• Journal of the Korean Wood Science and Technology
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• v.47 no.4
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• pp.393-407
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• 2019

In this study, we prepared lignocellulosic artificial soil that contains Bacillus subtilis (peat moss/perlite/ steam-exploded oak wood/microbial culture = 3:1:3:3, w/w/w/w) for use in the restoration of damaged soil areas. The prepared lignocellulosic artificial soil was mixed with soil at ratios of 0%, 25%, 50%, 75%, and 100%. These mixed soils were then applied to fields, and the resultant physicochemical properties and their effects on the plant growth of Lespedeza cyrtobotrya were observed. The mixture of the prepared artificial soils (mixed at ratios of 25%-100%) with soil had a bulk densities of <$0.04g/cm^3$, porosities of >85%, pH values between 4.3 and 4.7, electrical conductivities of <0.5 dS/m, C/N ratios between 15.0 and 26.5, organic matter content between 23.6% and 43.2%, and bacterial densities between $157{\times}10^6$ and $624{\times}10^6CFU/g$. In addition, the prepared artificial soils mixed with soil at ratios of 25%-50% exhibited higher plant growth rates for L. cyrtobotrya compared with the control. Overall, we identified positive correlations between the plant growth of L. cyrtobotrya and soil bulk density, porosity, water-holding capacity, C/N ratio, organic matter, and bacterial densities.

• Journal of Korean Society of Forest Science
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• v.111 no.2
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• pp.234-241
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• 2022

We performed this study to determine the foliar nutrient concentration and the nutrient stocks of restoration areas and adjacent Pinus densiflora S. et. Z. (red pine) stands in opencast kaolinite mines in Sancheong-gun, Gyeongsangnam-do, southern Korea. We chose six sites to determine foliage nutrient concentrations and the nutrient stocks of soils (0-10 cm depth). The dominant vegetation planted in restoration areas comprised Quercus acutissima Carruth., P. koraiensis S. et. Z., Festuca arundinacea Schreb., and Lespedeza cuneata G. Don. Invading vegetation in the restoration areas comprised Alnus incana (L.) Medik., Robinia pseudoacacia L., and Lespedeza spp., among others. The carbon and nitrogen stocks at 10 cm soil depth were significantly higher in the red pine stands than those in the restoration areas, whereas those of phosphorus, potassium, and magnesium were not significantly different between the two areas. However, calcium stocks were significantly higher in the restoration areas than in the red pine stands. Nitrogen concentration in foliage was higher in L. cuneata (20.28 mg N g^-1) than that in F. arundinacea (5.67 mg N g^-1), whereas potassium concentration was twice as high in F. arundinacea (18.8 mg K g^-1) as that in L. cuneata (9.07 mg K g^-1). Foliar nitrogen concentrations in invasive vegetation such as A. incana, R. pseudoacacia, and Lespedeza spp. were twice or four times higher than those of Q. acutissima and P. koraiensis. Our results indicate the development of suitable vegetation and soil amendment treatments to improve poor soil environmental conditions in restoration areas are necessary following opencast kaolinite mining.

• Korean Journal of Veterinary Research
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• v.63 no.3
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• pp.27.1-27.9
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• 2023

Lespedeza cuneata (LC) is a perennial plant used in herbal medicine to treat numerous diseases, including prostatic hyperplasia, diabetes, early atherosclerosis, and hematuria. Reference collections of bioactive compounds of LC are crucial for the determination of their pharmacological properties. However, little is known regarding its anti-oxidative and anti-inflammatory effects in alveolar macrophage (MH-S) cells. This study examined whether LC can inhibit reactive oxygen species and Coal fly ash (CFA) induced inflammation in MH-S cells. The anti-oxidative effects of LC were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, anti-inflammatory effects were examined using nitric oxide (NO) assay, and cytotoxicity was analyzed using methyl thiazolyl tetrazolium assay. The expression of inflammatory cytokine genes was assessed through a reverse-transcription polymerase chain reaction. Our results revealed that LC exhibited high radical scavenging activity and a dose-dependent (7.8-1,000 ㎍/mL) inhibition of oxidation as compared to ascorbic acid and Trolox. It also inhibited CFA-induced NO production in MH-S cells. Moreover, it suppressed the CFA exposure-mediated expression of pro-inflammatory mediators and cytokines, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. These results suggest that LC is a potent antioxidant and anti-inflammatory agent that can be useful as a nutraceutical product.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.6
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• pp.84-93
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• 2004

