significant differences in total phenol, total flavonoid content, and DPPH radical scavenging activity between plants originating from IA and LTA, regardless of the type of barley. However, there were differences between some specific seeds. In particular, for malting barley the total flavonoid content differed in the order of Gangjin > Changwon > Haenam = Jeonju > Naju. The results indicate that crop growth, yield and content of secondary compounds in various types of barley may be affected by climate change.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.705-710
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• 1995

In order to find out the effect of rainfall at harvesting season on germination of malting barley, the seeds sampled from Cheju island and southern part of Korea were examined. The germination rate of seeds from Cheju island where rainfall was frequent at harvesting season, ranged from 46 to 71%, even though disease infection of the seeds was not that serious as would be expected. High sugar content of seed was resulted from the degradation of carbohydrates during the harvest season. From TZ test the rainfall - affected seeds were found to be highly viable but in the state of secondary dormancy. Results of cold germination test showed that the seeds were recovered from the secondary dormancy. Therefore, it was recommended that the seeds affected by the rainfall at harvesting season should be utilized after 12 months when the dormancy period terminated. The germination percent of the seeds was significantly enhanced by prechilling and / or 1 ppm gibberellic acid treatment. Different seed lots showed different rate of germination and the degree of dormancy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.489-494
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• 1994

Near Infrared Reflectance Spectroscopy(NIRS) has been used as a tool for the rapid, accurate, protein assay of malting barley. NIRS used in this study was filter type instruments, Neotec 102. The objective of this study was to obtain the best calibration equation, for the rapid, ease and accurate protein content analysis of malting barley using NIRS system. The optimum wavelength for protein content analysis used NIRS were 2095nm, 2095/1941nm, 2095/1941/2282nm, 2905/1941/2282/2086nm, respectively. Mean protein content with this calibration equation in NIRS analysis was 10.59%, while 10.60% in Micro-Kjeldahl one. The range of protein content in Micro-Kjeldahl was 8.66~12.66% and that in NIRS was 8.80~12.35%. When 18 other varieties produced in 1992 were analysed with 2095nm, 2095/1941nm, 2095/1941/2282nm, 2095/1941/2282/2086nm equation, standard deviation of difference (SDD)and standard error of performence(SEP) and $R^2$ values were 0.47, 0.43, 0.95, respectively. Both the mean protein content by Micro-Kjeldahl and by NIRS was 10.25%. With this equation, analysied 31 varities produced in 1993, SDD and SEP and r values were 0.69, 0.67, 0.91, respectively, and that bias value was 0.65. In this analysis, mean protein content by Micro-Kjeldahl was 10.17% and by NIRS was 10.81%. The range of protein content in Micro-Kjeldahl was 7.58~14.29%, What that in NIRS was 8.63~13.93%. After adjusted bias in the best calibration equation, mean protein content of Micro-Kjeldahl was 10.17% and that of NIRS was 10.09%, without variance of SDD, SEP and r values.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.4
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• pp.218-234
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• 2004

Recent warm winters were evaluated for a possible shifting of the northern limit for winter barley cultivation in Korea. Therefore, safe zones for winter barley cultivation were reclassified based on the average and minimum January air temperature in recent years. The results are as follows: By analysis of mean values of January average air temperatures for 30 years (1971-2000), the northern limits for safe cultivation of hulled, naked, and malting barley were Ganghwa - Icheon - Chungju - Chunyang - Goseong, Cheonan - Geumsan - Mungyeong - Andong - Sokcho, and Gwangju - Jangheung - Sancheong - Pohang - Uljin lines, respectively. Meanwhile, based on the January average air temperature of 14 years (1987-2000) with warmer winters, the safe cultivation zone of winter barley shifted northward of the normal (1971-2000). So, the northern limits for hulled, naked, and malting barley were Pocheon - Chuncheon - Wonju - Yangpyeong - Chunyang, Ganghwa - Icheon - Chungju - Uiseong - Goseong, and Gunsan - Suncheon - Jinju - Miryang - Yeongdeok - Uljin lines, respectively. Winter barley cultivars with the strongest tolerance to low temperature can be grown up to the adjacent areas of Taebaek Mountains (that is, Inje, Hongcheon, Jecheon, and Taebaek areas). Based on January mean air temperatures of 10-year return period for 30 years (1971-2000), the northern limits for hulled and naked barley were Boryeong - Namwon - Geochang - Gumi - Goseong and Seocheon - Jeongeup - Hapcheon - Yeongdeok - Sokcho lines, respectively. It ~ppears that malting barley can be cultivated only at southern coastal areas (that is, Busan, Tongyeong, Yeosu, and Wando areas). On the other hand, based on the weather conditions of 14 years (1987-2000) with warmer winters, the northern limits for hulled, naked, and malting barley were Ganghwa - Icheon - Yeongju - Goseong, Seosan - Namwon - Mungyeong - Andong - Sokcho, and Gwangju - Jangheung - Sacheon - Ulsan - Uljin lines, respectively. The northern limit for winter barley cultivars including Olbori with the strongest tolerance to low temperature was the Ganghwa - Wonju - Chungju - Chunyang - Goseong line.

• Proceedings of the Korean Society of Crop Science Conference
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• 1986.06a
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• pp.52-53
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• 1986