• Mycobiology
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• 제51권4호
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• pp.239-245
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• 2023

Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the 𝛽-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 ℃ while reaching 61% activity at 65 ℃. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.

• 대한수의학회지
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• 제37권2호
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• pp.339-347
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• 1997

A cytopathogenic virus was isolated from the brain tissues of pig showing ataxia. The biophysical, morphological and serological assay showed that the isolate belongs to a coronavirus. The differential identification of the isolate with monoclonal antibodies against A and X sites of transmissible gastroenteritis virus indicated that the virus has a characteristics of porcine respiratory coronavirus. The RT-PCR on nucleocapsid region of TGEV also showed that the isolate has the same conserved sequence. The diverse pathogenesis of PRCV and its implication in field were discussed.

• 상하수도학회지
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• 제20권1호
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• pp.35-43
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• 2006

Concerns related to protecting, identifying, and isolating of subsystems of a water distribution network have led to the realization of the increased importance of valves in the system. The most important purpose of valves in water distribution systems is to isolate a subsystem due to breakage, maintenance activities, or contamination. A subsystem called segment is isolated by the closure of adjacent valves. Minimizing the pipe failure impact, an efficient algorithm is required to identify adjacent valves quickly. In this paper, an algorithm to identify adjacent valves to be closed to isolate a subsystem from the remainder of a network when a pipe failure is presented. The algorithm is operated on a matrix called the valve location matrix containing the information of valve locations. An application to an existing water distribution system demonstrates the developed algorithm efficiently locates the adjacent valves for the isolation of a broken pipe.

• 한국응용과학기술학회지
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• 제34권1호
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• pp.33-40
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• 2017

To isolate and identify the yeast strains associated with D. morbifera, homogenized D. morbifera root samples were spread onto GPY, DG18, SCG and DOB agar media containing antibiotics, Triton X-100, and l-sorbose. Total 81 yeast isolates were analyzed by sequencing of internal transcribed spacer (ITS) region of the ribosomal DNA. The results showed that the root-associated yeast species were composed of the genera Vanderwaltozyma (40 isolates), Cryptococcus (40 isolates), and Kluyveromyces (one isolate). Moreover, the Kluyveromyces isolate exhibited high bioethanol productivity. In addition, the Vanderwaltozyma and Cryptococcus were dominant in D. morbifera roots. The specific yeast community associated with D. morbifera roots was identified by phylogenetic sequence analyses. These yeast isolates may have industrial applications as biosurfactant and bioethanol.

• 한국식품저장유통학회지
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• 제8권2호
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• pp.181-186
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• 2001

The objective of this study is to determine the effect of soy protein isolate (SPI) film coating on the color, firmness, viscosity and weight loss of Rice Cake stored at 15, 20 and $25^{\circ}C$ (RH 50%) for 30 days. Raw materials mixed with SPI and cocoa powder (10:0, 7:3, 5:5, 3:7, w/w) were prepared. After adding sugar and shortening to raw materials, the mixture were refined to 25 micron of particle size. Coating of Rice Cake were carried out at $65^{\circ}C$. SPI coated Rice Cake had higher internal and external firmness comparing to the control at $25^{\circ}C$. SPI coated Rice Cake showed smooth surface morphology and had 0.71-1.01 mm of thickness. SPI coated Rice Cake showed less weight loss for 30 days compared to controls. SPI coating solution was successfully coated on Rice Cake and extended shelf-life over 15 days at room temperature.

• 한국작물학회지
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• 제51권2호
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• pp.148-153
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• 2006

Effects of ${\alpha}$-chymotrypsin modification on degree of hydrolysis (DH), solubility, emulsifying capacity and thermal aggregation of laboratory-purified soy protein isolate (SPI) using a lipoxygenase-defected soybean (Jinpum-kong) and commercial soy protein isolate (Supro 500E) were compared. SPIs were hydrolyzed by ${\alpha}$-chymotrypsin at pH 7.8 and $37^{\circ}C$ for 30 min. DHs of Supro 500E and Jinpum-kong SPI were increased by ${\alpha}$-chymotrypsin modification, and DH of Supro 500E was higher than that of Jinpum-kong SPI. DH of ${\alpha}$-chymotrypsin treated Jinpum-kong SPI was similar with untreated Supro 500E and DH of treated Supro 500E was the highest. Solubility, emulsifying capacity and thermal aggregation of SPIs were increased by ${\alpha}$-chymotrypsin modification, and these changes were highly related to changes in DH. Functional properties of Supro 500E were higher than Jinpum-kong SPI in both of untreated and ${\alpha}$-chymotrypsin treated SPIs.

• 한국균학회지
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• 제33권2호
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• pp.92-94
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• 2005

동양란에 썩음병을 일으키는 병원균을 분리하여 동정하였다. 병원균은 배양학적으로나 형태학적으로 Fusarium 속의 특성을 지니고 있었고, PCR 기법을 사용한 18S rRNA 염기서열을 분석결과 Fusarium 속의 균류와 매우 높은 유사도를 보였다. 계통학적 분석결과, 본 균주는 Fusarium sp. LP-A2/3(AF548314)과 가장 근접한 것으로 나타났다.

• 한국응용곤충학회지
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• 제19권1호
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• pp.11-20
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• 1980

특정수도품종에 대한 도열병균의 침입률은 접종전처리온도와 균주의 조합보다는 공시균주나 온도의 단독요인에 의한 영향이컸다. 이병성품종은 그 품종이 어떤 온도조건하에서 자랐던지 또는 병원성이 다른 어떤 균주로 접종이 되었던지 이병성으로 남아 있었으며 접종전에 기주가 다른 온도조건하에서 자랐을 경우, 도열병균의 특정균주 또는 Race의 병원성발현은 다르게 나타나는 것이 관찰되었다.

• 한국식생활문화학회지
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• 제14권5호
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• pp.467-476
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• 1999

We studied to improve the functional properties of soybean protein isolate by dimethylglutarylation and acetylation. Soybean protein isolate was acylated rapidly up to 80% modification and more of 80% modification was proceed slowly. Electrophoretic analysis showed that more changes in modified protein. Also, modification of soybean protein produced more ionizable tyrosines and exposed more hydrophobic groups, while modified protein exhibited a loss of reactive sulfhydryl groups. Spectrophoretic studies demonstrated that the shift was occurred at the UV 278nm and fluorescence 333nm, respectively, and the intensity decreased as the degree of modification was increased.

• 한국균학회지
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• 제18권1호
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• pp.20-25
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• 1990

Fructosyl transferase의 생산성이 높은 미생물을 얻기 위해 자연계에서 폭넓은 검색을 수행하였다. 300점 이상의 미생물을 분리하고 각각의 효소의 활성을 측정하였다. 그 결과 커피 자동판매기로부터 분리한 C23번 분리균은 다른 분리균들과 비교하여 높은 효소의 활성을 나타내어 fructosyl transferase 생산균으로 선발되었다. 다양한 배지에서 C23 분리균을 배양하여 형태, 그리고 배양특징 등을 연구하여 Aureobasidium pullulans var. melanigenum으로 동정하였다.