This study was carried out to select proper species for early stage replantation in granite forest roads cut-slope. From two to sixteen year elapsed forest roads in four regional areas of Gangwon-do, sample plots were selected, and their vegetations and environmental factors were investigated. Soil organic matter, total nitrogen, soil moisture content, and bulk density in the decomposed granite soils were below 1.32%, 0.08%, 10.0%, and above 1.24g/$cm^3$, respectively. The result indicated that the soil was not good for plant growth. The environmental factors affecting distribution of species in the granite forest roads were the elapsed year, cut-slope, elevation, and cut-slope aspect. Species with high frequency in the granite forest road cut-slope ordered Lespedeza bicolor, Rubus crataegifolius, Pinus densiflora, Lysimachia clethroides, Amorpha fruticosa, Dactylis glomerata, Lespedeza cyrtobotrya, Festuca arundinacea, Spodiopogon sibiricus, Artemisia stolonifera, and Weigela subsessilis. Herbs seeded in earth stage such as Dactylis glomerata, Lolium perenne, and Festuca arundinacea had changed into perennial herbs such as Carex lanceolata, Poa sphondylodes, Patrinia villosa, Artemisia princeps var. orientalis, Spodiopogon sibiricus, Melica onoei, Artemisia keiskeana, Artemisia stolonifera, Astilbe chinensis var. davidii, Spodiopogon sibiricus, and those had changed into shrubs such as Rubus crataegifolius and Weigela subsessilis, and those had changed into trees such as Salix hulteni, Salix koreensis, and Fraxinus rhyncholhylla according to elapsed year of forest roads. Legumes such as Lespedeza sp., Pueraria thunbergiana, Amorpha fruticosa, etc., herbs such as Artemisia keiskeana, Artemisia princeps var. orientalis, Artemisia stolonifera, Astilbe chinensis var. davidii, Spodiopogon sibiricus, Miscanthus sinensis, etc., and woody plants such as Pinus densiflora, Rubus crataegifolius, Weigela subsessilis, Zanthoxylum schinifolium, Rhododendron mucronulatum, etc., with foreign herbs were considered as proper species for replantation in granite forest road cut-slope.

• Journal of Plant Biology
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• v.35 no.4
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• pp.317-322
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• 1992

The nitrogen fixation activity and environmental factors of Lespedeza bicli!oy Turcz, forming annual root nodules by symbiotic Rhizobium sp. were analyzed in the field conditions during the growing period. Seasunal changes of $N_2-fixation$ activity showed the maximum value of $120\;\mu\textrm{M}\;C_2_H4{\cdot}noduie\;g\;fw^{-1}{\cdot}hr^{-1}$ during the active growing period (June) and varied significantly depending on the growth phase and environmental factors. The maximum activities were attained at the conditions of pH 7, $30^{\circ}C$ of temperature, 18 Kpa of oxygen partial pressure and inhibited by water stress and nitrogen sources. The habitat soil was weak acidic and poor in nitrogen, phosphorus and organic matter contents. The leaf area ratios and chlorophyll contents were ranged from 442 to $48;\textrm{cm}^2{\cdot}g\;dw^{-1}$ and from 33 to $38\;\mu\textrm{g}\;chI{\cdot}\textrm{cm}^2$. Nitrogen contents in each organ showed the maximum of 46, 19 and $11\;mg{\cdot}g\;dw^{-1}, respectively for leaf, rool and stem in the early period. The highest phosphorus contents were 4.2, 1.2 and $0.6\;mg{\cdot}g\;dw^{-1}$, respectively for leaf. root and stem in early growing period. The allocation ratios of nitrogen quantity showed 60% for leaves and 73% for roots during the active and late growth period, and 22% [or stems in average. The allocalion ralios o[ phosphorus quantity showed 58% for leaves during the most productive period, 70% for roots in the pre-growth stage and 26% for stems in average.verage.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.2
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• pp.53-62
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• 2002

The objectives of this study were to understand the tolerance mechanism of woody plants to water stress and tolerance changes in relation to mycorrhizal formation. Lespedeza cyrtobotrya Miq. commonly used for erosion control in slopes were raised from seeds and transplanted to 120 plastic pots. Sixty pots received the top soil of a Fraxinus americana forest, while remaining 60 pots received the autoclaved top soil. The forest soil contained 1,200 spores per 100g of arbuscular endomycorrhizal fungus, mostly Glomus sp. The plants were raised outside with regular supply of water and mineral nutrients. Two kinds of water deficit treatment and a control were started at the middle of July : cyclic water deficit treatment with 3 cycles of sequential water stress at the point of xylem water potential of about -0.6, -0.6, and -1.7 MPa and recovery, and non-cyclic water deficit treatment with single water stress at about -1.5 MPa. The non-stressed plants received plenty of water throughout the period. In late August the plants were harvested for measurements of dry weight, N, P, carbohydrate contents, net photosynthesis and superoxide dismutase(SOD) activities. Both cyclic and non-cyclic water deficit treatments reduced dry weight by 60% and 40%, respectively, and reduced nitrogen absorption, while increased SOD activities. Water-stressed plants also showed increased carbohydrate contents in the leaves and lowered stomatal conductance. Mycorrhizal inoculation resulted in an average of 40% infection of roots and 2-3 times increase in P absorption in water-stressed as well as non-stressed plants. Mycorrhizal formation also increased shoot-root ratio. The results that SOD activities of water-stressed plants with mycorrhizal infection were significantly lower than those of non-mycorrhizal plants suggest the possibility of improvement of water-stressed condition by mycorrhizal formation. It was concluded that endomycorrhizal formation increased tolerance of Lespedeza cyrtobotrya seedlings to water stress.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.99-107
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• 2013

This study was conducted to evaluate the effect of soil covering depth and vegetation base materials on the competition between Pinus densiflora Siebold & Zucc. and Lespedeza cyrtobotrya Miq., which were grown in an abandoned coal mine land for three years after seeding, by comparing their growth and stem density. The study site was consisted of sub-plots with four different soil covering depths (0cm, 10cm, 20cm, and 30cm) and four different compounds of vegetation base materials (peat moss (control), soil conditioner+peat moss (S+P), erosion control material+peat moss (E+P), and soil conditioner+erosion control material+peat moss (S+E+P)). Results of this study showed opposite pattern between P. densiflora and L. cyrtobotrya with different soil covering depth and compounds of vegetation base materials in general. P. densiflora showed the highest growth and stem density in plots with 10cm and 0cm depths of soil covering, respectively, while the lowest was shown in plots with 20cm depth of soil covering. In contrast, L. cyrtobotrya showed the highest growth and stem density in plots with 20cm depth of soil covering, while the lowest was shown in plots with 0cm depth of soil covering. In case of vegetation base materials, P. densiflora showed the highest growth and stem density in control plots and plots treated with S+P, respectively, while the lowest was shown in plots with S+E+P treatment. On the other hand, L. cyrtobotrya showed the highest growth and stem density in plots treated with S+E+P, while the lowest was shown in control plots. These results suggested the competition between two plants as a major cause of opposite patterns, which is induced by the suppressed growth and stem density of P. densiflora by fast growing L. cyrtobotrya. Despite the suppression of L. cyrtobotrya on P. densiflora, L. cyrtobotrya can play a positive role in improving soil quality, and thus it would be more desirable for restoring abandoned coal mine land to manage the growth of L. cyrtobotrya periodically, rather than eliminate them.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.1
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• pp.59-67
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• 2004

This study was carried out to select proper species for early stage replantation in granite forest roads cut-slope. From one to fourteen year elapsed forest roads in eight regional areas of Gyeongsang-do and Jeolla-do, sample plots were selected, and their vegetations and environmental factors were investigated. Soil organic matter, total nitrogen, soil moisture content, and bulk density in the granite weathering soils were below 0.4%, 0.03%, 12.4%, and above 1.2g/$cm^3$, respectively. The result indicated that the soil was not good for plant growth. The environmental factors affecting distribution of species in the granite forest roads were the elapsed year, elevation, and cut-slope aspect. Invaded species with high frequency in the forest road cut-slope ordered Pinus densiflora, Lespedeza bicolor, Rubus crataegifolius, Alnus hirsuta, Youngia denticulata, Patrinia villosa, Artemisia princeps var. orientalis, Zanthoxylum schinifolium, and Dactylis glomerata. Herbs seeded in earth stage such as Dactylis glomerata, Eragrostis curvula, Festuca arundinacea, Lespedeza sp., and Amorpha fruticosa had changed into perennial herbs and shrubs such as Youngia denticulata, Patrinia villosa, Aster scaber, Patrinia scabiosaefolia, Melica onoei, Astilbe chinensis var. davidii, Chrysanthemum zawadskii var. latilobum, Pueraria thunbergiana, Stephanandra incisa, and Clematis apiifolia, and had changed into plants such as Artemisia princeps var. orientalis, Artemisia keiskeana, Miscanthus sinensis, Pinus densiflora, Rhododendron mucronulatum, Rubus crataegifolius, and Zanthoxylum schinifolium according to elapsed year of forest roads. Leguma such as Lespedeza sp., Pueraria thunbergiana, Amorpha fruticosa, etc., herbs such as Artemisia keiskeana, Artemisia princeps var. orientalis, Chrysanthemum zawadskii var. latilobum, Miscanthus sinensis, etc., and woody plants such as Pinus densiflora, Rubus crataegifolius, Rhododendron mucronulatum, etc., with foreign herbs were considered as proper species for replantation in granite forest road cut-slope.

• Journal of Korean Society of Forest Science
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• v.31 no.1
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• pp.30-36
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• 1976

In order to guide the project for tree seedling production and direct seeding afforestation by introduction of genus Lespedeza, studies were conducted on the effect of seed storage period and on percentage and rate of germination through various kinds of treatments. L. bicolor, L. cyrtobotrya, and L. japonica. intermedia seed were storeds at different period of time. The results obtained are summarized as follows: 1. Germination capacity was not reduced until 28 months after harvest. However, germination was very poor 40 months after harvest, with less than 30% germination obtained by germination treatments. 2. Removal of seed coat and cone, sulphuric acid treatments were more effective in increasing germination than other treatments. Especially, most seeds germinated one week earlier than other treatments by removal of the seed coat. 3. The seeds stored for a short period of time showed more rapid germination compared to seeds which were stored for long period.

• Journal of the Korean Wood Science and Technology
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• v.45 no.6
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• pp.857-871
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• 2017

The major substrates used in soilless growing media are peat moss and perlite, where peat moss is a limited and expensive natural resource. Determination of appropriate substrates based on technical and economic feasibility is the vital aspect of research and the key to success in any soilless production system. This research work was performed to evaluate different low-cost and sustainable alternative substrate as soilless growing media for horticulture plant. The objective of this study was to compare the effect of sawdust species and steam treatment, for physico-chemical properties and growth of horticultural plant. This study involves the physical and chemical characterization and growth test of four substrate (pine sawdust, oak sawdust, steamed pine sawdust and steamed oak sawdust) in order to evaluate their use as components of growing media. Steamed oak sawdust ($121^{\circ}C$, 30 min) showed adequate physical and chemical properties compared to peat moss for their use as growing media. The growing media were prepared using different mixture proportion to grow Brassica campestris L., Festuca arundinacea and Lespedeza cyrtobotrya Miq. The highest germination, stem length and leaf area of Brassica campestris L., Festuca arundinacea and Lespedeza cyrtobotrya were observed in 30 minute steamed oak sawdust mixture growing media. The steam treatment condition of sawdust used in the growing media significantly positive affected the germination, the stem length and the leaf area.